1. Structures of two major histocompatibility complex class I genes of the rainbow trout (Oncorhynchus mykiss).
- Author
-
Shum BP, Mason PM, Magor KE, Flodin LR, Stet RJ, and Parham P
- Subjects
- Amino Acid Sequence, Animals, Genetic Linkage, Molecular Sequence Data, Phylogeny, Pseudogenes, Sequence Alignment, Sequence Analysis, DNA, Fish Proteins genetics, Genes, MHC Class I, Histocompatibility Antigens Class I genetics, Oncorhynchus mykiss genetics
- Abstract
Here we describe two rainbow trout major histocompatibility complex (MHC) class I genes characterized from lambda phage genomic clones prepared from a single fish. Clone GC71 contains all exons except a leader peptide-encoding exon. An open reading frame is maintained, and thus the gene MhcOnmy-U71 could be expressed in this individual. The class I gene found on clone GC41 lacks exons encoding the leader peptide and cytoplasmic domain. This gene, MhcOnmy-U41p, is a pseudogene due to a deletion in the alpha(2) domain-encoding exon causing premature termination. Both the Onmy-U71 and Onmy-U41p genes are distinguished by long introns between the exons encoding the alpha(1) and alpha(2) domains. Clone GC41 also contains the 3' exons of the LMP7/ PSMB8 gene encoding the gamma-interferon-induced proteosome subunit of rainbow trout.
- Published
- 2002
- Full Text
- View/download PDF