16 results on '"Masaharu Ikegaki"'
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2. Dynamic Gastrointestinal Digestion/Intestinal Permeability of Encapsulated and Nonencapsulated Brazilian Red Propolis: Active Compounds Stability and Bioactivity
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Severino Matias de Alencar, Alan Giovanini de Oliveira Sartori, Damla Dag, Pollyanna Souza Batista, Pedro Luiz Rosalen, Masaharu Ikegaki, and Fanbin Kong
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General Medicine ,Food Science ,Analytical Chemistry - Published
- 2022
3. The effect of seasons on Brazilian red propolis and its botanical source: chemical composition and antibacterial activity
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Pedro Luiz Rosalen, Masaharu Ikegaki, Bruno Bueno-Silva, Severino Matias de Alencar, and Alexandre Marsola
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Staphylococcus aureus ,PRÓPOLIS ,Microbial Sensitivity Tests ,Plant Science ,01 natural sciences ,Biochemistry ,Propolis ,Streptococcus sobrinus ,Analytical Chemistry ,Streptococcus mutans ,chemistry.chemical_compound ,Chalcones ,Botany ,Actinomyces ,Formononetin ,Food science ,Chromatography, High Pressure Liquid ,Flavonoids ,biology ,010405 organic chemistry ,Organic Chemistry ,biology.organism_classification ,Antimicrobial ,Isoflavones ,Anti-Bacterial Agents ,0104 chemical sciences ,010404 medicinal & biomolecular chemistry ,chemistry ,Actinomyces naeslundii ,Seasons ,Antibacterial activity ,Brazil ,Isoliquiritigenin - Abstract
The aim of this study was to evaluate the effect of seasons on the chemical composition and antibacterial activity of Brazilian red propolis (BRP) and its plant source. BRP was collected from Maceio, Alagoas state, north-east of Brazil, during one year. Chemical composition was determined by physicochemical analyses and HPLC while antimicrobial activity was assessed against Streptococcus mutans, Streptococcus sobrinus, Staphylococcus aureus and Actinomyces naeslundii by determining the minimal inhibitory and bactericidal concentrations (MIC and MBC, respectively). The comparative chemical profiles varied quantitatively according to the collection period. Formononetin was the most abundant compound in both propolis and resin, while isoliquiritigenin, (3S)-neovestitol, (3S)-vestitol are suggested to be responsible for antimicrobial activity of Brazilian red propolis. MIC varied from 15.6 to 125 μg/mL, whereas MBC varied from 31.2 to 500 μg/mL. Therefore, season in which propolis and its botanical source are collected indeed influences their chemical compositions, resulting in variations in their antibacterial activity.
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- 2016
- Full Text
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4. The correlation between the phenolic composition and biological activities of two varieties of Brazilian propolis (G6 and G12)
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Ingridy Simone Ribeiro Cabral, Severino Matias de Alencar, Pedro Luiz Rosalen, Tatiane Luiza Cadorin Oldoni, and Masaharu Ikegaki
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Antioxidant ,Compostos fenólicos ,medicine.medical_treatment ,Brazilian própolis ,Flavonoid ,lcsh:RS1-441 ,Flanovóides^i2^sdeterminação no própo ,Propolis ,Compostos fenólicos^i2^sdeterminação no própo ,lcsh:Pharmacy and materia medica ,Própolis ,Propolis^i1^squality cont ,medicine ,Bioassay ,Brazilian própolis^i1^sevaluat ,Food science ,General Pharmacology, Toxicology and Pharmaceutics ,Própolis^i2^scontrole de qualid ,Flavonoids ,chemistry.chemical_classification ,Chemistry ,Própolis brasileira^i2^savalia ,fungi ,Biological activity ,Antimicrobial ,Phenolic compounds^i1^sdetermination in propo ,Phenolic compounds ,Flanovóides ,Flavonoids^i1^sdetermination in propo ,Própolis brasileira ,Composition (visual arts) - Abstract
