1. Purification and Immune Phenotyping of B-1 Cells from Body Cavities of Mice.
- Author
-
Yenson V and Baumgarth N
- Subjects
- Animals, Antigens, CD immunology, B-Lymphocyte Subsets metabolism, B-Lymphocytes immunology, Bone Marrow immunology, Bone Marrow Cells cytology, CD5 Antigens immunology, Mice, Mice, Inbred C57BL, Peritoneal Cavity cytology, Pleural Cavity cytology, B-Lymphocyte Subsets cytology, B-Lymphocyte Subsets immunology, Flow Cytometry methods
- Abstract
B-1 cells are fetal-origin B lymphocytes with unique developmental and functional characteristics that can generate natural, polyreactive antibodies with important functions in tissue homeostasis and immune defense. While B-1 cell frequencies in bone marrow and secondary lymphoid tissues are low, relative high frequencies exist within peritoneal and pleural cavities of mice, including both CD5
+ and CD5- B-1 cells. These cells represent B-1 reservoirs that, when activated, migrate to lymphoid tissues to secrete antibodies and/or cytokines. Here, we outline efficient methods for the extraction and magnetic isolation of CD5+ B-1 cells from the peritoneal and pleural cavities as well as the separation and phenotypic characterization of CD5+ and CD5- B-1 cells by flow cytometry.- Published
- 2021
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