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Melinjo (Gnetum gnemon L.) peel has been known as antioxidant, antibacterial, and antifungal, but not yet used as cosmetic ingredients or drugs optimally. This research focuses on the production of organic soap bars from melinjo peel extract, the quality testing, and antimicrobial analysis against selected microbes. The soap making process was done by the saponification reaction between the mixture of palm oil and coconut oil with sodium hydroxide through cold and hot processes. Distilled water and ethanol were used as solvents for melinjo peel extraction. The quality test was done by determining insoluble fraction in ethanol, free alkali, and unsaponified fatty matter. An antimicrobial activity test was conducted using the Kirby-Bauer diffusion method. The result shows that the quality test fit with INS 2016 for all the criteria except for unsaponified fatty acid in the cold process soap bar. Inhibition zone of organic soap bar sequentially decreased against the growth of Tricophytone mentagrophytes, Staphylococcus aureus, and Staphylococcus epidermidis. Therefore, the organic soap bar with melinjo peel extract shows a good potential to be used as an antimicrobial soap.

Citrullus colocynthis (C. colocynthis) seed is a common and expensive cuisine in West African diet unlike the seeds from Citrullus lanatus (C. lanatus) that are agro waste. The oils from these seeds were comparatively profiled using their physicochemical properties, fatty acids and phenolic contents, in-vitro antioxidant capacity (DPPH, TEAC, FRAP, ORAC), inhibitions of lipid peroxidation and tyrosinase. Toxicities of the oils in mice were also assessed using plasma AST, ALT, cholesterol, HDL-C, liver, heart and kidney (GSH, GPx and SOD) and clastogenic effects in mouse bone marrow. Citrullus lanatus oil contains higher peroxides, saponification, saturated fatty acids and stearic acid (6-fold) levels, with a lower acid value when compared to C. colocynthis. Strong antioxidant capacity, inhibition of lipid peroxidation and tyrosinase by C. lanatus oil extract is proposed to be related to the presence of catechins that are absent in C. colocynthis. The in-vivo study indicated that C. lanatus induced significant increase in ALT while a significant increase of AST was observed in the C. colocynthis treated group. Citrullus lanatus induced a significantly (p 0.05). This work reports for the first time the presence of stearic acid and catechin in C. lanatus oil which is important for its compelling in vitro activity. High doses of both oils could evidently induce toxicological indices in mice.

The increased cost of biodiesel production from edible oils has motivated the researchers to find alternative non-edible oil feedstocks. Jatropha curcas has been identified as a promising tropical and subtropical perennial energy crop for biodiesel production. However, securing adequate J. curcas oil for sustainable biodiesel production is not achieved yet. Therefore, the present study sought to investigate the potential and suitability of J. curcas grown at different areas of Ethiopia for biodiesel production using analytical, instrumental, and empirical methods. The dry weight proportion of J. curcas seeds, seed coats, and kernels were significantly varied across growing ecologies. The oil contents of kernels were reneged between 47.10–59.32%, while the free fatty acid content of all sampled oils was less than 2.7%. The estimated iodine and saponification values were ranged between 99.6–112.6 mg I 2 and 180.9–202.0 mg KOH per gram of oil, respectively. Oleic (34.2–42.2%) and Linoleic (34.8–41.8%) acids dominated the fatty acid composition of J. curcas oil. The biodiesel fuel properties such as kinematic viscosity, density, cold filter plugging point, and cetane numbers predicted from the fatty acid composition of oils were agreed with EN-14214 standards. The k-means cluster revealed that kernels harvested from different geographic locations were grouped in the same cluster, which indicates that the effect of altitudinal variation on the oil content of J. curcas was not significant. However, at too lower and higher altitudes, the kernel’s oil content has significantly declined. In conclusion, Ethiopian variety J. curcas oil showed suitable physicochemical properties for biodiesel production. However, its yield, composition, and physicochemical properties were significantly varied across growing areas.

Herein, the current investigation presents the study of two novel methods namely saponification liquid–liquid extraction coupled TLC (Sp-LLE-TLC) and direct liquid–liquid microextraction (LLME). These two methods were developed for enhanced determination of vitamin D3 in the fortified sunflower oil prior HPLC–UV analysis. By employing the proposed methods, linear dynamic range of 0.1–10 µg m/L with high determination coefficient of R2 > 0.94 was achieved in this work. Moreover, the obtained limit of detection and recovery values were found to be in reasonable range of 0.03–0.1 µg m/L and 71.7–101.44% (RSDs, 1.2–6.1%), respectively. Finally, by comparing two methods, the LLME was found to be green (organic solvent 500 µL), fast, and economic method for determination of vitamin D3 in the fortified sunflower oil as compared to Sp-LLE-TLC. Thus, the current work proposed a facile, economic, and green method for fast estimation of vitamin D3 in the fortified sunflower oil.

Functional food is a food ingredient in addition to basic needs as nutrients that can also play a functional role in health. This research aimed to determine the physicochemical characteristics and fatty acid composition of catfish oil, red palm oil, and shark liver oil as functional food ingredients. The research method was to extract fish oil from belly flap, purify catfish oil, and process red palm oil (RPO) from crude palm oil (CPO). The analysis parameters consisted of sensory analysis, oil chemical characteristics (free fatty acid analysis, peroxide, iodine, saponification, and acid numbers), total carotene, tocopherol, and analysis of fatty acid composition. The results showed that the catfish oil after being purified had sensory characteristics, smelled slightly fishy and semi-solid, and had a bright yellow color. The results of the analysis of chemical characteristics showed that the free fatty acid numbers of catfish oil and shark liver oil were following IFOS standards (1.33 and 0.62%), and the RPO numbers for peroxide and free fatty acids according to the SNI standards (9.56 meq kg and 1.44%). The highest ω-3 and ω- 6 fatty acids were in shark liver oil (3.56 and 35.35%), followed by catfish oil (1.72 and 19.9%). and RPO does not contain ω-3 and ω-6. Catfish oil, RPO, and shark liver oil act as functional foods. The fatty acid composition of catfish, shark liver and red palm oil contains saturated and the fatty acid composition of catfish, shark liver and red palm oil contains saturated and unsaturated fatty acids. Mono and poly unsaturated fatty acid (FUFA anf MUFA) in crude catfish oil, pure catfish oil, shark liver oil, and red palm oils were 56.71, 58.12, 63.81 and 47.39% respectively. The result of analysis showed composition of in catfish oil 1.72 and 19.9 %. The content of and of shark liver oil was 3.5 and 35.5%. Whereas in red palm oil does not Ω 3 and Ω 6. The content of EPA and DHA in shark liver oil was 0.08, 0.09 but not in catfish and red palm oil. The total content of carotene and tocopherol in red palm oil was 513.86 and 925.80 mg/kg, respectively. The nutritional composition of catfish oil, red palm oil, and shark liver oil has the potential to be used as functional food. Keywords:Characteristic physicochemicalCaroteneTocopherolω-3ω-6

Physicochemical and thermal characteristics of Moringa oleifera seed oil were studied. The refractive index of Moringa oleifera seed oil at ambient temperature was 1.46. The acid, peroxide, saponification, and iodine values content of Moringa oleifera seed oil were 2.35 g KOH/kg oil, 7.2 meq O2/kg oil, 189.84 g KOH/kg oil, and 66.78 g I2 /100 g oil, respectively. Moringa oleifera seed oil contains ten fatty acids. Among 10 kinds of fatty acids in Moringa oleifera seed oil, oleic acid is the most abundant fatty acid comprising 70.85% of total oil followed by palmitic acid (8.98%). The unsaturated fatty acids represented by oleic acid are mainly distributed in the Sn-2 position, which is as high as 95.59%. It contains a high level of unsaturated fatty acids. Moringa oleifera seed oil contains 233.29 mg/kg α-tocopherol, 9.04 mg/kg β-tocopherol, and 10.13 mg δ-tocopherol, respectively. Determination of 10 elements in Moringa oleifera seed oil by ICP-MS. Among them, calcium has the highest content. Moringa oleifera seed oil remained stable up to 305 °C with a negligible degradation. Moringa oleifera seed oil has high nutritional value and broad development prospect. This research provides a prospect for the comprehensive development and utilization of Moringa oleifera seed oil. The basic physical and chemical properties and structure of Moringa oleifera seed oil was analyzed and characterized