Biological assays that have been performed on different types of Brazilian propolis have shown that type 6 propolis (G6) has a strong antimicrobial activity and a low flavonoid content. This study aimed to evaluate the correlation between the phenolic composition and the biological activities displayed by propolis G6 from the state of Bahia and green propolis, also known as type 12 (G12). The values of the flavonoids and the total phenolics in propolis G6 were different than those in propolis G12. Although the G12 variety presented greater antioxidant activity, propolis G6 proved to have greater antimicrobial and cytotoxic activities. The results showed that the phenolic compounds may not be the only compounds responsible for the biological activity. More detailed studies of the chemical composition and an assessment of the biological activity are required to establish the quality of propolis.Ensaios biológicos realizados com diferentes tipos de própolis brasileira têm mostrado que a própolis do tipo 6 (G6) tem forte atividade antimicrobiana e menor teor de flavonóides. Este trabalho teve como objetivo avaliar a correlação entre a composição fenólica e atividades biológicas apresentadas pela própolis G6, do Estado da Bahia, e a própolis verde, do tipo 12 (G12). Os teores de flavonóides e fenólicos totais na própolis G6 foram diferentes dos teores da própolis G12. Apesar da G12 apresentar maior atividade antioxidante, a própolis G6 apresenta maior atividade antimicrobiana e citotóxica. Os resultados mostraram que os compostos fenólicos não são os únicos compostos responsáveis pela atividade biológica da própolis. Estudos mais específicos da composição química, em adição à avaliação das atividades biológicas, são requeridos para determinar a qualidade da própolis.
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- 2012
5. Própolis do sudeste e nordeste do Brasil: influência da sazonalidade na atividade antibacteriana e composição fenólica
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Pedro Luiz Rosalen, Simone Duarte, Masaharu Ikegaki, Jaime Aparecido Cury, Hyun Koo, Severino Matias de Alencar, and Myrella Lessio Castro
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propolis ,Chemistry ,Botany ,Composition (visual arts) ,phenolic compounds ,General Chemistry ,Food science ,Propolis ,Antimicrobial ,Antibacterial activity ,seasonal effect ,Chemical composition - Abstract
The composition and biological activities of propolis, a resinous hive product collected by honeybees from various plant sources, depends on various factors such as season and vegetation of the area. The aim of this study was to evaluate the influence of the seasonal effect on the ethanolic extracts of Brazilian propolis (EEP) type 6 and type 12, collected during 6 months in terms of antibacterial activity and phenolic composition. The antimicrobial properties were evaluated by MIC and MBC on S. mutans Ingbritt 1600 and the profile of chemical composition by UV-visible spectrophotometry, HPLC-RF and GC-MS. The results demonstrated that the season in which propolis is collected influences its chemical composition, resulting in modifications in its antibacterial activity.
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- 2007
6. Chemical Characterization and Antioxidant, Antimicrobial, and Anti-Inflammatory Activities of South Brazilian Organic Propolis
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Severino Matias de Alencar, Pedro Luiz Rosalen, Carina Denny, Marcelo Franchin, Ana Paula Tiveron, Risia Cristina Coelho Lacerda, Masaharu Ikegaki, Bruno Bueno-Silva, and Bruna Benso
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0301 basic medicine ,Chemical Radicals ,Antioxidant ,medicine.medical_treatment ,PRÓPOLIS ,Anti-Inflammatory Agents ,lcsh:Medicine ,Pathology and Laboratory Medicine ,Biochemistry ,Physical Chemistry ,Antioxidants ,Mice ,White Blood Cells ,0302 clinical medicine ,Animal Cells ,Medicine and Health Sciences ,Food science ,lcsh:Science ,Liquid Chromatography ,chemistry.chemical_classification ,Multidisciplinary ,Antimicrobials ,Chromatographic Techniques ,NF-kappa B ,Drugs ,Pseudomonas Aeruginosa ,Free Radical Scavengers ,Antimicrobial ,Anti-Bacterial Agents ,Bacterial Pathogens ,Chemistry ,Medical Microbiology ,030220 oncology & carcinogenesis ,Physical Sciences ,Pathogens ,Cellular Types ,Research Article ,Free Radicals ,medicine.drug_class ,Immune Cells ,Immunology ,Biology ,Research and Analysis Methods ,Microbiology ,Propolis ,Anti-inflammatory ,03 medical and health sciences ,Minimum inhibitory concentration ,Phenols ,Streptococcal Infections ,Streptococcus Mutans ,Microbial Control ,Pseudomonas ,medicine ,Animals ,Humans ,Pseudomonas Infections ,Microbial Pathogens ,Pharmacology ,Reactive oxygen species ,Blood Cells ,Bacteria ,Tumor Necrosis Factor-alpha ,Macrophages ,lcsh:R ,Organisms ,Chemical Compounds ,Streptococcus ,Biology and Life Sciences ,Cell Biology ,biology.organism_classification ,Streptococcus mutans ,High Performance Liquid Chromatography ,RAW 264.7 Cells ,030104 developmental biology ,Streptococcus oralis ,chemistry ,lcsh:Q - Abstract
South Brazilian organic propolis (OP), which has never been studied before, was assessed and its chemical composition, scavenging potential of reactive oxygen species, antimicrobial and anti-inflammatory activities are herein presented. Based on the chemical profile obtained using HPLC, OP was grouped into seven variants (OP1–OP7) and all of them exhibited high scavenging activity, mainly against superoxide and hypochlorous acid species. OP1, OP2, and OP3 had the smallest minimal inhibitory concentration (MIC) against Gram-positive bacteria Streptococcus mutans, Streptococcus oralis, and Streptococcus aureus (12.5–100 μg/mL). OP1, OP2, OP3, and OP4 were more effective against Pseudomonas aeruginosa (Gram-negative), with MIC values ranging from 100 to 200 μg/mL. OP6 showed anti-inflammatory activity by decreasing NF-kB activation and TNF-α release in RAW 264.7 macrophages, and expressing the NF-κB-luciferase reporter stable gene. Therefore, south Brazilian OP can be considered an excellent source of bioactive compounds with great potential of application in the pharmaceutical and food industry.
- Published
- 2016
7. Concentração de ácido eicosapentaenóico (EPA) e ácido docosahexaenóico (DHA) em peixes marinhos da costa brasileira
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Masaharu Ikegaki, Yong Kum Park, Jesuí Vergílio Visentainer, and Patrícia de Oliveira Carvalho
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eicosapentaenoic acid ,Chemistry ,lcsh:TX341-641 ,EPA ,docosahexaenoic acid ,fishes ,peixes ,Aquatic organisms ,DHA ,ácidos graxos ,lcsh:Technology (General) ,ácido graxo eicosapentaenóico ,ácido graxo docosahexaenóico ,lcsh:T1-995 ,Food science ,fat acids ,lcsh:Nutrition. Foods and food supply ,Food Science ,Biotechnology - Abstract
Análises experimentais foram realizadas objetivando a composição quantitativa dos ácidos graxos Eicosapentaenóico (EPA) e Docosahexaenóico (DHA), em diferentes partes do corpo de espécies de peixes marinhos da costa brasileira (atum, bonito, olho de boi, cavalinha, sardinha e serra). Os teores de EPA e DHA foram analisados em duas partes distintas: olho (órbita ocular e material gorduroso da cavidade ocular) e filés, sendo significativas as diferenças entre as mesmas. Os teores de DHA para uma determinada espécie foram sempre superiores no olho em relação ao filé, sendo o mesmo observado para o EPA em quatro das espécies (olho de boi, cavalinha, sardinha e serra). Comparando-se a mesma espécie e partes do corpo dos peixes, observou-se que os teores de DHA foram superiores aos teores de EPA, exceto para a sardinha. A somatória dos níveis de EPA e DHA em filés foram maiores para as espécies sardinha e bonito, mostrando serem uma boa fonte alimentar destes ácidos, especialmente a sardinha por ser uma fonte com preço acessível no Brasil. Quantitative compositions of two unsaturated fatty acids such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) were examined in fish oil from orbital cavity and fillets of fishes which captured from Brazilian coast. The experimental results demonstrated that quantities of DHA were extremely higher in oil from orbital cavity of Atum and Bonito as compared with fillet oil, whereas orbital cavity oil from Olho de Boi, Cavalinha, Sardinha, and Serra contained slightly higher DHA than fillet oil. On the other hand, fillet oil from Atum and Bonito contained more EPA than oil from orbital cavity, whereas orbital cavity oils from Olho de Boi, Cavalinha, Sardinha and Serra contained higher quantities of EPA than oils from fillets.
