9 results on '"Yoshimi, Akira"'
Search Results
2. Cell Wall Integrity and Its Industrial Applications in Filamentous Fungi.
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Yoshimi, Akira, Miyazawa, Ken, Kawauchi, Moriyuki, and Abe, Keietsu
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FILAMENTOUS fungi , *FUNGAL cell walls , *ANTIFUNGAL agents , *INDUSTRIAL applications , *PROTEIN kinase C , *YEAST fungi - Abstract
Signal transduction pathways regulating cell wall integrity (CWI) in filamentous fungi have been studied taking into account findings in budding yeast, and much knowledge has been accumulated in recent years. Given that the cell wall is essential for viability in fungi, its architecture has been analyzed in relation to virulence, especially in filamentous fungal pathogens of plants and humans. Although research on CWI signaling in individual fungal species has progressed, an integrated understanding of CWI signaling in diverse fungi has not yet been achieved. For example, the variety of sensor proteins and their functional differences among different fungal species have been described, but the understanding of their general and species-specific biological functions is limited. Our long-term research interest is CWI signaling in filamentous fungi. Here, we outline CWI signaling in these fungi, from sensor proteins required for the recognition of environmental changes to the regulation of cell wall polysaccharide synthesis genes. We discuss the similarities and differences between the functions of CWI signaling factors in filamentous fungi and in budding yeast. We also describe the latest findings on industrial applications, including those derived from studies on CWI signaling: the development of antifungal agents and the development of highly productive strains of filamentous fungi with modified cell surface characteristics by controlling cell wall biogenesis. [ABSTRACT FROM AUTHOR]
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- 2022
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3. Analysis of the self-assembly process of Aspergillus oryzae hydrophobin RolA by Langmuir–Blodgett method.
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Terauchi, Yuki, Tanaka, Takumi, Mitsuishi, Masaya, Yabu, Hiroshi, Yoshimi, Akira, Nantani, Kei, and Abe, Keietsu
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KOJI ,ATOMIC force microscopy ,INTERFACE structures ,AIR-water interfaces ,FILAMENTOUS fungi ,ASPERGILLUS - Abstract
Hydrophobins are small, amphipathic proteins secreted by filamentous fungi. Hydrophobin RolA, which is produced by Aspergillus oryzae, attaches to solid surfaces, recruits the polyesterase CutL1, and consequently promotes hydrolysis of polyesters. Because this interaction requires the N-terminal, positively charged residue of RolA to be exposed on the solid surface, the orientation of RolA on the solid surface is important for recruitment. However, the process by which RolA forms the self-assembled structure at the interface remains unclear. Using the Langmuir–Blodgett technique, we analyzed the process by which RolA forms a self-assembled structure at the air–water interface and observed the structures on the hydrophobic or hydrophilic SiO
2 substrates via atomic force microscopy. We found that RolA formed self-assembled films in two steps during phase transitions. We observed different assembled structures of RolA on hydrophilic and hydrophobic SiO2 substrates. Scheme of RolA Langmuir film transition. [ABSTRACT FROM AUTHOR]- Published
- 2020
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4. Both Galactosaminogalactan and α-1,3-Glucan Contribute to Aggregation of Aspergillus oryzae Hyphae in Liquid Culture.
