1. Cloning and preliminary verification of telomere-associated sequences in upland cotton
- Author
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Yangyang Wei, Yanjun Wang, Zhen Liu, Jiaran Shuang, Renhai Peng, and Yuling Liu
- Subjects
0106 biological sciences ,0301 basic medicine ,Asia ,lcsh:QH426-470 ,Sequence analysis ,cloning ,Plant Science ,Biology ,telomere-associated sequence ,01 natural sciences ,Genome ,DNA sequencing ,03 medical and health sciences ,FISH ,Genetics ,medicine ,Plantae ,Cloning ,medicine.diagnostic_test ,Cenozoic ,Chromosome ,G. hirsutum ,Genomics ,G. hirsutum telomere-associated sequence cloning FISH ,Telomere ,lcsh:Genetics ,030104 developmental biology ,Animal Science and Zoology ,Primer (molecular biology) ,Research Article ,010606 plant biology & botany ,Biotechnology ,Fluorescence in situ hybridization - Abstract
Telomeres are structures enriched in repetitive sequences at the end of chromosomes. In this study, using the telomere primer AA(CCCTAAA)3CCC for the single primer PCR, two DNA sequences were obtained from Gossypium hirsutum (Linnaeus, 1753) accession (acc.) TM-1. Sequence analysis showed that the two obtained sequences were all rich in A/T base, which was consistent with the characteristic of the telomere-associated sequence (TAS). They were designated as GhTAS1 and GhTAS2 respectively. GhTAS1 is 489 bp long, with 57.6% of A/T, and GhTAS2 is 539 bp long, with 63.9% of A/T. Fluorescence in situ hybridization results showed that both of the cloned TASs were located at the ends of the partial chromosomes of G. hirsutum, with the strong signals, which further confirmed that GhTAS1 and GhTAS2 were telomere-associated sequences including highly tandemly repetitive sequences. Results of blast against the assembled genome of G. hirsutum showed that GhTAS sequences may be missed on some assembled chromosomes. The results provide important evidence for the evaluation of the integrity of assembled chromosome end sequences, and will also contribute to the further perfection of the draft genomes of cotton.
- Published
- 2020