1. Intestinal permeability of antivirus constituents from the fruits of Eucalyptus globulus Labill. in Caco-2 Cell Model.
- Author
-
Yang XW, Guo QM, Wang Y, Xu W, Tian L, and Tian XJ
- Subjects
- Alkylating Agents chemistry, Antiviral Agents isolation & purification, Biological Availability, Caco-2 Cells, Calcium Channel Blockers pharmacology, Cell Membrane metabolism, Chemical Phenomena, Chemistry, Physical, Chromatography, High Pressure Liquid, Humans, Intestinal Absorption, Iodoacetamide pharmacology, Phloroglucinol analogs & derivatives, Phloroglucinol chemistry, Phloroglucinol pharmacokinetics, Propionates pharmacology, Quinolines pharmacology, Sesquiterpenes chemistry, Sesquiterpenes pharmacokinetics, Verapamil pharmacology, Antiviral Agents pharmacokinetics, Eucalyptus chemistry, Fruit chemistry
- Abstract
The uptake and transepithelial transport of the three main constituents macrocarpal A (M-A), macrocarpal B (M-B), and cypellocarpa C (Cy-C) from the fruits of Eucalyptus globulus Labill. were investigated. Monolayers of the human intestinal epithelial cancer cell line Caco-2 were incubated with M-A, M-B, and Cy-C to model its intestinal absorption and transport, respectively. The determination of compounds was performed by HPLC. The apparent permeability coefficients (P(app)) for M-A, M-B, and Cy-C in the apical-to-basolateral direction of a Caco-2 monolayer were (1.70+/-0.06)x10(-6), (1.99+/-0.10)x10(-6), and (6.08+/-0.41)x10(-6)cm/s, respectively. In the presence of iodoacetamide, the P(app) of Cy-C were both reducted in apical-to-basolateral and basolateral-to-apical directions. M-A and M-B appear to accumulate in the epithelial cells. The intestinal absorption of M-A, M-B, and Cy-C was passive diffusion as the dominating process and Cy-C was partly ATP-dependent.
- Published
- 2007
- Full Text
- View/download PDF