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Agriculture produces food, fiber and biofuels for the world's growing population, however, agriculture can be a major contributor of nitrogen (N) losses including emissions of ammonia (NH 3 ), nitrous oxide (N 2 O) and nitrate (NO 3 - emission from fertilizers, manure from housing, storage and field, as well as direct/indirect N 3 O emissions from fertilizers, manures, crop residues and soil organic matter. The NO 2 O emissions from fertilizers, manures, crop residues and soil organic matter. The NO 3 - leaching is estimated based on the residual soil N (RSN) at harvest and drainage derived with the DeNitrification-DeComposition (DNDC) model. From 1981 to 2016, the N input from fertilizer and N fixation increased at a greater rate than N removal in harvested crops in all provinces of Canada, resulting in an increase in the RSN and N losses. In 2016, the Prairie provinces had lower N losses (11.7 kg N ha -1 ) from N 2 O, NH 3 and NO 3 - compared with 43.2 kg N ha -1 in central Canada, and 76.5 kg N ha -1 in Atlantic Canada. However, the Prairie provinces had 84.3% of the total Canadian farmland (74.3% of the total Canadian N input), while central Canada had 12.9% of Canadian farmland (21.7% of the total Canadian N input). In the Prairie provinces, the total N 2 O loss from fertilizer N ranged 4.4-8.6 Gg N whereas NH 3 loss ranged from 17.1 to 44.6 Gg N and these values were influenced by both emission intensity and total land area. Total N 2 O losses from manure were highest in Alberta, Ontario and Quebec resulting in 4.8, 4.4, and 3.4 Gg N and NH 3 losses from manure were also highest in these 3 provinces at 61.1, 45.2 and 40.4 Gg N, respectively. Nitrate leaching was impacted by drainage volumes, soil type and N inputs. In the non-growing season, NO 3 - leaching losses (36-yr average) were 63.3 Gg in Ontario and 57.5 Gg N in Quebec compared with 20.8 Gg N for Ontario and 35.5 Gg N for Quebec in the growing season. In contrast, the Prairie provinces showed higher NO 3 - leaching in the growing season (23.1-37.4 Gg N) than in the non-growing season (10.4-13.7 Gg N). In summary, total fertilizer N increased the most over the 36 years in the Prairies which resulted in increased RSN and N leaching losses that will require further intervention., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

Nutrient circularity can help supply chain participants meet sustainability targets. Across the segmented beef supply chain, opportunity exists to reinforce and introduce nutrient circularity by recycling surplus manure nutrients from cattle feedlots to lands where cattle feed is produced. We describe four datasets developed to evaluate options in U.S. and Canadian beef systems. The datasets delineate three "circular manuresheds," each encompassing a hay-grazing landscape where beef cattle are raised on grazingland and supplemented with hay grown nearby, and the distant feedlots where those cattle produce manure nutrients for potential import back to the hayfields. We selected the hay-grazing landscapes of New Mexico, USA; Florida, USA; and western Canada (Manitoba, Saskatchewan, Alberta, British Columbia) because of their significant grazingland production and potential to substitute feedlot manure for commercial fertilizer on hayfields. In each circular manureshed, the manure nutrients from major feedlot destinations could supply a considerable proportion of the P used by hay for grazing cattle: 34% of the P requirements in New Mexico, 36% in Florida, and 6% in western Canada. The average distance to return the resource was 647 km for New Mexico, 1,884 km for Florida, and 1,587 km for western Canada. These magnitudes and distances suggest that the New Mexico circular manureshed may be the most economically viable in the current agri-food system, but this reflects only part of a greater, multi-factor assessment of tradeoffs. The circular manureshed concept provides a platform for simultaneous consideration of competing factors for sustainability via circularity., (© 2022 Her Majesty the Queen in Right of Canada. Journal of Environmental Quality published by Wiley Periodicals LLC on behalf of American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America. Reproduced with the permission of the Minister of Agriculture and Agri-Food Canada. This article has been contributed to by U.S. Government employees and their work is in the public domain in the USA.)

