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The prevalence of extreme, short-term temperature spikes in coastal regions during summer months is predicted to increase with ongoing climate change. In tropical systems, these changes are predicted to increase the metabolic demand of coral reef fish larvae while also altering the plankton communities upon which the larvae feed during their pelagic phase. The consequences of these predictions remain speculative in the absence of empirical data on the interactive effects of warm temperatures on the metabolism, postprandial processes and growth responses of coral reef fish larvae. Here, we tested the effect of increased temperature on the metabolism, postprandial performance and fine-scale growth patterns of a coral reef fish (Amphiprion percula) in the latter half of its ~11-d larval phase. First, we measured the length and weight of fed versus fasted larvae (N = 340; mean body mass 4.1±0.05 mg) across fine temporal scales at a typical current summer temperature (28.5°C) and a temperature that is likely be encountered during warm summer periods later this century (31.5°C). Second, we measured routine metabolic rate (Mo2 routine) and the energetics of the postprandial processes (i.e., digestion, absorption and assimilation of a meal; termed specific dynamic action (SDA)) at both temperatures. Larvae fed voraciously when provided with food for a 12-hour period and displayed a temperature-independent increase in mass of 40.1% (28.5°C) and 42.6% (31.5°C), which was largely associated with the mass of prey in the gut. A subsequent 12-h fasting period revealed that the larvae had grown 21.2±4.8% (28.5°C) and 22.8±8.8% (31.5°C) in mass and 10.3±2.0% (28.5°C) and 7.8±2.6% (31.5°C) in length compared with pre-feeding values (no significant temperature effect). Mo2 routine was 55±16% higher at 31.5°C and peak Mo2 during the postprandial period was 28±11% higher at 31.5°C, yet elevated temperature had no significant effect on SDA (0.51±0.06 J at 28.5°C vs. 0.53±0.07 J at 31.5°C), SDA duration (6.0±0.6 h vs. 6.5±0.5 h), or the percent of total meal energy used for SDA (SDA coefficient: 10.1±1.3% vs. 13.0±1.7%). Our findings of higher Mo2 routine but similar SDA coefficient at high temperature provide the first empirical evidence that coral reef fish larvae may have to secure more food to attain similar growth rates during warm summer periods, and perhaps with chronically warmer conditions associated with climate change. [ABSTRACT FROM AUTHOR]

Concentrations of the paralytic shellfish toxins C1+2, C3+4, GTX5, GTX6, dcGTX2+3, dcSTX, dcNEO, GTX2+3, GTX1+4, STX and NEO were determined by LC-FLD in composite samples of digestive glands and gills of Cerastoderma edule cockle. The specimens were sampled in Aveiro lagoon, Portugal, under natural depuration conditions (days 0, 8, 12, 14, 19, 21 and 25) after exposure to a bloom of Gymnodinium catenatum . Individual paralytic shellfish toxins indicated different pathways of elimination and biotransformation in digestive gland and gills. Toxin concentrations in gills were lower than in digestive gland. Most of the quantified toxins in digestive gland decreased during the 25 days of observation according to negative exponential curves, and only GTX5, GTX6 and NEO showed slight irregularities with time. Concentrations of C1+2, C3+4 and dcGTX2+3 in gills decreased progressively, however GTX5, GTX6 and dcSTX showed pronounced increases. Higher concentrations of those toxins in days 8 and 12 in comparison to the initial value (day 0) indicate conversion of other toxins into GTX5, GTX6 and dcSTX during those periods. It appears that inter-conversion of toxins occurs as G . catenatum cells are retained in gills before being transferred to other compartments. [ABSTRACT FROM AUTHOR]

The structure and histochemical properties of the intestine in bronze corydoras ( Corydoras aeneus), a stomach-containing teleost, are described, with emphasis on goblet cells and mucin types. The proximal intestine displayed a normal structure for teleosts, whereas the distal intestine was wide, translucent, thin-walled, richly vascularized and constantly filled with air, suggesting an important respiratory role. Goblet cells were common throughout the entire intestine and displayed a variable, but mainly faint metachromatic colour after toluidine blue. They were moderately coloured by alcian blue at both pH 2.5 and 0.2 and displayed no colour after periodic acid followed by Schiff's solution (PAS), but a distinct purple-brown colour after high iron diamine followed by alcian blue (p H 2.5). Together, these results suggest that the mucin in the intestine goblet cells consists mainly of sulphated proteoglycans. Further, the results from the present lectin and neuraminidase tests suggest that these mucins contain much N-acetylglucoseamines and some N-acetylgalactosamines and sialic acid, but seem to lack glucose and mannose. They also contain some galactose- N-acetylgalactosamines sequences, normally hidden by sialic acid. The distinct brush border and mucus layer on the epithelial cells in the respiratory intestine may indicate some digestive roles, such as absorption of water, ions and simple carbohydrates. As sulphated proteoglycans are tough and attract much water, this mucus may play important roles in the protection against mechanical and chemical damages and in the defence against micro-organisms throughout the entire intestine, but in the respiratory intestine it may impede significantly the oxygen uptake. However, as this part of the intestine usually contains no digesta, but is completely filled with air, frequently renewed by dry air from the atmosphere, and the main function of the mucus may be to protect the respiratory epithelium against a destroying and dangerous desiccation. [ABSTRACT FROM AUTHOR]

