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Your search keyword '"Chu, Hanqi"' showing total 3 results
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3 results on '"Chu, Hanqi"'

1. Downregulation of Cav1.3 calcium channel expression in the cochlea is associated with age-related hearing loss in C57BL/6J mice

Author

Liangqiang Zhou, Xiaowen Huang, Yang Yu, Qingguo Chen, Yun Liu, Yonghua Cui, Jin Chen, Dan Bing, Hao Xiong, Chu Hanqi, and Shaoli Wang

Subjects
medicine.medical_specialty, Calcium Channels, L-Type, Hearing loss, Blotting, Western, Down-Regulation, Fluorescent Antibody Technique, Real-Time Polymerase Chain Reaction, Cav1.3, Mice, Internal medicine, Evoked Potentials, Auditory, Brain Stem, otorhinolaryngologic diseases, medicine, Animals, RNA, Messenger, Organ of Corti, Cochlea, Hair Cells, Auditory, Inner, Absolute threshold of hearing, Voltage-dependent calcium channel, biology, Chemistry, General Neuroscience, Calcium channel, Stria Vascularis, Auditory Threshold, Immunohistochemistry, Human morbidity, Mice, Inbred C57BL, Hair Cells, Auditory, Outer, Auditory brainstem response, Endocrinology, biology.protein, Osteoporosis, medicine.symptom
Abstract

Age-related hearing loss (ARHL) is the most common human morbidity. However, the molecular mechanisms underlying ARHL are little known. In the present study, the expression of Ca(v)1.3 calcium channels in the C57BL/6J ARHL mouse cochlea was investigated. The hearing threshold was assessed by auditory brainstem response and the expressions of Ca(v)1.3.3 calcium channels at the protein and mRNA levels were detected by immunohistochemistry, western blot, and real-time RT-PCR. Associated with the auditory brainstem response threshold increased with age, the Ca(v)1.3.3 expression was gradually decreased. In comparison with 4-week-old mice, Ca(v)1.3.3 expressions in the cochlea at 14, 24, and 48 weeks of age were significantly and gradually decreased at both the protein and the mRNA levels. Immunohistochemistry showed that the expression of Ca(v)1.3.3 was apparently reduced at the inner hair cells, outer hair cells, and stria vascularis in the cochlear lateral wall in the aged mice. Our findings indicate that Ca(v)1.3.3 calcium channel expression in the cochlea is reduced in the ARHL mice and is associated with ARHL. The data also support a view that Ca(v)1.3.3 calcium channel is a good target for prevention and therapy of ARHL.

Published
2013

2. Down-regulation of the large conductance Ca -activated K channel expression in C57BL/6J cochlea.

Author

Pan, Chunchen, Chu, Hanqi, Lai, Yanbing, Liu, Yun, Sun, Yanbo, Du, Zhihui, Chen, Jin, Tong, Ting, Chen, Qingguo, Zhou, Liangqiang, Bing, Dan, and Tao, Yanling

Subjects
*AGE distribution, *ANIMAL experimentation, *CALCIUM, *COCHLEA, *FLUORESCENT antibody technique, *GENE expression, *MICE, *PAIRED comparisons (Mathematics), *POLYMERASE chain reaction, *PRESBYCUSIS, *RESEARCH funding, *WESTERN immunoblotting, *REVERSE transcriptase polymerase chain reaction, *DATA analysis software, *DESCRIPTIVE statistics, *IN vitro studies, *ONE-way analysis of variance, *OLD age
Abstract

Conclusion: The large conductance Ca2+-activated K+ channels (BK) expression is decreased in the cochleae of age-related hearing loss (AHL) mice. BK channel may be associated with AHL. Objective: AHL is the most common among elderly persons. BK channels act as sensors for membrane voltage and intracellular Ca2+ and are essential for hearing. To investigate the distribution of BK channel in the cochleae of C57BL/6J mice, and the relationship between the expression of BK channel and the etiology of AHL. Methods: BK expression was studied in the cochleae of C57BL/6J mice at various ages (4, 12, 26, 52 weeks). The expressions of BK at the protein and mRNA levels were detected by immunofluorescence technique, western blot and quantitative real time PCR. Results: In comparison with 4-week-old mice, BK expressions in the cochleae at 12, 26 and 52 weeks of age were significantly and gradually decreased at both the protein and the mRNA levels. The immunofluorescence technique showed the BK channel was located in the hair cells and cells of the spiral ganglion, spiral ligament and stria vascularis and its expression also decreased with aging. [ABSTRACT FROM AUTHOR]

Published
2016
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3. Age-related change in the expression of NKCC1 in the cochlear lateral wall of C57BL/6J mice.

Author

Liu, Yun, Chu, Hanqi, Chen, Jin, Zhou, Liangqiang, Chen, Qingguo, Yu, Yang, Wu, Zhengong, Wang, Shaoli, Lai, Yanbing, Pan, Chunchen, and Cui, Yonghua

Subjects
*ANALYSIS of variance, *ANIMAL experimentation, *CHLORIDES, *COCHLEA, *FLUORESCENT antibody technique, *MICE, *POLYMERASE chain reaction, *POTASSIUM, *PRESBYCUSIS, *RESEARCH funding, *RNA, *SODIUM, *T-test (Statistics), *WESTERN immunoblotting, *DATA analysis software, *ION transport (Biology), *DESCRIPTIVE statistics
Abstract

Conclusions: Na+-K+-2Cl- co-transporter isoform 1 (NKCC1) mRNA and protein decrease with increasing age in the cochlear lateral wall of C57BL/6J (C57) mice. The down-regulation of NKCC1 may influence the K+ transport efficiency and the homeostasis of ion transport cells, and cause the irreversible damage of cochlear cells in old C57 mice. Our results indicate that NKCC1 may play an important role in the pathogenesis of age-related hearing loss (AHL). Objectives: The aim of the present study was to investigate the relationship between the functional expression of NKCC1 transporter and the etiology of AHL. Methods: C57 mice were used and randomly divided into four groups according to age (4 weeks, 14 weeks, 26 weeks, and 52 weeks). Immunofluorescence technique, quantitative real-time PCR, and western blot were applied to detect the expression of NKCC1 in the cochlear lateral wall of C57 mice at the various ages. Results: In all four groups, the expression of NKCC1 was observed in the stria vascularis and type II fibrocytes of the spiral ligament. Also, the expression of NKCC1 appeared to decrease with age at both the transcriptional level and the protein level. [ABSTRACT FROM AUTHOR]

Published
2014
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