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The research aims to observe the effect of organic fermented cow stool application on the growth of Moringa Oleifera. The research wa conducted at the Bandar Khlifa Village, Percut Sei Tuan district, Deli Sedang District, North Sumatera from September to November 2020. The design used for this research Completely Randomized Design in 4 treatments and 5 replications. The treatments consisted of P0 = Control (Without fertilizer), P1 = 5 ml, P2 = 10 ml, P3 = 15 ml. The parameters observed were plant height, number of stem branches, leaf width and stem diameter. The results showed that the effect of fermented cow stool application had significant effect ( P ≥ 0,05) on increase in plant height, increase in leaf width, and increase in stem diameter, but not significant on increase in number of stem branches, increase in number of leaves,. It is recommended to support good growth (plant height, leaf width and stem diameter) of Moringa Oleifera plants using a 5 ml dose of fermented cow feces fertilization.

The objective of this study was to determine the agronomic diversity of gamma-ray at 50 Gray dose during the phase of embryogenetic callus in napier grass cv Taiwan. This research used a randomized blok design (RBD) of gamma-ray and without radiation elephant grasses (P. purpureum cv Taiwan). There were 51 numbers in irradiated elephant grass, each number three replicated and five plant per number each. Without irradiation control used elephant grass (P. purpureum cv Taiwan); there were 10 clumps in one block and each line number had been repeated three times. Gamma radiation showed no impact on plant height and stem weight (dry weight) at P>0.05, but had a significant effect on number of tillers, leaf length, leaf width, total weight, fresh weight, leaf (dry weight), weight of stem (fresh weight). Gamma ray irradiation selection does not have to generate high output but can be developed on sub optimal soil or extreme weather conditions such as tolerant felling, which requires less high plant. Key words: diversity, forage, irradiation

This study was carried out to investigate the effect of the LED light source on the growth and flowering characteristics in potted chrysanthemum ‘Orange Egg’ during cultivation in a closed growth chamber with a constant temperature of 22℃ and a photoperiod consisted of 11 hours light and 13 hours darkness. Each LED light source of blue (444 nm), red (652 nm), or mixed LED (blue:red:white=2:5:1) was supplied separately. As a result, the plant height of the pot-mum 'Orange Egg' grown in red LED was longest with an average of 9.43 cm. And the plant height in blue LED was shorter than in mixed LED, but there was not statistically significant between them. The plant width and leaf width were also largest in plants grown under red LED similar to the growth response of the plant height. But the number of leaves and leaf lengths were more and longer in monochromatic LED such as blue or red than mixed LED. Flower bud differentiation was completed around 16 to 17 days after short-day treatment regardless of LED light source. However, pot-mum plants grown in red and blue LED did not develop flower buds normally and reach final flowering although those grown in mixed LED were fully flowered around 5 weeks after short-day treatment. Therefore, it is considered that the mixed LED consisting of blue, red and white is useful as LED light source for producing potted chrysanthemums in plant factories.

Mutated genetic resources play a crucial role and are of utmost importance in gene/allele characterization. Currently, hexaploid wheat mutated resources are rarely available. In the present investigation, a mutated population comprising 1,536 EMS-induced mutation lines (M7 generation) of bread wheat (Triticum aestivum L.) were used as an experimental material. Mutant population was developed and maintained at G.B.P.U.A.T, Pantnagar. The experiment was conducted in augmented block design II. in season 2019-20 at Norman E. Borlaug Crop Research Centre G.B.P.U.A.T, Pantnagar. The study revealed that number of tillers per plant recorded significantly higher direct effect on grain yield per plant followed by number of grains per spike, thousand grain weight, plant height, ear length, flag leaf width, grain length, flag leaf area and number of spikelet per spike. The association between grain yield per plant and these characters was significant. The direct negative effects on grain yield was observed by grain width, flag leaf length and peduncle length.

Present study was investigate the effect of different UV-doses on morphological defending system features of two palm species Phoenix dactylifera L. and Washingtonia filifera (Lindl.) H. Wendl. The results showed that the morphological and biomass traits of both palm species grown for 30 days under UV-B with a rate of 2 hrs.day-1 were almost improved. Where, the highest leaf width was recorded (1.1 and 0.7 cm) respectively. Conversely, UV-treatment (4-10 hrs.day-1) has adversely affected on almost morphological and biomass features of both species. Where, the less leaf widths were recorded 0.95, 0.8, 0.73, 0.45, 0.43 and 0.40 cm in both palm species. Besides, results also spotted changes in leaf’s surface appearances. However, present investigation concluded that morphological features and biomass of both species were unaffected with low dose of UV-radiation (2 hrs.day-1). But, date palm (P. dactylifera L.) was capable to survive at high dose of radiation conditions reaches 8 hrs.day-1 for 30 days.

The main roles of plant growth was nutrition. This research aims to determine the concentration of nutrients that can provide the best growth and yield on various mustard. The research method used is a factorial Complete Randomized Design (RBD) with three replications and then analyzed using the Least Significant Difference test (LSD) at a level of 5%. The treatment of vegetable types is as follows: S1 = mustard pakchoy, S2 = mustard samhong, S3 = mustard pagoda, S4 = mustard pakchoy mini. Treatment with nutrition is the AB mix fertilizerzs number of leaves solution as follows: N1 = 1000 ppm, N2 = 1200 ppm, N3 = 1400 ppm, N4 = 1600 ppm, N5 = 1800 ppm. The results showed that plant height, number of leaves, leaf width and wet weight of the pagoda and mini pakchoy plants were not significantly different at 1000 ppm, 1200 ppm, 1400 ppm, 1600 ppm and 1800 ppm. Number of leaves of pakchoy significantly different at 1400 ppm. While the number of leaves and leaf width of samhong plants were not significantly different at each treatment, but were significantly different from plant height at 1600 ppm and significantly different from the wet weight at 1400 ppm

Shoot morphology in rice plants changes in response to P deficiency. However, how genotypic variations in these morphological changes affect the canopy development and biomass production have hardly been explored. The study aimed to identify specific shoot morphological traits that confer biomass production under P deficiency. Four rice genotypes, including AZ-97 (WAS 63���22-5-9-10-1), which exhibits high performance under highly P-deficient fields in Madagascar, were grown for 56 days in flooded pots over several P application rates to simulate P-sufficient and moderately, severely, and extremely P-deficient conditions. AZ-97 had superior shoot P contents and biomass than Takanari, a high-yielding cultivar, and X265, a common high-yielding cultivar in Madagascar at severely to moderately P-deficient conditions. Shoot biomass was highly correlated with projected leaf area (PLA) from the early growth stage, and tiller number, leaf width, and leaf elongation rate explained the variations in PLA. These morphological traits reduced significantly with decreased P application rates, while reduction in AZ-97 was small relative to the other genotypes, even for equivalent shoot P contents. As the result, AZ-97 had greater PLA per unit of shoot P content at equivalent shoot P contents. The result indicates that lower sensitivity and degrees of change in shoot morphology when exposed to P deficiency stress could be a key trait facilitating the maintenance of captured radiation and subsequently influencing genotypic differences in external P uptakes and biomass production. AZ-97 is a potential donor with such traits that can offer an additional avenue for genetic improvement toward P-efficient rice production.Abbreviations DAT: days after transplanting; LN: leaf number in the main stem; PLA: projected green leaf area; PUE: phosphorus use efficiency; SSA: Sub-Saharan Africa.

