Search

Your search keyword '"nicolas glaser"' showing total 8 results
Start Over Author "nicolas glaser" Topic female
8 results on '"nicolas glaser"'

1. Patient radiation dose in percutaneous biliary interventions: recommendations for DRLs on the basis of a multicentre study

Author

Erik-Sebastian Fuchs, Joachim Böttcher, Marko Weber, Jochen Rudi, Josef Tacke, Thomas Leimbach, Hans-Björn Gehl, Lars Höpner, Nour-Eldin A. Nour-Eldin, Andreas H. Mahnken, Nicolas Glaser, Harald Ittrich, Daniel Schmitz, Bernd Plessow, Andrea Tal, Ulrich Rosien, Svetlana Hetjens, Stefan Gölder, Wolfgang Schorr, Michael Böhmig, Jens-Christian Kröger, Markus Joerdens, Markus Wispler, Alexander Schegerer, Thomas J. Vogl, Bettina Friesenhahn-Ochs, and Boris Radeleff

Subjects
Adult, Male, medicine.medical_specialty, Percutaneous, Psychological intervention, Radiology, Interventional, Radiation Dosage, Radiography, Interventional, 030218 nuclear medicine & medical imaging, 03 medical and health sciences, 0302 clinical medicine, Reference Values, Germany, medicine, Humans, Radiology, Nuclear Medicine and imaging, Biliary Tract, Retrospective Studies, medicine.diagnostic_test, Bile duct, business.industry, Radiation dose, Significant difference, Interventional radiology, General Medicine, Radiation Exposure, Radiation exposure, Biliary Tract Surgical Procedures, medicine.anatomical_structure, Dose area product, 030220 oncology & carcinogenesis, Fluoroscopy, Female, Stents, Radiology, business
Abstract

Percutaneous biliary interventions (PBIs) can be associated with a high patient radiation dose, which can be reduced when national diagnostic reference levels (DRLs) are kept in mind. The aim of this multicentre study was to investigate patient radiation exposure in different percutaneous biliary interventions, in order to recommend national DRLs. A questionnaire asking for the dose area product (DAP) and the fluoroscopy time (FT) in different PBIs with ultrasound- or fluoroscopy-guided bile duct punctures was sent to 200 advanced care hospitals. Recommended national DRLs are set at the 75th percentile of all DAPs. Twenty-three facilities (9 interventional radiology depts. and 14 gastroenterology depts.) returned the questionnaire (12%). Five hundred sixty-five PBIs with 19 different interventions were included in the analysis. DAPs (range 4–21,510 cGy·cm2) and FTs (range 0.07–180.33 min) varied substantially depending on the centre and type of PBI. The DAPs of initial PBIs were significantly (p

Published
2018

2. Reconstructing human pancreatic differentiation by mapping specific cell populations during development

Author

Cyrille Ramond, Nicolas Glaser, Claire Berthault, Jacqueline Ameri, Jeannette Schlichting Kirkegaard, Mattias Hansson, Christian Honoré, Henrik Semb, and Raphaël Scharfmann

Subjects
Pluripotent Stem Cells, QH301-705.5, Science, Pancreatic Ducts, Gene Expression Regulation, Developmental, Cell Differentiation, Acinar Cells, Fetus, Developmental Biology and Stem Cells, Humans, Medicine, Cell Lineage, Female, human, pancreas, Endocrine Cells, Biology (General), Transcriptome, development, Biomarkers, Cells, Cultured, Research Article
Abstract

Information remains scarce on human development compared to animal models. Here, we reconstructed human fetal pancreatic differentiation using cell surface markers. We demonstrate that at 7weeks of development, the glycoprotein 2 (GP2) marks a multipotent cell population that will differentiate into the acinar, ductal or endocrine lineages. Development towards the acinar lineage is paralleled by an increase in GP2 expression. Conversely, a subset of the GP2+ population undergoes endocrine differentiation by down-regulating GP2 and CD142 and turning on NEUROG3, a marker of endocrine differentiation. Endocrine maturation progresses by up-regulating SUSD2 and lowering ECAD levels. Finally, in vitro differentiation of pancreatic endocrine cells derived from human pluripotent stem cells mimics key in vivo events. Our work paves the way to extend our understanding of the origin of mature human pancreatic cell types and how such lineage decisions are regulated. DOI: http://dx.doi.org/10.7554/eLife.27564.001

