1. Guanosine tetraphosphate ( <scp>ppGpp</scp> ) accumulation inhibits chloroplast gene expression and promotes super grana formation in the moss Physcomitrium ( Physcomitrella ) patens
- Author
-
Seddik Harchouni, Samantha England, Julien Vieu, Shanna Romand, Aicha Aouane, Sylvie Citerne, Bertrand Legeret, Jean Alric, Yonghua Li‐Beisson, Benoît Menand, Benjamin Field, Luminy Génétique et Biophysique des Plantes (LGBP), Institut de Biosciences et Biotechnologies d'Aix-Marseille (ex-IBEB) (BIAM), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Institut de Biologie du Développement de Marseille (IBDM), Aix Marseille Université (AMU)-Collège de France (CdF (institution))-Centre National de la Recherche Scientifique (CNRS), Institut Jean-Pierre Bourgin (IJPB), AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Environnement, Bioénergie, Microalgues et Plantes (EBMP), CEA Irtelis PhD fellowship, APEX grant from the Provence-Alpes Cote d'Azur Region, ANR-15-CE05-0021,SIGNAUXBIONRJ,Manipulation des voies de signalisation de l'énergie afin d'améliorer la production de .lipides chez les eucaryotes photosynthétiques(2015), ANR-17-EURE-0007,SPS-GSR,Ecole Universitaire de Recherche de Sciences des Plantes de Paris-Saclay(2017), and ANR-10-INBS-0004,France-BioImaging,Développment d'une infrastructure française distribuée coordonnée(2010)
- Subjects
Chloroplasts ,photosynthesis ,thylakoid ,Physiology ,growth ,fungi ,Arabidopsis ,Guanosine Pentaphosphate ,food and beverages ,Guanosine Tetraphosphate ,Plant Science ,[SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics ,equipment and supplies ,Thylakoids ,Bryopsida ,moss ,Genes, Chloroplast ,Physcomitrium patens ,bacteria ,guanosine tetraphosphate (ppGpp) ,heterocyclic compounds ,[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology - Abstract
The nucleotides guanosine tetraphosphate and pentaphosphate (or (p)ppGpp) are implicated in the regulation of chloroplast function in plants. (p)ppGpp signalling is best understood in the model vascular plant Arabidopsis thaliana in which it acts to regulate plastid gene expression to influence photosynthesis, plant development and immunity. However, little information is known about the conservation or diversity of (p)ppGpp signalling in other land plants. We studied the function of ppGpp in the moss Physcomitrium (previously Physcomitrella) patens using an inducible system for triggering ppGpp accumulation. We used this approach to investigate the effects of ppGpp on chloroplast function, photosynthesis and growth. We demonstrate that ppGpp accumulation causes a dramatic drop in photosynthetic capacity by inhibiting chloroplast gene expression. This was accompanied by the unexpected reorganisation of the thylakoid system into super grana. Surprisingly, these changes did not affect gametophore growth, suggesting that bryophytes and vascular plants may have different tolerances to defects in photosynthesis. Our findings point to the existence of both highly conserved and more specific targets of (p)ppGpp signalling in the land plants that may reflect different growth strategies.
- Published
- 2022