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The study was conducted to determine whether there is a difference in leaf breaking resistance between genotypes in order to minimize leaf breaks which cause large loss in alfalfa forage production. The experiment was carried out in the Batı Akdeniz Agricultural Research Institute, at the Department of Field Crops in 2016. It was established by randomized complete block design. Breaking resistance of the leaves was measured using a universal testing machine on the main stem belonging to alfalfa genotypes which were collected from 40 different locations at the fourth-end leaves. In addition, thickness of leaf stalk, rupture energy of green and dry samples and rupture stress of green and dry samples were calculated. As a result of the experiment; in dry sample, Demre-1, Döşemealtı-2 and Konyaaltı-2, genotypes were determined to have the highest breaking resistance and Alanya-1, Alanya-2, Finike-1, Finike-2, Manavgat-1 Manavgat-2 and Gazipaşa-2 genotypes were determined to have the lowest breaking resistance. In the dry sample, while the highest breaking resistance value was measured at 1.0419 N, the lowest breaking resistance value was measured at 1.0022 N. A wide variation among genotypes was determined in terms of the characteristics examined and it can be said that the rupture resistance can be used as a selection criterion.