The influence of a variety of cooking methods (poaching, boiling, grilling (charcoal or gas)), frying (pan, deep frying, and stir frying) with a variety of oils (vegetable oil, extra virgin olive oil, sesame oil, extra light olive oil, and sunflower oil), microwaving, and oven roasting on polycyclic aromatic hydrocarbons (PAHs) formation in rabbit meat samples was investigated. Meat samples (including three replicates) were prepared without additives or spices. PAHs extraction was carried out by saponification method with potassium hydroxide in methanol which was followed by a silica gel column technique and the samples were quantified by using gas chromatography with mass spectrometry (GC‐MS). PAHs standards, fluorene, naphthalene, anthracene, phenanthrene, pyrene, acenaphthalene, fluoranthene, and benzopyrene, were used for this study. The other PAHs except fluorene were not observed (detection limit‐0.009 µg/g) in all the samples. Among traditional processing techniques, higher PAH contents were observed as a result of frying. Frying with vegetable oil produced higher fluorene content (0.06–0.13 µg/g) in the deep‐fried sample, although sesame oil is the best oil which produces lowest PAH contents in fried samples. Among all the processing techniques, lower fluorene (0.01–0.02 µg/g) content was noticed in poaching. Benzo(a)pyrene was not observed in all the investigated samples which is viewed as a reliable strategy of the cooking process for human consumption. After processing, the cooking loss was determined and oven roasting and grilling exhibited greater moisture loss., It was difficult to compare our results with the previous studies because there is no such type of literature in rabbit meat by using a variety of processing treatments. The effect of diversified cooking techniques, a variety of oils, and temperature are important factors for controlling PAHs concentration in meat.

Sabun transparan dapat dibuat dari minyak nabati dengan basa alkali melalui reaksi saponifikasi dengan penambahan transparent agent. Perbedaan karakter bahan dari minyak kelapa ( Cocos nucifera ) dan minyak ayam ( Gallus domesticus ) berpotensi menghasilkan sabun transparan yang unik. Kavitasi gelombang ultrasonik menghomogenkan campuran dan mempercepat laju reaksi. Tujuan penelitian ini membuat sabun transparan dari minyak kelapa dan minyak ayam serta membandingkan sabun yang dihasilkan menggunakan gelombang ultrasonik dan dengan sabun dari metode pemanasan. Penelitian eksperimental dilakukan dengan tahapan preparasi dan karakterisasi minyak kelapa dan minyak ayam, dilanjutkan dengan saponifikasi dengan disertai gelombang ultrasonik. Karakterisasi sabun transparan hasil sintesis menunjukkan bahwa sabun yang terbuat dari minyak kelapa lebih transparan dari yang berasal minyak ayam, dan sabun yang dibuat dengan gelombang ultrasonik lebih transparan dari pada yang dibuat dengan pemanasan. Karakter sabun transparan dari minyak kelapa dengan ultrasonik memiliki kadar air sebesar 22,02%, fraksi tak tersabunkan sebesar 1,01%, bagian tak larut dalam alkohol sebesar 1,79%, alkali bebas sebesar 0,04%, pH 9,35, karakter ini sesuai dengan SNI sabun kecuali kadar airnya. Sabun dari minyak ayam memiliki kadar air 23,26%, fraksi tak tersabunkan sebesar 5,57%, bagian tak larut dalam alkohol sebesar 4,69%, alkali bebas sebesar 0,12%, pH 9,60, dan karakter ini kurang sesuai dengan SNI sabun. Sabun yang dihasilkan dengan metode ultrasonik dengan bahan minyak kelapa memiliki karakter yang paling baik dan memiliki kesesuaian paling banyak dengan SNI sabun padat. Transparent soap is synthesized from vegetable oils with alkaline bases through the saponification reaction with the addition of a transparent agent. The different material characteristics of coconut oil ( Cocos nucifera ) and chicken oil ( Gallus domesticus ) have the potential to produce unique transparent soap. Ultrasonic wave cavitation homogenizes the mixture and accelerates the reaction rate. The purpose of this research is to synthesize transparent soap from coconut oil and chicken oil and compared the soap produced using ultrasonic waves and with soap from the heating method. This experimental laboratory research was carried out with the stages of preparation and characterization of coconut oil and chicken oil, followed by saponification of coconut oil and chicken oil accompanied by ultrasonic waves. Characterization of the synthesized transparent soap. The results in this study indicate that soaps made from coconut oil are more transparent than those made from chicken oil, and soaps made with ultrasonic waves are more transparent than those made by normal heating. The ultrasonic transparent soap character of coconut oil has a moisture content of 22.02%, the non-saponified fraction 1.01%, insoluble part of alcohol by 1.79%, free alkaline by 0.04%, pH 9.35, and this is in accordance with SNI for soap except for its water content. Meanwhile, ultrasonic soap from chicken oil has a moisture content of 23.26%, non-saponified fraction 5.57%, the insoluble portion of alcohol was 4.69%, free alkaline 0.12%, pH 9.60, this is not in accordance with SNI soap. The soap produced by the ultrasonic method with coconut oil has the best character and has the most compatibility with SNI for solid soap.

Vitamin E concentrate was produced through saponification of palm fatty acid distillates (PFAD) and magnesium oxide to form Mg-PFAD, followed by three-stages vitamin E extraction with isopropanol, hexane, or ethanol. The vitamin E-rich extracts were evaporated to remove solvent and produced vitamin E concentrate. The objectives of this research were to investigate the effect of organic solvent’s types and solvent to Mg-PFAD mass ratios on vitamin E concentration, solvent selectivity, and antioxidant activity of the vitamin E concentrate. Vitamin E concentrates obtained after isopropanol extraction had vitamin E concentration of 784 ppm with vitamin E recovery of 16 mg tocopherol/100 mg tocopherol in Mg-PFAD, while vitamin E concentrates obtained after hexane extraction had vitamin E concentration of 574 ppm with vitamin E recovery of 35 mg tocopherol/100 mg tocopherol in Mg-PFAD. Isopropanol extraction produced vitamin E concentrate with the highest selectivity for vitamin E and the highest antioxidant activity of 79% IC. It was found that vitamin E concentration was not proportional to the antioxidant activity of the vitamin E concentrate.Keywords: Direct solvent extraction, palm fatty acid distillate, saponification, vitamin E, unsaponifiable matter

Soap is sodium or potassium salt of fatty acid produced by saponification reaction using sodium or potassium hydroxide. It is used for cleaning, bathing, and washing. Soaps were produced from neem seed oil and sheabutter oil using the cold process method with slight modification. The physicochemical properties (acid value, saponification value, peroxide value, iodine value and moisture content) of the oil were determined and it showed that the values are within the standard specifications. The quality of the soaps were also assessed by determining the physicochemical properties such as; free caustic alkali, matter insoluble in alcohol (MIA), pH, total fatty matter (TFM), cleaning properties, washing properties and foam stability. All the results obtained showed that the soaps analyzed are good soaps safe for the skin.

nut oil was extracted from (black, brown and yellow) cultivars using n-hexane. Physiochemical properties (refractive index, specific gravity, acid value, free fatty acid, peroxide, saponification and iodine values of the oil samples were determined. Storage studies on the tiger nut oils were done by monitoring changes in the thiobarbituric acid, peroxide value, free fatty acid and moisture content for twelve (12) weeks. The refractive index, specific gravity, acid value and free fatty acid value ranged between 1.46 ̵ 1.47, 0.89 ̵ 0.90, 0.4 ̵ 1.40mg/g, and 0.20 ̵ 0.75% respectively. The peroxide, saponification and iodine values ranged between 3.99 ̵ 4.43meq/kg, 183.25 ̵ 202.87mg/kOH/g and 29.69 ̵ 31.74g/l2/g respectively. The major fatty acids (FAs) of the tiger nut oil were oleic (77.71%), palmitic (16.17%), and stearic (5.08%) acids for the black cultivar, oleic (64.12%), palmitic (11.86%), linoleic (11.87%) and dihum ̵ g ̵ linolenic (1.71%) for the brown cultivar while the yellow cultivar had oleic (68.89%), linoleic (12.77%), palmitic (13.33%) and stearic (4.46%). During storage peroxide value, free fatty acid, moisture content and thiobarbituric acid of the oil were within the maximum limits as recommended by CODEX Alimentarius.

