1. The simultaneous separation and determination of six flavonoids and troxerutin in rat urine and chicken plasma by reversed-phase high-performance liquid chromatography with ultraviolet-visible detection.
- Author
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Yang GJ, Liu P, Qu XL, Xu MJ, Qu QS, Wang CY, Hu XY, and Wang ZY
- Subjects
- Animals, Chickens, Flavonoids blood, Flavonoids urine, Hydroxyethylrutoside blood, Hydroxyethylrutoside isolation & purification, Hydroxyethylrutoside urine, Rats, Sensitivity and Specificity, Chromatography, High Pressure Liquid methods, Flavonoids isolation & purification, Hydroxyethylrutoside analogs & derivatives, Spectrophotometry, Ultraviolet methods
- Abstract
The method of high-performance liquid chromatography (HPLC) with UV-vis detection was used and validated for the simultaneous determination of six flavonoids (puerarin, rutin, morin, luteolin, quercetin, kaempferol) and troxerutin in rat urine and chicken plasma. Chromatographic separation was performed using a VP-ODS column (150 mm x 4.6 mm, 5.0 microm) maintained at 35.0 degrees C. The mobile phase was a mixture of water, methanol and acetic acid (57:43:1, v/v/v, pH 3.0) at the flow rate of 0.8 mL/min. Six flavonoids and troxerutin were analyzed simultaneously with good separation. On optimum conditions, calibration curves were found to be linear with the ranges of 0.10-70.00 microg/mL (puerarin, rutin, morin, luteolin, quercetin, kaempferol) and 0.50-350.00 microg/mL (troxerutin). The detection limits were 0.010-0.050 microg/mL. The method was validated for accuracy and precision, and it was successfully applied to determine drug concentrations in rat urine and chicken plasma samples from rat and chicken that had been orally administered with six flavonoids and troxerutin.
- Published
- 2007
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