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1. Fusion of protoplasts with irradiated microprotoplasts as a tool for radiation hybrid panel in citrus

Author

Bona,Claudine Maria de, Stelly,David, Miller Jr.,J. Creighton, and Louzada,Eliezer Silva

Subjects
gamma rays, Citrus, Swinglea glutinosa, asymmetric somatic hybridization, fungi, radiation hybrid mapping, food and beverages, hybrid cell lines
Abstract

The objective of this work was to combine asymmetric somatic hybridization (donor-recipient fusion or gamma fusion) to microprotoplast-mediated chromosome transfer, as a tool to be used for chromosome mapping in Citrus. Swinglea glutinosa microprotoplasts were irradiated either with 50, 70, 100 or 200 gamma rays and fused to cv. Ruby Red grapefruit or Murcott tangor protoplasts. Cell colonies were successfully formed and AFLP analyses confirmed presence of S. glutinosa in both 'Murcott' tangor and 'Ruby Red' grapefruit genomes.

Published
2009

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