Vegetable oil is one of the principal sources of minor bioactive substances such as tocopherol, phytosterol, and squalene. In order to obtain the distribution of these substances in vegetable oil, this study developed and validated high performance liquid chromatography-tandem mass spectrometry (HPLC-MS) method for the simultaneous analysis of tocopherols (α-, β-, γ-, and δ-tocopherol), phytosterols (β-sitosterol, stigmasterol, brassicasterol, and campesterol), and squalene in vegetable oils. An automatic gel permeation chromatography (GPC) system was used to remove triglycerides from oils, avoiding the heat treatment of traditional saponification. Method validation revealed the accuracy and reliability of this method. The current approach achieved the successful separation of β- and γ-tocopherol, which solved the problem of coelution and inaccurate quantification for β- and γ-tocopherol in previous study. Additionally, this method was applied to detect tocopherols, phytosterols, and squalene in 9 categories of vegetable oils.

A new consumer profile for pharmaceutical and cosmetic products has motivated research into natural raw materials in the development of “green” products such as herbal medicines and biocosmetics. However, various limitations have been encountered in the marketing of these products, for example the quality control of the natural raw materials used by the industrial market. This study aims to evaluate the sensory and physicochemical parameters of murumuru (Astrocaryum murumuru Mart.), bacuri (Platonia insignis Mart.), tucuma (Astrocaryum vulgare Mart.), and ucuuba (Virola sebifera Aubl.) butters for applications in pharmaceutical and cosmetic bioproducts. The acidity and saponification as well as the iodine and peroxide indexes were evaluated and fatty acid profiles for the samples obtained by GC-MS. The sensory properties of the butters showed the appearance of solid to soft cream, color (yellow, brown, buttercup, and ochre), and characteristic odor. The melting temperatures of all butters ranged between 31 oC and 49 °C. The acidity, saponification, iodine and peroxide indexes for the butters were of 5.82 – 17.73 mg (NaOH or KOH) g−1, 181.10 – 573.55 mg KOH g−1, 2.78 – 44.96 gl2 100 g−1, and 1.39 – 9.30 meq kg−1, respectively. From analyses of the fatty acid profiles, the major components identified were lauric acid in murumuru (40%) and ucuuba butters (73%), myristic acid in tucuma butter (53%), and palmitic acid in bacuri butter (42%). In general, the results of the analyses differed from the specifications of the supplier reports and official compendia. These findings highlight the importance of quality control in natural raw materials to ensure their functionality in pharmaceutical and cosmetic bioproducts.

Polyhydroxyalkanoates (PHAs) have attracted attention as an environmentally degradable bioplastic which potentially replaces synthetic polymers used in a wide range of industries. One of most promising microorganisms for the production of PHAs is Pseudomonas putida. In this study, we purpose to develop sustainable processes to convert abundant palm oil available in local market to high value PHAs and optimize PHAs production by Pseudomonas putida TISTR 1522 from saponified palm oil. We found that the highest yield of PHAs production (0.95 g/L, 40.15%) was obtained in culture medium supplemented with 1% (w/v) fatty acid salt by P. putida TISTR 1522 after 24-h cultivation. The intracellular PHAs were located in granules inside the cells, which fluoresced bright yellow by staining with Nile red. The physical appearance of intracellular PHAs investigated by transmission electron microscope (TEM) revealed that PHAs accumulate in granules, about 3–10 granules per cell. These granules are white and roundish-shaped with 0.3–0.5-μm diameter. The 1H NMR spectrum represented the typical characters of medium-chain length-PHAs. This variation of all parameters was successfully demonstrated a good intracellular PHAs accumulation in P. putida TISTR 1522 by fatty acid salt utilization.

Red palm olein (RPOl) is one of the derivatives of palm oil. It contains a high composition of unsaturated fatty acids such as oleic and linoleic, whereas palm kernel oil (PKO) contains more saturated fatty acids of lauric acid. RPOl provides high nutrient contents such as squalene, Vitamin E, and carotene, whereas PKO that is rich in lauric acid can fight Gram-positive microorganisms. This research aims to study the chemical characteristics of RPOl, PKO, and the combination. A combination of RPOl with four different concentrations of PKO (20%, 50%, 80%, and 100%) was analyzed to obtain the composition of fatty acids, squalene content, Vitamin E levels, total carotene, and saponification numbers. RPOL contains high levels of squalene, Vitamin E, and total carotene, followed by RPOl and PKO combination of oil, with a higher percentage of RPOl in its composition. The increase of the PKO level added to the combination will decrease the saponification number and increasing the acid number. Therefore, it can be concluded that RPOl could be the source of squalene, Vitamin E, carotenoids, and oleic acid, whereas PKO is the largest source of lauric acid.

Increasing world population has markedly increased the demand for vegetable oils for domestic and industrial purposes. Plant-based vegetable oils have been identified as one of the oils with high nutritive value. Castor plant is one of the oilseed with rich oil content owing to its high monounsaturated fatty acid and bioactive compounds. Its fatty acid profile constitutes mainly of ricinoleic acid and other minor acids such as stearic, palmitic, and oleic acid. Ricinoleic acid of castor oil is unique among all other vegetable oils, making it attractive for a wide spectrum of applications. The predominant triglyceride component in the oil is triricinolein. Minor biological compounds including carotenoid, tocopherol, tocotrienol, phytosterol, phospholipid, phytochemical, and phenolic compounds are present in castor oil. These compounds offer oxidation stability, anti-inflammatory, and antioxidant properties to the oil. The acid, anisidine, iodine, viscosity, and saponification values indicate that castor has good oil quality compared to other vegetable oils. Castor oil composition is influenced by the area of production and method of extraction adopted. The chemical structure of castor oil is centered on the ricinoleic acid and three major functional groups linked by glycerol moiety. More research on the oil’s component is being investigated nevertheless efficient and eco-friendly extraction methods are required. This review, therefore, summarizes the castor oil composition namely the triglyceride, various fatty acids and bioactive compounds, extraction methods, as well as its physicochemical properties.