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Miyazawa, Ken, Yoshimi, Akira, Sano, Motoaki, Tabata, Fuka, Sugahara, Asumi, Kasahara, Shin, Koizumi, Ami, Yano, Shigekazu, Nakajima, Tasuku, and Abe, Keietsu
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KOJI ,BETA-glucans ,FILAMENTOUS fungi ,ASPERGILLUS nidulans ,GLUCANS ,AMINO group ,HYDROGEN bonding - Abstract
Filamentous fungi generally form aggregated hyphal pellets in liquid culture. We previously reported that α-1,3-glucan-deficient mutants of Aspergillus nidulans did not form hyphal pellets and their hyphae were fully dispersed, and we suggested that α-1,3-glucan functions in hyphal aggregation. However, Aspergillus oryzae α-1,3-glucan-deficient (AGΔ) mutants still form small pellets; therefore, we hypothesized that another factor responsible for forming hyphal pellets remains in these mutants. Here, we identified an extracellular matrix polysaccharide galactosaminogalactan (GAG) as such a factor. To produce a double mutant of A. oryzae (AG-GAGΔ), we disrupted the genes required for GAG biosynthesis in an AGΔ mutant. Hyphae of the double mutant were fully dispersed in liquid culture, suggesting that GAG is involved in hyphal aggregation in A. oryzae. Addition of partially purified GAG fraction to the hyphae of the AG-GAGΔ strain resulted in formation of mycelial pellets. Acetylation of the amino group in galactosamine of GAG weakened GAG aggregation, suggesting that hydrogen bond formation by this group is important for aggregation. Genome sequences suggest that α-1,3-glucan, GAG, or both are present in many filamentous fungi and thus may function in hyphal aggregation in these fungi. We also demonstrated that production of a recombinant polyesterase, CutL1, was higher in the AG-GAGΔ strain than in the wild-type and AGΔ strains. Thus, controlling hyphal aggregation factors of filamentous fungi may increase productivity in the fermentation industry. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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5. Functional Analysis of the a-1,3-Glucan Synthase Genes agsA and agsB in Aspergillus nidulans: AgsB Is the Major a-1,3-Glucan Synthase in This Fungus.
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Yoshimi, Akira, Sano, Motoaki, Inaba, Azusa, Kokubun, Yuko, Fujioka, Tomonori, Mizutani, Osamu, Hagiwara, Daisuke, Fujikawa, Takashi, Nishimura, Marie, Yano, Shigekazu, Kasahara, Shin, Shimizu, Kiminori, Yamaguchi, Masashi, Kawakami, Kazuyoshi, and Abe, Keietsu
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ASPERGILLUS nidulans , *FILAMENTOUS fungi , *BACTERIAL cell walls , *POLYSACCHARIDES , *GLUCANS , *CELLS - Abstract
Although α-1,3-glucan is one of the major cell wall polysaccharides in filamentous fungi, the physiological roles of α-1,3-glucan remain unclear. The model fungus Aspergillus nidulans possesses two α-1,3-glucan synthase (AGS) genes, agsA and agsB. For functional analysis of these genes, we constructed several mutant strains in A. nidulans: agsA disruption, agsB disruption, and double-disruption strains. We also constructed several CagsB strains in which agsB expression was controlled by the inducible alcA promoter, with or without the agsA-disrupting mutation. The agsA disruption strains did not show markedly different phenotypes from those of the wild-type strain. The agsB disruption strains formed dispersed hyphal cells under liquid culture conditions, regardless of the agsA genetic background. Dispersed hyphal cells were also observed in liquid culture of the CagsB strains when agsB expression was repressed, whereas these strains grew normally in plate culture even under the agsB-repressed conditions. Fractionation of the cell wall based on the alkali solubility of its components, quantification of sugars, and 13 C-NMR spectroscopic analysis revealed that α-1,3-glucan was the main component of the alkali-soluble fraction in the wild-type and agsA disruption strains, but almost no α-1,3-glucan was found in the alkali-soluble fraction derived from either the agsB disruption strain or the CagsB strain under the agsB-repressed conditions, regardless of the agsA genetic background. Taken together, our data demonstrate that the two AGS genes are dispensable in A. nidulans, but that AgsB is required for normal growth characteristics under liquid culture conditions and is the major AGS in this species. [ABSTRACT FROM AUTHOR]
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- 2013
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6. Functional analysis of basidiomycete specific chitin synthase genes in the agaricomycete fungus Pleurotus ostreatus.