Published
2017
Full Text
View/download PDF

3. Priming 3D Cultures of Human Mesenchymal Stromal Cells Toward Cartilage Formation Via Developmental Pathways

Author

Stefan Schären, Matteo Centola, Beatrice Tonnarelli, Nicolas Glaser, Ivan Martin, and Andrea Barbero

Subjects
Adult, Male, Stromal cell, Cellular differentiation, Cell Culture Techniques, Gene Expression, Bone Marrow Cells, Cartilage metabolism, Biology, Fibroblast growth factor, 03 medical and health sciences, Chondrocytes, 0302 clinical medicine, Original Research Reports, Wnt3A Protein, Humans, Limb development, Wnt Signaling Pathway, Cells, Cultured, Cell Proliferation, 030304 developmental biology, 0303 health sciences, Reverse Transcriptase Polymerase Chain Reaction, Mesenchymal stem cell, Cell Differentiation, Mesenchymal Stem Cells, Cell Biology, Hematology, Flow Cytometry, Molecular biology, 3. Good health, Cell biology, Cartilage, Microscopy, Fluorescence, Cell culture, 030220 oncology & carcinogenesis, Female, Fibroblast Growth Factor 2, Stem cell, Chondrogenesis, Signal Transduction, Developmental Biology
Abstract

The field of regenerative medicine has increasingly recognized the importance to be inspired by developmental processes to identify signaling pathways crucial for 3D organogenesis and tissue regeneration. Here, we aimed at recapitulating the first events occurring during limb development (ie, cell condensation and expansion of an undifferentiated mesenchymal cell population) to prime 3D cultures of human bone marrow-derived mesenchymal stromal/stem cells (hBM-MSC) toward the chondrogenic route. Based on embryonic development studies, we hypothesized that Wnt3a and fibroblast growth factor 2 (FGF2) induce hBM-MSC to proliferate in 3D culture as an undifferentiated pool of progenitors (defined by clonogenic capacity and expression of typical markers), retaining chondrogenic potential upon induction by suitable morphogens. hBM-MSC were responsive to Wnt signaling in 3D pellet culture, as assessed by significant upregulation of main target genes and increase of unphosphorylated β-catenin levels. Wnt3a was able to induce a five-fold increase in the number of proliferating hBM-MSC (6.4% vs. 1.3% in the vehicle condition), although total DNA content of the 3D construct was decreasing over time. Preconditioning with Wnt3a improved transforming growth factor-β1 mediated chondrogenesis (30% more glycosaminoglycans/cell in average). In contrast to developmental and 2D MSC culture models, FGF2 antagonized the Wnt-mediated effects. Interestingly, the CD146+ subpopulation was found to be more responsive to Wnt3a. The presented data indicate a possible strategy to prime 3D cultures of hBM-MSC by invoking a “developmental engineering” approach. The study also identifies some opportunities and challenges to cross-fertilize skeletal development models and 3D hBM-MSC culture systems.

Published
2013

4. Mutations in BOREALIN cause thyroid dysgenesis

Author

Athanasia Stoupa, Claude Besmond, Cyrille Ramond, Delphine Zenaty, Raphael Scharfmann, Christine Bole-Feysot, Dulanjalee Kariyawasam, William R. Taylor, Arnold Munnich, Sébastien Gaujoux, Laurence Hubert, Juliane Léger, Taylor M Monus, Patrick Nitschke, Nicolas Glaser, Frédéric Sebag, Aurore Carré, Michel Polak, Stanislas Lyonnet, and Manelle Gueriouz

Subjects
0301 basic medicine, medicine.medical_specialty, Mutation, Missense, Mitosis, Cell Cycle Proteins, Biology, medicine.disease_cause, Thyroid dysgenesis, 03 medical and health sciences, Cell Movement, Internal medicine, Genetics, medicine, Missense mutation, Humans, Exome, Genetic Predisposition to Disease, Molecular Biology, Gene, Genetics (clinical), Exome sequencing, Mutation, Thyroid, High-Throughput Nucleotide Sequencing, General Medicine, Articles, medicine.disease, Congenital hypothyroidism, Pedigree, 030104 developmental biology, Endocrinology, medicine.anatomical_structure, Gene Expression Regulation, Thyroid Dysgenesis, Female, PAX8
Abstract