Selection parameter in coffee breeding for leaf rust (Hemeleia vastatrix) resistance is very important. Breeders used leaf-rust severity and leaf-rust incidence as parameters of direct selection. However, scientific proof is not yet available whether leaf morphology can be used as a parameter of indirect selection. The objective of this research was to seek the possibility of leaf morphology parameter and its ratio to be used as selection criteria through analyses of genotypic and phenotypic correlations of parameter of rust disease and parameter of leaf morphology and its ratio. The result revealed that genotypes showed significant variations in leaf-rust severity (5.21–25.84%), leaf morphology, and leaf-morphology ratio. Leaf length to leaf width ratio, leaf length to leaf area ratio, and leaf width to leaf weight ratio were not affected by the environment. Leaf-rust severity performed highly significant positive genotypic and phenotypic correlations the ratio of with leaf length to leaf area. For selection criteria, leaf-rust severity could be better used rather than leaf-rust incidence and branch-rust incidence. The ratio of leaf length to leaf area could also be used as an indirect selection criterion because the ratio showed a highly significant genotypic correlation with leaf-rust severity (rGab = 0.254**). However, the ratio of leaf length to leaf area is even better chosen for selection criteria rather than leaf-rust severity because the ratio was not affected by the environment. Keywords: fungus, Hemeleia vastatrix, indirect selection

This study was carried out in 2016 to determine the morphological characteristics of the Thymbra spicata L. var. spicata in Siirt Flora. The morphological characteristics of the plant from different aspects (north, south, east, west) and in different developmental stages (before flowering, after full bloom and after flowering) were investigated. The plant samples were collected according to divided parcels in randomized complete blocks design with 4 replications. In the study, different aspects (north, south, east, west) formed the main plots and different developmental stages (before flowering, after full bloom and after flowering) formed the subplots. In the research, while the effects of different aspects and developmental stages on the leaf width were not statistically significant; The effect on plant height, stem thickness, number of flowers, calyx height, petal length and pedicel length were found statistically significant. As a result, it is thought that it would be beneficial to conduct similar studies both in this species and other species of economic value collected from nature.

Thirty six okra genotypes were evaluated for different agro-morphological and biochemical traits at Melkassa Agricultural Research Center, Ethiopia during 2018 main season using 6 x 6 simple lattice design. The objectives were to assess the correlation of agro-morphological and biochemical traits with fruit yield and to partition the correlation in to direct and indirect effects through path analysis. The genotypic correlation was positive and significant for fruit yield per hectare with stem diameter, plant height, leaf length, leaf width, peduncle length, fruit length, fresh fruit mass, hundred seed mass, seed yield per hectare and ash content. The phenotypic correlation was positive and significant for fruit yield per hectare with stem diameter, plant height, number of branches, leaf length, leaf width, peduncle length, fruit length, fresh fruit mass, number of fruits per plant, hundred seed mass, seed yield per hectare, ash, fat and protein content. Path coefficient analysis indicated fresh fruit mass and seed yield per hectare had positive direct effects on fruit yield per hectare at phenotypic and genotypic levels. These traits also exerted high to low positive indirect effects through other traits on fruit yield at genotypic and phenotypic level. In conclusion, this study showed the presence of association of traits with fruit yield indicating that the prime importance of these traits while selecting higher yield okra genotypes.

Purpose In this study, to evaluate the physico-chemical properties of coffee husk compost. Methods The compost process in aerobic condition for 84 days. Physico-chemical parameters were analyzed. In addition, the seed germination, leaf width, stem length and fresh weigh yield of compost were investigated on matured compost using Brassica rapa. Results The obtained results indicate that the pH value 7.72 and EC value 12.54 ms/cm. The total carbon value 37.41%. The total nitrogen value 2.05%. The C/N ratio value 18.2. The seed germination indicate that compost was matured with range 80-100%. On compost yields the optimum doses of the Brassica rapa 3% with leaf width 1.3 cm, stem length 5.1 cm and fresh weight 2.18 gram. Conclusion Generally, the final compost can be served as organic matter for improving soil and plant.

The present investigation entitled “Impact of Plant Bio-regulator on growth and flowering of gerbera (Gerbera jamesonii B.) cv. Goliath” was carried out in premises of Bio-technology cum Tissue Culture Centre, Odisha University of Agriculture and Technology, Bhubaneswar during 2015-16 and 2016-17. The objective of the study was to standardize suitable bio-regulators on flower production in gerbera in open field condition. Apart from control eight treatments of growth regulators were used like GA3 @ 100 ppm, and 150 ppm; Cycocel @ 700 ppm and 800 ppm with or without amino acids as foliar spray. Among vegetative parameters maximum plant spread, leaf length, petiole length, leaf width and leaf area was found in GA3 @ 150 ppm + Amino Acid while maximum leaf number/plant, number of suckers/plant and chlorophyll content in leaf was found in treatment with cycocel @ 700 ppm+ Amino acid. In flowering parameters maximum number of flowers/plant/m2 in a year was found in treatment with cycocel @ 700 ppm + Amino acid.

In barley (Hordeum vulgare L.), lateral branches called tillers contribute to grain yield and define shoot architecture, but genetic control of tiller number and developmental rate are not well characterized. The primary objectives of this work were to examine relationships between tiller number and other agronomic and morphological traits and identify natural genetic variation associated with tiller number and rate, and related traits. We grew 768 lines from the USDA National Small Grain Collection in the field and collected data over two years for tiller number and rate, and agronomic and morphological traits. Our results confirmed that spike row-type and days to heading are correlated with tiller number, and as much as 28% of tiller number variance was associated with these traits. In addition, negative correlations between tiller number and leaf width and stem diameter were observed, indicating trade-offs between tiller development and other vegetative growth. Thirty-three quantitative trait loci (QTL) were associated with tiller number or rate. Of these, 40% overlapped QTL associated with days to heading and 22% overlapped QTL associated with spike row-type, further supporting that tiller development is associated with these traits. Some QTL associated with tiller number or rate, including the major QTL on chromosome 3H, were not associated with other traits, suggesting that some QTL may be directly related to rate of tiller development or axillary bud number. These results enhance our knowledge of the genetic control of tiller development in barley, which is important for optimizing tiller number and rate for yield improvement.

Bu çalışma, farklı turp çeşitlerinin (beyaz, siyah, kırmızı, kırmızı fındık turp) tuz stresi koşullarında tolerans düzeylerini belirlemek ve dışarıdan salisilik asit (SA) uygulamasının çimlenme ve vejetatif gelişim üzerine etkinliğini tespit etmek amacıyla yapılmıştır. Bu amaçla; çimlenme yüzdesi (%), çimlenme süresi, çimlenme indeksi hesaplanmış, ayrıca yaprak sayısı (adet), sürgün boyu (cm), kök uzunluğu (cm), yaprak genişliği (cm), yaprak uzunluğu (cm), gövde çapı (mm), bitki yaş ve kuru ağırlıkları (g) ölçülmüştür. Turp tohumları kontrol, 100 mM, 150 mM ve 200 mM tuz konsantrasyonlarında çimlenmeye tabi tutulmuştur. Tuz stresi çimlenmeyi yüksek oranda inhibe etmemiş ancak bitkilerin vejetatif gelişimlerini olumsuz etkilemiştir. Elde edilen verilerin istatistik analiz sonuçlarına göre 150 mM eşik değer olarak belirlenmiştir. Daha sonra 150 mM tuz konsantrasyonu uygulanan tohumlara farklı dozlarda (0,25, 0,50, 0,75, 1,00 mM) SA ilavesi yapılmış ve çimlenme yüzdeleri açısından tüm çeşitlerde 0,50 mM SA uygulanabileceği sonucuna ulaşılmıştır. Bununla birlikte 1 mM SA uygulaması iri kırmızı ve kırmızı fındık turp çeşitlerinde çimlenmeyi inhibe etmiş, ani ve ciddi çimlenme kayıplarına neden olmuştur (%10, %8, sırasıyla). SA‘in vejetatif gelişim parametreleri üzerine olan etkileri çeşide göre farklılık göstermiş olup, beyaz turp çeşidinde vejetatif gelişim özellikleri açısından inhibe edici olduğu belirlenmiştir. Diğer taraftan siyah ve iri kırmızı turp çeşitlerinde 0,50 mM, kırmızı fındık turp çeşidinde ise 0,75 mM SA uygulaması vejetatif gelişim açısından olumlu sonuç vermiştir.

Common bean is among the most important legume crop for protein source in people’s diet globally and including Kenya. Anthracnose is a common disease of legumes that causes yield loss of up to 90-100%. The aim of the study is to investigate the morphological traits associated with anthracnose resistance in selected common bean genotypes in Kenya. The study was done in three varied agro-ecological zones; University of Eldoret, Bungoma and Busia. Fifteen genotypes were evaluated on field experiment to ascertain morphological traits associated with anthracnose resistance. Field experiment was done in a random complete block design. Data were collected on morphological traits and subjected to analysis of variance in SAS version 9.1. The genotypes, Ciankui, Tasha, KK15, KK8, Miezi mbili and Chelalang showed morphological traits that were significantly (P ≤ 0.05) associated with anthracnose resistance, and also with high grain yields of 1.5 to 2.0 t/ha. Morphological traits associated with common bean anthracnose resistance included Leaf width, leaf length, length of fifth internode of the stems, bracteolate size classification and flower colour. It is recommended that management of anthracnose by use of resistant common bean genotype seeds is essential to provide increased bean yields globally and in Kenya. Key words: Common bean, anthracnose, morphological traits, resistance.

