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The combination of 8-methoxypsoralen and near UV light is highly effective in inducing sister chromatid exchanges (SCEs) in Chinese hamster ovary cells. Appreciable increases in SCEs can be effected by treatments compatible with cell survival, and effects of a single dose of alkylation persist over multiple generations. Both the frequency and location of SCEs induced at different times within the DNA synthesis period varies in a manner indicating that exchange induction is restricted to regions which replicated during or after DNA damage.

A woman with primary amenorrhea and pure gonadal dysgenesis had two cytogenetically abnormal cell lines. The karyotype was 45,X in 56--95% of mitosis from lymphocytes and skin fibroblasts. In the remaining 5--44% of the cells there was, in addition to a normal X, a structurally abnormal X chromosome interpretable as pter leads to q21::q11 leads to pter or pter leads to q21::q13 leads to pter. The abnormal X chromosome was heterocyclic and had a normal centromere plus an extra C band in the long arm. Detailed interpretation of the structural rearrangements of this chromosome required the use of both Q-, G-, and C-banding and the BrdU-Hoechst 33258 technique.