- Published
- 2000
8. ESTUDO DA PREPARAÇÃO DOS EXTRATOS DE PRÓPOLIS E SUAS APLICAÇÕES
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Masaharu Ikegaki, José A. da Silva Abreu, Nívia M. Freire Alcici, and Yong Kun Park
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Traditional medicine ,Chemistry ,Propolis ,Food Science ,Biotechnology - Published
- 1998
9. Produção de lactosacarose por β-frutofuranosidase de bacillus sp nº417 a partir de lactose e sacarose
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Yong Kun Park and Masaharu Ikegaki
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chemistry.chemical_classification ,Sucrose ,Chemistry ,Microorganism ,Bacillus sp ,chemistry.chemical_compound ,Invertase ,Enzyme ,Biochemistry ,Food science ,Lactose ,Sugar ,Food Science ,Biotechnology - Abstract
One thousand three hundred and fourty one strains of microorganisms were isolated from soil, flower and fruit samples and examineted for production of b-fructofuranosidase. This enzyme produces lactosucrose from mixture of lactose and sucrose. Six strains of microorganisms were selected as lactosucrose producers. Among them, one strain, identified as Bacillus sp no 417, showed high b-fructofuranosidase transfer activity to lactosucrose production. The optimum pH and temperature for lactosucrose production were 5,6 and 45°C, respectively. The best condition for the lactosucrose production were: 8 hours of reaction; a ratio lactose to sucrose of 1:1 (w/w) and a total sugar concentration of 20% (1:1, w/w).
- Published
- 1997
10. Apolar Bioactive Fraction of Melipona scutellaris Geopropolis on Streptococcus mutans Biofilm
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Marcos Guilherme da Cunha, Pedro Luiz Rosalen, Lívia Câmara de Carvalho Galvão, Marcelo Franchin, Severino Matias de Alencar, Bruno Bueno-Silva, and Masaharu Ikegaki
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chemistry.chemical_classification ,education.field_of_study ,biology ,Article Subject ,Population ,Biofilm ,Biofilm matrix ,lcsh:Other systems of medicine ,Antimicrobial ,biology.organism_classification ,Polysaccharide ,lcsh:RZ201-999 ,Streptococcus mutans ,Microbiology ,Complementary and alternative medicine ,chemistry ,Extracellular ,Food science ,education ,Melipona scutellaris ,Research Article - Abstract
The aim of this study was to evaluate the influence of the bioactive nonpolar fraction of geopropolis onStreptococcus mutansbiofilm. The ethanolic extract ofMelipona scutellarisgeopropolis was subjected to a liquid-liquid partition, thus obtaining the bioactive hexane fraction (HF) possessing antimicrobial activity. The effects of HF onS. mutansUA159 biofilms generated on saliva-coated hydroxyapatite discs were analyzed by inhibition of formation, killing assay, and glycolytic pH-drop assays. Furthermore, biofilms treated with vehicle control and HF were analyzed by scanning electron microscopy (SEM). HF at 250 μg/mL and 400 μg/mL caused 38% and 53% reduction in the biomass of biofilm, respectively, when compared to vehicle control (P<0.05) subsequently observed at SEM images, and this reduction was noticed in the amounts of extracellular alkali-soluble glucans, intracellular iodophilic polysaccharides, and proteins. In addition, theS. mutansviability (killing assay) and acid production by glycolytic pH drop were not affected (P>0.05). In conclusion, the bioactive HF of geopropolis was promising to control theS. mutansbiofilm formation, without affecting the microbial population but interfering with its structure by reducing the biochemical content of biofilm matrix.