A field trial was conducted to weigh up the path coefficient analysis between various morphological traits which directly as well as indirectly associated with yield of various bottle gourd genotypes. The research experiment consists of total 37 genotypes conducted at Research Farm of Vegetable Science department, Chaudhary Charan Singh Haryana Agricultural University, Hisar during summer season. Genotypes were sown with three replications following (RBD) randomized block design. The observed data were taken for further statistical analysis. Substantial amount of variability was found for the sixteen morphological traits which were confirmed by the ANOVA (analysis of variance). The characters having positive direct effect on yield per ha are number of primary branches, leaf width, nodes to first female flower, days to first female flower opening, days to fruit harvest, number of fruits per vine, fruit yield per plant and weight of seeds.

Gef��rdert im Rahmen des Projekts DEAL, Deutsche Bundesstiftung Umwelt. Grant Number: 20016/464

Sapindus mukorossiGaertn., an important oleaginous woody plant, has garnered increasing research attention owing to its potential as a source of renewable energy (biodiesel). Leaf structural traits are closely related to plant size, and they affect the fruit yield and oil quality. However, plant size factors that predominantly contribute to leaf structural traits remain unknown. Therefore, the purpose of this study was to understand the associations between leaf structural traits and plant size factors in even-aged stands ofS. mukorossi. Results showed that leaf length (LL) and leaf area (LA) markedly increased with the increasing diameter at breast height (DBH) and tree height (TH), although other leaf structural traits did not show noticeable changes. Difference in slopes also indicated that the degree of effect of plant size factors on leaf structural traits was in the order of TH > DBH. Leaf structural traits showed no systematic variation with crown width (CW). LA was significantly positively correlated with LL, leaf width (LW), LL/LW, and leaf thickness (LT) and was significantly but negatively correlated with leaf tissue density (LTD) and leaf dry mass content (LDMC). Specific leaf area showed a significantly negative correlation with LT, LDMC, and LTD. LTD showed a significantly positive correlation with LDMC, but a negative correlation with LT. The results were critical to understand the variability of leaf structural traits with plant size, can provide a theoretical foundation for further study in the relationship between leaf structural traits and fruit yield, and regulate leaf traits through artificial management measures to promote plant growth and fruit yield.

The present study was aimed to assess the extent of genetic diversity with sixty-six genotypes of FCV tobacco (Nicotiana tobacum L.) by using Mahalanobis’ D² statistics at AINP (Tobacco), Zonal Agriculture and Horticultural Research Station, UAHS, Shivamogga, Karnataka during Kharif-2019-20 in RCBD design with two replications. The 66 genotypes were grouped into eight clusters based on D² analysis. The cluster II had maximum with 29 genotypes followed by clusters I, III, V, and IV had the minimum with 16, 16, 8 and 5 genotypes respectively and remaining clusters were solitary. The highest inter cluster distance was observed between cluster VI and VIII and the lowest between clusters II and VIII. Cluster V had exhibited highest intra cluster distance and the lowest was observed in cluster VI, VII and VII. The character days to flowering, top grade equivalent and green leaf yield showed maximum contribution towards total genetic divergence. On the basis of cluster mean, cluster VI was superior for plant height, number of leaves per plant and leaf length. The maximum leaf width was observed in cluster VIII, while cluster VII sowed superiority for days to flowering. The cluster IV showed highest green leaf yield, cured leaf yield and top grade equivalent. Thus, the genotypes involved in these clusters may be taken into consideration for better parents for generating variability for the respective characters and their rational improvement.

Rice (Oryza sativa L.) is one of the most consumed cereal grain in the world constituting the dietary staple food for more than half of the human population. More than 90% of the world’s rice is grown and consumed in Asia. The investigation was carried out with 200 lines of F2 segregating generation of Rice in RCBD design with three replications in kharif 2016. The analysis of variation revealed significant differences in the genotypes for all the characters studied. High heritability was observed in panicle weight/plant followed by thousand grain weight, spikelet density, days to flowering, days to maturity. flag leaf width and thousand grain weight showed maximum genetic advance as percentage of mean followed by stem thickness, flag leaf length, gain yield/plant, spikelet density, number of productive panicles/plant, number of tillers/plant, number of productive tillers/plant, panicle weight/plant, fertile spikelets/plant, stem length, number of spikelets/plants, biological yield. Correlation studies revealed positive and significant association of grain yield/plant with panicle weight/plant followed by panicle index, biological yield/plant, number of tillers/plant, number of productive tillers/plant, number of panicles/plant, harvest index, fertile spikelets/panicle, panicle length, grain breadth, spikelet fertility, flag leaf width, stem length, amylose content. While, it showed negative and significant with days to 50% flowering, thousand grain weight and decorticated grain breadth.

Maize leaf plays an important role in the synthesis of organic compounds. It hosts the interception of light energy and conversion to organic energy, and the capture and assimilation of carbon dioxide in order to produce food. In this study, we explore the heritability of the maize leaf length, leaf width, and the number of leaves of two maize genotypes, JNE and BLC. The estimates of heritability varied from 0.2 to 0.44. Only estimates for leaf width and leaf length are significant. The coefficients of genotypic correlation were between 0.45 and 0.66, and like the estimates of heritability, only the genotypic correlations between parents and offspring are significant for leaf length and leaf width of the two genotypes. The estimates of degree of genetic determination also known as heritability in the broad sense are all high. They range between 0.52 and 0.85. We determined a significance criterion for the degree of genetic determination, the critical value at the level of significance = 0.05. We evaluated three methods for finding the critical value. With two methods based on the algebraic transformation of a function of the Snedecor’s F and a simulation based on that function of F, it can be concluded that the estimates of the degree of genetic determination of leaf width of both the JNE and BLC genotypes are significant. With the third method that is based on fitting a Beta distribution, the critical value helped to conclude that none of the estimates of the degree of genetic determination is significant. The Pearson coefficients of correlation between plant height and all three leaf traits showed significant correlation between them, except leaf length and number of leaves. Key words: Heritability, genotypic correlation, degree of genetic determination, genotype, significance test.

Cultivation of the Elephant Grass cv Taiwan with tissue culture requires an acclimatization stage, therefore this study aims to find the best planting media and the effect of plant age and evaluation of production elephant grass cv taiwan mutant and nutrient content. Acclimatization was carried out in a greenhouse using sand, soil, and a combination of 50% sand with 50% soil, and the mutant type T2, T4 and K4. The acclimation process uses the Factorial RAL method with factor A type of mutant and factor B type of planting media and the parameters assessed are leaf length, number of leaves and plant mortality rate. Production evaluation uses the RAL method and the parameters observed to see elephant grass production are plant height, stem diameter, leaf width, number of tillers and amount of plant biomass production. Proximate analysis is used to show the nutritional value of plants that have been selected individually using scoring assessments. The results of the acclimatization study showed that the planting media that had the lowest mortality rate were sand and soil combination media (22%) followed by soil planting media (27%) and the planting media that had the highest mortality rate was sand media (30%). The results of the production evaluation stage showed that the highest average harvest weight was found in T4 mutants with an average weight of 1353 g clump-1harvest-1, followed by T2 mutants which had an average of 1198 g clump-1harvest-1 and the lowest harvest weight was found in K4 mutants with an average of 1002 g clump-1harvest-1. Based on the research results obtained the best planting media for the acclimation process of elephant grass cv Taiwan mutants is a combination of sand and soil media (1:1). The results of the evaluation stage of T4 mutant production are the best mutants based on morphological performance and production of plant biomass. Proximate analysis of selected plants shows that plants with mutant T2PT7 species have the best nutritional value, with nutritional content of PK 11.71% and SK 24.67%. Keywords : acclimatization, Elephant grass cv Taiwan, proximate analysis