The specialization and intensification of agriculture have produced incredible gains in productivity, quality, and availability of agricultural commodities but have resulted in the separation of crop and animal production. A by-product of this separation has been the accumulation of manure regions where animal production is concentrated. Enter the "manureshed," an organizing framework for integrating animal and crop production where budgeting of manure nutrients is used to strategically guide their recycling and reuse in agricultural production systems where manure resources are of highest value. To move beyond regional nutrient balance analyses into the transformational realm required to mitigate "wicked" manure problems, manureshed management requires recognition of the challenges to systematically reorganizing resource flows. In better integrating crop and livestock systems, manureshed management must account for the unique nature of managing manure nutrients within individual livestock industries, anticipate trade-offs in substituting manure for commercial fertilizer, promote technologies to refine manure, and engage extensive social networks across scales that range from the farmgate to nation and beyond., (© 2022 Her Majesty the Queen in Right of Canada and The Authors. Journal of Environmental Quality published by Wiley Periodicals LLC on behalf of American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America. Reproduced with the permission of the Minister of Agriculture of Canada.)

Efficient use of manure nutrients by crops is necessary to minimize losses to the environment. This field study examined the possibility of replacing side-banded mineral P with precision-placed high-P sludge (6.2-11.0% dry matter) obtained after settling dairy manure slurry. The sludge was injected at about 30 kg P ha (36.0-51.2 m ha) into the soil at corn row spacing, and the corn was planted 5, 10, and 15 cm beside the injection furrow. Controls included no added P and side-banded commercial P fertilizer. The treatments were tested on corn with low and high root colonization by arbuscular mycorrhizae (AM). The study showed that sludge did not impede AM root colonization, corn germination, or seedling growth. Corn plants with both high and low levels of AM colonization responded to the sludge from the three-leaf stage and showed the greatest benefit at the six-leaf stage. Corn responded more to sludge placed at 5 than at 15 cm from the corn rows, whereas the response at the 10-cm spacing was intermediate. There was little difference in seedling growth or final harvest parameters between the side-banded fertilizer P and the 5-cm sludge treatment. The results show a new way to use manure nutrients, namely precision-placement sludge for corn. This may obviate the need for chemical fertilizers for improving farm nutrient balances. Other anticipated benefits are less energy use for hauling and injection of the sludge fraction and reduced risk of nutrient loss by runoff and volatilization (ammonia) and nuisance odors due to injection., (Copyright © by the American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America, Inc.)

Treatment of liquid swine manure (LSM) offers opportunities to improve manure nutrient management. However, N2O fluxes and cumulative emissions resulting from application of treated LSM are not well documented. Nitrous oxide emissions were monitored following band-incorporation of 100 kg N ha(-1) of either mineral fertilizer, raw LSM, or four pretreated LSMs (anaerobic digestion; anaerobic digestion + flocculation: filtration; decantation) at the four-leaf stage of corn (Zea mays L.). In a clay soil, a larger proportion of applied N was lost as N2O with the mineral fertilizer (average of 6.6%) than with LSMs (3.1-5.0%), whereas in a loam soil, the proportion of applied N lost as N2O was lower with the mineral fertilizer (average of 0.4%) than with LSMs (1.2-2.4%). Emissions were related to soil NO3 intensity in the clay soil, whereas they were related to water-extractable organic C in the loam soil. This suggests that N2O production was N limited in the clay soil and C limited in the loam soil, and would explain the interaction found between N sources and soil type. The large N2O emission coefficients measured in many treatments, and the contradicting responses among N sources depending on soil type, indicate that (i) the Intergovernmental Panel on Climate Change (IPCC) default value (1%) may seriously underestimate N2O emissions from fine-textured soils where fertilizer N and manure are band-incorporated, and (ii) site-specific factors, such as drainage conditions and soil properties (e.g., texture, organic matter content), have a differential influence on emissions depending on N source.

