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Your search keyword '"Borisova, Ekaterina"' showing total 4 results
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4 results on '"Borisova, Ekaterina"'

1. Laser-induced autofluorescence study of caries model in vitro.

Author

Borisova E, Uzunov T, and Avramov L

Subjects
Humans, In Vitro Techniques, Lactobacillus, Models, Biological, Staphylococcus aureus, Viridans Streptococci, Dental Caries diagnosis, Dental Caries microbiology, Fluorescence, Low-Level Light Therapy
Abstract

Laser-induced autofluorescence spectra of teeth irradiated by a 337 nm nitrogen laser were measured during in vitro caries formation through initial enamel demineralization and introducing of carious bacterial flora in the lesions developed. Spectra obtained from sound teeth consist of an intensive maximum at 480-500 nm and secondary maximum at 430-450 nm. In the process of caries formation, we observed an increase in the intensity at 430-450 nm and the appearance of two maxima in the red spectral region-at 590-650 nm. The intensity increase at 430-450 nm was related to the tooth demineralization. Bacteria presence and their metabolism products induced an increase in the absorption in the UV-blue spectral region at 350-420 nm and the appearance of a fluorescence signal in the long-wave spectral region at 590-650 nm. From the point of view of tissue optics, these results allow caries to be considered as consisting of two different phenomena-tissue destruction and bacterial flora and its metabolism products increase. The results could be used to obtain a more complete picture of caries formation on the base of its fluorescent properties.

Published
2006
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3. Malignant Tissue Optical Properties

Author

Bashkatov, Alexey N., Zakharov, Valery P., Bucharskaya, Alla B., Borisova, Ekaterina G., Khristoforova, Yulia A., Genina, Elina A., Tuchin, Valery V., Tuchin, Valery V., editor, Popp, Jürgen, editor, and Zakharov, Valery, editor

Published
2020
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4. Macroscopic and microscopic fluorescence spectroscopy of colorectal benign and malignant lesions - diagnostically important features

Author

Ivan Terziev, Lachezar A. Avramov, Maria V. Lomova, Daniil N. Bratashov, Oksana V. Semyachkina-Glushkovskaya, Borislav Vladimirov, Ts. Genova, Ekaterina Borisova, and Borisova, Ekaterina

Subjects
0303 health sciences, Pathology, medicine.medical_specialty, Chemistry, Confocal, Histology, 01 natural sciences, Fluorescence, Article, Atomic and Molecular Physics, and Optics, Fluorescence spectroscopy, Spectral imaging, 010309 optics, 03 medical and health sciences, colon cancer, 0103 physical sciences, Fluorescence microscope, medicine, confocal fluorescence microscopy, fluorescence, Spectroscopy, Ex vivo, 030304 developmental biology, Biotechnology
Abstract

Fluorescence spectroscopy is a sensitive, fast and non-invasive tool for a diagnostics of cancerous gastrointestinal lesions. It could be applied for in situ detection of tumours during primary endoscopic observations or as add-on measurement modality during microscopic observations of tissue histology slides for their initial or retrospective diagnosis. Therefore, we are looking for diagnostically important features of normal and cancerous tissue areas in a broad spectral range for gastrointestinal tissues ex vivo using two steady-state macroscopic fluorescent spectroscopic modalities and by confocal fluorescent microscopic detection. Results obtained from autofluorescence spectroscopy of benign and malignant lower part gastrointestinal tract (GIT) lesions from freshly excised tissues during surgical removal of the lesions in 18 patients (22 lesions), were compared with the spectral measurements obtained during confocal fluorescent microscopy observations of unstained tissue slides using 405 nm excitation. Excitation–emission matrices (EEMs) were used for ex vivo measurements with applied excitation in 280-440 nm spectral region and emission observed between 300 and 700 nm. Synchronous fluorescence spectroscopy (SFS) approach was also applied to improve the spectral resolution of the observed complex emission spectra. Specific fluorescent features observed, related to presence of structural proteins, co-enzymes and endogenous porphyrins in the tissues investigated, allow discriminating normal mucosa from benign polyps and malignant carcinoma lesions with diagnostic accuracy up to 94.4%.

Published
2019

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