Camelina [Camelina sativa (L.) Crantz] has two distinctive biotypes, summer and winter, with winter biotypes requiring a vernalization treatment to enter the reproductive phase. Increased interest in broadening the diversity of winter-hardy cover crops in the northern Great Plains of the U.S. to reduce soil erosion through the winter months has led seed companies to offer winter camelina seed outsourced from other states. Regrettably, in 2017, outsourced camelina seed from other states turned out to be summer biotypes that did not survive the North Dakota winter. The objectives of this study were to determine the morphological characteristics of seed and seedlings from summer- and winter-biotypes. Morphological characteristics of seedlings were determined by growing nineteen summer- and eleven winter-biotypes in an environmental chamber. The experimental design was a randomized complete block design with three replicates. To determine differences in seed wavelength absorbance between winter- and summer-biotypes both visible and near infrared spectra were examined, which encompass 400–2498 nm wavelengths. Mixtures of cultivars Joelle (winter) and one summer type were analyzed using near infrared spectroscopy (NIRS). Seed mixtures were prepared in increments of 5% of ‘Joelle’. Mixtures of different lots of Joelle were prepared in increments of 25%. Significant interactions were present and observed for pairs of vegetative leaves, growth stage, height, leaf length, leaf width and the number of lobes. The equation for the ratio of winter seed (r2 = 0.96) performed very well, being able to distinguish known field grown cultivars used in the equation. Differences in seed and seedling morphological characteristics can be used to differentiate winter- or summer-biotypes. Having a rapid method to determine the percentage of winter camelina in an unknown seed sample will be very favorable for all producers, as well as seed companies, interested in growing winter camelina as either a winter-hardy cover crop or as a winter annual cash crop.

BackgroundLeaf length and width could be a functioning relationship naturally as plant designs. Single-vein leaves have the simplest symmetrical distribution and structural design, which means that fast-growing single-vein species could interpret the scheme more efficiently. The distribution of leaf length and width can be modulated for better adaptation, providing an informative perspective on the various operational strategies in an emergency, while this mechanism is less clear. Here we selected six age groups ofCunninghamia lanceolatapure forests, including saplings, juveniles, mature, and old-growth trees. We pioneered a tapering model to describe half-leaf symmetric distribution with mathematical approximation based on every measured leaf along developmental sequence, and evaluated the ratio of leaf basal part length to total length (called tipping leaf length ratio).ResultsThe tipping leaf length ratio varied among different tree ages. That means the changes of tipping leaf length ratio and leaf shape are a significant but less-noticed reflection of trees tradeoff strategies at different growth stages. For instance, there exhibited relatively low ratio during sapling and juvenile, then increased with increasing age, showing the highest value in their maturity, and finally decreased on mature to old-growth transition. The tipping leaf length ratio serves as a cost-benefit ratio, thus the subtle changes in the leaf symmetrical distribution within individuals reveal buffering strategy, indicating the selection for efficient design of growth and hydraulic in their developmental sequences.ConclusionsOur model provides a physical explanation of varied signatures for tree operations in hydraulic buffering through growth stages, and the buffering strategy revealed from leaf distribution morphologically provides evidence on the regulation mechanism of leaf biomechanics, hydraulics and physiologies. Our insight contributes greatly to plant trait modeling, policy and management, and will be of interest to some scientists and policy makers who are involved in climate change, ecology and environment protection, as well as forest ecology and management.

Prostrate growth 1 (PROG1) gene is vital in controlling the prostrate growth habit of rice. Studying the effect of PROG1 gene on rice canopy structure is crucial in elucidating the molecular mechanism of rice plant type evolution. Herein, the morphological characteristics of different rice genotypes were collected at different growth stages and leaf nodes using image processing techniques. The morphological characteristics included leaf length, leaf width, and leaf area. The image processing techniques involved boundary mean oscillation (BMO) filtering and minimum bounding rectangle extraction of the target image. On this basis, the effect of the PROG1 gene on rice leaf morphology was quantitatively assessed. Also, the feasibility of image processing techniques in detecting the morphological characteristics of rice leaves was discussed. Under the influence of the PROG1 gene, the length, width, and area of rice leaves decreased by 45.1%, 12.7%, and 44.8%, respectively, at the booting stage. Similarly, the length, width, and area of flag leaves decreased by 15.8%, 32.0%, and 33.7% at the heading stage and by 25.4%, 16.2%, and 19.7% at the filling stage, respectively, and that of secondary leaf reduced by 23.2%, 13.6%, and 54.2% at heading stage and by 24.1%, 17.3%, and 37.0% at filling stage, respectively. Furthermore, the length, width, and area of other leaves reduced by 32.3%, 9.8%, and 51.6% at the heading stage and by 28.6%, 7.3%, and 36.7% at the filling stage, respectively. The leaves in the rice canopy were shorter, narrower, and smaller in leaf area. Notably, no significant differences were found between image processing technology and manual measurement methods regarding the values of leaf morphological characteristics obtained ( P < 0.05 ). Thus, these results show that image processing technology is effective in studying the morphological characteristics of rice leaves. This study provides a reliable foundation for molecular breeding studies and will guide the application of the PROG1 gene in molecular breeding.

Background: Wheat is an important cereal crop and improving wheat production is essential for meeting the food demand from the growing population worldwide. Flag leaf width (FLW) is an important trait affecting plant architecture and contributing to grain yield. To detect loci conferring FLW, we assessed a population of recombinant inbred lines (RILs) from a cross of EGA Wylie/Sumai 3 in different environments.Results: A total of six QTL were detected from the population. Two of them located on chromosome 2B and the other four located on chromosomes 2D, 4B, 7A, and 7B, respectively. The percentage of phenotypic variation (PEV) explained by these loci ranged from 14.6% to 33.8%, with LOD scores varying from 3.01 to 7.81. Of them, the locus located on chromosome arm 7AS is likely novel. Significant effects of this locus were detected in multiple trials conducted and the PEV explained by this QTL varied from 14.6% to 19.8% among the different trials. An orthologous analysis based on rice and Arabidopsis identified 3 putative genes underlying this potentially novel locus.Conclusion: This study identified a stable potentially novel QTL in multiple environments and predicted three candidate genes of it, which laid the foundation for further fine-mapping and cloning the gene(s) underlying QFlw.WS-7A with the contribution to grain yield.

The orchids that produced tuber are known as salep orchids and have been collected from nature for centuries as a medicinal and aromatic plant. These orchids are endangered species because of their limited vegetative and generative production possibilities. Salep orchids, seed germination has been achieved with in-vitro studies, but no source has been found regarding the development of germinated seeds over the years. In this study, Dactylorhiza euxina (Nevski) Czerep. was used as research material. Seeds that were determined to germinate in nature were observed to develop in their own environment for five years and some morphological characteristics (plant height, number of leaves, leaf length, leaf width, total weight, tuber width, and tuber weight) were determined at the end of each vegetation year. Plants that start to develop with seed germination produce one leaf and a tuber of 0.115 g in the first year. When the tubers obtained from the first year were planted again, the plants was obtained 2-3 leaves and they were formed 0.465 grams of tubers. In the third year plants bloom for the first time. The period until flowering in the plant was called maturity period and Dactylorhiza euxina (Nevski) Czerep. was found to have a 3-year maturity period. Plants produced tuber 0.850 g in the third year, 1.585 g in the fourth year and 2.522 g in the fifth year. According to the mathematical modeling, the relationship between year (Y) and fresh tuber weight (FTW) was found Y = 1.22 + (1.61 × FTW) and there was a significant relationship among year, fresh tuber weight, and total biomass (TBM). This equation Y = 1.05 + (0.76 × FTW) + (0.14 × TBM) shows us the mathematical relations of year, fresh tuber weight, and total biomass. The mathematical relations among to fresh tuber weight, total biomass, and plant height (PH) was produced Y = 1.74 - (0.11 × FTW) + (0.57 × TBM) - (0.09 × PH). R2 values were found 0.95-0.99. All R2 values and standard errors were found to be significant at the p < 0.01 level.