- Published
- 2013
11. Biological activities of the fermentation extract of the endophytic fungus Alternaria alternata isolated from Coffea arabica L
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Claudio M. Costa-Neto, Maurette dos Reis Vieira Fernandes, Tales Alexandre Costa e Silva, Ludwig H. Pfenning, Masaharu Ikegaki, Marisa Aparecida de Lima, Tassiela Andréa Heinrich, and Severino Matias de Alencar
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DPPH ,Endophytic fungi ,Compostos bioativos ,lcsh:RS1-441 ,Alternaria alternata^i2^sextrato/atividade antioxida ,medicine.disease_cause ,Alternaria alternata ,Plant use of endophytic fungi in defense ,Bioactive compounds ,Microbiology ,lcsh:Pharmacy and materia medica ,chemistry.chemical_compound ,medicine ,Fungos endofíticos ,Agar diffusion test ,Food science ,General Pharmacology, Toxicology and Pharmaceutics ,Alternaria alternata^i1^sextract/antioxidant activ ,Candida albicans ,biology ,Coffea arabica ,biology.organism_classification ,chemistry ,Staphylococcus aureus ,Fermentation - Abstract
A total of 22 endophytic fungi isolated from coffee (Coffea arabica L.) were cultivated in vitro and their crude extracts tested. The screening was carried out using the agar diffusion method against Staphylococcus aureus, Escherichia coli and Candida albicans. The most effective isolate was Alternaria alternata, and subsequently, its extract was assayed. The total phenolic content was 3.44 μg GAE/mg of the crude extract. For the antibacterial and antifungal activity assays, minimum inhibitory concentrations (MIC) and minimum bactericidal and fungicidal concentrations (MBC and MFC) were determined. The ranges of MIC values were 50-100 μg/mL for S. aureus and 400-800 μg/mL for E. coli. The extract did not show activity in the tested concentrations for C. albicans. The fungal crude extract was assayed for antioxidant activities. Its ability to scavenge DPPH radicals and antioxidant activity by β-carotene/linoleic acid system oxidation was not significant. In addition, antitumor activity was studied using the MTT assay. At a dilution of 400 μg/mL, the extract displayed a cytotoxic activity of approximately 50% towards HeLa cells in vitro. The results indicate that endophytic fungi could be a promising source of bioactive compounds and warrant further study.Total de 22 fungos endofíticos isolados de café (Coffea arabica L.) foi cultivado in vitro e seus extratos testados. A triagem foi conduzida pelo método de difusão em agar contra bactérias Gram-positiva, Gram-negativa e uma levedura. O isolado mais efetivo foi Alternaria alternata e, subsequentemente, seu extrato foi analisado. O conteúdo de fenólicos totais do extrato bruto foi de 3,44 μg EAG/mg de extrato. Para os testes de atividade antimicrobiana, a concentração inibitória mínima (CIM) e concentração bactericida e fungicida mínima (CBM e CFM) contra Staphylococcus aureus, Escherichia coli e Candida albicans foram determinadas. Resultados da CIM variaram entre 50-100 μg/mL para S. aureus e 400-800 μg/mL para E. coli. O extrato bruto não apresentou atividade nas concentrações testadas para C. albicans. Foram analisadas as atividades antioxidantes do extrato bruto. Sua habilidade para seqüestrar radicais DPPH e a atividade antioxidante pela oxidação do sistema β-caroteno/ácido linoléico não foram significativas. Além disso, a atividade antitumoral foi estudada pelo teste do MTT. À diluição de 400 μg/mL, o extrato apresentou atividade de aproximadamente 50% sobre as células HeLa in vitro. Os resultados indicam que fungos endófitos poderiam ser uma fonte promissora de compostos bioativos necessitando de estudos futuros.
