1. High-purity propionate production from glycerol in mixed culture fermentation.
- Author
-
Chen Y, Wang T, Shen N, Zhang F, and Zeng RJ
- Subjects
- Acetates metabolism, Ammonium Compounds, Microbial Consortia, Bioreactors microbiology, Fermentation, Glycerol metabolism, Propionates metabolism
- Abstract
High-purity propionate production from glycerol in mixed culture fermentation (MCF) induced by high ammonium concentration was investigated. Fed-batch experiments revealed that higher ammonium concentration (>2.9g/L) had simultaneous negative effects on acetate and propionate degradation. Propionate production and yield was up to 22.6g/L and 0.45g COD/g COD glycerol, respectively, with a purity of 96%. Sequential batch experiments demonstrated that the yields of propionate were 0.3±0.05, 0.32±0.01, and 0.34±0.03g COD/g COD at a glycerol concentration of 2.78, 4.38, and 5.56g/L, respectively, and the purity of propionate was 91-100%. Microbial community analysis showed that the phylum Firmicutes dominated the bacterial community at different glycerol concentrations. However, the Methanosaeta population decreased from 46% to 6% when glycerol concentration increased from 2.78 to 5.56g/L, resulting in lower acetate degradation rate. Thus, the present study might provide an alternative option for the production of propionate from glycerol via MCF., (Copyright © 2016 Elsevier Ltd. All rights reserved.)
- Published
- 2016
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