Pistacia atlantica Desf. subsp. mutica is widely distributed in the Zagros Mountains in Iran and its fruits are used as food. In the present study, the morphological and pomological variation of naturally grown male and female genotypes of this species was investigated. Considerable variations were observed among male and also among female genotypes studied based on the characters measured. Leaf length ranged from 11.03 to 17.67 cm in male genotypes and also from 11.98 to 16.14 cm in female genotypes. Furthermore, leaf width varied from 7.32 to 13.08 cm in male genotypes and from 9.18 to 13.31 cm in female genotypes. Inflorescence length ranged between 4.62 and 5.83 cm in male genotypes and between 5.05 and 9.04 cm in female genotypes. Also, 100-nut weight varied from 13.74 to 22.81 g with an average of 16.09, while 100-kernel weight ranged from 2.11 to 3.60 g with an average of 2.65 in female genotypes. Leaf length was significantly and positively correlated with leaf width. Furthermore, 100-kernel weight was positively and significantly correlated with leaf and nut dimensions. Principal component analysis showed that the characters measured accounted for 77.41% of the total variance in male genotypes and also 83.24% of the total variance in female genotypes. The genotypes studied showed high variation based on the characters recorded. The wide adaptation of P. atlantica Desf. subsp. mutica highlights its potential as a genetic resource for pistachio breeding for resistance to abiotic and biotic stresses such as drought. The current findings may be useful to select conservation strategies and genetic improvement of the species studied.

Morphological integrations are unknown to forestry seeds. Understanding if seed measurements could predict its future seedlings features is a central question in seedling production.Hymenaea courbarilis an important species in this context and to the applied forestry. Thus, this study aimed to understand how some seedling features, could be related to the seed weight, and be affected by the population origin. The measurements consisted of seedling collar diameter, weight, protophilus area, central and lateral vein. Seed weight consistently varied between the populations in the study. Both populations had higher weight ranges than mentioned in the literature. There was no strong evidence that greater seed weight requires lesser time to germinate, conflicting with previous information. All the measurements presented enough shreds of evidence to be considered different when comparing the populations, except for the protophilus area and lateral vein length. All the studied measurements presented low correlation indexes to seed weight, except for seedling collar diameter, and seedling weight, which presented a moderate correlation. Protophilus elongation pattern was strongly associated with the leaf width when compared to midvein.

portuguesOs objetivos deste trabalho foram verificar se ha variabilidade genetica e avaliar as relacoes lineares entre a arquitetura de planta e a produtividade de graos de milho. Foram conduzidos tres experimentos no delineamento blocos completos ao acaso. Em 51 cultivares, foram avaliados caracteres relacionados ao numero de folhas, as alturas de planta e de espiga, ao ângulo foliar, ao comprimento da folha, a largura da folha, a area foliar e a produtividade de graos, totalizando 22 caracteres. Foram realizadas analises de variância individuais, verificados os pressupostos de normalidade dos erros e homogeneidade de variâncias residuais e agrupadas as medias de cultivares pelo teste de Scott-Knott. Foi determinada a matriz de correlacao fenotipica entre os 22 caracteres das 51 cultivares. Ha variabilidade genetica entre as cultivares em relacao ao numero de folhas, alturas de planta e de espiga, ângulo foliar, comprimento da folha, largura da folha, area foliar e produtividade de graos. Folhas localizadas proximas a espiga apresentam menor ângulo foliar e maiores comprimento da folha, largura da folha e area foliar. O ângulo foliar aumenta gradativamente em direcao as folhas da extremidade inferior e superior da planta. O comprimento da folha, a largura da folha e a area foliar diminuem gradativamente em direcao as folhas da extremidade inferior e superior da planta. Cultivares com maior numero de folhas e maior area foliar estao associadas a plantas com maior produtividade de graos EnglishThe objectives of this study were to analyze whether there is genetic variability and assess the linear relationships between plant architecture and maize grain yield. Three experiments were carried out in a complete randomized block design. A group of 51 cultivars was assessed in relation to 22 traits: number of leaves, plant height, ear height, leaf angle, leaf length, leaf width, leaf area, and grain yield. Individual analyses of variance were performed, the assumptions of normality of errors and homogeneity of residual variances were tested, and means were grouped by the Scott-Knott test. The phenotypic correlation matrix was constructed using the 22 traits of the 51 cultivars. Results showed that there is genetic variability among cultivars for number of leaves, plant height, ear height, leaf angle, leaf length, leaf width, leaf area, and grain yield. Leaves close to the ear have smaller leaf angle and larger length, width, and area of the leaves. Leaf angle gradually increases towards the lower and upper ends of the plant. Length, width, and area gradually decreases in leaves towards the lower and upper ends of the plant. Cultivars with higher number of leaves and larger leaf area are associated with higher grain yield

Although plant organ shapes are defined by spatio-temporal variations of directional tissue expansion, this is a little characterized aspect of organ growth regulation. Although it is well known that the plant hormone gibberellin increases the leaf length/with ratio, its effects on cell expansion in the growing leaf are largely unknown. To understand how variations in rate and anisotropy of growth establish the typical monocotelydonous leaf shape, we studied the leaf growth zone of maize (Zea mays) with a kinematic analysis of cell expansion in the three directions of growth: proximo-distal, medio-lateral, and dorso-ventral. To determine the effect of gibberellin, we compared a gibberellin-deficientdwarf3mutant and the overproducing UBI:GA20OX-1 line with their wild types. We found that, as expected, longitudinal growth was dominant throughout the growth zone. The highest degree of anisotropy occurred in the division zone, where relative growth rates in width and thickness were almost zero. Growth anisotropy was smaller in the elongation zone, due to higher lateral and dorso-ventral growth rates. Growth in all directions stopped at the same position. Gibberellin increased the size of the growth zone and the degree of growth anisotropy by stimulating longitudinal growth rates. Inversely, the duration of expansion was negatively affected, so that mature cell length was unaffected, while width and height of cells were reduced. Our study provides a detailed insight in the dynamics of growth anisotropy in the maize leaf and demonstrates that gibberellin specifically stimulates longitudinal growth rates throughout the growth zone.

This study was conducted to investigate the efficacy of freshwater alga, Chlorella fusca on the improvement of growth and qualities in organic spinach and Chinese chives farm. The average height of Chinese chives treated with the chlorella was 3.7 cm smaller than that of the untreated. The leaf width and fresh weight of Chinese chives treated with the chlorella was 0.5 mm wider and 30.3 g heavier than that of the untreated. The commercialization and yield of Chinese chives treated with the chlorella was 11.9% and 18.3%, respectively higher than that of the untreated. Also, the disease severity of gray mold disease of Chinese chives treated with the chlorella was reduced by more than 24.2% when compared with the untreated. The thickness and number of spinach leaves treated with chlorella was 27.9% and 41.8%, respectively higher than that of the untreated. The fresh weight and yield of the spinach treated with the chlorella was 63.6% and 31.5%, respectively higher than that of the untreated. Moreover, the mineral content of K, Ca, Mg, P, Fe, and Mn were recorded higher in the spinach treated with chlorella compared with that of untreated. The results indicated that the freshwater alga, Chlorella fusca is efficient and economical biostimulant in improving plant growth and quality of Chinese chives and spinach in organic farm.

Oats are widely used in cold regions as forage and grain crop, with early maturing oat varieties having long planting history. More attention is paid to variety maturity, including both naked and hulled types, for production in cold regions. Despite wide planting of early maturing oat in the cold regions in China, no systematic evaluation on yield and quality of the oat varieties in these regions has been conducted. Qinghai and Inner Mongolia are typically cold regions in China due to high elevation and high latitude respectively, and are representative areas for experimentation on growing oats in cold regions. In this study, we tested production and quality of early and late maturing oat varieties at five locations in Qinghai Province and one location in Inner Mongolia Autonomous Regions. We measured several growth, yield and quality parameters of four early maturing and three late maturing oat varieties in the former locations and six early maturing and one late maturing oat varieties at the latter locations. Parameters measured included leaf length, leaf width, panicle length, dry forage yield, seed yield crude protein and others. Attention was primarily paid to oats maturity due to the emphasis on forage and crop productivity without cons derma for to what the varieties were naked and hulled. The results showed that, in the regions of Qinghai Province with high altitude, forage yield and quality parameters of the early maturing varieties were not significantly different from those of the late maturing varieties. However, seed yield of the early maturing varieties (3702 kg/hm2) was significantly higher than that of the late maturing varieties (2759 kg/hm2) ( P

