6 results on '"Li, Guotian"'
Search Results
2. Efficient approaches for generating GFP fusion and epitope-tagging constructs in filamentous fungi.
- Author
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Zhou X, Li G, and Xu JR
- Subjects
- Chromatography, Affinity, Fungal Proteins genetics, Fungal Proteins metabolism, Fungi genetics, Genetic Vectors, Genome, Fungal, Genomics methods, Green Fluorescent Proteins genetics, Immunoprecipitation, Magnaporthe genetics, Magnaporthe metabolism, Oryza microbiology, Recombinant Fusion Proteins genetics, Saccharomyces cerevisiae genetics, Saccharomyces cerevisiae metabolism, Subcellular Fractions metabolism, Epitopes genetics, Fungi metabolism, Gene Targeting methods, Green Fluorescent Proteins metabolism, Recombinant Fusion Proteins metabolism
- Abstract
For functional characterization of predicted genes encoding hypothetical proteins in fungal genomes, it is complementary to genetic studies to determine their expression and subcellular localization patterns in different developmental or infection stages. It is also important to identify and characterize other proteins that are physically associated with or functionally related to these genes in vivo by co-immunoprecipitation or affinity purification analyses. In this chapter, we described a set of yeast shuttle vectors and protocols to generate fusion constructs by the yeast gap repair approach. Because of the simplicity and efficiency of yeast gap repair, these vectors and the general methods described in this chapter are suitable for functional genomics studies in filamentous fungi.
- Published
- 2011
- Full Text
- View/download PDF
3. Roles of Rack1 Proteins in Fungal Pathogenesis.
- Author
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Zhang, Xue, Jain, Rashmi, and Li, Guotian
- Subjects
ASPERGILLUS ,CANDIDA albicans ,CELL receptors ,CYCLIC adenylic acid ,BIOLOGICAL evolution ,FUNGI ,GENETICS ,MYCOSES ,PROTEIN kinases ,PROTEINS - Abstract
Pathogenic fungi cause diseases on various organisms. Despite their differences in life cycles, fungal pathogens use well-conserved proteins and pathways to regulate developmental and infection processes. In this review, we focus on Rack1, a multifaceted scaffolding protein involved in various biological processes. Rack1 is well conserved in eukaryotes and plays important roles in fungi, though limited studies have been conducted. To accelerate the study of Rack1 proteins in fungi, we review the functions of Rack1 proteins in model and pathogenic fungi and summarize recent progress on how Rack1 proteins are involved in fungal pathogenesis. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
4. Activation of the signalling mucin MoMsb2 and its functional relationship with Cbp1 in M agnaporthe oryzae.
- Author
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Wang, Guanghui, Li, Guotian, Zhang, Shijie, Jiang, Cong, Qin, Jun, and Xu, Jin ‐ Rong
- Subjects
- *
MUCINS , *CELLULAR signal transduction , *MITOGEN-activated protein kinases , *ENZYME activation , *APPRESSORIA , *ASCOMYCETES , *FUNGI - Abstract
Various surface signals are recognized by M agnaporthe oryzae to activate the Pmk1 MAP kinase that is essential for appressorium formation and invasive growth. One of upstream sensors of the Pmk1 pathway is the MoMsb2 signalling mucin. However, the activation of MoMsb2 and its relationship with other sensors is not clear. In this study, we showed that the cleavage and transmembrane domains are essential for MoMsb2 functions. Cleavage of MoMsb2 was further confirmed by western blot analysis, and five putative cleavage sites were functionally characterized. Expression of the extracellular region alone partially rescued the defects of M omsb2 in appressorium formation and virulence. The cytoplasmic region of MoMsb2, although dispensable for appressorium formation, was more important for penetration and invasive growth. Interestingly, the M omsb2 cbp1 double mutant deleted of both mucin genes was blocked in Pmk1 activation. It failed to form appressoria on artificial surfaces and was non-pathogenic. In addition, we showed that MoMsb2 interacts with Ras2 but not with MoCdc42 in co-immunoprecipitation assays. Overall, results from this study indicated that the extracellular and cytoplasmic regions of MoMsb2 have distinct functions in appressorium formation, penetration and invasive growth, and MoMsb2 has overlapping functions with Cbp1 in recognizing environmental signals for Pmk1 activation. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
