Search

Biofuel production from lignocellulose feedstocks is sustainable and environmentally friendly. However, the lignocellulosic pretreatment could produce fermentation inhibitors causing multiple stresses and low yield. Therefore, the engineering construction of highly resistant microorganisms is greatly significant. In this study, a composite functional chimeric cellulosome equipped with laccase, versatile peroxidase, and lytic polysaccharide monooxygenase was riveted on the surface of Saccharomyces cerevisiae to construct a novel yeast strain YI/LVP for synergistic lignin degradation and cellulosic ethanol production. The assembly of cellulosome was assayed by immunofluorescence microscopy and flow cytometry. During the whole process of fermentation, the maximum ethanol concentration and cellulose conversion of engineering strain YI/LVP reached 8.68 g/L and 83.41%, respectively. The results proved the availability of artificial chimeric cellulosome containing lignin-degradation enzymes for cellulosic ethanol production. The purpose of the study was to improve the inhibitor tolerance and fermentation performance of S. cerevisiae through the construction and optimization of a synergistic lignin-degrading enzyme system based on cellulosome., (© 2024. The Author(s), under exclusive licence to Springer Nature B.V.)

High-solids simultaneous saccharification and fermentation integrating single-stage anaerobic digestion was designed for bioenergy conversion from steam-exploded Pennisetum purpereum , a perennial C 4 grass. Three scenarios for ethanol production, methane production and ethanol-methane co-production were investigated and compared in terms of production yield. A terminal ethanol titer of 62.6 ± 1.9 g/L with the highest ethanol yield of 90.9% was achieved when the fed-batch mode was carried out at a final substrate loading of 35% (w/v) dry matter content. The ethanol stillage was used for methane production in a semi-continuous CSTR system operated with different organic loading rates (OLRs) at a hydraulic retention time (HRT) of 30 days. The high performance was achieved at OLR of 6.0 g VS·L −1  d −1 , average yielding 358.7L CH 4 per kilogram volatile solid (VS) with a total VS removal rate of 73.8%. On the basis of mass balance, the overall production yield achieved in this study was: 121.6 g ethanol + 110.6 g methane per kilogram grass with a finally 89.6% of cellulose recovery + 62.5% hemicellulose recovery. These results indicated that the high-solids ethanol fermentation with single-stage methane anaerobic digestion of lignocellulosic biomass is a valid approach to maximize the holocellulose bioconversion and improve the bioenergy production. [ABSTRACT FROM AUTHOR]