A biochemical analysis of Brachystegia aurycoma harm seeds was conducted. Brachystegia, of the family Leguminosae sub-family Caesalpinioidae is a timber tree whose seeds “achi”, are relished as soup condiment by the lgbo-speaking people of Nigeria. The seed were subjected to oil extraction using Soxhlet method. The oil was subjected to chemical analysis to determine the saponification, iodine, free fatly acid and acid values. Proximate analysis was carried out on the seed meal and the seed coat. Results showed that the oil content was 3.33% which is of good quality and fit for human consumtion. The saponification value was 287.85mg/KOH/g, free fatty acid value 6.73mg/KOH/g and iodine value 132.08%. The proximate analysis of the seed meal showed that the moisture content was 15.77%, ash 25%, protein 14.25%, crude fibre 19.05% and nitrogen free extractives (carbohydrates) 21.58%. It shows the seed meal as good source of plant protein and energy and of high roughage and mineral content. The proximate analysis of the seed coal showed that the ash content was 8.33%. The calcium and magnesium contents were 30.00 and 18.00% respectively. This shows that the seed coat can be a supplement in livestock feed. Keywords: Brachystegia, seeds, chemical analysis

The COVID-19 outbreak has overly emphasised the importance of handwashing with soap to reduce the spread of the virus. This study was conducted to formulate a liquid hand soap with neem oil and lemongrass essential oil as an natural antibacterial component. Three different concentrations of neem oil 5%; 10% and 15% and three different concentration of lemongrass essential oil (0.2%; 0.4%;0.6%) were formulated as liquid soap using coconut oil, castor oil and neem oil as its soap bases. The natural liquid soap was made by saponification reaction between oils and potassium hydroxide. The soap was evaluated for its pH value, density, foam stability, insoluble in alcohol content, free fatty acid and antibacterial activity. The results showed that the colour of the liquid soap was transparent with yellowish colored and had distinctive smell of neem oil. The pH values of the different formulated liquid hand soaps are within the accepted pH range of 4 - 10. The specific gravity was 1,083 – 1,088 g/ml. The foam stability range was 25.35% - 78.38%, respectively. The insoluble in alcohol content range were 0.14 – 0.4. The free caustic alkali range was 0.12 – 0.47. The liquid hand soap can inhibit the growth of Staphylococcus aureus. The inhibition zone diameter of liquid hand soap was 1.98- 2.61 cm. It was therefore proven that neem oil and lemongrass essensial oil is effective as an antibacterial component in the formulation of liquid hand soap.

Bee pollen is known for its nutritional value, and therefore it is used in the treatment of some health disorders as food supplements. Herein, the chemical fatty acid profile and mineral contents of two Algerian bee pollen collected from different regions were investigated, along with their physicochemical properties such as specific gravity, refractive index, and acid, saponification, and iodine values. In addition, their total phenolic contents (TPC) were also investigated. Gas chromatography/mass spectrometry (GC/MS) analysis revealed that the bee pollens mainly contained palmitic (17.0 and 26.5 %), oleic (8.5 and 10.1 %), linoleic (15.7 and 12.6 %) and linolenic acids (27.2 and 26.3 %), respectively. The saponification values were 178.54 and 175.73 mg KOH.g-1, while the specific gravity 0.915 and 0.924, the refractive index 1.465 and 1.464, and acid values 22.04, and 10.01 mg KOH.g-1 oil. The iodine values were, however, 44.42 and 32.90 mg I2.g-1 fat, respectively. Potassium and sodium were the main detected elements in both pollen samples with variable percentages. In the DPPH assay, the IC50 was 4.88 and 1.73 mg.mL-1 for samples 1 and 2, respectively, ABTS and phosphomolybdenum assays supported DPPH assay results. It was concluded that the fat part of bee pollen by honeybees (Apis mellifera L.) is a promising source of naturally occurring antioxidants and nutrients.

Following biomass fractionation of corn stover using γ-valerolactone (GVL) pretreatment, the resulting lignin stream was subjected to base hydrolysis reactions to achieve the isolation of p-coumari...

Despite widespread and its local available as a naturalized hedge and shade plant, the potential of Trichilia emetica was not utilized in soap making by the majority of local community in various parts of Dodoma, Tanzania. This study aimed to assess the quantity (yields) and quality (Acid Values (AVs), %Free Fatty Acids (%FFAs) and Saponification Values (SVs) of seed oil from water plant (T. emetica), suitable for soap making application. Solvent extraction method was used during oil extraction, where by 50gm of preheated and powdered seed materials were immersed in 250 ml of n-hexane in 1:5 (w/v) to dissolve the oil contained in the seed cake. The oil was collected by vaporizing solvent out through Rotary evaporation at 60 °C. Also standard titration methods were used to obtain SVs, AVs and %FFAs of the extracted oil. Results showed that T. emetica seeds contained higher quantity of oil (48.4%−50.2%) than many reported commercial plant seed oils. Also, the study found higher AV (7.4 mgKOH/g−7.8 mgKOH/g), %FFA (3.7% to 3.9%) and SVs (189.5 mgKOH/g − 191.4 mgKOH/g) than the maximum acceptable limits of 0.50 mg KOH/g, 0.020% and 175 mgKOH/g − 187 mgKOH/g prescribed by ASTM standards (2002). The obtained results showed that, T. emetica seeds yielded high oil quantity with low qualities due to higher levels of acidity. But high SVs guarantees the possibility of using T. emetica seed oil in soap making. However, the oil requires purification in order to bring levels of acidity to acceptable standards and guarantee its normal use in soap making.

This study investigates the physico-chemical properties of Tithonia diversifolia seed and oil using standard analytical methods and then compared the results with Sunflower oil. The results showed that Tithonia diversifolia seed contained 5.80% moisture, 18.83% crude protein, 30.40% crude fat, 17.85% crude fibre, 4.30% ash and 22.82% carbohydrate. The content of magnesium, copper, iron, zinc and calcium were 3930, 168, 277, 2091 and 432 mg/kg, respectively. Iodine value for T. diversifolia and Sunflower oils, respectively were 109.00 and 145.67 g iodine/kg; saponification values, 212.61 and 188.63 mg KOH/g; ester values, 184.15 and 206.86 mg KOH/g; peroxide values 4.0 and 5.87 meq peroxide/kg; acid values, 5.76 and 4.48 mg KOH / g; % unsaponifiable matter, 0.83 and 1.22 %; and total phenol content, 118.63 and 108.75 µg/g. Others include, specific gravity, 0.937 and 0.920; surface tension 0.042 and 0.051 N/m; viscosity 42.50 and 30.50 cSt; and smoke point, 215 and 245 0C. The study revealed that Tithonia diversifolia seed oil content and physicochemical parameters are comparable with those of Sunflower oil. Hence the oil could be used as raw materials for industrial processes, biodiesel production and a good source of dietary antioxidant which could complement or replace some conventional oils.

Ultrasound-assisted extract of Acalypha indica was used to prepare the green soap formulation. Previously, D-optimal mixture design was used to optimize the mixture of oil components including coconut oil (A, 34–56%), soybean oil (B, 19–31%), castor oil (C, 14–23%), sunflower oil (D, 4–6%), and olive oil (E, 5–9%). Linear regression models were proposed to predict the responses, i.e., hardness (Y1), iodine (Y2), and iodine number saponification (INS) (Y3), and validated with a high degree of statistical accuracy (Fcal > Ftab; df = 4, p 0.9950). Optimization results revealed that the formulation containing 44.57% A, 23.62% B, 17.44% C, 5.37% D, and 9.0% E would yield 41 Y1, 62 Y2, and 159 Y3. The chemical properties of the optimized soap formulation were quite comparable concerning the standard soap specifications (IS13498). Further, this formulation was supplemented with Acalypha indica extract to prepare the green soap, and its antifungal activity was determined using the agar dilution method.

Soaps are the sodium salts or potassium salts of stearic acids. They are prepared by the saponification process, which is, reacting the oil which contains triglycerides with Caustic soda (NaOH) to give the soap. In the present work prepared total ten numbers of herbal soaps by using Guava and Neem leaves extract with 5 different types of oils (Coconut, Teel, Olive, Mustard and Sunflower). In this process used turmeric, indigo, milk powder, henna and coffee powder as a colouring agent and Lemon grass leaves extract as a flavouring agent. After preparation of soaps analyzed different properties of soap such as foaming capacity, yield percentage, lathering power, cleansing power, hardness and moisture content of samples. After analysis conclude that the soap prepared with Coconut oil is best among them and soap with Sunflower oil is poor quality. It has been also concluded that the soap prepared by guava leaf extract can be used for washing wounds.due to anti bacterial properties.

