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The quantitative effects of oxygen supply in terms of shaking speed and medium volume on the production of mycelia and extracellular polysaccharide (EPS) using Ganoderma tsugae in submerged fermentation were investigated. Mycelia growth required the proper shaking speed at 134 rpm for breaking a larger pellet into several smaller pellets. Furthermore, high level of medium volume for consumption of abundant nutrient is needed for maximum mycelia growth. For EPS, a high agitation is needed to promote a good mass transfer for achieving high product concentrations recovery. The appropriate medium volume was found to be 150.4 mL for aiding to produce a secondary metabolite by promoting the mass transfer of substrates. The dry cell mass (DCM)-EPS diagram of G. tsugae was created from the both production equations which this diagram is an useful tool for submerge fermentation industry for decision about to control the both productions.

Ganoderma tsugae has long been a well known medicinal mushroom and it has many pharmacological properties. The mathematical relationship of productions from G. tsugae is quite interested from industrial fermentation to predict and control the bioprocess. Therefore, the kinetic models of biomass and extracellular polysaccharide (EPS) by G. tsugae were studied in a batch cultivation at the optimal condition. The pellet of mycelium was described by the cube-root equation whereas the extracellular polysaccharide was explained by Luedekin-Piret equation. The parameters of booth equations determine by observed experiment and algorithm solving. The correlation between the experimental values and predicted models of biomass and EPS for G. tsugae obtained the high R-square at 0.9605 and 0.9916, respectively. The both kinetic models may be useful to predict the both productions of G. tsugae.