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The objective of this work was to study the effect of seaweed (Eucheuma cottonii) our addition on physicochemical and sensory characteristics of an Indonesian-style beef meatball. Seaweed flour (SF) was added to meatball batter at 0% (CON), 2.5% (SF2.5), 5.0% (SF5) and 7.5% (SF7.5) in weight/meat weight (w/w) basis. Proximate composition (moisture, crude protein, crude fat, crude fiber and ash content), pH, cooking loss, hardness, water-holding capacity, instrumental color and sensory properties (color, texture, aroma, taste and overall acceptability) of the boiled meatballs were evaluated. The effect of seaweed our addition was very significant (P

Snakehead fish (Channa striata), a freshwater fish, is known to have functional properties in helping the process of healing burns and wounds after surgery. Besides that, the use of seaweed as a source of food fiber is also widely reported. This research was conducted to design fiber-rich fish nuggets. The purpose of this study was to determine the maximum levels of seaweed substitution in the processing of snakehead fish nuggets based on sensory properties. Single-factor experiment (seaweed, Eucheuma cottonii, substitution) arranged in a Completely Randomized Design with four replications was applied in this study. The parameters observed were hedonic and hedonic quality sensory properties for the attributes of color, aroma, texture, and taste of nuggets. The data obtained were analyzed by the Friedman Test, followed by the Wilcoxon Rank Test to detect the differences between treatments. Treatment of up to 75% seaweed substitution (0, 25, 50, and 75%) decreases significantly (p 0.05) the hedonic sensory acceptance of the snakehead fish nuggets for all attributes except the texture which shows an increase. Whereas, the treatment with substitution of seaweed in the narrow percentage of 0, 5, 10, 15, and 20% gave an expected result, which showed that there was no significant difference (p 0.05) of the hedonic sensory response for the overall acceptance. Nevertheless, 15% of seaweed substitution provides the best hedonic sensory response for overall acceptance. These results indicate that 15% of seaweed (Eucheuma cottonii) substitution is recommended for use in the processing of snakehead fish nuggets.

The objective of this study was to observe the interactions between salt-soluble proteins from beef and seaweed (Eucheuma cottonii) flour and its effect to the quality of the produced protein gel. The study was conducted as experimental research with two factorials completely randomized design. The effect of different NaCl (2.0%; 2.5%; 3.0%; and 3.5%) and Eucheuma cottonii flour (5%; 10%; and 15%) concentrations was observed to the physical and proximate quality of the protein gel matrix. The observed gel quality variables include pH, gel strength, color (L, a*, and b*), moisture, protein, and fat content. The gel was gelatinized at 90°C for 20 min and cooled down at 4°C for 12 h. The results showed that there was no interaction effect found on all of observed variables. However, a highly significant effect (P

Eucheuma cottonii is red algae that contain high carbohydrates, especially carrageenan. Carrageenan is a sulfated polygalactan with 15-40% of ester-sulfate content. It is formed by alternate units of D-galactose and 3,6-anhydro-galactose joined by α-1,3 and β-1,4-glycosidic linkage. The galactose polymer therein can be hydrolyzed into Galactooligosaccharide (GOS)type prebiotics. Hydrolysis was done by low concentration of sulfuric acid and crude enzyme from inactivation of Vibrio algynoliticus cell broth. Combination of prebiotic and probiotic provide lactic acid bacteria (BAL) as well as its food sources in the colon. The aim of this research is to know the characteristics of BALgrowth and fermentation in hydrolysateof E. cottonii and to know the best method in producing synbiotic powdersfrom BAL grown in hydrolysate. Analysis conducted include the characteristics of synbiotic powders using two types of BAL, namely Lactobacillus casei and kefir bacteria (Lactobacillus spp.). Characteristics of the two types of BAL produced during fermentation were qualified as probiotics which were amounted to more than 108 cell/mL, pH value 0.2%. The results showed that the number of living bacteria was higherin the method of synbiotic productions after dryingof hydrolysates followed by inoculum addition, than the method of synbiotic productions which inoculum addition was donebefore drying. Keywords:Eucheuma cottonii, Lactobacillus casei,Lactobacillus spp.in kefir

