1. Expression of Human Metallothionein III and Its Metalloabsorption Capability in Escherichia coli.
- Author
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Zheng, Wei‐Juan, Wu, Fang, Zhuang, Hong‐Qin, Lu, Chun, Yang, Feng, Ma, Wen‐Li, and Hua, Zi‐Chun
- Subjects
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METALLOTHIONEIN , *ESCHERICHIA coli , *GENE expression , *GLUTATHIONE transferase , *PROTEINASES , *BACTERIAL growth - Abstract
Human metallothionein III (MT III) gene was synthesized with Escherichia coli preference codon usage and expressed in E. coli in glutathione-S-transferase (GST) fusion form. The recombinant MT III was released by proteinase Factor Xa digestion and purified with the yield of 2 mg/L culture, and its specific Cd2+ binding capability was confirmed. E. coli strain BL21(DE3), expressing MT III, showed metal tolerance between 0.1 and 0.5 mM Cd2+ and bacterial growth was inhibited at 1 mM Cd2+. MT III expressing E. coli strain showed binding discrimination between different metal ions in combination use, with the preference order of Cd2+ > Cu2+ > Zn2+. It absorbed different metal ions with relatively constant ratio and showed a cumulative absorption capability for mixed heavy metals. [ABSTRACT FROM AUTHOR]
- Published
- 2004
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