1. FoxL2 combined with Cyp19a1a regulate the spawning upstream migration in Coilia nasus.
- Author
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Fang DA, Yang XJ, Feng X, Zhou YF, Xu DP, Zhang MY, and Liu K
- Subjects
- Animals, Aromatase genetics, Brain growth & development, Brain metabolism, Cytoplasm metabolism, Female, Fishes genetics, Fishes metabolism, Forkhead Box Protein L2 genetics, Gene Expression Regulation, Developmental, Ovary metabolism, Tissue Distribution, Up-Regulation, Aromatase metabolism, Fishes growth & development, Forkhead Box Protein L2 metabolism, Ovary growth & development
- Abstract
FoxL2 is a member of the forkhead/HNF-3-related family of transcription factors which provides tissue specific gene regulation. It is known to regulate ovarian aromatase, which plays a crucial role in ovarian development and mature. To understand the role of FoxL2/ovarian aromatase encoded gene Cyp19a1a during ovarian development and recrudescence, we identified cDNA characteristics of FoxL2 and Cyp19a1a, analyzed its temporal expression both at transcript and protein levels in the anadromous fish, Coilia nasus. Tissue distribution pattern revealed that FoxL2 mRNA expression level was highest in ovary, while Cyp19a1a mRNA was highest in brain. During the upstream migration cycle, in ovary, the FoxL2 mRNA temporal expression peaked at the multiplication stage (stage III in May), the Cyp19a1a mRNA expression peaked at the onset stage (stage I in March). It was found that their mRNA transcripts were maintained at high level during the migration stage (from stage I in March to stage VI in July). Additionally, the strongest immunolabeling positive signals of Cyp19a1a and FoxL2 proteins were mainly found in the cytoplasm of olfactory bulb cell, stratum granulare and neurogliocyte cells and development stage oocytes. Data indicated that FoxL2 and Cyp19a1a were inducible and functional in the C. nasus ovary development and migration process. Therefore, the present results can be regarded as evidence for indispensable roles of FoxL2 and Cyp19a1a in the ovary development and migratory behavior at gene expression patterns and encoded protein distribution level., (Copyright © 2019 Elsevier B.V. All rights reserved.)
- Published
- 2019
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