Water pollution can make many problems such as, the incidence of disease and poisoning. Pollution in water can produce free radicals and it is can trigger of disease for aquatic organisms. There is some efforts that can be done to provide this problem, such as chemical compound that can reduce the reaction of free radicals. Antioxidants are one of the chemical compounds that can reduce the activity of free radicals. Eucheuma cottonii is the one of a seaweed that has many in antioxidant compounds, such as phenol compounds, but it is also rich in iodine fiber and other important minerals. The method used in this research is descriptive explorative and experimental method. This research was conducted with several stages of seaweed extraction. Identification of Eucheuma cottonii extract is using FTIR test. The last stage is an antioxidant activity test that includes DPPH test (1,1-diphenyl-2-picrilhidrazil) and Inhibition Concentration 50 (IC 50). The results obtained in this study were based on FTIR test of antioxidant compound in Eucheuma cottonii seaweed extract. The one of compounds that have antioxidant activity include galaktosa-4-sulfat. Based on the results of antioxidant activity test using DPPH obtained that seaweed extract Eucheuma cottonii active as an antioxidant to ward off free radicals in the waters. The concentration of Eucheuma cottonii seaweed extract for preventive 50% concentration of DPPH is 39,926 ppm.

Bioethanol is one of the renewable resources derived from sugar fermentation process of carbohydrate substance with microorganismaid. Hydrolysis is the stage to get the simple sugar and is done with using k-carragenasethat is obtained from the isolation result of marine microbe in E.cottonii’s habitat. The objectives of this research were to gain time hydrolysis to produce the enzyme activity and reducing sugar highs by k-karagenase and to determine the best of the substrate concentration and k-carragenasein producing a reduction sugar and the highest ethanol. The research steps was started from isolate rejuvenation of IH22 microbe, enzyme production of crudeextraction k-carragenase and 80% acetone precipitation, determination of the hydrolysis timeand activity of k-carragenase. The next step was the substrate hydrolysis with amounts of 6%, 9%, and 12% (b/v) solids, by enzyme from acetone concentrations of 0%, 2.5%, and 5% (v/v) and then they were fermented for six days with the S.cereviseaeadapted yeast extract. Thek-carragenasse highest enzyme activity was obtained in incubation for 30-60 minutes while the highest reduction sugar was obtained inhydrolysis time for 120 minutes. During the hydrolysis,12% substrate concentration generated the highest reduction sugar and whole sugar of 3.21% and 9.89%, respectively, while 9% substrate with 5% enzyme treatment contained1.49% reduction sugar and 2.75% whole sugar, which was the best substrate in producing ethanol during fermentation process of 1.23% (v/v) and 0.82% (b/v) with 55.78% substrate efficiency and 70.44% fermentation efficieny.Keywords : bioethanol, Eucheuma cottonii, k-carragenasse, S.cerevisiae

Kekian is a processed product from fish meat added with seasoning, fillers and binders then formed and wrapped with tofu skin. Kekian has a low dietary fiber so that the addition of seaweed porridge can be used to increase the content of dietary fiber in kekian. The purpose of this study was to determine the effect of adding different seaweed porridge (Eucheuma cottonii) to characteristics of manyung (Arius thalassinus) kekian and determine the best seaweed porridge to the best kekian fish characteristics. The research method used was experimental laboratories using a completely randomized design of one factor is concentration of the addition of porridge (0%, 20%, 30% and 40%) with 3 replications. Sensory value data were analyzed by the Kruskal-Wallis test, while tests of dietary fiber, air content, protein content, water holding capacity and gel strength were analyzed using ANOVA. The results showed that the difference in the addition of seaweed porridge had a significant effect (P