Nowadays people are increasingly concerned about the importance of product quality. The use of fertilizers derived from organic materials is believed to bring more benefits to agricultural products where products become healthier, environmentally friendly and can reduce the negative impact of chemicals that are harmful to humans and the environment. Veg mole is expected in addition to its local microorganisms also Nitrogen element content can increase the growth and production of mustard plants.The purpose of this study is to determine the effect and to get a good dose of MOL vegetable waste on the growth and production of mustard plants.The experiment was conducted experimentally using non factorial completely Randomized Design (RAL) consisting of 5 treatment levels and 4 replications to obtain 20 experimental units. Each plot consists of 16 plants and 4 plants as sample. The level of treatment is as follows: Mo: Without giving MOL of vegetable waste, M1: Giving MOL vegetable waste 75 cc / l water, M2: Giving of MOL vegetable waste 150 cc / l water, M3: Giving MOL vegetable waste 225 cc / l water , M4: Giving MOL vegetable waste 300 cc / l waterThe result of this research can be concluded that vegetable MOL has no significant effect on all observed parameters, ie plant height (cm), number of leaf (strands), leaf length (cm), leaf width (cm), wet weight (g) consumption (g). Keywords: vegetable waste, mustard greens

Twenty four accessions of Plantago ovata were grown to study existing variability, correlation and cause and effect relationships between yield and its component traits with their possible view in future improvement programmes. Analysis of variances displayed highly significant differences among accessions for all the traits. High heritability (>75%) coupled with the high genetic advance (>30%) were observed for the traits viz., leaf width, number of spikes/plant, biological yield/plant and seed yield/plant suggesting predominance of additive gene action. Seed yield/plant exhibited highly significant and positive correlation with biological yield/plant followed by number of spikes/plant, effective tillers/plant, harvest index, seed weight/spike, spike weight and husk recovery both at genotypic and phenotypic level. Biological yield/plant, harvest index, spike weight, effective tillers/plant and husk recovery exhibited the highest positive and significant direct effect on seed yield/plant. Critical analysis of results obtained from character association and path analysis indicated that the traits viz., biological yield/plant, harvest index, spike weight, effective tillers/plant and husk recovery were of prime concern as they possessed high positive association and direct effects on seed yield/plant. These traits are more reliable for selection for yield improvement programmes in Plantago ovata.

This work was carried out at Ghazala Experimental Farm, Fac. Agric., Zagazig Univ., Egypt, during the two successive winter seasons of 2015/2016 and 2016/2017, to estimates the genetic parameters for Balady cabbage, using North Carolina Mating Design II. Ten selected plants were used for this study (five as males and other five as females) from Balady cabbage population. Results of this study showed insignificant mean squares for male × female interaction for all the studied traits, except that for stem diameter, leaf width, wrapper leaves weight per plant and head height, which were highly significant. Estimations of additive variance (σ2A) were higher than dominance variance (σ2D) for stem diameter, leaf width, head height, head diameter and head weight per plant (yield), indicate the important of additive gene action in the inheritance of these traits. Nevertheless, σ2D was higher than σ2A for stem height from wrapper leaves to head, leaf length, and wrapper leaf number and weight per plant, so that the dominance was played very important role in the inheritance of these traits. The heritability in broad-sense ranged from -4.30% for stem height up to wrapper leaves to 83.80% for leaf width. While, narrow-sense of heritability ranged from -2.10% for stem height up to wrapper leaves to 97.35% for head height.

Leaves are a significant component of the shoot system in grasses, functioning in light capture and photosynthesis. Leaf width, length, and angle are expressions of development that collectively define canopy architecture. Thus, the distinctive morphology of grass leaves is an interdependent readout of developmental patterning and growth along the proximal-distal, medial-lateral, and adaxial-abaxial axes. Here, we review the chronology of patterning and growth, namely along the proximal-distal axis, during maize leaf development. We underscore that patterning and growth occur simultaneously, making use of shared developmental gradients and molecular pathways.

Exposure of vegetable seedlings to lowered temperature affects their growth and the content of certain chemical constituents. Chilling activates defensive mechanisms against stress and leads to acclimatization which increases plant ability to withstand harsh field conditions. Thus, seedlings subjected to low-temperature treatment had altered metabolic pathways, and these changes can persist until harvest. We therefore assessed: (i) the direct response of broccoli seedlings to 1 week or 2 weeks at lowered temperatures (6 °C, 10 °C, 14 °C, and 18 °C—control), and (ii) the long-term effects of the latter treatments on phytochemical components level in mature heads of broccoli cultivated in the field. Chilling stress decreased seedling shoot and root fresh and dry weights, plant height, number of leaves, leaf area, leaf perimeter, and leaf width. The most spectacular reductions in these parameters were observed at 6 °C and 10 °C. Longer exposure to lowered temperature resulted in greater reduction in the values of morphological traits. Chilling led to reduction in L-ascorbic acid content in broccoli seedlings, while a 6 °C temperature caused an increase in soluble sugars. The highest content of dry weight, soluble sugars, and L-ascorbic acid were observed in the heads of plants exposed to 14 °C, however, the content of dry weight (at 10 °C) and L-ascorbic acid (at 6 °C and 10 °C) also increased in broccoli heads in comparison with the control. Longer chilling (for 2 weeks) generally increased the content of these constituents in mature broccoli. Lower temperature (6–14 °C) applied at seedling stage increased P and Zn contents in broccoli heads in comparison to the control, whereas plants treated with 10 °C had more K, Fe, and, together with 6 °C treatment, Cu. The lowest temperature applied to the seedlings (6 °C) caused an increase in Mn content, while no effects of seedling chilling was noted for Ca levels. Significant linear correlations were noted and regression models were developed for the content of dry weight, soluble sugars, and L-ascorbic acid in the broccoli heads based on the chosen seedling parameters. The results show that the effect of lowered temperature to which the seedlings were subjected persists also in the further stages of plant ontogenesis, causing permanent changes in the chemical composition of mature broccoli heads.

Tomato is often exposed to diverse abiotic stresses and cold stress is one of harsh environmental stresses. Abnormal low temperature affects tomato growth and development, leading to, e.g., physiological disorders, flower drops, and abnormal fruit morphology, and causing a decrease in tomato yield and fruit quality. It is important to identify low temperature-(LT) tolerant tomato (Solanum lycopersicum L.) cultivars relying on different fruit types. In this study, our focus was to analyze the physiological traits of 35 tomato accessions with three different fruit types (cherry, medium, and large sizes) under night temperature set-points of 15 °C for control temperature (CT) and 10 °C for LT, respectively. Plant heights (PH) of most tomato accessions in LT were remarkably decreased compared to those in CT. The leaf length (LL) and leaf width (LW) were reduced depending on the genotypes under LT. In addition, the number of fruits (NFR), fruit set (FS), fruit yield (FY), and marketable yield (MY) were negatively affected in LT. The variation was further investigated by the correlation, the principal component (PCA), and the cluster analysis. Interestingly, positive correlations between different vegetative and reproductive traits were uncovered. Multivariate analysis including the PCA and hierarchical clustering classified the LT-treated 35 tomato accessions into four major groups. The identified accessions were associated with vegetative and reproductive parameters on positive directions. The results might be utilized for establishing breeding programs on selecting LT-tolerant tomato cultivars with different selection indices relying on fruit types during vegetative and/or reproductive stages.

The nature and the magnitude of genetic divergence were estimated in 54 rice genotypes collected from Arunachal Pradesh and upper parts of Assam state using Mahalonobis’s D 2 statistics. The analysis of variance revealed significant differences among the genotypes for nine quantitative characters studied. The genotypes were grouped into 18 clusters showing fair degree of relationship between geographic distribution and genetic divergence. All the minimum and maximum cluster mean values were distributed in relatively distant clusters. Maximum numbers of genotypes were included in cluster I which has 26 genotypes. Traits contributing maximum to genetic divergence viz ., plant height, days to 50% flowering, 100 grain weight and leaf width may be utilized in selecting genetically diverse parents.

