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The nondestructive detection of kiwifruit texture has become an important necessity that influences kiwifruit economic efficiency and consumer recognition of the kiwifruit. This study investigated the feasibility of using hyperspectral imaging technology to identify textural characteristics of kiwifruit, and to establish the model with best performance. Firstly, a near-infrared hyperspectral online grading system (1000–2500 nm) was constructed to acquire spectral images. Textural characteristics were measured via three different textural analysis tests: textural profile analysis, puncture test, and shear test. Then, samples were divided into a calibration set and a prediction set based on rank sampling at a ratio of 3:1. Mean centralization, standard normal variate, and multiplicative scatter correction methods were applied to pre-process obtained spectra. Finally, the partial least squares method was used to establish prediction models of kiwifruit textural characteristics. The models of hardness1, chewiness, resilience, peel hardness, average hardness, and corrected hardness achieved good performance. Both the correlation coefficients of calibration (rc) and prediction (rp) values exceeded 0.9. The difference between the root mean square error (RMSE) of calibration and prediction was small, and the ratio of prediction to deviation (RPD) value exceeded 2, which was used to predict kiwifruit quality. However, the model of average shear force had a low accuracy with an RPD of 1.680 and the model of shear force also had an RPD below 1.5 and a low RMSE. The results showed that near-infrared hyperspectral imaging technique can be used as a nondestructive method for the textural characteristics kiwifruit.