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3 results on '"Palfi G"'

1. Application of sensors for the determination of physical and biochemical parameters in the fermentation of animal cells.

Author

Merten OW and Palfi GE

Subjects
Adenosine Triphosphate analysis, Amino Acids analysis, Animals, Culture Media analysis, DNA analysis, Electrodes, Flow Cytometry instrumentation, Glucose analysis, Immunologic Techniques, Lactates analysis, Pyruvates analysis, Biotechnology instrumentation, Cells, Cultured metabolism, Fermentation
Abstract

Biosensors, sondes, and transducer elements are reviewed with respect to the application for fermentation processes of animal cells. Hereby, the possible connection of these elements to the fermentor is shown. Specially, the (possible) online determinations of the viable and total cell count, of the glucose, the lactate, the pyruvate, and the ammonia concentration in the medium of fermentors are presented. Finally, some sensors for the on-line estimation of the product concentration are described.

Published
1987

3. A laboratory fermentor for agarose immobilized hybridomas to produce monoclonal antibodies

Author

Himmler G, Palfi G, Rüker F, Katinger H, and Scheirer W

Subjects
Mice, Hybridomas, Sepharose, Cytological Techniques, Fermentation, Animals, Antibodies, Monoclonal, Humans, Cell Division, Microspheres
Abstract

Mass culture of immobilized cells in airlift-fermenters usually ends up with the beads accumulating in the foamy layer on the surface of the reactor fluid or, in stirred tankreactors, with partial destruction of the beads. We tried to use an airlift fermenter vessel for growing cells, immobilized in agarose beads. Instead of using the gas for driving, we mounted a slowly turning marine type impeller within the drought tube. Oxygen was supplied on occasional demands by the original sparger. This set up leads to sufficient operational characteristics of the reactor without accumulation of the beads in the foamy layer and without mechanical destruction. Different productivities of either immobilized cells or cells in free suspension culture are reported.

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