This study was conducted to determine the ontogenetic development of the digestive tract and its accessory structures (liver, pancreas, and gall bladder) in agastric larval Chinese sucker Myxocyprinus asiaticus with the histological and ultrastructural approaches from hatching to 56 days after hatching ( DAH). On the basis of its feeding mode, and analyzing the main histological features of the digestive system, larval development in Chinese sucker was divided into three stages from hatching: stage 1 (endotrophic period): 1-6 DAH; stage 2 (endoexotrophic period): 7-14 DAH; stage 3 (exclusively exotrophic period): from 15 DAH onward. At hatching, the digestive tract of the larvae consisted of an undifferentiated straight tube. At 4 DAH, the mouth opened, and the digestive tract was differentiated into buccopharyngeal cavity, esophagus and intestine. At 7 DAH, fish started to feed exogenously. Yolk sac was completely exhausted at 15 DAH. Until 56 DAH, the digestive tract of the larvae displayed regularly arranged microvilli, abundant vacuoles, and protein inclusion bodies. The pancreas, liver, and gall bladder were functional from 6 DAH, which enabled larvae to ingest, digest, and assimilate the first exogenous food. In comparison with teleosts that have a stomach, the development of the digestive tract of the agastric Chinese sucker seemed relatively slow. [ABSTRACT FROM AUTHOR]

Abstract: Ration levels were used to determine the digestible protein (DP) and amino acids as well as digestible (DE) and metabolizable energy (ME) requirements of Atlantic salmon post-smolts for maintenance and the efficiency of utilization for gain above maintenance. The salmon (96g) were held in seawater at a temperature of 10°C and were fasted for 28days or fed incremental ration levels of a fishmeal-based diet for 51days. The composition of gain was determined by the comparative slaughter technique. The relationships between intake of digestible or metabolizable nutrients or energy and gain of those nutrients or energy were linear (R 2 ≥0.98) between zero intake and maximum voluntary intake. The DE and ME requirements for maintenance were 31.5 and 30.6kJkg−0.8 d−1, respectively, and the efficiency of utilization of DE and ME for gain were 0.80±0.01 and 0.82±0.01, respectively. The efficiency of utilization for gain of DP was 0.64±0.01 and that of the sum of digestible amino acids 0.57±0.02. The efficiency of utilization of the individual amino acids for gain varied from 0.45 for tryptophan to 0.65 for histidine. The maintenance requirement for DP was 117.5mgkg−0.7 d−1 and for digestible sum of amino acids 204.0mgkg−0.7 d−1. The maintenance requirements for the individual amino acids varied from 4.3mgkg−0.7 d−1 for methionine and tryptophan to 20.9mgkg−0.7 d−1 for leucine. The results suggest more emphasis should be directed towards determining the maintenance requirements and the efficiency of utilization for gain above maintenance for the individual amino acids and the sum of amino acids rather than for crude protein. [Copyright &y& Elsevier]

Abstract: We examined the growth performance, tissue selenium (Se) concentration, and histopathology of Sacramento splittail (Pogonichthys macrolepidotus) fed a control diet (0.4 μg Se/g) for 1, 3, 7, 13, 21 wk after a 9-month dietary exposure to 0.4, 12.6, 26.0, and 57.6 μg Se/g dry diet. Splittail previously fed 57.6 μg Se/g showed a significantly (P <0.05) lower final body weight but had higher weight gain than fish fed 0.4 μg Se/g diet at the end of the 21-wk depuration study. There were no significant differences in body weight in fish previously fed diets with or less than 26.0 μg Se/g. Liver and muscle Se concentrations decreased significantly in fish previously fed 26.0 and 57.6 μg Se/g diet but did not change significantly in fish fed 12.6 or less μg Se/g diet at the end of 21 wk. Liver Se concentrations dropped to the same concentration as fish fed 0.4 μg Se/g diet after a 13-wk depuration in all treatments. Muscle Se concentrations remained significantly higher in fish previously fed 12.6 or higher μg Se/g diets when compared to fish fed control diet at the end of a 21-wk depuration. Except for the presence of preneoplastic basophilic foci in two fish previously fed 57.6 μg Se/g diet, normal liver morphology was observed in splittail in all treatments at the end of 21-wk depuration. Prevalences of kidney lesions were increased in fish previously fed 26.0 and 57.6 μg Se/g diets at 3 and 7 wk, and decreased at 13 and 21 wk of depuration. No kidney lesions were observed in fish previously fed 12.6 μg Se/g diet or less. In conclusion, growth of splittail previously fed a diet containing 57.6 μg Se/g was still affected at the end of 21-wk depuration. The 21-wk depuration was not long enough for muscle Se concentrations to return to basal levels in fish previously fed 12.6 or more μg Se/g diet. Deleterious health effects of Se persisted in fish previously fed diets with 26.0 or more μg Se/g diet. Current results suggest that splittail that survived the 9-month exposure to 12.6 or less μg Se/g diet under current laboratory conditions is likely to thrive if Se in diet was reduced to control concentration. [Copyright &y& Elsevier]