The fig tree is native to Iran, east of Asia and Syria. Shortage of precipitation and drought is one of the greatest problems in rain-fed fig cultivation areas. A random complete block design with three replications was used to investigate the effect of trunk thinning on drought disorders. Since 'Sabz' fig trees are trained with multiple trunks, 16 intensities of trunk thinning applied during winter pruning were compared. The treatments were obtained pruning five different groups of tree to leave 0, 1, 2, 3, or 4 trunks tree-1, respectively. The pruning intensity of each of the 16 treatments was evaluated calculating the ratio between the total circumference of the remaining trunks and the total trunk circumference before the application of thinning treatment (here after trunk diameter ratio). The results suggested that the different levels of trunk thinning had positive effects on leaf width, leaf number, leaf water potential, leaf temperature, fig fruit color and size. Thinning all the trunks had the best effect on tree vegetative appearance under drought stress conditions. Trunk thinning treatment that pruned trees to leave 2 trunks and provided a trunk diameter ratio of 0.5 was more suitable than other treatments in terms of vegetative growth and fruit quality and quantity. The latter pruning strategy is recommended for increasing tree tolerance to drought, fruit yield and quality. This will increase grower income and allow the protection of rain-fed fig orchards in dry years.

This study aims to determine the differences in leaf functional traits and phenotypic plasticity of leaf functional traits between exotic and native Compositae plant species. Leaf width of exotic plants was significantly lower than that of native species. Leaf length, specific leaf area (SLA), single-leaf wet and dry weights, leaf moisture, and leaf thickness of exotic plants were also lower than those of native species but not significantly. The leaf shape index of exotic plants was higher than that of native species but not significantly. This implies that the relatively low leaf construction cost for exotic plants may play an important role in the success of their invasions. The higher leaf shape index and lower leaf width of exotic plants can enhance the efficiency of resource capture (especially sunlight capture) via adjustments to leaf shape and size, thereby increasing the survival of exotic plants. The plasticity indices of single-leaf wet weight and leaf thickness of exotic plants were significantly lower than those of native species. The lower phenotypic plasticity of single-leaf wet weight and leaf thickness of exotic plants may be the result of a cost to plasticity. That is, if the plasticity is too high, the fitness of plant species might be reduced sharply under unfavorable environments. Thus, lower plasticity of leaf functional traits may compensate for the negative impact of adverse environments and stabilize leaf construction costs for exotic plants. Moreover, reduced phenotypic plasticity might be one of the key competitive strategies by which exotic plants successfully invade new habitats. Overall, exotic plants did not always exhibit higher values of leaf functional traits or increased phenotypic plasticity of leaf functional traits compared with native species.

Introduction: Spinach is an important leafy vegetable in the cold season, and despite the fact that is considered as low-calorie food source contains significant amount of minerals such as iron, and vitamin A and C. According to the University of Utah 3.8 dS m-1 is salinity tolerance threshold for the spinach and yield decrease that have been reported by 10%, 25% and 50% at 5.5, 7 and 8 dS m-1 salinity. The necessity to supply adequate potassium has been demonstrated in salinity conditions. In salt stress conditions, foliar application of K in spinach, reduces the harmful effects of salt and increase the ratio of potassium to sodium (1.61 to 2.72). Foliar application of K with prevent of potassium transfer from root to shoot is causing continuation of photosynthesis and reduce the effects of salinity. Absorption of potassium from the leaves depends on the type of used compound. In this context, characteristics of plant (leaves with a waxy composition, duration of growth and leaf area) are important. 100 kg ha-1 of potassium in salt stress conditions by reducing the absorption of sodium, increased salt tolerance on the sunflower. Materials and Methods: In order to evaluate the foliar application of K on the yield and yield components of spinach in salt stress condition, a study was conducted in 2012 by using split plot randomized based on complete block design with four replications at Isfahan Agricultural and Natural Resources Research Station. Three levels of irrigation water salinity consisted of a control (2 dS m-1), well water with salinity (4 dS m-1) and well water with salinity (8dS m-1) arranged in main plots and two levels of control and foliar applications of potassium fertilizer containing potassium oxide solubility in water (2.5 ml per liter) arranged in subplots. Statistical analysis was conducted by using SAS software and statistical tests were compared with Duncan at 5 percent. Result and Discussions: The results showed that the yield of spinach with foliar application of K and 4 dS m-1 salinity was equaled 35300 kg ha-1 which had not significantly different from control treatment. Foliar application of K and 8 dS m-1 salinity, and also 8 dS m-1 salinity and without foliar potassium, had 20.2 and 38% yield reduction, respectively. In salinity 2.1, 4 and 8 dS m-1 plants m-2 were 40, 38.1 and 29.1, respectively. Leaf dry matter percent was improved with foliar application of K in 8 dS m-1 salinity. Effect of potassium, as modulators of salt in spinach, by researches of Shannon and Greve (24) and Kaya et al (14) have also been emphasized. Spinach leaves number was from 11.4 to 16.7 in different treatments. Foliar application of K in 4 and 8 dS m-1 salt treatment was increase in the number of leaves. This increased in treatment of 4 and 8 dS m-1 was 15.3 and 28.9 percent, respectively. In both saline treatments of 4 and 8 dS m-1, leaf length was positively affected by the application of potassium but in salinity 4 dS m-1 (unlike the eight salinity dS m-1) leaf width was not affected by the potassium spraying. The ability of plants to maintain intracellular potassium to sodium ratio leaves in certain extent is necessary for a salt tolerance. In fact, the application of potassium in salinity conditions by increasing the concentration of the K in organs is a kind of acclimation to the salt stress and activates repair mechanisms of the damage against of stress agent. The length of tail leaves in 4 dS m-1 salt was not significantly affected by the spraying of potassium while in 8 dS m-1 salinity, spraying potassium led to an increase of 28 percent in length of leaf tail. The effect of K application on the dry matter content in the 8 dS m-1 salinity was statistically significant. Potassium is the most abundant cation-forming in many plants (typically more than 10% of dry weight) and less than 10 grams per kg-1 of dry weight appear deficiency symptoms in most plants. Conclusion: According to the results, in salinity conditions, the foliar application of K can be used as a factor to reduce the harmful effects of salinity. While the lack of fresh water for irrigation in arid and semi-arid crops has become a challenge, attention to the moderated mechanisms of the harmful effects of salt, is one of the approaches to face the challenges ahead.

Allium cepa, an Amaryllidaceous member traditionally used as anti-thrombotic, anti-platelet, anti-asthmatic, anti-biotic, anti-carcinogenic and anti-blood clotting. It also improves sleep quality, circulation, skin and hair maintenance, hair growth stimulant, heart ailments, blood pressure, lowers cholesterol. Study was aimed at growth dynamics of Onion in two districts of Gilgit-Baltistan i.e. Hunza and Gilgit. Research revealed that mean leaf length (cm), width (cm) and area (cm2) of plant collected from Hunza were 14.88 cm, 0.43 cm and 9.0 cm2 respectively. In district Hunza leaf length grows 97.1 percent faster as compared to its growth in width and width increase is only 2.9% to the increase in leaf length. Similarly, plants collected from district Gilgit showed a mean values of leaf length, width and area as 25.05 cm, 0.59 cm and 10.1 cm2 respectively. Average growth of leaf length in Gilgit is 97.64 percent (%) faster than its growth in width which is only 2.35%. this shows that growth in district Gilgit is relatively higher (12.70%) than in Hunza. Roots arising from the bulb have an average length of 0.40 cm in Hunza as compared to 0.44 cm in Gilgit. This shows minute difference between the two and samples from Gilgit show around 10% long. Adventitious roots grow almost 97.3% slow as compared to its leaves above the ground. Mean weight of bulbs collected from district Hunza was 9.62g as compared to average 16.25g in Gilgit. Bulb growth in district Gilgit is almost 60% (59.2%) faster as compared to Hunza leading to bigger and heavier bulbs in Gilgit.? Mean values of Onion populations from both the districts show leaf length (19.97cm), leaf width (0.506cm), leaf area (9.55 cm2), root length (0.42) and weight (12.936).

