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Start Over Topic fusarium oxysporum
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2. α-Fucosidases with different substrate specificities from two species of Fusarium

Author

Jonathan D. Walton, Janet M. Paper, John S. Scott-Craig, Mareike Bongers, Richard E. Wiemels, Ahmed Faik, David Cavalier, and Melissa S. Borrusch

Subjects
alpha-L-Fucosidase, Genetics, Subfamily, Sequence Homology, Amino Acid, Sequence analysis, Molecular Sequence Data, Fungal genetics, food and beverages, Sequence alignment, Sequence Analysis, DNA, General Medicine, Biology, biology.organism_classification, Applied Microbiology and Biotechnology, Substrate Specificity, Pichia pastoris, Microbiology, Fusarium, Fusarium oxysporum, Glycosides, DNA, Fungal, Sequence Alignment, Gene, Trichoderma reesei, Biotechnology
Abstract

Two fungal-secreted α-fucosidases and their genes were characterized. FoFCO1 was purified from culture filtrates of Fusarium oxysporum strain 0685 grown on l-fucose and its encoding gene identified in the sequenced genome of strain 4287. FoFCO1 was active on p-nitrophenyl-α-fucoside (pNP-Fuc), but did not defucosylate a nonasaccharide (XXFG) fragment of pea xyloglucan. A putative α-fucosidase gene (FgFCO1) from Fusarium graminearum was expressed in Pichia pastoris. FgFCO1 was ∼1,800 times less active on pNP-Fuc than FoFCO1, but was able to defucosylate the XXFG nonasaccharide. Although FgFCO1 and FoFCO1 both belong to Glycosyl Hydrolase family 29, they share

Published
2012