The edible red seaweed Eucheuma cottonii is widely cultivated throughout most of the tropical seas. Asthma affects over 300 million people worldwide and is related to airway inflammation, smooth muscle hypertrophy, mucous hyperplasia and hyper-secretion, and airway constriction. The therapeutic mechanisms by which Eucheuma cottonii extract (SECE) reduced symptoms in experimental ovalbumin-induced allergic asthma animal model was compared to the FDA approved anti-histamine drug Loratadine. The extract significantly ameliorated nasal itching, sneezing, lung histopathology, immunohistochemistry and related genes changes. The SECE was anti-inflammatory and altered the circulating white blood cell levels. The SECE treatment decreased mucin synthesis, and downregulated the tumour necrosis factor-alpha ( TNF-α ), interleukin (IL)-4, nuclear factor kappa beta ( NF-κB ), epidermal growth factor receptor ( EGFR ) and matrix metalloproteinase ( MMP )-9 expressions, but increased the interferon-gamma ( IFN-γ ) expression. The lectins-rich extract (FTIR analysed) suppressed asthmatic inflammation, lung eosinophil infiltration, and mucin synthesis, and reduced the bronchiole smooth muscle thickness to facilitate airway flow.

Euchema cottonii is a good source of kappa-carrageenan and can be found cultivated in the Indonesia coastal areas in which one of them is in Banten Province. Carrageenans have many applications and are utilized in human food and pet-food industry. Carrageenans are also utilized in non-food industry such as pharmaceuticals, cosmetics, printing and textile formulations. Hence, the present study features on the cooking process cooking time and cooking temperature. The effects of these parameters on carrageenan quality such as gel viscosity and gel strength were studied. The process of extraction of carrageenan was conducted with variations temperature: 60, 70, and 80 °C and the variation of time: 1, 2, and 3 hours. Alkaline substance used was KOH with 8% concentration and the ratio of solvent to dry seaweed 8:1. From the present investigation, it was observed that SRC extraction reached the best condition at temperature 70 °C for 2 hours with the value of yield 30.20%, 5.90% moisture content, 18.34% ash content, sulfate content of 6.94%, viscosity of 190 cP, and the gel strength 714.45 g / cm2. The treatment of temperature and extraction time significantly affected the quality of the SRC yield parameter, viscosity and gel strength.

The presence of inhibitors such as hydroxymethyl furfural (HMF) and dissolved salts on the hydrolysate disrupt the growth of Saccharomyces cerevisiae in fermentation process. The objective of research was to increase the yield of ethanol in fermentation process through neutralization and detoxification of Eucheuma cottonii hydrolysate. Hydrolysate was neutralized and overliming with Ca(OH)2 and NH4OH and continued by activated charcoal adsorption. The results showed that the highest content of reducing sugars was 11.34% (w/v) on neutralization with Ca(OH)2. The lowest content of HMF was 1.39 g/kg hydrolysate on overliming treatment with Ca(OH)2. Overliming treatment with Ca(OH)2 was able to reduce levels of HMF up to 16.77%, while the treatment with NH4OH overliming contributed to 1.06% for the HMF reduction. The highest ethanol yield was 2.49% (v/v) through neutralization with Ca(OH)2, while neutralization with NH4OH treatment was 0.49% (v/v). The low yield of ethanol on treatment with NH4OH due to inhibition by HMF and high levels of salinity in the hydrolysate, 1.89 g/kg hydrolysate and 1600/00 respectively. The fermentation efficiency obtained at treatment by neutralization with Ca(OH)2 was 56.14%.

Alkali treated cottonii (ATC) is a derivative product of Eucheuma cottoniithat is treated with alkali. This study used raw material of sap-free seaweed for ATC processing. Sap-free seaweed is a seaweed which thallus has been extracted with liquid known as sap. The use of sap-free seaweed as an ATC product is constrained by its low quality, so an effort to improve its quality is needed. Therefore the obejcetive of this Research was to get the optimal conditions of ATC processing. Experimental data were designed and analyzed using Response Surface MethodologyCentral Composite Design (RSM-CCD) using Design Expert 10.0.7® program. The optimization of ATC processing involved three components that were considered influential, namely KOH concentration, temperature, and processing time. Recommendation for optimal conditions issued by RSM-CCD on ATC processing from sap-free seawee d were 6 % KOH concentration at 75 oC for 120 minutes (93.1% desirability). The results of the response analysis showed a yield of 39.47% and a gel strength of 595.32 g/cm2. As a research control, the recommendation of RSMCCD used in ATC processing from nonsap-free seaweed was obtained yield of 36.81% and gel strength of 574.44 g/cm 2. ATC from sap-free seaweed has higher yield and gel strength than that from nonsap-free seaweed. Thismight be due to the sap-free seaweed was obtained using the proper sap extraction process and ATC processingunder optimal conditions, so that the quality of seaweed was better maintained.

