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A strain of genus Pseudomonas, LYBRD3-7 was isolated from long-term sulfonylurea herbicides applied wheat-field soil in Linying located in Henan province of China. This strain is a strictly aerobic and Gram-negative short rod-shaped bacterium with single flagellum. Phylogenetic evaluation based on 16S rRNA gene sequence analysis placed this isolate as a member of Pseudomonas, and most closely to Pseudomonas tuomuerensis CGMCC 1.1365 (97.1 %) and P. alcaligenes IAM12411 (97.1 %). Morphological characters and chemotaxonomic data confirmed the affiliation of strain LYBRD3-7 to the genus Pseudomonas. The results of phylogenetic analysis, physiological and biochemical studies, and DNA-DNA hybridization allowed the differentiation of genotype and phenotype between strain LYBRD3-7 and the phylogenetic closest species with valid names. The name proposed for the new species is Pseudomonas linyingensis sp. nov. The type strain is LYBRD3-7 (=CGMCC 1.10701 =LMG 25967). [ABSTRACT FROM AUTHOR]

A Gram-negative, non-mobile, polar single flagellum, rod-shaped bacterium WZBFD3-5A2 was isolated from a wheat soil subjected to herbicides for several years. Cells of strain WZBFD3-5A2 grow optimally on Luria-Bertani agar medium at 30 °C in the presence of 0-4.0 % (w/v) NaCl and pH 8.0. 16S rRNA gene sequence analysis revealed that strain WZBFD3-5A2 belongs to the genus Pseudomonas. Physiological and biochemical tests supported the phylogenetic affiliation. Strain WZBFD3-5A2 is closely related to Pseudomonas nitroreducens IAM1439, sharing 99.7 % sequence similarity. DNA-DNA hybridization experiments between the two strains showed only moderate reassociation similarity (33.92 ± 1.0 %). The DNA G+C content is 62.0 mol%. The predominant respiratory quinine is Q-9. The major cellular fatty acids present are C (28.55 %), C or C (20.94 %), C (17.21 %) and C (13.73 %). The isolate is distinguishable from other related members of the genus Pseudomonas on the basis of phenotypic and biochemical characteristics. From the genotypic, chemotaxonomic and phenotypic data, it is evident that strain WZBFD3-5A2 represents a novel species of the genus Pseudomonas, for which the name Pseudomonas nitritereducens sp. nov. is proposed. The type strain is WZBFD3-5A2 (=CGMCC 1.10702 = LMG 25966). [ABSTRACT FROM AUTHOR]