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Objective: This study aimed at evaluating the role of a novel commercial product, MrFeed Pro50 FF (MP50), as a functional ingredient with which to formulate low‐fish‐meal diets for Largemouth Bass Micropterus nigricans. Methods: Seven test diets were examined. A reference diet (RD) contained 400‐g/kg fish meal, and a control diet (C) was formulated by replacing 60% of the fish meal in RD with poultry by‐product meal. The other five diets were formulated by adding MP50 to C at 5.0 (D50), 7.5 (D75), 10.0 (D100), 12.5 (D125), or 15.0 (D150) g/kg. Fish (initial body weight = 9.2 ± 0.1 g) were fed the test diets for 8 weeks. Result: The MP50 supplementation level significantly affected weight gain, final body weight, carbon retention efficiency, energy retention efficiency (ERE), hepatosomatic index (HSI), viscerosomatic index (VSI), the ratio of fish meal consumption to fish production (RCP), and carbon waste. The weight gain of fish fed D50, D75, and D100 was greater than that of fish fed C and D150. The ERE was greater in fish fed D50 and D100 than in fish fed C and D75. The HSI and VSI were greater and nitrogen and carbon wastes were lower for fish fed D50 and D100 than in fish fed C. Fish fed D50, D75, and D100 exhibited greater weight gain and lower RCP values compared with fish fed RD. The weight gain of fish fed RD did not significantly differ from the weight gain of fish fed C, D125, and D150, confirming that the minimum fish meal level could be reduced to 160 g/kg. Conclusion: This study indicates that MP50 at 5.0–10.0 g/kg can be used as a functional ingredient in low‐fish‐meal diets for Largemouth Bass. Impact statementLargemouth Bass is commercially important for inland aquaculture and sportfishing worldwide; however, its commercial farming relies on high fish meal diets. Replacement of dietary fish meal with alternative ingredients can improve sustainability of Largemouth Bass farming by reducing feed cost and depletion of wild fishery resource. This study assessed the effect of MrFeed Pro50 FF supplementation on growth, feed utilization, body composition, and waste outputs of Largemouth Bass fed a low fish meal diet. Our results indicate that MrFeed Pro50 FF supplementation at 5.0–10.0 g/kg could improve growth of Largemouth Bass, suggesting the potential of MrFeed Pro50 FF as a functional ingredient for formulating low fish meal diets. [ABSTRACT FROM AUTHOR]

The complete quantitative essential amino acid (EAA) requirements of aquacultured animals are largely unknown except for a handful of species. This is problematic because formulation of least-cost diets for target animals demands precise knowledge of EAA requirements. One way of approximating EAA requirements is to use correlations between whole-body and/or muscle EAA profiles using essential to total EAA ratios (A/E ratios). This method requires a reference, quantified EAA requirement, usually lysine (Lys). To systematically evaluate the A/E ratio method, muscle AA profiles were measured in 10 species of teleost and whiteleg shrimp. In fish, Lys represented the dominant muscle EAA measured, ranging between 7.06 and 9.58 g/100 g protein, whereas, in shrimp, arginine (Arg) was quantified as the principal EAA. For non-EAA, glutamate (Glu) and glutamine were consistently the NEAA recorded at highest levels whereas, in shrimp, Glu + Gln was highest followed by aspartate (Asp) plus asparagine (Asn). Except for Arg, which was twice that of fish (P < 0.05), whiteleg shrimp expressed similar muscle A/E profiles. Strength of relationship between muscle EAA levels and known requirements were all strongly and positively correlated for non-salmonid species. When using Lys as the reference EAA there were few semblances between measured and estimated EAA requirements. [ABSTRACT FROM AUTHOR]

The purpose of this study was to determine the influence of krill meal (KM) inclusion at various levels (0%, 2.5%, 5%) in plant-based and animal-based feeds, that were fishmeal (FM) and fish oil (FO) free, on Atlantic salmon growth. A FM/FO feed containing 0% KM was the control. Using a 2 × 3 factorial approach, diets were randomly assigned to one of 28 0.5 m3 flow-through tanks (n = 4 tanks per diet) initially stocked with 60 fish (148.4 ± 12.9 g; 23.6 ± 0.8 cm; condition factor (K) = 1.16 ± 0.08) each. Salmon were fed for 90 days using automatic feeders ad libitum. On day 45, stocking densities were reduced to 45 fish per tank by the random removal of 15 individuals to remove any potential of density affecting growth through the trial end. Water temperature, oxygen saturation, pH, and salinity throughout the trial were 11.8 °C, 103.5%, 7.38, and 32.0 g L−1, respectively. Fish fed plant-based feed without KM were lighter (p < 0.05) than all other groups at day 45 and 90, but those fed a plant-based feed with KM had comparable growth and feed intake compared to that of fish fed the control diet. Irrespective of the presence of KM, animal-based feeds achieved comparable weight growth (p > 0.05) to the control and 5% KM plant-based groups, with KM increasing feed intake (p < 0.05). Between day 45 and 90, feed conversion ratios increased in all groups except the control and 0% KM plant-based group, while specific growth rates (SGRs) decreased for all except the 0% KM plant-based diet. Between-group differences (p < 0.05) were also noted for the thermal growth coefficient. No differences were recorded in visceral or intestinal weight, and whole-body lipid levels were identical, proportional for all groups. Although differences (p < 0.05) were apparent in the concentrations of individual fillet fatty acids between groups, a 75 g serving size of any treatment would be sufficient to exceed daily intake recommendations for EPA + DHA. This trial determined that benefit, in terms of feed intake and growth performance, was gained when KM was added to plant-based feeds. However, no such advantage was observed when KM was used with animal-based feeds. [ABSTRACT FROM AUTHOR]

