1. Syndecan-1 controls cell migration by activating Rap1 to regulate focal adhesion disassembly.
- Author
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Altemeier WA, Schlesinger SY, Buell CA, Parks WC, and Chen P
- Subjects
- Cell Line, Enzyme Activation, Fluorescence Recovery After Photobleaching, Humans, Kinetics, Microtubules metabolism, Nocodazole pharmacology, Paxillin metabolism, Protein Multimerization, Time-Lapse Imaging, Tubulin Modulators pharmacology, rap1 GTP-Binding Proteins, Cell Movement, Focal Adhesions metabolism, Syndecan-1 metabolism
- Abstract
After injury, residual epithelial cells coordinate contextual clues from cell-cell and cell-matrix interactions to polarize and migrate over the wound bed. Protrusion formation, cell body translocation and rear retraction is a repetitive process that allows the cell to move across the substratum. Fundamental to this process is the assembly and disassembly of focal adhesions that facilitate cell adhesion and protrusion formation. Here, we identified syndecan-1 as a regulator of focal adhesion disassembly in migrating lung epithelial cells. Syndecan-1 altered the dynamic exchange of adhesion complex proteins, which in turn regulates migration speed. Moreover, we provide evidence that syndecan-1 controls this entire process through Rap1. Thus, syndecan-1 restrains migration in lung epithelium by activating Rap1 to slow focal adhesion disassembly.
- Published
- 2012
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