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2 results on '"Munetoshi Katagiri"'

1. The Function and Prognostic Significance of Cripto-1 in Colorectal Cancer

Author

Munetoshi Katagiri, Takeshi Naitoh, Hideaki Karasawa, Jun Sato, Michiaki Unno, Shimpei Maeda, Shinobu Ohnuma, Fuyuhiko Motoi, Shun Nakayama, and Takashi Suzuki

Subjects
Adult, Male, 0301 basic medicine, Cancer Research, Colorectal cancer, Kaplan-Meier Estimate, GPI-Linked Proteins, Cripto, Disease-Free Survival, Mice, 03 medical and health sciences, 0302 clinical medicine, Cell Line, Tumor, Biomarkers, Tumor, medicine, Animals, Humans, RNA, Small Interfering, Aged, Aged, 80 and over, Plasma membrane protein, business.industry, General Medicine, Middle Aged, Prognosis, medicine.disease, Xenograft Model Antitumor Assays, Neoplasm Proteins, 030104 developmental biology, Oncology, Biological significance, Gene Knockdown Techniques, 030220 oncology & carcinogenesis, Cancer research, Intercellular Signaling Peptides and Proteins, Immunohistochemistry, Female, Colorectal Neoplasms, business, hormones, hormone substitutes, and hormone antagonists, Function (biology), Follow-Up Studies
Abstract

Cripto-1 is a plasma membrane protein which is not expressed in adult tissue, but some tumors are accompanied by re-activation. We studied the clinical and biological significance of Cripto-1 in colorectal cancer. Cripto-1 was positive in 68 out of 192 cases (35%) by immunohistochemistry. Cripto-1 expression was correlated with worse prognosis and was an independent prognostic factor. Cripto-1-silenced colorectal cancer cell lines had reduced cell proliferation, migration, and activation of Akt and MAPK signaling pathways

Published
2020
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2. Hexokinase 2 in colorectal cancer: a potent prognostic factor associated with glycolysis, proliferation and migration

Author

Munetoshi, Katagiri, Hideaki, Karasawa, Kiyoshi, Takagi, Shun, Nakayama, Shinichi, Yabuuchi, Fumiyoshi, Fujishima, Takeshi, Naitoh, Mika, Watanabe, Takashi, Suzuki, Michiaki, Unno, and Hironobu, Sasano

Subjects
Adult, Male, Kaplan-Meier Estimate, Adenocarcinoma, Middle Aged, Prognosis, Real-Time Polymerase Chain Reaction, Immunohistochemistry, Cell Movement, Cell Line, Tumor, Gene Knockdown Techniques, Hexokinase, Humans, Female, Colorectal Neoplasms, Glycolysis, Aged, Cell Proliferation, Proportional Hazards Models
Abstract

It is well known that proliferating carcinoma cells preferentially use aerobic glycolysis rather than oxidative phosphorylation for energy production. Hexokinase 2 (HK2) plays a pivotal role in the glycolytic pathway. Previous studies have demonstrated that HK2 activity is markedly increased in various malignant neoplasms, but the clinical and biological significance of HK2 remain largely unclear in the colorectal carcinoma.We performed immunohistochemistry for HK2 in 195 colorectal carcinoma tissues. We also used HCT8 and HT29 colon carcinoma cells in in vitro studies.HK2 immunoreactivity was detected in 100 out of 195 (51%) colorectal carcinoma tissues, and the immunohistochemical HK2 status was significantly associated with tumor size, depth of invasion, liver metastasis and TNM stage in these cases. Moreover, the HK2 status was significantly associated with increased incidence of recurrence and overall mortality of the patients, and multivariate analyses demonstrated that HK2 status was an independent prognostic factor for both disease-free and overall survival. Subsequent in vitro experiments revealed that both HCT8 and HT29 colon carcinoma cells transfected with specific siRNA for HK2 significantly decreased the lactate production, proliferation activity and migration property.These results suggest that HK2 plays important roles in the glycolytic, proliferation and migration properties of colorectal carcinoma and, therefore, HK2 status is a potent worse prognostic factor in colorectal cancer patients.

Published
2016

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