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Schiphof, Kim, Kawauchi, Moriyuki, Tsuji, Kenya, Yoshimi, Akira, Tanaka, Chihiro, Nakazawa, Takehito, and Honda, Yoichi
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PLEUROTUS ostreatus , *FUNGAL cell walls , *CHITIN synthase , *FUNCTIONAL analysis , *HOMOLOGOUS recombination , *MEMBRANE proteins , *FILAMENTOUS fungi - Abstract
• Three clades of basidiomycete specific chitin synthases were identified. • Disruption of chsb2–4 impacts hyphal morphology and cell wall thickness. • Δc hsb2–4 strains are sensitive to cell wall and membrane, not oxidative stressors. Chitin is an essential structural component of fungal cell walls composed of transmembrane proteins called chitin synthases (CHSs), which have a large range of reported effects in ascomycetes; however, are poorly understood in agaricomycetes. In this study, evolutionary and molecular genetic analyses of chs genes were conducted using genomic information from nine ascomycete and six basidiomycete species. The results support the existence of seven previously classified chs clades and the discovery of three novel basidiomycete-specific clades (BI–BIII). The agaricomycete fungus Pleurotus ostreatus was observed to have nine putative chs genes, four of which were basidiomycete-specific. Three of these basidiomycete specific genes were disrupted in the P. ostreatus 20b strain (ku80 disruptant) through homologous recombination and transformants were obtained (Δ chsb2 , Δ chsb3 , and Δ chsb4). Despite numerous transformations Δ chsb1 was unobtainable, suggesting disruption of this gene causes a crucial negative effect in P. ostreatus. Disruption of these chsb2–4 genes caused sparser mycelia with rougher surfaces and shorter aerial hyphae. They also caused increased sensitivity to cell wall and membrane stress, thinner cell walls, and overexpression of other chitin and glucan synthases. These genes have distinct roles in the structural formation of aerial hyphae and cell walls, which are important for understanding basidiomycete evolution in filamentous fungi. [ABSTRACT FROM AUTHOR]
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- 2024
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7. Improved recombinant protein production in Aspergillus oryzae lacking both α-1,3-glucan and galactosaminogalactan in batch culture with a lab-scale bioreactor.
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Ichikawa, Hikaru, Miyazawa, Ken, Komeiji, Keisuke, Susukida, Shunya, Zhang, Silai, Muto, Kiyoaki, Orita, Ryutaro, Takeuchi, Ayumu, Kamachi, Yuka, Hitosugi, Masahiro, Yoshimi, Akira, Shintani, Takahiro, Kato, Yoshikazu, and Abe, Keietsu
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KOJI , *METABOLITES , *PROPERTIES of fluids , *ORGANIC acids , *FILAMENTOUS fungi , *RECOMBINANT proteins , *GLUCANS , *BETA-glucans - Abstract
Filamentous fungi are used as production hosts for various commercially valuable enzymes and chemicals including organic acids and secondary metabolites. We previously revealed that α-1,3-glucan and galactosaminogalactan (GAG) contribute to hyphal aggregation in the industrial fungus Aspergillus oryzae , and that production of recombinant protein in shake-flask culture is higher in a mutant lacking both α-1,3-glucan and GAG (AGΔ-GAGΔ) than in the parental strain. Here, we compared the productivity of the wild type, AGΔ-GAGΔ, and mutants lacking α-1,3-glucan (AGΔ) or GAG (GAGΔ) in batch culture with intermittent addition of glucose in a 5-L lab-scale bioreactor. The hyphae of the wild type and all mutants were dispersed by agitation, although the wild type and AGΔ formed small amounts of aggregates. Although mycelial weight was similar among the strains, the concentration of a secreted recombinant protein (CutL1) was the highest in AGΔ-GAGΔ. Evaluation of fluid properties revealed that the apparent viscosities of mycelial cultures of the wild type and AGΔ-GAGΔ decreased as the agitation speed was increased. The apparent viscosity of the AGΔ-GAGΔ culture tended to be lower than that of the wild-type strain at each agitation speed, and was significantly lower at 600 rpm. Overall, the lack of α-1,3-glucan and GAG in the hyphae improved culture rheology, resulting in an increase in recombinant protein production in AGΔ-GAGΔ. This is the first report of flow behavior improvement by a cell-surface component defect in a filamentous fungus. [Display omitted] [ABSTRACT FROM AUTHOR]
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- 2022
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8. Class of cyclic ribosomal peptide synthetic genes in filamentous fungi.