Deodorizer distillates are waste by-product from vegetable oil industries. It has excellent potential as a source of bioactive compounds. Therefore, the aim of this study was to model the thermodynamic phase equilibrium and optimize the supercritical CO2 extraction processes for concentrating valuable minor components from rapeseed oil deodorizer distillates (RODD). The extraction was done in a countercurrent extraction column at pressure (35−40 MPa), temperature (313 K) and solvent to feed mass ratio (S/F) of 5. The raw material employed was initially saponified. Investigating the extraction process, representing phase equilibria behavior of the multicomponent oil-type material/CO2 mixture and prediction the steady-state efficiency of the extractor was done by using the Stryjek–Vera Peng–Robinson equation of state as a thermodynamic model for the experimental bubble pressures and vapor compositions. The extraction of β-sitosterol and vitamin E in the saponified RODD at predicted conditions showed a recovery of 76 wt% with a purity of 60 wt% and a recovery of 85 wt% with a purity of 75 wt% respectively. Furthermore, the use of a modified sample increased the phytosterol and tocopherol efficiency and purity. The absolute average relative deviation between the theoretical and experimental was within the range of 1–12%, which is considered as a good agreement. The coupling of thermodynamic modeling with experimental work offered an efficient and rapid tool for analyzing the viability of the supercritical extraction.

This study aimed to characterize the chemical, antioxidant and antimicrobial properties of the pulp oil of Mauritia flexuosa L. f. (Arecaceae). Chemical identification was performed by gas chromatography coupled to mass spectrometry. The physicochemical properties were characterized. Antioxidant capacity has been verified by eliminating of free radicals, reducing and chelation of iron. The antimicrobial activity was evaluated by the minimum inhibitory concentration and the modulatory action of antibiotics. The major fatty acids identified were stearic acid, palmitic acid and oleic acid. The acidity and the saponification index are within the limits established by the National Sanitary Surveillance Agency. The oil showed moderate antioxidant activity and antimicrobial activity against Candida strains. It also showed synergistic effects, especially on cefotaxime against Bacillus cereus. The results suggest the potential of the species as an antioxidant and in antimicrobial therapy.

Palm kernel oil contains fatty acids, especially lauric acid at 46-52%. Lauric acid has the properties of hardening, cleaning, producing foam and softening that is needed in making soap. The purpose of this study is to make transparent solid soap from a combination of glycerin, sucrose, and ethanol 96% as a transparent agent that meets the requirements of quality tests and organoleptic tests. Made 9 (nine) formulas with some combination of glycerin, sucrose, ethanol 96% by a semi-boiled method that is mixing palm kernel oil and stearic acid which has been melted at 60˚C, then adding 30% NaOH, stirring at 70- 80˚C. Transparent agent and other additives are mixed into the soap mass obtained from the saponification process, then poured into a soap mold and carried out a curing process for 2 weeks. The result of this research is obtained a transparent yellow solid soap that is the bright and distinctive smell of coconut perfume with a pH of 8.35-8.64, the hardness of 21.30-38.13 10-1 mm, moisture content of 21.48-33.33%, free fatty acid content 0,88-0,96%, unsaturated fat content 1,45-2,38%, surface tension 27,93-32,34 dyne/cm. Transparent solid soap that meets the quality requirements and the organoleptic test is formula A with PH 8.64, hardness 25.80 10-1 mm, moisture content 25.55%, free fatty acids 0,94%, unsaturated fat 1,45%, surface tension 30.02 dyne/cm.

In this current study, the economical and environmentally friendly method of extraction and concentration of polyunsaturated fatty acid (PUFA) from Nile tilapia waste was improved. First, Nile tilapia waste was collected and characterized. Lyophilized Nile tilapia had a 30.3% lipid content, which was suitable for oil extraction and PUFA concentration. After extraction and concentration, Nile tilapia waste oil (TWO) concentrated with Pseudomonas fluorescens lipase gave an acceptable peroxide and acid value of fish oils for human consumption. Fatty acid composition was also evaluated. After enzymatic concentration, PUFA increased dramatically. A high percentage of PUFA (59.0%) and the ratio of fatty acids n-6/n-3 (1.2) indicates good nutritional values of enzymatically concentrated TWO. Moreover, the optimization study of enzymatic concentration was evaluated to increase quality of TWO. A concentration of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) was determined. A high concentration of EPA+DHA was obtained when P. fluorescens (3.6%) and Thermomyces lanuginosa (5.3%) lipases were applied. The highest EPA and DHA content (6.0%) was observed with 0.1 g of immobilized T. lanuginosa lipase per 2 g oil after an 18-h reaction time. Similar conditions were also preferred when using P. fluorescens lipase; however, only 4.0% EPA and DHA were observed. This is the first report on PUFA concentration from Nile tilapia waste using a one-step extraction and saponification in combination with enzymatic concentration. Notably, this method is expected to be a more economically possible technique for large-scale production and allows the possibility of using Nile tilapia waste as substrate for PUFA concentration.

Traditional coconut oil is coconut oil that is produced for generations in a simple way. This coconut oil cannot last long without purifying. This oil is produced by heating so that the quality of the oil decreases. Improving the quality of thisoil can be done by adding extract of ethanol pandanus fragrant which is easily obtained and contains natural antioxidants. This study aims to determine the characteristics of traditional coconut oil after the addition of ethanolic extract of pandanus fragrant leaves. The study was conducted following a randomized block design (RBD) of one factor, namely the concentration of ethanol extract of pandanus leaves consisting of 6 levels of treatment, namely p 1 = 0,00%, p 2 = 0.02%, p 3 = 0.04%, p 4 = 0.06%, p 5 = 0.08%, and p 6 = 0.10% , with 4 replications. Data were analyzed by ANOVA test and further tests using BNJ test with a level of 5%. The results showed that the best treatment was obtained at the ethanol extract concentration of 0.10% pandanus fragrant leaves that produce coconut oil with a water content of 0.128%, free fatty acid content of 0.489%, and a saponification rate of 229.22 mg KOH/g of oil. The highest descriptive sensory characteristics of traditional coconut oil are yellowish green (4.48) and slightly pandanus (3.20)

Natural shea butter (NSB), extracted by traditional methods resulting in its poor quality, is nevertheless widely traded within Africa and beyond due to its several useful applications. This study examined effects of simulated laboratory/domestic heat treatments on quality of the commodity obtained from a cross section of Nigerian markets. Physicochemical and microbiological qualities of NSB samples procured from four selected markets located across three Nigerian states were evaluated by standard methods before and after graduated thermal stress treatments from 50 through 120 °C over 5, 15, or 30 min durations, respectively, and filtration at 60 °C. Mean physicochemical quality values of NSB samples determined, namely: specific gravity at 29 °C (0.90-0.94); softening, melting temperatures (33-36, 36-39 °C; respectively); acid, iodine, and saponification values (10.5- 29.3, 46.4-59.1, 110-470; respectively), were not adversely or significantly altered by the thermal treatment types and stresses. Whereas all untreated NSB samples demonstrated microbial contamination (total viable counts: 3 6 10 -10 cfu/g) with Pseudomonas, Klebsiella, Staphylococcus, Bacillus, Aspergillus, or Candida species, the graduated heat treatments produced varied sanitizing effects. Higher temperatures (100, 120 °C) gave greater and more rapid cleansing action than the lower temperatures (50, 75 °C), both intensity-ranges being aided by length of holding time. Hot filtration eliminated all the NSB contaminants. In conclusion, while untreated NSB is found grossly contaminated by microbes, unhygienic and unsafe for human use, this study has demonstrated efficient contaminants-cleansing action of heat treatments (³100 °C × ³ 30 min) on NSB, and the total sanitizing effect of hot filtration process. Key words: Natural shea butter, Physicochemical quality, Microbiological quality, Heat treatment, Hot filtration.