- Published
- 2009
12. Bioassay guided purification of the antimicrobial fraction of a Brazilian propolis from Bahia state
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Severino Matias de Alencar, Rogéria Cristina Zauli, Mary Ann Foglio, Pedro Luiz Rosalen, Myrella Lessio Castro, Vera Lúcia Garcia Rehder, Walquíria Ribeiro Vilela, and Masaharu Ikegaki
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Staphylococcus aureus ,Chromatography, Gas ,Microbial Sensitivity Tests ,High-performance liquid chromatography ,Propolis ,Streptococcus mutans ,Benzophenones ,chemistry.chemical_compound ,Minimum inhibitory concentration ,Animals ,Food science ,Chromatography, High Pressure Liquid ,Minimum bactericidal concentration ,Fatty Acids ,lcsh:Other systems of medicine ,General Medicine ,Bees ,lcsh:RZ201-999 ,Antimicrobial ,Bioactive compound ,Anti-Bacterial Agents ,Complementary and alternative medicine ,chemistry ,Biochemistry ,Gas chromatography ,Antibacterial activity ,Brazil ,Research Article - Abstract
Background Brazilian propolis type 6 (Atlantic forest, Bahia) is distinct from the other types of propolis especially due to absence of flavonoids and presence of other non-polar, long chain compounds, but presenting good in vitro and in vivo antimicrobial activity. Several authors have suggested that fatty acids found in this propolis might be responsible for its antimicrobial activity; however, so far no evidence concerning this finding has been reported in the literature. The goals of this study were to evaluate the antibacterial activity of the main pure fatty acids in the ethanolic extract and fractions and elucidate the chemical nature of the bioactive compounds isolated from Brazilian propolis type 6. Methods Brazilian propolis type 6 ethanolic extract (EEP), hexane fraction (H-Fr), major fatty acids, and isolated sub-fractions were analyzed using high performance liquid chromatography (HPLC), high resolution gas chromatography with flame ionization detection (HRGC-FID), and gas chromatography-mass spectrometry (GC-MS). Three sub-fractions of H-Fr were obtained through preparative HPLC. Antimicrobial activity of EEP, H-Fr, sub-fractions, and fatty acids were tested against Staphyloccus aureus ATCC 25923 and Streptococcus mutans Ingbritt 1600 using minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Results EEP and H-Fr inhibited the growth of the microorganisms tested; nevertheless, no antimicrobial activity was found for the major fatty acids. The three sub-fractions (1, 2, and 3) were isolated from H-Fr by preparative HPLC and only sub-fraction 1 showed antimicrobial activity. Conclusion a) The major fatty acids tested were not responsible for the antimicrobial activity of propolis type 6; b) Sub-fraction 1, belonging to the benzophenone class, was responsible for the antimicrobial activity observed in the present study. The identification of the bioactive compound will improve the development of more efficient uses of this natural product.
- Published
- 2009
13. Composição fenólica, atividade antibacteriana e antioxidante da própolis vermelha brasileira
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Masaharu Ikegaki, Tatiane Luiza Cadorin Oldoni, Adna Prado, Rosângela Maria Neves Bezerra, Severino Matias de Alencar, Pedro Luiz Rosalen, and Ingridy Simone Ribeiro Cabral
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Chloroform ,Antioxidant ,Chromatography ,antioxidant ,medicine.medical_treatment ,General Chemistry ,Propolis ,chemistry.chemical_compound ,chemistry ,antibacterial activity ,medicine ,Food science ,red propolis ,Chemical composition - Abstract
Propolis is a resinous hive product collected by honeybees from various plant sources. It has a complex chemical composition, constituted by various phenolic compounds. Extracts of increasing polarity (n-hexane, chloroform, and ethanol) were obtained from a sample of red propolis from the state of Alagoas. Assays were carried out for determination of contents of phenolics, along with antibacterial and antioxidant activities. The EEP, fractions and sub-fractions showed strong biological activities and were related with phenolic the content compounds contents. The sub-fractions were more bioactive than the EEP and fractions, demonstrating that the antioxidant and antibacterial activities are not a result of synergistic effect between the various chemical compounds in propolis.
