1. Conditioned medium from 3D culture system of stromal vascular fraction cells accelerates wound healing in diabetic rats.
- Author
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Deng C, He Y, Feng J, Dong Z, Yao Y, and Lu F
- Subjects
- Animals, Culture Media, Conditioned chemistry, Culture Media, Conditioned pharmacology, Female, Humans, Rats, Rats, Sprague-Dawley, Stromal Cells metabolism, Adipose Tissue metabolism, Diabetes Mellitus, Experimental drug therapy, Diabetes Mellitus, Experimental metabolism, Fibroblasts metabolism, Intercellular Signaling Peptides and Proteins chemistry, Intercellular Signaling Peptides and Proteins pharmacology, Keratinocytes metabolism, Wounds and Injuries drug therapy, Wounds and Injuries metabolism
- Abstract
Aim: We investigated the healing effects of conditioned medium (CM) derived from a physiological 3D culture system engineered to use an extracellular matrix/stromal vascular fraction (SVF) gel enriched for adipose on diabetic wounds in rats. This CM (Gel-CM) was compared with that from a 2D culture system that used SVF cells (SVF-CM). Materials & methods: Keratinocytes, fibroblasts and wounds were treated with Gel-CM and SVF-CM, and cytokine levels in the CM types were quantified. Results: Proliferation and migration of keratinocytes and fibroblasts were significantly higher after treatment with Gel-CM than with SVF-CM. Collagen secretion by fibroblasts and wound closure were highly stimulated by Gel-CM. Proteomic analyses revealed a higher concentration of growth factors in Gel-CM than in SVF-CM. Conclusion: Gel-CM is a promising therapeutic option for treating diabetic wounds.
- Published
- 2019
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