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Nagano, Nozomi, Umemura, Myco, Izumikawa, Miho, Kawano, Jin, Ishii, Tomoko, Kikuchi, Moto, Tomii, Kentaro, Kumagai, Toshitaka, Yoshimi, Akira, Machida, Masayuki, Abe, Keietsu, Shin-ya, Kazuo, and Asai, Kiyoshi
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FILAMENTOUS fungi , *RIBOSOMAL RNA , *PEPTIDE synthesis , *FUNGAL proteins , *FUNGAL genes - Abstract
Ustiloxins were found recently to be the first example of cyclic peptidyl secondary metabolites that are ribosomally synthesized in filamentous fungi. In this work, two function-unknown genes ( ustYa / ustYb ) in the gene cluster for ustiloxins from Aspergillus flavus were found experimentally to be involved in cyclization of the peptide. Their homologous genes are observed mainly in filamentous fungi and mushrooms. They have two “HXXHC” motifs that might form active sites. Computational genome analyses showed that these genes are frequently located near candidate genes for ribosomal peptide precursors, which have signal peptides at the N-termini and repeated sequences with core peptides for the cyclic portions, in the genomes of filamentous fungi, particularly Aspergilli , as observed in the ustiloxin gene cluster. Based on the combination of the ustYa / ustYb homologous genes and the nearby ribosomal peptide precursor candidate genes, 94 ribosomal peptide precursor candidates that were identified computationally from Aspergilli genome sequences were classified into more than 40 types including a wide variety of core peptide sequences. A set of the predicted ribosomal peptide biosynthetic genes was experimentally verified to synthesize a new cyclic peptide compound, designated as asperipin-2a, which comprises the amino acid sequence in the corresponding precursor gene, distinct from the ustiloxin precursors. [ABSTRACT FROM AUTHOR]
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- 2016
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9. Transcriptional profiling for Aspergillus nidulans HogA MAPK signaling pathway in response to fludioxonil and osmotic stress
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Hagiwara, Daisuke, Asano, Yoshihiro, Marui, Junichiro, Yoshimi, Akira, Mizuno, Takeshi, and Abe, Keietsu
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GENETIC transcription , *ASPERGILLUS nidulans , *PLANT cellular signal transduction , *CELLULAR signal transduction , *OSMOSIS , *EFFECT of pesticides on plants , *EFFECT of fungicides on plants , *FILAMENTOUS fungi , *OXIDATIVE stress - Abstract
Abstract: In filamentous fungi, the His–Asp phosphorelay signaling system and HOG pathway are involved in the action of the fungicides, fludioxonil, and iprodione, as well as osmotic and oxidative stress responses. Aspergillus nidulans response regulators (RRs), SskA and SrrA, and histidine kinase (HK), NikA, are involved in the growth inhibitory effects of these fungicides. To gain further insights into the molecular basis for these signaling systems, we performed DNA microarray analyses of fludioxonil and osmotic stress responses in A. nidulans. A global expression analysis revealed that a large number of genes were modulated by fludioxonil treatment in an SskA-dependent manner, whereas SrrA hardly contributed to this modulation. The fludioxonil up-regulated or down-regulated genes (FUGs or FDGs, respectively) are also dependent on the HogA MAPK cascade. We found that the SskA–HogA pathway regulates expression of atfA gene encoding a transcription factor involved in conidia stress tolerance. From the results of microarray analyses, AtfA-dependent FUGs largely overlapped with HogA-dependent FUGs, suggesting that AtfA functions downstream of the HogA MAPK. A series of microarray analyses showed that the inferred SskA–HogA–AtfA pathway is implicated in the transcriptional response to osmotic stress as well as fludioxonil. The srrA atfA null double mutant turns off the SrrA and SskA–HogA–AtfA pathways and showed sensitivity to osmotic stress but no resistance to fludioxonil. Our data revealed that the growth inhibitory effect of fludioxonil depends on factors other than AtfA in spite of the fact that AtfA functions downstream of the HogA MAPK cascade. The complexity of the stress response in the His–Asp phosphorelay system followed by the HogA MAPK cascade is discussed. [Copyright &y& Elsevier]
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- 2009
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