This study investigated the feasibility of altering the dietary cation-anion difference (DCAD) in grass by altering the grass variety and the amount and formulation of K fertilizer application. In experiment 1, treatments were combinations of 2 varieties (Barcel and Hi-Mag) of tall fescue (Festuca arundinacea Schreb); 2 rates of K (0 and 250 kg/ha), supplied as KCl; and 2 rates of Mg (0 and 60 kg/ha), supplied as MgO. In experiment 2, K fertilizer was applied at 0 or 125 kg/ha as KCl or K2SO4. There was no difference between HiMag and Barcel tall fescue in the concentrations of Mg, S, and Cl. Application of K fertilizer decreased concentrations of Mg, but the magnitude of the decrease was not consistent across harvests. Conversely, application of Mg fertilizer increased Mg concentrations, but again, the magnitude of the increase was not consistent across harvests. The concentrations of Ca were higher in HiMag than in Barcel tall fescue, with the magnitude of the difference increasing from first to last harvest. Potassium fertilizer decreased Ca concentrations in the first, fourth, and fifth harvests only. Calcium concentration was decreased by a greater magnitude in HiMag tall fescue as a result of Mg fertilization. The HiMag tall fescue contained lower concentrations of K than did Barcel tall fescue in the first, second, and third harvests. Application of K fertilizer increased the K concentration in all 5 harvests but did not affect Na concentrations except in the last harvest. The HiMag tall fescue contained less Na than did Barcel, but the magnitude of the difference was affected by K and Mg fertilization. Application of K fertilizer decreased S concentrations in first-harvest grass, increased concentrations in second-and third-harvest grasses, and had no effect in fourth-or fifth-harvest grasses. Application of Mg fertilizer decreased S concentrations of tall fescue. Application of K fertilizer increased DCAD values for grass harvested from the second through fifth harvests. The increase in DCAD attributable to K fertilizer was less in HiMag than in Barcel tall fescue. Application of K fertilizer as K2SO4 increased dry matter yield and S concentrations of HiMag tall fescue, whereas K applied as KCl increased concentrations of K and Cl. There was no effect of fertilizer formulation on Na concentrations. The DCAD was lower in HiMag tall fescue fertilized with K2SO4 compared with that fertilized with KCl. This study showed that DCAD of grass can be manipulated by the choice of grass variety, fertilizer formulation, and fertilizer application rate.

The potential exists for natural or synthetic hormonal chemicals present in agricultural fertilizers to be transferred to adjacent aquatic environments in order to alter endocrine function in exposed wildlife. Recombinant yeast and mammalian cell line (BG1Luc4E2) assays were used to screen crude organic extracts of municipal biosolids and animal manures for estrogen-, androgen-, and progesterone receptor gene transcription activities. Of the biosolid extracts, those samples that had undergone aerobic digestion had no or minimal estrogen- and no androgen receptor gene transcription activities. In contrast, those biosolid samples that had undergone anaerobic digestion had much higher estrogen- and, for all but one site, androgen receptor gene transcription activities. Extracts prepared from animal manure samples had variable levels of androgen- and estrogen receptor gene transcription activities, which may be related to the type, sex, age, and reproductive status of the animals. The diet and treatment of animals with hormone implants also appeared to be factors influencing hormone activity in animal manure. Progesterone receptor gene transcription activity was observed for only one chicken litter sample. Overall, results of this study suggest that in vitro bioassays can be used to survey and detect hormone activity in municipal biosolids and animal manures. Furthermore, results of these assays can be used to develop practices that will minimize the potential environmental endocrine-disrupting effects of these substances., (Copyright 2004 Wiley Periodicals, Inc.)

The objective of this study was to monitor ammonia emissions and develop updated ammonia emission factors for land application of poultry manure under British Columbia conditions. Field trials, which involved four types of poultry manure as the fertilizer materials, were performed at Agassiz Research Station in 2005 and 2006. After manure application, ammonia emission rates were determined using wind tunnels, capturing the emitted ammonia with acid traps and analyzing with a flow injection analyzer. For all trials, the highest emissions occurred within the first day, and gradually declined over the next 2-3 weeks. Cumulative ammonia emission in all treatments did not exceed the initial amount of ammonia- nitrogen present in manure. Ammonia emission rates were significantly different among the manure types (p<0.005). The percent total loss of ammonia with time was positively correlated with manure pH. Ammonia emission rates were generally higher in both of the spring trials than the fall trial. The proposed revised ammonia emission factors of 0.12 and 0.16 for the two major types of poultry - broiler and layer - are in line with current emission factors adopted by Environment Canada. However, current and revised emission factors (0.38 vs. 0.13) were substantially different for turkey manure. [ABSTRACT FROM AUTHOR]

Two experiments were conducted to evaluate the potential to use leaf chlorophyll index (LCI), measured using a Minolta SPAD-502 meter, for making fertilizer nitrogen (N) recommendations for silage corn in a high N fertility environment. In Experiment 1, treatments were 0, 40, 80, 120, 160, and 200 kg N ha[sup -1] as NH[sub 4]NO[sub 3] broadcast at corn emergence. At the six-leaf stage, soil inorganic N to 90cm depth, total Kjeldahl N concentration of the fifth leaf, sixth leaf, and the above-ground portion of the plant, and LCI of the fifth and sixth leaves, were determined. In Experiment 2, LCI and LCIR (ratio of LCI to LCI from a reference plot receiving 200 kg N ha[sup -1] at corn emergence) were measured at approximately the six-leaf stage in 87 trials 1994-1996. Treatments consisted of different fertilizer N rates applied at sidedress, and corn yield and pre-sidedress soil nitrate test (PSNT) values were determined. In Experiment 1, nitrogen concentration of the above-ground plant increased with increasing soil inorganic N content up to about 350kg Nha[sup -1], and LCI generally increased linearly with leaf total N concentration. However, the relationship between LCI and leaf N concentration varied among years, and the relationship between leaf total N concentration and above-ground plant total N concentration was inconsistent. In Experiment 2, above-ground plant total N concentration was a poor predictor of soil inorganic N supply and corn relative yield when measured over a number of trials. Leaf chlorophyll index at the six-leaf stage therefore has significant limitations as an indicator of corn N status or soil inorganic N supply. Despite these limitations, LCI had a 68% success rate in identifying fields which were responsive and unresponsive to sidedress N using a critical LCI value of 43.7 SPAD units. This success rate is similar to that obtained with the PSNT. However, LCI cannot be used to recommend rates of fertilizer N for LCI values below the... [ABSTRACT FROM AUTHOR]

• Historical slurry applications to grassland improve corn response to applied N and P. • Historical applications of separated slurry had less corn response than whole slurry. • Historical treatments have impact on corn even with current fertilizer application. • Corn performance responded linearly to historical application rates of organic-N. Removing solids from dairy slurry improves grass N response and reduces soil loading of P and organic N which makes this practice useful for sustainable farming. Therefore, it is important to understand the effects of long-term applications on grass of separated dairy slurry on the growth and N response of a subsequent corn crop. This two-year field study investigated the residual effects after 11 years with applications on a perennial grass (Festuca arundinacea Schreb.) of whole (WS), separated dairy slurry (LF) obtained from the second stage of a two stage lagoon system, mineral fertilizer (Fert), WS plus Fert (WS/Fert), and Control (no fertilizer), on two subsequent crops of silage corn (Zea mays L.). In addition to the legacy nutrients, the corn received commercial fertilizer at rates 0, 100, 200 kg N ha–1 or 200 kg N ha–1 plus starter P (40 kg ha–1) at time of planting. Whole-crop yield and N uptake were determined at harvest stage. Over two years, corn yielded more with greater N and P uptake after long-term applications of WS than LF, Fert and WS/Fert, regardless of current fertilizer applications. Additional mineral fertilizer N and P did not substitute for the historical effects. There was a significant linear relationship of corn N uptake with historical applications of organic-N but not with mineral-N or total-N across current fertilizer treatments. These results support farmer practice of supplementing first-year corn, after long-term manure application on grassland, with modest N and P as starter application only and then increasing N rates thereafter. [ABSTRACT FROM AUTHOR]

Carabid populations were assessed in relation to type and level of nutrient input in a multi-year trial on tall fescue, Festuca arundinacea. Thirteen species of carabids were recovered, Pterostichus melanarius representing 81% of all catches. In 3 m wide strip plots populations of P. melanarius increased with the rate of dairy slurry application, and the effect persisted for 2 years after applications ceased. The numerical response of P. melanarius to dairy slurry required several months, with other carabid species being largely unaffected by the treatments. Species diversity decreased with the number of years of dairy slurry application and with increasing application rates, and the effect persisted for 2 years. The decline in diversity was usually caused by changes in numbers of P. melanarius. Chemical fertilizer had no effect on carabid populations compared to controls. Earthworm biomass increased with increasing dairy slurry rate. The predatory capacity of the carabids increased with increasing dairy slurry rate, and the proportion of onion maggot pupae, Delia antiqua, consumed increased with P. melanarius numbers. The application of dairy slurry may therefore be used to improve pest control in forage grass. [Copyright &y& Elsevier]