1. Visualizing Microglia with a Fluorescence Turn‐On Ugt1a7c Substrate.
- Author
-
Kim, Beomsue, Fukuda, Masahiro, Lee, Jung‐Yeol, Su, Dongdong, Sanu, Srikanta, Silvin, Aymeric, Khoo, Audrey T. T., Kwon, Taejoon, Liu, Xiao, Chi, Weijie, Liu, Xiaogang, Choi, Sejong, Wan, Diana S. Y., Park, Sung‐Jin, Kim, Jin‐Soo, Ginhoux, Florent, Je, H. Shawn, and Chang, Young‐Tae
- Subjects
- *
MICROGLIA , *FLUORESCENCE , *STRUCTURE-activity relationships , *TRANSGENIC mice , *NEURAL development , *GLUCURONIDATION - Abstract
Microglia, the brain‐resident macrophage, are involved in brain development and contribute to the progression of neural disorders. Despite the importance of microglia, imaging of live microglia at a cellular resolution has been limited to transgenic mice. Efforts have therefore been dedicated to developing new methods for microglia detection and imaging. Using a thorough structure–activity relationships study, we developed CDr20, a high‐performance fluorogenic chemical probe that enables the visualization of microglia both in vitro and in vivo. Using a genome‐scale CRISPR‐Cas9 knockout screen, the UDP‐glucuronosyltransferase Ugt1a7c was identified as the target of CDr20. The glucuronidation of CDr20 by Ugt1a7c in microglia produces fluorescence. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF