Search

Your search keyword '"Ganbaatar, Jamsranjav"' showing total 2 results
Start Over Author "Ganbaatar, Jamsranjav" Topic fomitopsis officinalis
2 results on '"Ganbaatar, Jamsranjav"'

1. Structure characterization of the mannofucogalactan isolated from fruit bodies of Quinine conk Fomitopsis officinalis

Author

Naranmandakh Shinen, Victoria V. Golovchenko, Ganbaatar Jamsranjav, Alexander O. Chizhov, Alexander S. Shashkov, and Daria S. Khramova

Subjects
chemistry.chemical_classification, Chromatography, Polymers and Plastics, 010405 organic chemistry, Organic Chemistry, Extraction (chemistry), Size-exclusion chromatography, Fomitopsis officinalis, 02 engineering and technology, Nuclear magnetic resonance spectroscopy, 021001 nanoscience & nanotechnology, Polysaccharide, 01 natural sciences, 0104 chemical sciences, chemistry.chemical_compound, Residue (chemistry), chemistry, Materials Chemistry, Side chain, Medicinal fungi, 0210 nano-technology
Abstract

The mannofucogalactan as a major component of water extract was obtained from fruit bodies of Fomitopsis officinalis by extraction with boiling water followed by deproteination, decoloration, and purification using anion-exchange chromatography and size exclusion chromatography. Its structure was characterized using the data of monosaccharide composition, methylation analysis, one- and two-dimensional NMR spectroscopy. The studied polysaccharide was a branched mannofucogalactan with a backbone composed of partially 3-O-methylated 1,6-O-linked α- D -galactopyranosyl residues. Almost every second residue in the backbone was substituted at O-2 by 3-O-α- D -mannopyranosyl-α- L -fucopyranosyl and β- D -galactopyranosyl residues. The non-reducing terminal α- L -fucopyranosyl units, which were identified by GC–MS analyses, appeared to be the part of mannofucogalactan side chains also.

Published
2018

2. Structural investigation and comparative cytotoxic activity of water-soluble polysaccharides from fruit bodies of the medicinal fungus quinine conk.

Author

Golovchenko, Victoria V., Naranmandakh, Shinen, Ganbaatar, Jamsranjav, Prilepskii, Artur Yu., Burygin, Gennady L., Chizhov, Alexander O., and Shashkov, Alexander S.

Subjects
*FRUITING bodies (Fungi), *BETA-glucans, *POLYSACCHARIDES, *QUININE, *CONGO red (Staining dye), *CHINESE medicine, *APOPTOTIC bodies
Abstract

The structures and cytotoxic activities of water-soluble polysaccharides were investigated to search for biologically active polysaccharides from the fruit bodies of quinine conks (Fomitopsis officinalis). The decoctions of this medical fungus are actively used in folk medicine in many countries and traditional Chinese medicine. From the fungal extract we prepared, only branched β-glucan had cytotoxic activity among all the water-soluble polysaccharides. This glucan is characterized by a regular structure. Its backbone is formed by 1,3-linked β-D-Glc p residues, of which every third residue is substituted at O-6 by a single β-D-Glc p residue. It has a triple helix conformation according to the data obtained from a colorimetric assay with Congo red dye and is characterized by a high-weight average molar mass (Mw > 800 kDa). β-Glucan possessed cytotoxic activity against HeLa cells (IC 50 = 318 ± 47 μg/mL) and induced the formation of apoptotic bodies around most cancer cells at a concentration of 200 μg/mL. It should be noted that extraction with boiling water, which is usually used to obtain extracts and decoctions, is unable to isolate active β-glucan. Active β-glucan can be obtained in an individual state by cold alkali extraction after dehydration of the fruit bodies and removal of the components extractable by boiling water. Image 1 [ABSTRACT FROM AUTHOR]

Published
2020
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results