1. Liquiritigenin prevents Staphylococcus aureus -mediated lung cell injury via inhibiting the production of α-hemolysin.
- Author
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Dai, Xiao-Han, Li, Hong-En, Lu, Chong-Jian, Wang, Jiang-Feng, Dong, Jing, Wei, Jing-Yuan, Zhang, Yu, Wang, Xin, Tan, Wei, Deng, Xu-Ming, Zhao, Shu-Hua, and Zhang, Ming-Jun
- Subjects
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BIOLOGICAL assay , *CELL culture , *FLAVONOIDS , *GLYCYRRHIZA , *HEMOLYSIS & hemolysins , *LUNGS , *MOLECULAR structure , *POLYMERASE chain reaction , *RESEARCH funding , *STAPHYLOCOCCUS aureus , *T-test (Statistics) , *TOXICITY testing , *TOXINS , *WESTERN immunoblotting , *REVERSE transcriptase polymerase chain reaction , *DATA analysis software , *DESCRIPTIVE statistics - Abstract
Staphylococcus aureus is a significant Gram-positive bacterium that is associated with a broad spectrum of diseases ranging from minor skin infections to lethal pneumonia, endocarditis, and toxinoses. α-Hemolysin is one of the most important exotoxins that contribute to the pathogenesis of S. aureus infections. Liquiritigenin is one of the most significant active components in licorice. In this study, hemolysis, western blot, and real-time reverse transcription-PCR assays were performed to investigate the impact of liquiritigenin on the production of S. aureus α-hemolysin. The results showed that low concentrations of liquiritigenin remarkably decreased S. aureus α-hemolysin production in a dose-dependent manner. Using live/dead cell staining and lactate dehydrogenase assays, we found that liquiritigenin could protect human lung cells (A549) from α-hemolysin-mediated injury. The data indicated that this compound could potentially be useful in developing drugs aiming at staphylococcal α-hemolysin. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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