Minyak kelapa murni merupakan bahan makanan yang banyak digunakan sebagai bahan konsumsi dan bahan tambahan untuk proses industri. Minyak kelapa murni diproduksi dari bahan baku krim santan yang merupakan hasil pelindihan antara air dengan buah kelapa. Pemecahan emulsi pada santan menggunakan teknik ekstraksi kering (dry extraction) dan teknik ekstraksi basah (wet extraction). Penelitian yang dilakukan menggunakan proses ekstraksi basah, yaitu secara fermentasi menggunakan inokulum padat berupa mikroba atau ragi yang dikembangkan pada media padat. Proses tersebut dipilih untuk menyesuaikan dengan situasi masyarakat daerah di Indonesia yang banyak menghasilkan kelapa serta menyesuaikan dengan keberadaan ragi tempe yang telah memasyarakat. Tujuan penelitian ini adalah untuk mendapatkan minyak kelapa dari bahan baku santan (coconut milk) dengan menggunakan inokulum ragi tempe yang mengandung Rhizopus oligosporus. Hasil minyak kelapa murni akan dianalisa parameternya berdasarkan Standar Nasional Indonesia. Dari data percobaan di peroleh hasil analisa produk minyak kelapa murni yang paling optimum sesuai dengan SNI adalah percobaan pada variasi jumlah ragi tempe 0.5 gram dengan jumlah minyak yang dihasilkan 91.35 gram dan nilai kekeruhan 1,8 NTU. Hasil analisis kimia dan fisik: Indeks bias: 1,451, Kadar air: 0,3%, Kadar kotoran: 0,023%, Kadar bebas asam lemak: 0,96%, Kadar minyak Pelikan: 1,82%, angka saponifikasi: 262,969 mgKOH / g lemak. Virgin coconut oil is a food ingredient that is widely used as a consumption material and as an additive for industrial processes. The oil produced from coconut milk cream which was the result of leaching between water and coconut. The emulsion in coconut milk then broken by dry extraction method and wet extraction method. In this research the method carried out by a wet extraction process, which used fermentation using microbes or yeast that was developed on solid media. This process was chosen to adapt to the Indonesian people who produced ton coconut each year and in the same time, the tempeh yeast that has been popular in Indonesia. The purpose of this study was to obtain virgin coconut oil from coconut milk using yeast inoculum containing Rhizopus oligosporus. The parameters of virgin coconut oil analyzed based on the Indonesian National Standard (SNI). The experimental data obtained from the results of the most optimal analysis of virgin coconut oil in accordance with SNI experiments on the variation of the amount of tempeh yeast 0.5 grams with the amount of oil produced 91.35 grams and turbidity value 1.8 NTU. Chemical and physical analysis results: Refractive index: 1,451, Air content: 0,3%, Dirt content: 0,023%, Free fatty acid level: 0,96%, Pelicans oil content: 1,82%, saponification rate: 262,969 mg KOH /g fat.

Aims: The study was aim at extraction, saponification and quality determinations of shea nut fat samples obtained from different major sheanut producing areas of Kebbi state. Study Design: The quality determination done with and without additives such as perfume, colour and silicate in terms of pH, foam stability and height from four major shea nut producing areas in Kebbi state, Nigeria. Methodology: The dry powdered sample of ground shea kernel was extracted in soxhlet apparatus using n- hexane as the extracting solvent. Cold process saponification was used in preparing the soap. The soap pH was measured using pH metre. Foam ability of the soaps was also determined. Results: The highest foam height was observed on the shea soap from Tsamiya (60 cm3), Kwanga (50 cm3), Kwere (40 cm3) with colour additive and the lowest on soap from Illo with perfume additive. For the foam stability test, shea soap from Tsamiya at 43 cm3 was the highest followed by Kwanga at 38 cm3 both with colour additive. Lowest foam stability was recorded on shea soap from Illo with perfume additive. The pH of the shea soap from Illo without additive (9.73) increased with addition of colour, silicate and perfume at 10.70, 10.98 and 11.63 respectively. It showed decrease on addition of perfume, silicate and colour from 11.72 to 10.45, 11.38 and 10.15 respectively from Kwanga. Conclusion: Variation occurs in the foam stability and pH of the shea soap from the four locations and the addition of colour, perfume and silicate had effect on the foam stability and pH of the shea soaps.

Palm fruit as raw material of palm oil is the main commodity of Indonesian agriculture. Palm fatty acid distillate (PFAD) is a by-product of palm oil refinery process in deodorization stage. PFAD contained bioactive compounds such as vitamin E, phytosterols, and squalene. Vitamin E, phytosterols, and squalene have knows for its hypocholesterolemic effect and cardio protector. Bioactive compounds of PFAD can be separated from its fatty acid using saponification reaction. Saponification of PFAD produced unsaponifiable matter (USM) which was rich in bioactive compounds. Food fortification is aimed to enhance the nutrition quality of food for specific reason. The fortificant can be macronutrient or micronutrient. USM of PFAD potentially become food product fortificant which will give hypocholesterolemic effect. This review is discussed about hypocholesterolemic effect of each bioactive compounds in USM of PFAD and the potential of USM as food fortificant espsecially for food with hypocholesterolemic effect.

The purpose of this article is to compare the quality of three edible oils derived from palm oil in Daloa’s city. The methodological approach of the article includes, on the one hand, a field survey determining the most used oils and the number of frying carried out by the attieke-fish sellers. On the other hand, the physico-chemical parameters (refractive index, iodine index, saponification index, acid index and peroxide index) of these oils were determined. As a result, the most used oils are A, B and C and are heated at least 3 times by the attieke-fish sellers. After repeated heating, the values of the peroxide, saponification, refraction, iodine indices of oils A and B do not match those of the codex with the exception of the acid value. For oil C, only the acid and iodine indices correspond to the codex standards. Overall, the results of the study clearly show that the repeated heating of these oils has an influence on the different physico-chemical properties studied.

Squalene is a precursor of steroids with diverse bioactivities. Tea was previously found to contain squalene, but its variation between tea cultivars remains unknown. In this study, tea leaf squalene sample preparation was optimized and the squalene variation among 30 tea cultivars was investigated. It shows that squalene in the unsaponified tea leaf extracts was well separated on gas chromatography profile. Saponification led to a partial loss of squalene in tea leaf extract and so it is not an essential step for preparing squalene samples from tea leaves. The tea leaf squalene content increased with the maturity of tea leaf and the old leaves grown in the previous year had the highest level of squalene among the tested samples. The squalene levels in the old leaves of the 30 tested cultivars differentiated greatly, ranging from 0.289 to 3.682 mg/g, in which cultivar “Pingyun” had the highest level of squalene. The old tea leaves and pruned littering, which are not used in tea production, are an alternative source for natural squalene extraction.

In this study, tailored-made citrus pectin-derived compounds were produced through controlled enzymatic and/or chemical modifications of commercial citrus pectin with different degrees of methylesterification (DM) and similar average molecular weight (MW). In the first treatment, degradation of the citrus pectin (CP) materials by endo-polygalacturonase (EPG) yielded pectins with average Mw's (between 2 and 60 kDa). Separation and identification of the oligosaccharide fraction present in these samples, revealed the presence of non-methylesterified galacturonic acid oligomers with degree of polymerization (DP) 1-5. In the second treatment, exploiting the combined effect of EPG and pectin lyase, compounds with MW between 2 and 21 kDa, containing methylesterified and non-methylesterified polygalacturonans (DP 1-6), were generated. Finally, CP was sequentially modified by chemical saponification and the action of EPG. A sample of DM 11% and MW 2.7 kDa, containing POS (DP 1-5), was produced. Diverse pectin-derived compounds were successfully generated for further studies exploring their functionality. ispartof: FOOD CHEMISTRY vol:367 ispartof: location:England status: published

The study reports the alternative use of non-edible fermented olives for the production of high-quality natural soaps with a fast production process, low environmental impact, and without preliminary treatments for the raw material. Damaged olives, not used as food, were fermented naturally and the oil was extracted by mechanical extraction. The product obtained was not for human consumption due to its high acidity, but it had a low content of peroxides. The non-edible olive oil obtained and an extra virgin olive oil, produced from the same olive cultivar, were subjected to saponification with sodium hydroxide. The soaps were produced with complete (0% of non-neutralized fatty acids) and incomplete (5% of non-neutralized fatty acids) saponification, the amount of sodium hydroxide to be used was determined with the saponification index. The soaps were aged for six months by monitoring pH, color, and behavior in an aqueous solution. The results show that the olives’ fermentation improves and speeds up the soap production and maturation process since the oil obtained from fermented non-edible olives is more suitable for the saponification process than the oil obtained from non-fermented edible olives. Non-edible fermented olives can be used for obtaining natural and high-quality soaps, reusing drupes classified as food waste.