- Published
- 2009
14. Determinação da concentração de beta-glucano em cogumelo Agaricus blazei Murill por método enzimático
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Yong K. Park, Severino Matias de Alencar, Claudio Lima de Aguiar, and Masaharu Ikegaki
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beta-glucan ,enzymatic determination ,Agaricus blazei Murill ,Antioxidant ,medicine.medical_treatment ,Agaricus blazei ,Polysaccharide ,Beta-glucan ,chemistry.chemical_compound ,Human health ,medicine ,T1-995 ,TX341-641 ,Food science ,determinação enzimática ,Technology (General) ,beta-glucano ,chemistry.chemical_classification ,Protease ,Nutrition. Foods and food supply ,Enzyme ,chemistry ,Biochemistry ,Acid hydrolysis ,Food Science ,Biotechnology - Abstract
Cogumelos comestíveis contêm importantes propriedades funcionais. Em particular, beta-glucanos, homo- e hetero-glucanos com ligações glicosídicas beta(1->3), beta(1->4) e beta(1->6), supostamente responsáveis por algumas propriedades benéficas à saúde humana, como atividade imunomodulatória, antioxidante, antiinflamatória e anticancerígena. Neste trabalho, a quantidade de beta-glucano presente em cogumelo Agaricus blazei Murill, coletados de três diferentes locais, foi analisada utilizando-se método enzimático. As amostras (em base seca) foram tratadas com alfa-amilase, protease bacteriana e com glicoamilase fúngica. beta-glucano foi quantificado após hidrólise ácida e determinação da glicose liberada. Foi verificado que amostras de A. blazei Murill cultivadas em estufas apresentaram menor concentração de b-glucano (8,4±0,9 e 7,6±2,8g/100g) quando comparado com amostras cultivadas em campo aberto (10,1±2,1g/100g). Observou-se ainda que, mesmo sendo cultivado em condições semelhantes, porém em locais diferentes, as amostras apresentaram diferenças significativas (7,6±2,8 e 8,4±0,9g/100g). Edible mushrooms contains a very interesting functional properties. Among them, the beta-glucans, polysaccharides with beta-1,3; b-1,4 and beta-1,6glucosidic linkages, they are responsible to a series of properties to human health, such as immunomodulatory, antioxidant, antiinflammatory and, antitumoral activities. In the present work, the Agaricus blazei Murill beta-glucan concentrations from three locations, were determined through the enzymatic method. Samples were treated with alpha-amylase, bacterial protease and fungal glucoamylase. beta-glucans were quantified after acid hydrolysis and, the glucose determined for spectrophotometric method. It was verified that samples cultivated inside stoves presented smaller beta-glucan concentration (8.4±0.9 and 7.6±2.8g/100g), when compared with samples cultivated in open field (10.1±2.1g/100g). It was also observed that the samples cultivated in similar conditions, even so in different place, presented significant differences (7.6±2.8 and 8.4±0.9g/100g).