Congenital hypothyroidism is the most common neonatal endocrine disorder and is primarily caused by developmental abnormalities otherwise known as thyroid dysgenesis (TD). We performed whole exome sequencing (WES) in a consanguineous family with TD and subsequently sequenced a cohort of 134 probands with TD to identify genetic factors predisposing to the disease. We identified the novel missense mutations p.S148F, p.R114Q and p.L177W in the BOREALIN gene in TD-affected families. Borealin is a major component of the Chromosomal Passenger Complex (CPC) with well-known functions in mitosis. Further analysis of the missense mutations showed no apparent effects on mitosis. In contrast, expression of the mutants in human thyrocytes resulted in defects in adhesion and migration with corresponding changes in gene expression suggesting others functions for this mitotic protein. These results were well correlated with the same gene expression pattern analysed in the thyroid tissue of the patient with BOREALIN-p.R114W. These studies open new avenues in the genetics of TD in humans.

Published
2016

5. Differential expression of the chemosensory transcriptome in two populations of the stemborer Sesamia nonagrioides

Author

Fabrice Legeai, Aurore Gallot, Emmanuelle Jacquin-Joly, Paul-André Calatayud, Bruno Le Ru, Myriam Harry, Nicolas Glaser, Laure Kaiser, Institut d'écologie et des sciences de l'environnement de Paris (iEES), Centre National de la Recherche Scientifique (CNRS)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Recherche Agronomique (INRA), Évolution, génomes, comportement et écologie (EGCE), Centre National de la Recherche Scientifique (CNRS)-IRD-Université Paris-Sud - Paris 11 (UP11), Institut de Génétique, Environnement et Protection des Plantes (IGEPP), Institut National de la Recherche Agronomique (INRA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Institut National de la Recherche Agronomique (INRA)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Centre National de la Recherche Scientifique (CNRS), Université Paris-Sud - Paris 11 (UP11)-IRD-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Université de Rennes (UR)-AGROCAMPUS OUEST, Institut d'écologie et des sciences de l'environnement de Paris (IEES), Scalable, Optimized and Parallel Algorithms for Genomics (GenScale), GESTION DES DONNÉES ET DE LA CONNAISSANCE (IRISA-D7), Institut de Recherche en Informatique et Systèmes Aléatoires (IRISA), Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Université de Bretagne Sud (UBS)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Rennes (ENS Rennes)-Institut National de Recherche en Informatique et en Automatique (Inria)-Télécom Bretagne-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-CentraleSupélec-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Université de Rennes (UNIV-RENNES)-CentraleSupélec-Institut de Recherche en Informatique et Systèmes Aléatoires (IRISA), Université de Rennes (UNIV-RENNES)-CentraleSupélec-Inria Rennes – Bretagne Atlantique, Institut National de Recherche en Informatique et en Automatique (Inria), Evolution, adaptation et comportement, Département écologie évolutive [LBBE], Laboratoire de Biométrie et Biologie Evolutive - UMR 5558 (LBBE), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Laboratoire de Biométrie et Biologie Evolutive - UMR 5558 (LBBE), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS), International Centre of Insect Physiology and Ecology (ICIPE), and ICIPE

Subjects
Arthropod Antennae, Oviposition, media_common.quotation_subject, [SDV]Life Sciences [q-bio], Population, Sesamia nonagrioides, Genes, Insect, Insect, Moths, Typhaceae, Zea mays, Biochemistry, Transcriptome, Cytochrome P-450 Enzyme System, medicine, Animals, Adaptation, education, Molecular Biology, media_common, Local adaptation, education.field_of_study, Olfactory receptor, biology, Ecology, Gene Expression Profiling, fungi, biology.organism_classification, RNAseq, Adaptation, Physiological, Kenya, Smell, Gene expression profiling, medicine.anatomical_structure, Evolutionary biology, Larva, Insect Science, Female, Chemosensory genes, France, [INFO.INFO-BI]Computer Science [cs]/Bioinformatics [q-bio.QM]
Abstract