Abstract. In ASEAN countries, small freshwater fish species contribute to the nutritional needs of people with few livelihoods by providing them with significant amounts of protein, fat, vitamins and minerals. Some species are eaten whole (with their organs, skin, bones, head and eyes). To estimate the vitamin A content of these foods, conventional saponification has been applied but has not been able to fully release the retinol. Our objective was to optimise the conditions of vitamin A saponification in whole fish to have a reliable estimate of their contribution to intakes. The effects of temperature and saponification time on the retinol quantification of whole fish were evaluated using a two-factor experimental design. Reaction time had a significant effect on the saponification of standard retinyl palmitate and whole fish (p≤0.05). For whole fish, the best conditions for the saponification were to heat the samples to 80 °C for 43 minutes. Under these conditions, the retinol is well liberated from the matrix and protected from degradation and isomerisation reactions. The time-temperature couple used is more intense than that recommended for quantifying vitamin A in milk or enriched margarines. The protective effect of the food matrix against the release of retinol is evident. Vitamin A2 alcohol (3,4-didehydroretinol) was detected in five species and the overall vitamin A contents ranged from 9.6 to 737.5 μg RE/100 g in species frequently consumed in Cambodia. The two species of small fish consumed whole were the ones that contained significantly more vitamin A among the ten tested (p≤0.05). Highlights: Vitamin A2 alcohol was quantified in five fish species. The official saponification partially released retinol in whole fish. The optimised reaction required heating the sample to 80 °C for 43 min.

This study aims to assess for the first time the content of phytosterols (PS) in espresso coffee (EC) to deepen the knowledge about the phytochemicals and health potentials of coffee brews. PS were extracted by hot saponification from 14 EC samples produced with coffee originating from 13 coffee-producing countries. PS were identified and quantified by high-performance liquid chromatography (HPLC) after derivatization. Among the detected PS, β-sitosterol (4.1–18.2 mg/L) was the most abundant followed by stigmasterol (1.1–4.9 mg/L), campesterol (0.9–4.7 mg/L), and cycloartenol (0.3–2.0 mg/L). Total PS fraction ranged from 6.5 mg/L to 30.0 mg/L with an average level of 15.7 ± 5.8 mg/L. Therefore, a standard cup of EC (25 mL) could provide 0.4 ± 0.1 mg of PS.

Polycyclic aromatic hydrocarbons (PAHs) were analyzed using gas chromatography–mass spectrometry in 115 dairy products and beverages, including alcoholic, grain, carbonated, and functional drinks; fruit and vegetable juices; coffee; and tea, purchased from 10 local city markets in South Korea. The sample groups were divided into non-fatty and fatty groups, pretreated with the ultrasound-assisted extraction method and saponification method, respectively. The limit of detection, limit of quantification, and accuracy were 0.038–0.185 μg/kg, 0.114–0.560 μg/kg, and 87.64–112.25%, respectively. The measurement uncertainty was ≤ 6.38% for eight PAHs (PAH8). PAH8 was detected in 41 of the 115 samples, ranging from 0.041 to 7.793 µg/kg. The risk assessment revealed that the margin of exposure for PAH8 ranged from 3.60 × 10(4) to 7.84 × 10(11) in the mean intake groups and from 3.60 × 10(4) to 5.33 × 10(11) in the P97.5 intake groups. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s10068-021-00927-7.

Synthetic antimicrobial agents, like triclosan, used in many cosmetics are now associated with serious health problems, beginning with skin irritation to cancer. This calls for alternative antimicrobial agents. Many plant extracts have the potential to inhibit the growth of microorganisms and perhaps kill them and thus can serve as alternative antimicrobial agents in cosmetic formulations. To demonstrate this, a hand-washing liquid soap was prepared in the presence of varying concentrations of aloe vera extract and tested in-vitro against common skin microbes. First, a liquid soap paste was prepared by saponifying a vegetable oil blend containing olive oil (80.0 %w/w), coconut oil (14.3 %w/w) and castor oil (5.7 %w/w) with aqueous KOH solution (22.5 %w/w) at a relatively high temperature (200 °C). Second, the soap paste was diluted with distilled water in the presence of varying concentrations (0 to 66.67 %w/w) of aloe vera extract to obtain hand-washing liquid soap samples, with the extract acting as an antimicrobial agent. Third, the liquid soap solutions were characterised in terms of foaming, wetting and cleansing abilities and the ability to inhibit the growth of Staphylococcus aureus, Pseudomonas aeruginosa and the fungus Aspergillus flavus. These were compared with those of a commercial (Astonish) hand-washing liquid soap sample containing triclosan (0.1 %w/w) as an antimicrobial agent. The liquid soap solutions exhibited excellent foaming, wetting and cleansing abilities, similar to the commercial liquid soap solution. In addition, the liquid soap solutions gave an average zone of inhibition between 9.0±0.3 mm and 11.0±0.2 mm for S. aureus, 7.0±0.1 mm and 10.0±0.2 mmfor P. aeruginosa and also inhibited the growth of the fungus A. flavus. These results compare favourably ( p= 0.05)with the commercial liquid soap solution, which gave an average inhibition zone of 9.00±1 mmfor both bacteria and also inhibited the growth of the fungus. This indicates that aloe vera extract can be used as an antimicrobial agent in the formulation of antimicrobial hand-washing liquid soap and other related products, rather than synthetic agents which are inherently harmful.

Plants consist of triterpenoids such as phytosterols (PT) (C(29)H(50)O) with steroidal nuclei, including sitosterol, stigmasterol, brassicasterol and campesterol. They are hydrophobic but soluble in alcohol and other organic solvents and are isolated from industrial waste deodorizer distillates of various edible oil industries. They exist as free PT or their ester derivatives in soybean, rice, wheat, oat, cottonseed and corn fiber, and other cereals and grains. Conventional isolation techniques such as solvent extraction, distillation, evaporative fractionation, saponification and chemical esterification are employed for isolation and purification of PT. The present article reviews the various advanced separation techniques like solvent crystallization, supercritical fluid extraction, high speed counter-current chromatography and enzymatic process as strategic methods to isolate and purify sterols.

Recent studies have shown that heating oil to high temperature causes oxidative degradation of the oil, consequently producing characteristics odour, taste, colour and texture. However, this study was conducted to evaluate the stability of four different samples of edible oils. Acid value was found to be 5.22, 3.50, 0.73 and 1.29 mg KOH/g oil in fresh Melon, Bushmango, olive and Palm olein oils samples respectively. When the four oil samples were used for frying, the acid value was found to be; 6.73, 3.90, 2.24 and 2.24 mg KOH/g oil (24 hours after frying), 11.22, 7.86, 4.49 and 3.3 mg KOH/g oil (2 weeks after frying) and 19.17, 13.22, 5.82 and 4.4 mg KOH/g oil (4 weeks after frying), for melon, Bushmango, olive and palm olein oils samples respectively. Saponification values were ranged between 196.70-240.00 mg KOH/g oil in fresh oils of the samples, and between 197.80-252.33 mg KOH/g oil in the oils after using them for frying. Furthermore, Peroxide values ranged from 2.65 to 3.20 Meq/Kg in fresh oils of the samples, and from 2.71 to 24.00 Meq/Kg in frying oils were to exceed the permitted value of 20 meq/Kg for olive oil and 10 meq/Kg for other edible oils in nearly half the analyzed samples. In general, feeding on these kinds of edible oils which have high values of acidity and peroxides is very dangerous because of the destructive effects of peroxide compounds and acidity on the components of the membranes of the living cell.