Fishery processing by-products are a large resource from which to produce fishmeal and other products for a variety of uses. In this study, testes meal ( TM) produced from pink salmon processing by-product was evaluated as a functional ingredient in aquafeeds. Nile tilapia and rainbow trout fry were fed five isonitrogenous and isoenergetic experimental diets for 4 and 9 weeks respectively. Two diets were fishmeal-based ( FM) and three were plant protein-based ( PP). Salmon TM was added to the FM and PP diets at 7% to replace 20% of fishmeal protein ( FMTM and PPTM respectively). An additional control diet was prepared in which fishmeal was added to the PP diet to supply an equivalent amount of protein as supplied by TM ( PPFM). Inclusion of TM in both the FM- and PP-based diets resulted in higher final body weights, although differences were only significant between rainbow trout fed FM or FMTM diets. Similar differences were calculated for other indices of fish performance, e.g. specific growth rate, feed conversion ratio, protein efficiency ratio and protein retention efficiency. Feed intake was significantly higher for fish fed FMTM compared with FM in rainbow trout. For tilapia, final weights were numerically higher, but not significantly different for fish fed diets containing TM compared with non- TM diets ( FM vs. FMTM; PP vs. PPTM). Performance of trout or tilapia fed the PPFM diet did not increase compared with the PP diet. The results indicate that TM addition to both FM and PP diets increased feed intake and also increased metabolic efficiency, demonstrating that TM can be a functional ingredient in aquafeeds. [ABSTRACT FROM AUTHOR]

Eight strains of rainbow trout were introgressed to develop a single strain (H- ARS) that was selected for faster growth when fed a fishmeal-free, plant-based diet (Selection Diet). For four generations, families from these crosses were fed the Selection Diet and selected for increased weight gain. Growth and nutrient retention were compared among H- ARS and two parental strains, the House Creek ( HSC) and Fish Lake ( FL) fed either a fish meal or Selection diet for 12 weeks. There was a significant effect of strain ( P < 0.01), but not diet on weight gain, and a significant interaction of strain by diet ( P < 0.05). The H- ARS trout gained more weight averaged across diet (991% of initial wt.) than the HC (924%) or FL trout (483%). The FL trout fed the fish meal diet gained more weight than FL trout fed the selection diet (510% vs 456%). Conversely, H- ARS trout fed the plant-based diet gained more weight than those fed the fish meal diet (1009% vs 974%). HSC trout had similar weight gain fed either diet (922% vs 926%). A significant effect of strain on protein retention ( P < 0.01) was observed, along with a significant strain by diet interaction ( P < 0.02). The results demonstrate that rainbow trout can be selectively improved to grow on a plant-based diet. [ABSTRACT FROM AUTHOR]

Abstract: We report performance of rainbow trout (Oncorhynchus mykiss) fed a balanced dietary mix of plant proteins supplemented with either fish bone meal (FBM) derived from Alaskan seafood processing byproducts or dicalcium phosphate. Seven experimental diets were formulated to contain two levels of dicalcium phosphate or two levels of two different kinds of FBM in all plant-protein (APP) based diets as follows: Diet 1, FM based; Diets 2 and 3, APP diets with low or high dicalcium phosphate; Diets 4 and 5, APP diets with 4% or 8% low phosphorus FBM; and Diets 6 and 7, APP diets with 2.7% or 5.4% high phosphorus FBM. The limiting amino acids methionine and lysine, as well as the amino sulfonic acid taurine, were added to the APP diets at appropriate concentrations. Triplicate groups of juvenile rainbow trout (average weight 31.5g) were fed one of the seven experimental diets for 12weeks. Negative effects on growth performance and feed utilization were observed in the fish fed both kinds of FBM or the low level of dicalcium phosphate supplemented to APP diets. Fish fed high levels of dicalcium phosphate showed growth performance and feed utilization that was not significantly different from the control group indicating that FM may be completely replaced by plant proteins with appropriate dietary supplements. Significantly decreased ash levels and increased lipid content were observed in fish fed diets supplemented with FBM or dicalcium phosphate at low levels. Whole-body phosphorus level was not significantly different between controls and fish fed APP diets supplemented with dicalcium phosphate whereas phosphorus levels were significantly lower in FBM fed fish. The apparent digestibility coefficients (ADC) of protein and phosphorus were lower in the control diet than diet 3. The digestibility of the two kinds of FBM was not different. Therefore, the results indicate that total replacement of dietary FM solely by plant proteins could be possible in rainbow trout with no apparent reduction in growth performance or feed utilization when a balanced mix of plant protein ingredients is properly supplemented with highly available inorganic phosphate, limiting amino acids and taurine. Maintenance of the body Ca/P ratio close to 1 was also found in rainbow trout although the differences in diets were considerable. Alaskan FBM could be used in fish feeds as a supplemental calcium source but not as the primary source of phosphorus because of the low bioavailability of phosphorus to fish in these diets. [Copyright &y& Elsevier]