The growth-survival trade-off hypothesis states that functional traits of stem, wood, leaves and fruits govern acquisitive and conservative strategies of plant species that grow/survive differently in forests. We aimed to determine whether heterogeneity during the colonization of a secondary Atlantic Forest fragment causes differences in colonization-related traits and determines taxonomic composition of subplots. A total of 5,078 subplots were censused with leaf and fruit traits and height measurements being determined for each tree of each species, and with averaged-abundance-weighted values calculated for each subplot. A dendrogram was generated from the taxonomic composition of subplots using Sorensen similarity and UPGMA clustering. A total of 74,335 trees of 178 species were recorded. Clustering revealed five main groups of subplots that were tested against each other and found to differ in terms of average leaf width, leaf-width/petiole-width, wood density, mean height, height mode, fruit width and fruit length. Greater leaf averages in subplots were associated with greater fruit averages, and with higher wood densities and greater heights among groups of subplots, revealing that heterogeneity during colonization directed the communities of the 50-ha plot. The averaged values of functional traits detected subplots associated with conditions favorable to colonization, thereby reinforcing the growth-survival trade-off hypothesis.

In barley (Hordeum vulgareL.), lateral branches called tillers contribute to grain yield and define shoot architecture, but genetic control of tiller number and developmental rate are not well characterized. The primary objectives of this work were to examine relationships between tiller number and other agronomic and morphological traits and identify natural genetic variation associated with tiller number and rate, and related traits. We grew 768 lines from the USDA National Small Grain Core Collection in the field and collected data over two years for tiller number and rate, and agronomic and morphological traits. Our results confirmed that spike row-type and days to heading are correlated with tiller number, and as much as 28% of tiller number variance is attributed to these traits. In addition, negative correlations between tiller number and leaf width and stem diameter were observed, indicating trade-offs between tiller development and other vegetative growth. Thirty-three quantitative trait loci (QTL) were associated with tiller number or rate. Of these, 40% overlapped QTL associated with days to heading and 22% overlapped QTL associated with spike row-type, further supporting that tiller development is influenced by these traits. Despite this, some QTL associated with tiller number or rate, including the major QTL on chromosome 3H, were not associated with any other traits, suggesting that tiller number can be modified independently of other important agronomic traits. These results enhance our knowledge of the genetic control of tiller development in barley, which is important for optimizing tiller number and rate for yield improvement.

Cytokinins are physiologically active adenine derivatives that are vital to the regulation of various de velopmental processes in plants, such as vegetative growth and flower induction. The objective of this study was to understand how the application of cytokinin trans-zeatin riboside affects growth in ba nana (Musa AAA Simmonds) cv. Williams plants during the vegetative phase on two farms located in the production zone of the Magdalena province, Colombia. The effect of trans-zeatin riboside applica tions at doses of 0.00, 0.05, 0.25, or 0.45 mg L-1 via foliar spraying was evaluated, with 15 days between the applications, starting with transplant to the field and lasting until 10 weeks of growth. The treat ment 0.05 mg L-1 of cytokinin resulted in increases in the plant growth variables, such as height in V, pseudostem diameter, leaf width, and leaf area. RESUMEN Las citoquininas son compuestos fisiológicamente activos derivados de adenina, vitales para la regulación de varios procesos de desarrollo en plantas, tal como el crecimiento vegetativo y la inducción floral. El objetivo de este trabajo fue conocer como la aplicación de citoquinina trans-zeatina ribósido afecta el crecimiento vegetativo de plantas de banano (Musa AAA Simmonds) cv. Williams en dos fincas ubicadas en la zona bananera del departamento de Mag dalena, Colombia. Se evaluó el efecto de la aplicación de trans-zeatina ribósido en dosis de 0,00; 0,05; 0,25 o 0,45 mg L-1 vía foliar, con espacio entre aplicación de 15 días iniciando en el momento de trasplante de plantas al sitio definitivo y hasta 10 semanas de crecimiento. El tratamiento de plantas con 0,05 mg L-1 de citoquinina resultó en incrementos en variables del crecimiento, tales como altura a la V, diámetro del seudotallo, ancho foliar y área foliar.

Faot MM, Zubaidah S, Kuswantoro H. 2019. Genetic correlation and path analysis of agronomical traits of soybean (Glycine max) lines infected by CpMMV. Biodiversitas 20: 1496-1503. CpMMV is a virus that can decrease soybean production. The virus vector is an insect where the control is carried out using a chemical insecticide that is less environmentally friendly. Developing a superior variety of soybean that is resistant to CpMMV is one of the solutions to solve such a problem. This research aimed to study the relationship of agronomical traits to the yield of soybean line infected by CpMMV. Ten lines and two varieties of soybean with four replications was used in this study in a randomized complete block design. Bemisia tabaci was used in CpMMV infestation as the vector of the disease. Rearing Bemisia tabaci was done forty days before sowing the main experiment, and it placed surrounding the main experiment plots. The observation variables were disease severity, days to flowering and maturity, plant height, length, width and ratio of the leaf, number of branches and reproductive nodes, number of filled and unfilled pods, number of seeds, 100 seeds weight, and seed yield per plant. The data was analyzed for genotypic and phenotypic correlation, and the path analysis for direct and indirect effects of disease severity, and agronomical characters. The results showed that CpMMV infestation caused disease severity by about 20-28.5%. A significant positive phenotypic correlation to the seed yield per plant was shown by the number of reproductive nodes and the 100 seeds weight. Meanwhile, a significant positive genotypic correlation to the seed yield per plant was shown by the days to maturity and the number of branches. The number of reproductive nodes, the 100 seeds weight, and the number of seeds per plant showed a high direct effect to the seed yield per plant. The high positive indirect effect was also shown by the number of filled pods, days to flowering, plant height, leaf length, leaf width, the number of reproductive nodes, and the number of branches through the number of seeds per plant was also. Therefore, the selection criteria for high yielding CpMMV-resistant soybean lines can be based on the number of reproductive nodes and 100 seeds weight.

Leaf blade width, curvature, and cuticular wax are important agronomic traits of rice. Here, we report the rice Oschr4-5 mutant characterized by pleiotropic phenotypes, including narrow and rolled leaves, enhanced cuticular wax deposition and reduced plant height and tiller number. The reduced leaf width is caused by a reduced number of longitudinal veins and increased auxin content. The cuticular wax content was significantly higher in the Oschr4-5 mutant, resulting in reduced water loss rate and enhanced drought tolerance. Molecular characterization reveals that a single-base deletion results in a frame-shift mutation from the second chromodomain of OsCHR4, a CHD3 (chromodomain helicase DNA-binding) family chromatin remodeler, in the Oschr4-5 mutant. Expressions of seven wax biosynthesis genes (GL1-4, WSL4, OsCER7, LACS2, LACS7, ROC4 and BDG) and four auxin biosynthesis genes (YUC2, YUC3, YUC5 and YUC6) was up-regulated in the Oschr4-5 mutant. Chromatin immunoprecipitation assays revealed that the transcriptionally active histone modification H3K4me3 was increased, whereas the repressive H3K27me3 was reduced in the upregulated genes in the Oschr4-5 mutant. Therefore, OsCHR4 regulates leaf morphogenesis and cuticle wax formation by epigenetic modulation of auxin and wax biosynthetic genes expression.

Kalimantan whose existence is increasingly extinct so it needs to be preserved by in vitro propagation. This study aims to determine the effect of a combination of the growth regulators, Benzyl Amino Purin (BAP), Kinetin, Naphthalene Acetic Acid (NAA) and banana extract on the growth of sugar cane orchids in vitro. The basic media used were Half Murashige and Skoog (1/2 MS), on the base media were added 30 g.L-1 sugar, 8 g.L-1 seaweed commercial powder, plus BAP 3 ppm, Kinetin 3 ppm, NAA 0.5 ppm, and ambon banana extract 100 g.L-1, the acidity (pH) of the media is adjusted to around 5.8. The research design used was a Completely Randomized Design (CRD), each treatment was repeated six times. Data were analyzed using variance analysis and continued with DMRT (Duncan Multiple Range Test) tests with a level of 5%. The results showed that the half media Murashige and Skoog was good for the propagation of sugarcane orchids. The addition of BAP growth regulators, Kinetin, NAA, and banana extracts showed a good response to growth culture. The addition of BAP 3 ppm + NAA 0.5 ppm showed the best results for the height increased, leaf number increased, leaf length increased, leaf width increased, and the number of shots increased. The addition of Ambon banana extract to media containing growth regulators tended to decrease the growth response, seen in the parameters of plant height increased, leaf number increased, leaf length increased, leaf width increased, the number of shoots increased, the number of roots increased and in root length increased.