International audience; Among the proposed mechanisms of local adaptation to different ecological environments, transcriptional changes may play an important role. In this study, we investigated whether such variability occurred within the chemosensory organs of a herbivorous insect, for which chemosensation guides most of its host preferences. A European and an African population of the noctuid Sesamia nonagrioides that display significant differences in their ecological preferences were collected on Zea mays and Typha domingensis, respectively. RNAseq were used between the two populations for digital expression profiling of chemosensory organs from larval antennae and palps. Preliminary data on adult female antennae and ovipositors were also collected. We found 6,550 differentially expressed transcripts in larval antennae and palps. Gene ontology enrichment analyses suggested that transcriptional activity was overrepresented in the French population and that virus and defense activities were overrepresented in the Kenyan population. In addition, we found differential expression of a variety of cytochrome P450s, which may be linked to the different host-plant diets. Looking at olfactory genes, we observed differential expression of numerous candidate odorant-binding proteins, chemosensory proteins, and one olfactory receptor, suggesting that differences in olfactory sensitivity participate in insect adaptation.

Published
2015
Full Text
View/download PDF

6. Similar Differentiation Patterns Between PBP Expression Levels and Pheromone Component Ratios in Two Populations of Sesamia nonagrioides

Author

Thomas Lecocq, Ene Leppik, Myriam Harry, Christelle Monsempes, Claire Capdevielle-Dulac, Brigitte Frérot, Bruno Le Ru, Nicolas Glaser, Emmanuelle Jacquin-Joly, Paul-André Calatayud, Physiologie de l'Insecte, Signalisation et Communication [Versailles] (PISC), Institut National de la Recherche Agronomique (INRA), Institut d'écologie et des sciences de l'environnement de Paris (iEES), Centre National de la Recherche Scientifique (CNRS)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Recherche Agronomique (INRA), Physiologie de l'Insecte : Signalisation et Communication (PISC), Institut National de la Recherche Agronomique (INRA)-Université Pierre et Marie Curie - Paris 6 (UPMC)-AgroParisTech, Université Paris Sud (Paris 11), UMR Lab Evolut. Genomes Speciat., Institut de Recherche pour le Développement (IRD [France-Ouest]), Unité de Recherches Animal et Fonctionnalités des Produits Animaux (URAFPA), Université de Lorraine (UL)-Institut National de la Recherche Agronomique (INRA), Laboratoire de Zoologie [Mons], University of Mons [Belgium] (UMONS), Évolution, génomes, comportement et écologie (EGCE), Centre National de la Recherche Scientifique (CNRS)-IRD-Université Paris-Sud - Paris 11 (UP11), French ANR (Agence Nationale de la Recherche) [ANR-09-PEXT-009], Institut d'écologie et des sciences de l'environnement de Paris (IEES), Institut National de la Recherche Agronomique (INRA)-Université de Lorraine (UL), Université Paris-Sud - Paris 11 (UP11)-IRD-Centre National de la Recherche Scientifique (CNRS), and Institut National de la Recherche Agronomique (INRA)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Male, Sex pheromones, Pheromone-binding proteins, Population, Sesamia nonagrioides, Gene Expression, Moths, Real-Time Polymerase Chain Reaction, Biochemistry, Lepidoptera genitalia, Quantitative PCR, Stemborer, Botany, polycyclic compounds, Animals, Differential expression, Sex Attractants, education, Ecology, Evolution, Behavior and Systematics, Phylogeny, Genetics, education.field_of_study, biology, Geography, General Medicine, biology.organism_classification, Kenya, Lepidoptera, [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, Noctuidae, Sex pheromone, Pheromone, Insect Proteins, Female, France, Carrier Proteins
Abstract

International audience; Pheromone-binding proteins (PBPs) are thought to contribute to the specificity of the pheromone detection system through an initial selective binding with pheromone molecules. Here, we report different expression levels of PBP transcripts in the antennae of two populations of the stemborer Sesamia nonagrioides (Lepidoptera: Noctuidae), one collected in Europe and one in sub-Saharan Africa. The three PBP transcripts previously identified in this species were found to be expressed in both male and female antennae. Whereas PBP3 did not show any differential expression, PBP1 and PBP2 appeared to be expressed differently according to the population origin and sex. Simultaneously, we measured and compared the ratio of the three components of the S. nonagrioides pheromone blend (Z11-16:Ac; Z11-16:OH; Z11-16:Ald) in females of the two populations. The ratio of Z11-16:OH and Z11-16:Ald varied significantly according to the population origin of this species. Cluster analyses revealed similar differentiation patterns between PBP1 and PBP2 expression levels and the ratios of Z11-16:OH and Z11-16:Ald. Different female sexual signals may thus correspond to different male reception systems, which are adjusted by the PBP expression levels, thereby ensuring optimal communication within populations.

