1. Comparison of 18 F-FDG, 18 F-Fluoroacetate, and 18 F-FEPPA for Imaging Liver Fibrosis in a Bile Duct-Ligated Rat Model.
- Author
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Wu, Chun-Yi, Hsieh, Hsin-Hua, Chu, Pei-An, Hong, Wen-Hsiang, Chang, Ting-Yu, Hsu, Chia-Fang, Lin, Siao-Ting, Su, Po-Hsun, and Peng, Shin-Lei
- Subjects
ANIMAL disease models ,SPRAGUE Dawley rats ,BILE ducts ,FIBROSIS ,IMMUNOSTAINING ,POSITRON emission tomography - Abstract
Developing sensitive diagnostic methods for a longitudinal evaluation of the status of liver fibrosis is a priority. This study is aimed at assessing the significance of longitudinal positron emission tomography (PET) imaging with
18 F-labeling tracers for assessing liver fibrosis in a rat model with bile duct ligation (BDL). Twenty-one 6-week-old Sprague-Dawley male rats were used in this study. Longitudinal PET images using [18 F]N-2-(2-fluoroethoxy)benzyl)-N-(4-phenoxypyridin-3-yl)acetamide ([18 F]FEPPA) (n = 3), [18 F]fluoroacetate ([18 F]FAc) (n = 3), and 18F-fluoro-2-deoxy-D-glucose ([18 F]FDG) (n = 3) were obtained at 0, 1, and 2 weeks after BDL. Biochemical assays, histological assays, immunohistochemical staining assays, and next generation sequencing analyses were also performed at 0 (n = 3), 1 (n = 3), 2 (n = 3), and 3 (n = 3) weeks after BDL, which demonstrated the severe damage in rat livers after BDL. Regarding [18 F]FEPPA and [18 F]FDG, there was a significantly higher uptake in the liver after BDL (both P < 0.05), which lasted until week 2. However, the uptake of [18 F]FAc in the liver was not significantly different before and after BDL (P = 0.28). Collectively, both [18 F]FEPPA and [18 F]FDG can serve as sensitive probes for detecting the liver fibrosis. However, [18 F]FAc is not recommended to diagnose liver fibrosis. [ABSTRACT FROM AUTHOR]- Published
- 2021
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