5. The MADS-box transcription factor FgMcm1 regulates cell identity and fungal development in F usarium graminearum.
- Author
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Yang, Cui, Liu, Huiquan, Li, Guotian, Liu, Meigang, Yun, Yingzi, Wang, Chenfang, Ma, Zhonghua, and Xu, Jin ‐ Rong
- Subjects
TRANSCRIPTION factors ,FUNGAL development ,GENETIC regulation ,FUSARIUM ,WHEAT diseases & pests ,FUNGAL mutation ,FUNGI - Abstract
In eukaryotic cells, MADS-box genes are known to play major regulatory roles in various biological processes by combinatorial interactions with other transcription factors. In this study, we functionally characterized the FgMCM1 MADS-box gene in F usarium graminearum, the causal agent of wheat and barley head blight. Deletion of FgMCM1 resulted in the loss of perithecium production and phialide formation. The F gmcm1 mutant was significantly reduced in virulence, deoxynivalenol biosynthesis and conidiation. In yeast two-hybrid assays, FgMcm1 interacted with Mat1-1-1 and Fst12, two transcription factors important for sexual reproduction. Whereas F gmcm1 mutants were unstable and produced stunted subcultures, F gmcm1 mat1-1-1 but not F gmcm1 fst12 double mutants were stable. Furthermore, spontaneous suppressor mutations occurred frequently in stunted subcultures to recover growth rate. Ribonucleic acid sequencing analysis indicated that a number of sexual reproduction-related genes were upregulated in stunted subcultures compared with the F gmcm1 mutant, which was downregulated in the expression of genes involved in pathogenesis, secondary metabolism and conidiation. We also showed that culture instability was not observed in the F vmcm1 mutants of the heterothallic F usarium verticillioides. Overall, our data indicate that FgMcm1 plays a critical role in the regulation of cell identity, sexual and asexual reproduction, secondary metabolism and pathogenesis in F . graminearum. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
6. Genome sequence of the model rice variety KitaakeX
- Author
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Rashmi Jain, Kerrie Barry, Jane Grimwood, Wendy Schackwitz, Pamela C. Ronald, Jerry Jenkins, David Dilworth, Guotian Li, Jeremy Schmutz, Joel Martin, Jayson Talag, Catherine R. Nelson, Diane Bauer, Dario Copetti, Rod A. Wing, David Kudrna, Feng Xing, Weibo Xie, Anna Lipzen, Shengqiang Shu, Nikki T Pham, Mawsheng Chern, Phat Q. Duong, University of Zurich, and Li, Guotian
- Subjects
0106 biological sciences ,Kitaake ,Sequence assembly ,Medical and Health Sciences ,01 natural sciences ,Genome ,Whole genome sequence ,2. Zero hunger ,0303 health sciences ,Contig ,food and beverages ,Genomics ,Biological Sciences ,Phenotype ,1305 Biotechnology ,590 Animals (Zoology) ,Functional genomics ,Genome, Plant ,Research Article ,Biotechnology ,KitaakeX ,lcsh:QH426-470 ,Bioinformatics ,lcsh:Biotechnology ,Computational biology ,XA21 immune receptor ,Biology ,Oryza ,DNA sequencing ,10127 Institute of Evolutionary Biology and Environmental Studies ,03 medical and health sciences ,1311 Genetics ,Information and Computing Sciences ,lcsh:TP248.13-248.65 ,Genetics ,Gene ,030304 developmental biology ,Whole genome sequencing ,Oryza sativa ,Whole Genome Sequencing ,Zhenshan97 ,Nipponbare ,fungi ,Human Genome ,Computational Biology ,Genetic Variation ,Molecular Sequence Annotation ,Plant ,biology.organism_classification ,lcsh:Genetics ,570 Life sciences ,biology ,Rice ,De novo genome assembly ,Reference genome ,010606 plant biology & botany - Abstract
Background: The availability of thousands of complete rice genome sequences from diverse varieties and accessions has laid the foundation for in-depth exploration of the rice genome. One drawback to these collections is that most of these rice varieties have long life cycles, and/or low transformation efficiencies, which limits their usefulness as model organisms for functional genomics studies. In contrast, the rice variety Kitaake has a rapid life cycle (9 weeks seed to seed) and is easy to transform and propagate. For these reasons, Kitaake has emerged as a model for studies of diverse monocotyledonous species. Results: Here, we report the de novo genome sequencing and analysis of Oryza sativa ssp. japonica variety KitaakeX, a Kitaake plant carrying the rice XA21 immune receptor. Our KitaakeX sequence assembly contains 377.6 Mb, consisting of 33 scaffolds (476 contigs) with a contig N50 of 1.4 Mb. Complementing the assembly are detailed gene annotations of 35,594 protein coding genes. We identified 331,335 genomic variations between KitaakeX and Nipponbare (ssp. japonica), and 2,785,991 variations between KitaakeX and Zhenshan97 (ssp. indica). We also compared Kitaake resequencing reads to the KitaakeX assembly and identified 219 small variations. The high-quality genome of the model rice plant KitaakeX will accelerate rice functional genomics. Conclusions: The high quality, de novo assembly of the KitaakeX genome will serve as a useful reference genome for rice and will accelerate functional genomics studies of rice and other species., BMC Genomics, 20, ISSN:1471-2164
- Published
- 2019
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