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A high cellulase-producing bacterial isolate TS4 was recovered from an Egyptian soil sample and identified using 16S rRNA gene sequencing as Streptomyces thermodiastaticus. One-factor-at-a-time (OFAT) preliminary studies were carried out to determine the key factors affecting cellulase production by S. thermodiastaticus and their optimum ranges. The initial pH of the medium, carboxymethyl cellulose (CMC), tryptone, and NaCl concentrations were further optimized using a response surface Central Composite design. Fermentation under optimized variables of initial pH 6.0, presence of CMC, tryptone, and NaCl at concentrations of 2%, 0.03%, and 0.12%, respectively, resulted in 3.24 fold increase in cellulase productivity (2023 U/L) as compared to that under basal conditions (625 U/L). Cellulase production was also improved with a 4 Kilogray (KGy) dosage of gamma radiation. In comparison to the wild-type strain under basal circumstances, S. thermodiastaticus produced 5.1 fold more cellulase after a combination of model-based optimization and gamma radiation mutation. Cellulase was partially purified using ammonium sulfate precipitation followed by dialysis. The resulting cellulase was 1.74 times purified and its specific activity was 4.21 U/mg. The molecular weight of cellulase is 63 kDa as indicated by SDS-PAGE and zymogram. Its maximum activity was achieved at 60 °C and pH 5.0. In addition, it showed outstanding thermo-tolerance as it could retain its full activity after a 12-h incubation at 90 °C. [ABSTRACT FROM AUTHOR]

In the present study we reported, for the first time, the gamma irradiation induced synthesis of chitosan/Au/bioactive glass (CS/Au/BG) nanocomposite. The bioactive glass (BG), with the composition 45% SiO2, 32.5% CaO, 15% Na2O, and 7.5% P2O5 wt% was synthesized through the sol–gel technique. XRD, SEM, EDX, and elemental mapping images were utilized to evaluate the structure of pure BG and CS/Au/BG nanocomposite. The antimicrobial efficacy was evaluated by zone of inhibition (ZOI), minimum inhibitory concentration (MIC), growth curve assay, and Ultraviolet irradiation effect. Investigation was carried on the antibiofilm effectiveness. Membrane leakage as well as SEM imaging were used to evaluate the antibacterial reaction mechanism. The crystallite size of CS/Au/BG nanocomposite was determined via Scherer equation as 22.83 nm. CS/Au/BG possessed the most ZOI activity against the tested microbes. The highest inhibition % of BG, and CS/Au/BG nanocomposite was investigated for S. aureus (15.65%, and 77.24%), followed by C. albicans (13.32%, and 64.75%). The quantity of protein leakage was directly-proportional after increasing the concentration of BG, and CS/Au/BG and counted to be 70.58, and 198.25 µg/mL, respectively (after applied 10 mg/mL). The promising results suggested the use of novel CS/Au/BG nanocomposite as an encourage candidate for wastewater treatment application against pathogenic microbes. [ABSTRACT FROM AUTHOR]