Title (Bahasa Indonesia): Efektifitas polisakarida yang diekstrak dari alga, Euchema cottonii , terhadap respon imun ikan nila ( Oreochromis niloticus ) This study was aimed to test the effectiveness of polysaccharide extracts of algae, Eucheuma cottonii, in increasing the nonspecific immune response and to get an extract with best efficacy on the immune response of the tilapia, Oreochromis niloticus . The design used completely randomized design (CRD) using 4 treatments, 0 mg/kg of saline material (A), 10 mg/kg of E. cottonii extract (B), 10 mg/kg of Iota (C), and 100 cells/ml of formaline killed vaccine (FKV), Aeromonas hydrophilla , each of which were with 3 (three) replications. The fish were acclimated for 2 weeks, and then treated for 4 weeks. Test animals were vaccinated through intraperitoneal injection 2 times at one week interval as much as 0.2 ml FKV taken with disposable plastic 1 ml syringe. The vaccination was done injecting the bacteria, A. hydrophilla , previously killed in 0.4% formaldehyde FKV at the density of 109 cells / ml. Then the test fish were injected with the test material extract solution. E. cottonii as much as 0.2 ml / fish. Injection point was between the pelvic fins and the lateral line near the anus. The results showed that administration of the polysaccharide extracted from algae in tilapia gave significant effect on nonspecific immune response increment of the fish (total leukocytes and phagocytic activity). Penelitian ini bertujuan untuk menguji keefektifan ekstrak polysakarida alga laut, Eucheuma cottonii, dalam meningkatkan respons kebal non-spesifik dan untuk mendapatkan suatu ekstrak dengan kualitas terbaik terhadap respons kebal ikan nila, Oreochromis niloticus . Penelitian ini mengguna-kan rancangan acak lengkap (RAL) dengan 4 perlakuan dan 3 ulangan. Perlakuan yang digunakan adalah A = 0 mg.kg -1 untuk bahan uji saline ; B = 10 mg.kg -1 ekstrak alga E. cottonii ; C = 10 mg.kg -1 untuk iota; D = FKV A. hydrophilla 10 9 sel/ml. Ikan nila pertama-tama dipelihara dalam kolam (2 x 1 m) selama 2 minggu untuk proses aklimatisasi dan kemudian diberi perlakuan selama 4 minggu. Hewan uji divaksinasi dengan injeksi secara intraperitoneal (IP) dilakukan 2 kali dengan selang waktu seminggu sebanyak 0,2 ml FKV yang diambil dengan plastic syringe disposable 1 ml. Vaksinasi dilakukan dengan menginjeksikan bakteri A. hydrophilla yang telah dimatikan dengan formalin 0,4% FKV pada kepadatan 10 9 sel/ml. Kemudian ikan uji disuntik dengan bahan uji larutan ekstrak E. cottonii dengan dosis penyuntikan 0,2 ml larutan/ikan. Titik suntik diantara sirip perut dan lateral line dekat anus.Hasil penelitian menunjukkan pemberian polisakarida yang diekstrak dari alga pada ikan nila memberi pengaruh yang nyata terhadap peningkatan respon imun nonspesifik ikan (total leukosit dan aktivitas fagositosis).

The aim of this research was to produce high reduction sugar component of bioethanol fermentation substrate using E. cottonii seaweed. The dried E. cottonii was taken from Buton district, South East Sulawesi. The seaweed was hydrolyzed using cellulose for 24hours with various enzyme concentrations (19, 36, and 52 AU) and temperatures (40 and 50°C). The reduction sugar was analyzed by Nelson-Somogy Method then statistical significance test (t-test) was processed by using SPSS software. The results showed that the reduction sugar was 8.045mg/mL, obtained during 12hours of hydrolysis process using 36 AU cellulose at 50°C. However, this hydrolysis result was not significantly different (tested by t-test analysis) with the result shown by 19 AU cellulose enzyme hydrolysis at 50°C temperature which produce 7.937mg/mL of reduction sugar component.