1. Click and release: fluoride cleavable linker for mild bioorthogonal separation.
- Author
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Schneider EM, Zeltner M, Zlateski V, Grass RN, and Stark WJ
- Subjects
- Animals, Azides chemistry, Cattle, Chymotrypsin chemistry, Chymotrypsin metabolism, Fluorescent Dyes chemistry, Magnetite Nanoparticles chemistry, Silanes chemistry, Water chemistry, Azides isolation & purification, Chymotrypsin isolation & purification, Click Chemistry, Fluorescent Dyes isolation & purification, Fluorides chemistry
- Abstract
Herein, we present a water dispersable, magnetic nanoparticle supported "click and release" system. The cleavable linker has been synthesized by using a strain-promoted copper-free "click" reagent to establish the specific link and a fluoride cleavable silane moiety for mild cleavage. Small organic molecules, azide-bearing dyes and functionalized enzymes have been bound to the magnetic particle and released in a bioorthogonal way.
- Published
- 2016
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