- Published
- 2003
15. Effect of a novel type of propolis and its chemical fractions on glucosyltransferases and on growth and adherence of mutans streptococci
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Pedro Luiz Rosalen, William H. Bowen, Hyun Koo, Simone Duarte, Masaharu Ikegaki, Jaime Aparecido Cury, and Mitsue Fujimaki Hayacibara
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Pharmacology ,Minimum bactericidal concentration ,biology ,Chemistry ,Pharmaceutical Science ,General Medicine ,Microbial Sensitivity Tests ,Propolis ,biology.organism_classification ,Streptococcus mutans ,Streptococcus sobrinus ,Bacterial Adhesion ,Microbiology ,Minimum inhibitory concentration ,Glucosyltransferases ,biology.protein ,Humans ,Glucosyltransferase ,Agar diffusion test ,Food science ,Enzyme Inhibitors ,Antibacterial activity - Abstract
Flavonoids have been considered the main biologically active components in propolis. However, a new variety of flavonoid-free propolis was recently found and chemically classified as type 6. Because it showed activity against oral microorganisms, this study evaluated the effects of the crude ethanolic extract of this propolis and its chemical fractions on the activity of purified glucosyltransferases (GTFs) and on the growth and adherence of mutans streptococci. The inhibitory effect of propolis extracts on GTF activities was determined either in solution or adsorbed onto saliva-coated hydroxyapatite. Streptococcus mutans Ingbritt 1600, Streptococcus sobrinus 6715, and two clinical isolates of each species were used for antibacterial assays. Susceptibilities to the test extracts were analyzed using the agar diffusion method and by determining the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC); the effect on bacterial adherence to a glass surface was also assessed. The activity of GTFs in solution was effectively inhibited by the ethanolic extract of propolis type 6 (EEP) (>80% inhibition at 0.5 mg/ml), hexane, and chloroform fractions (60-90% inhibition at 100 microg/ml); their inhibitory effects on surface enzymes were less pronounced. The EEP, hexane, and chloroform fractions also showed significant antibacterial activity. The data showed that propolis type 6 remarkably reduced GTF activity and inhibited mutans streptococci growth and adherence; these biological activities are associated with its nonpolar components.
- Published
- 2003
16. Effect of a new variety of Apis mellifera propolis on mutans Streptococci
- Author
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Pedro Luiz Rosalen, Yong K. Park, Ramiro Mendonça Murata, Masaharu Ikegaki, Hyun Koo, Regiane Yatsuda, Jaime Aparecido Cury, Severino Matias de Alencar, and Gláucia Maria Bovi Ambrosano
- Subjects
Sucrose ,Flavonoid ,Applied Microbiology and Biotechnology ,Microbiology ,Bacterial Adhesion ,Propolis ,Streptococcus mutans ,Minimum inhibitory concentration ,Potency ,Animals ,Food science ,Glucans ,Chromatography, High Pressure Liquid ,Antibacterial agent ,chemistry.chemical_classification ,Antiinfective agent ,biology ,General Medicine ,Bees ,biology.organism_classification ,stomatognathic diseases ,chemistry ,Glucosyltransferases ,Spectrophotometry ,Antibacterial activity - Abstract
The effects of a new variety of propolis, from Northeastern Brazil (BA), on growth of mutans streptococci, cell adherence, and water-insoluble glucan (WIG) synthesis were evaluated. Propolis from Southeastern (MG) and Southern (RS) Brazil were also tested as an extension of our previous work. Ethanolic extracts of propolis (EEP) were prepared and analyzed by reversed-phase HPLC. For the antibacterial activity assays, minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) of EEPs against Streptococcus mutans, S. sobrinus, and S. cricetus were determined. Cell adherence of S. mutans and S. sobrinus to a glass surface was measured spectrophotometrically at 550 nm. WIG synthesized from sucrose by glucosyltransferase (Gtf) was extracted and quantified by the phenol-sulfuric method. The HPLC profile of the new variety of propolis was entirely different from Southeastern and Southern propolis. Neither flavonoid aglycones nor p-coumaric acid were detected in EEP BA. All EEPs demonstrated biological activities against mutans streptococci; EEP BA showed the highest potency in all in vitro parameters evaluated in this study. The ranges of MIC values were 50 (EEP BA)-400 microg/ml (MG), for S. mutans; and 25 (BA)-400 microg/ml (MG), for S. sobrinus and S. cricetus. The bactericidal concentration of EEPs was four to eight times the MIC values. The adherence of S. mutans and S. sobrinus cells and WIG synthesis were markedly inhibited by EEPs, demonstrating significant inhibition at all concentrations compared with the control (80% ethanol) (p0.05). EEP BA showed 80% inhibition of cell adherence and WIG synthesis at concentrations as low as 12.5 and 7.8 microg/ml, respectively. The results show that the new variety of propolis was exceptionally effective in all in vitro parameters tested against mutans streptococci; biological effects of propolis are likely not to be due solely to flavonoids and (hydroxy)cinnamic acid derivatives.
- Published
- 2000
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