Published
2014
Full Text
View/download PDF

7. A Comparison of the Olfactory Gene Repertoires of Adults and Larvae in the Noctuid Moth Spodoptera littoralis

Author

Nicolas Montagné, Fabrice Legeai, Erwan Poivet, Aurore Gallot, Emmanuelle Jacquin-Joly, Nicolas Glaser, Physiologie de l'Insecte : Signalisation et Communication (PISC), Institut National de la Recherche Agronomique (INRA)-Université Pierre et Marie Curie - Paris 6 (UPMC)-AgroParisTech, Evolution, adaptation et comportement, Département écologie évolutive [LBBE], Laboratoire de Biométrie et Biologie Evolutive - UMR 5558 (LBBE), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Laboratoire de Biométrie et Biologie Evolutive - UMR 5558 (LBBE), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Navier (navier umr 8205), Institut Français des Sciences et Technologies des Transports, de l'Aménagement et des Réseaux (IFSTTAR)-École des Ponts ParisTech (ENPC)-Centre National de la Recherche Scientifique (CNRS), Physiologie de l'Insecte, Signalisation et Communication [Versailles] (PISC), Institut National de la Recherche Agronomique (INRA), Institut de Génétique, Environnement et Protection des Plantes (IGEPP), Institut National de la Recherche Agronomique (INRA)-Université de Rennes (UR)-AGROCAMPUS OUEST, Institut National de la Recherche Agronomique (INRA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Scalable, Optimized and Parallel Algorithms for Genomics (GenScale), GESTION DES DONNÉES ET DE LA CONNAISSANCE (IRISA-D7), Institut de Recherche en Informatique et Systèmes Aléatoires (IRISA), CentraleSupélec-Télécom Bretagne-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de Recherche en Informatique et en Automatique (Inria)-École normale supérieure - Rennes (ENS Rennes)-Université de Bretagne Sud (UBS)-Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-CentraleSupélec-Télécom Bretagne-Université de Rennes 1 (UR1), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-Institut de Recherche en Informatique et Systèmes Aléatoires (IRISA), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-Inria Rennes – Bretagne Atlantique, Institut National de Recherche en Informatique et en Automatique (Inria), AGROCAMPUS OUEST-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de la Recherche Agronomique (INRA), Inria Rennes – Bretagne Atlantique, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria)-GESTION DES DONNÉES ET DE LA CONNAISSANCE (IRISA-D7), Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université de Bretagne Sud (UBS)-École normale supérieure - Rennes (ENS Rennes)-Institut National de Recherche en Informatique et en Automatique (Inria)-Télécom Bretagne-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS)-Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université de Bretagne Sud (UBS)-École normale supérieure - Rennes (ENS Rennes)-Institut National de Recherche en Informatique et en Automatique (Inria)-Télécom Bretagne-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche en Informatique et Systèmes Aléatoires (IRISA), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université de Bretagne Sud (UBS)-École normale supérieure - Rennes (ENS Rennes)-Télécom Bretagne-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-Université de Bretagne Sud (UBS)-École normale supérieure - Rennes (ENS Rennes)-Institut National de Recherche en Informatique et en Automatique (Inria)-Télécom Bretagne-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-Université de Bretagne Sud (UBS)-École normale supérieure - Rennes (ENS Rennes)-Institut National de Recherche en Informatique et en Automatique (Inria)-Télécom Bretagne-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche en Informatique et Systèmes Aléatoires (IRISA), and Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-Université de Bretagne Sud (UBS)-École normale supérieure - Rennes (ENS Rennes)-Télécom Bretagne-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS)