—The optimum parameters of oil extraction from the processed fruit of mountain ash (Sorbus aucuparia) were determined using the second-order rotable composite design: pressure 321 atm, temperature 85°С, and extraction time 72 min. The predicted yield of oil expected under these conditions (9.06%) was confirmed experimentally (9.02%). The antioxidant activity (AOA); fatty acid composition; and acid, iodine, and saponification numbers of the oil extract were determined. For comparison, the oil obtained by extraction with n-hexane was used.

A Gram-stain-negative, non-spore-forming, non-motile, short-rod-shaped and aerobic bacterial strain (designated lm1T) was isolated from propylene oxide saponification wastewater activated sludge obtained from a wastewater treatment facility in Binzhou (Shandong province, PR China). Strain lm1T grew between 15 and 45 °C (optimum, 40 °C). The pH range for growth was at pH 4.0–10.0 (optimum growth at pH 8.0). The range of NaCl concentration for the growth of strain lm1T was 0–4.0 % (w/v), with optimum growth at 1.0–2.0 % (w/v). Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain lm1T belonged to the genus Rhodoligotrophos and was closely related to Rhodoligotrophos appendicifer JCM 16873T (96.7 % 16S rRNA gene sequence similarity) and Rhodoligotrophos jinshengii CCTCC AB2013083T (96.2 %). The average nucleotide identity value and the digital DNA–DNA hybridization value between strain lm1T and R. appendicifer JCM 16873T were 73.4 and 14.3 %, respectively. The major cellular fatty acids of strain lm1T were C19 : 0cyclo ω8c, C18 : 1ω7c and C16 : 0. Strain lm1T contained Q-10 as the predominant respiratory quinone. The polar lipid profile was composed of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, diphosphatidylglycerol, three unidentified aminolipids, one unidentified glycolipid and five unknown lipids. The G+C content of the genomic DNA was 64.4 mol%. Based on polyphasic taxonomic data, strain lm1T could be classified as a representative of a novel species of the genus Rhodoligotrophos , for which the name Rhodoligotrophos defluvii sp. nov. is proposed. The type strain is lm1T (=CCTCC AB2019071T=KCTC 72156T).

A very good understanding of the structure, level, type and physical property relationships of fatty acids in plants oils and their methyl esters (FAMEs) is of utmost importance when selecting vegetable oils for a particular desired biodiesel quality that meets the operating condition requirements of the compression-ignition diesel engine and the climatic dictate of the environment under which the engine is operated. It is on this premise that the degree of influence of fatty acid configuration, chain length, branching and unsaturation on cold flow and critical properties of biodiesel was investigated. The critical properties studied include: saponification and cetane number, iodine value, higher heating values, density, flash point and kinematic viscosity. The feed stock consists of three groups of vegetable oil biomass. The group one is made up of highly saturated, lauric coconut and palm kernel oils while group two consists of high linoleic, soyabean and corn oil biomass with low percentage of mono-unsaturated and high percentage of poly-unsaturated fatty acid. High oleic olive and canola oil constituted the third group of biomass. The triglycerides in these oils were converted to methyl esters by alkali-catalyzed transesterification reaction under standard conditions. The fatty acid methyl esters (FAMEs) compositional analyses of these feed stocks was done by using Agilent, HP 6890 Gas Chromatograph equipped with Flame ionization detector and 6890 Auto Sampler that connects with a controller box (GC-FID). The various biodiesel cold flow behaviour and critical properties under investigation were evaluated by the American society for testing materials (ASTM D6751-07b) and the European union (EN 14214) standard procedures and techniques.Better cold flow behaviour was exhibited by biomass with higher degree of unsaturation, longer chain length, higher degree of branching and with cis configuration. While critical properties showed a lot of variations based on fatty acid profile. Keywords: Biomass, Branching, Chain length, Cold flow behaviour, Critical properties, Gas chromatography, Transesterification reaction, Unsaturation

The primary sources of carotenoids are plants and microorganisms (yeast, bacteria), though they can be synthesized chemically. Due to their potent antioxidant properties, carotenoids exert a significant role at the molecular level in the suppression of various cancers and other oncological diseases. The present research aimed to optimize the enzyme-assisted extraction of carotenoids from carrot by response surface methodology. The Box-Behnken design was used for the optimization of extraction conditions from fresh carrot. The response (yield) was quantified using a spectrophotometric method. The highest yield from the experimental result was 393.4 µg/mL followed by 335.4 µg/mL whereas 198.0 µg/mL represents the least. The predicted highest yield (407.4 µg/mL) was more than the experimental yield (393.4 µg/mL). The model was significant (p=0.0261). However, the lack of fit for the ANOVA was not significant (p=0.3534).

The palm oil (Elaeis guineensis Jacq.) is widely produced in the Brazilian Amazon region; however, the expansion of its cultivation to other environments is mandatory to attend the increasing demand of the industry, especially in the food and biodiesel fields. This study aimed to analyze the lipid profile and physicochemical characteristics of crude palm oil obtained from different palm genotypes cultivated in Goiana-PE, Northeast of Brazil. All genotypes showed high oil yield (> 60%). Lipid profile showed that the palmitic and linoleic acids were predominant in the oil (about 80%). Iodine and saponification index ranged from 50.2 to 55.3g I2.100 g-1 and 184.3 to 185.4 mg KOH.g-1 of oil, respectively. Saponification and iodine levels also showed similar values among the studied genotypes. Acid and peroxide indexes presented values within the National Health Surveillance Agency (ANVISA). BRS C7201 genotype presented more attractive and viable results for its cultivation and development in the studied area.

Oil extracted from fish waste is considered as a value‐added product. The effect of postmortem processing times (0, 3, 6, and 9 hr) and blanching methods (sodium chloride, pH shift, and high temperature) on the extracted oil from rainbow trout viscera was studied. Blanching was applied six hours prior to oil extraction to counteract the effects of delayed processing time and increasing the oil stability. Autolysis by digestive enzymes is main culprit of higher contents of free fatty acids, lipid oxidation, saponified compound, and saturation degrees in case of postponed oil extraction. Results showed that PV was increased after pH shift and high temperature blanching, while there were no significant differences by using salt blanching. The lowest amount of TBA, AV, Totox, and saponification index was observed in salt blanched treatment. The colorimetric values including L*, b*, and whiteness index were decreased after pH shift, whereas redness was increased. Unfavorable coloration could be attributed to the lipid oxidation process that giving rise nonvolatile decomposition products with carbonyl groups. Our results indicated that salt blanching could reduce the effects of delayed processing time and lead to higher quality value‐added product from rainbow trout viscera.

The objective of this study was to develop a safe and ecologically sustainable protocol, to extract and separate lutein and chlorophyll from spinach discards. A saponification in an aqueous medium was carried out to release the phytyl aliphatic chain from the porphyrin ring of chlorophyll. Polar chlorophyll derivatives were formed, remaining in the aqueous medium, whereas apolar lutein remaining in the plant residues and was extracted thereafter by maceration in 95% ethanol. However, the saponification step may cause a significant degradation of lutein, resulting in low extraction yields. The saponification parameters were studied using response surface methodology to determine the conditions providing the highest recovery of lutein in an ethanolic extract, combined with the lowest loss of chlorophyll derivatives. Through this procedure, using the Derringer’s desirability function, we uncovered that the optimal conditions for saponification were 16 h of aqueous maceration with 3.9% (W/V) sodium hydroxide at 60 °C. This procedure can be easily scaled for high volume commercial extraction of these compounds.