Subjects
Male, Gene Expression, Genes, Insect, Insect, anophèles gambiae, Transcriptomes, Transcriptome, 0302 clinical medicine, Morphogenesis, Cluster Analysis, media_common, Genetics, Heliothis-Virescens, 0303 health sciences, Larva, Multidisciplinary, Ecology, Odorant-Binding-Protein, Manduca-Sexta, Genomics, Multiple Sequence Alignment, [SDV.BIBS]Life Sciences [q-bio]/Quantitative Methods [q-bio.QM], Sensory Systems, Smell, Medicine, Pheromone, Female, récepteur, Research Article, phéromone sexuelle, Arthropod Antennae, Science, media_common.quotation_subject, Chemosensory Proteins, Spodoptera, Biology, Sex-Pheromone Receptor, bombyx mori, 03 medical and health sciences, Genome Analysis Tools, Botany, Animals, Caterpillar, Spodoptera littoralis, Ionotropic Glutamate Receptors, Gene, 030304 developmental biology, Mosquito Anopheles-Gambiae, Olfactory System, Chemical Ecology, Expression Pattern, Bombyx-Mori, Gene Expression Profiling, fungi, Computational Biology, Molecular Sequence Annotation, Olfactory Perception, biology.organism_classification, heliothis virescens, Gene expression profiling, [INFO.INFO-BI]Computer Science [cs]/Bioinformatics [q-bio.QM], [SDE.BE]Environmental Sciences/Biodiversity and Ecology, Zoology, Entomology, 030217 neurology & neurosurgery, Developmental Biology, Neuroscience
Abstract

International audience; To better understand the olfactory mechanisms in a lepidopteran pest model species, the cotton leafworm Spodoptera littoralis, we have recently established a partial transcriptome from adult antennae. Here, we completed this transcriptome using next generation sequencing technologies, namely 454 and Illumina, on both adult antennae and larval tissues, including caterpillar antennae and maxillary palps. All sequences were assembled in 77,643 contigs. Their analysis greatly enriched the repertoire of chemosensory genes in this species, with a total of 57 candidate odorant-binding and chemosensory proteins, 47 olfactory receptors, 6 gustatory receptors and 17 ionotropic receptors. Using RT-PCR, we conducted the first exhaustive comparison of olfactory gene expression between larvae and adults in a lepidopteran species. All the 127 candidate olfactory genes were profiled for expression in male and female adult antennae and in caterpillar antennae and maxillary palps. We found that caterpillars expressed a smaller set of olfactory genes than adults, with a large overlap between these two developmental stages. Two binding proteins appeared to be larvae-specific and two others were adult-specific. Interestingly, comparison between caterpillar antennae and maxillary palps revealed numerous organ-specific transcripts, suggesting the complementary involvement of these two organs in larval chemosensory detection. Adult males and females shared the same set of olfactory transcripts, except two male-specific candidate pheromone receptors, two male-specific and two female-specific odorant-binding proteins. This study identified transcripts that may be important for sex-specific or developmental stage-specific chemosensory behaviors.

Published
2013
Full Text
View/download PDF

8. The use of the sex pheromone as an evolutionary solution to food source selection in caterpillars

Author

Didier Rochat, Emmanuelle Jacquin-Joly, Frédéric Marion-Poll, Nicolas Glaser, Kacem Rharrabe, Michel Renou, Christelle Monsempes, Erwan Poivet, Physiologie de l'Insecte, Signalisation et Communication [Versailles] (PISC), and Institut National de la Recherche Agronomique (INRA)

Subjects
Arthropod Antennae, Male, 0106 biological sciences, animal structures, [SDV]Life Sciences [q-bio], General Physics and Astronomy, Zoology, Spodoptera, 01 natural sciences, General Biochemistry, Genetics and Molecular Biology, Food Preferences, 03 medical and health sciences, pheromone, Botany, Animals, sex, Sensilla, Sex Attractants, Spodoptera littoralis, Caterpillar, 030304 developmental biology, 0303 health sciences, Larva, Multidisciplinary, biology, fungi, General Chemistry, caterpillars, biology.organism_classification, Biological Evolution, 010602 entomology, Sex pheromone, Insect Proteins, Pheromone, Female, sense organs
Abstract

Sex pheromones are released by adults of a species to elicit a sexual interaction with the other sex of the same species. Here we report an unexpected effect of a moth sex pheromone on the caterpillars of the same species. We demonstrate that larvae of the cotton leafworm Spodoptera littoralis are attracted by the moth sex pheromone and that this phenomenon is independent of sex determination. In addition, we show that the olfactory sensilla carried by the caterpillar antennae are sensitive to the pheromone and that the caterpillar sensilla express pheromone-binding proteins that are used by adult antennae to bind pheromone components. Finally, we demonstrate that the larvae are preferentially attracted to a food source when it contains the sex pheromone main component. A possible interpretation of these results is that the sex pheromone is used to promote food search in caterpillars, opening potential new routes for insect pest management.

Published
2012
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results