1. Cytolytic Activity (CYT) Score Is a Prognostic Biomarker Reflecting Host Immune Status in Hepatocellular Carcinoma (HCC)
- Author
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Hiroaki Wakiyama, Taro Tobo, Hidetoshi Eguchi, Koshi Mimori, Masakazu Hirakawa, Katsumi Sakamoto, Hiroshi Honda, Qingjiang Hu, Takaaki Masuda, and Yushi Motomura
- Subjects
Adult ,Cytotoxicity, Immunologic ,Male ,0301 basic medicine ,Oncology ,Cancer Research ,medicine.medical_specialty ,Carcinoma, Hepatocellular ,environment and public health ,Cohort Studies ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,Biomarkers, Tumor ,Tumor Cells, Cultured ,Humans ,Medicine ,Clinical significance ,Prognostic biomarker ,Aged ,Aged, 80 and over ,Immune status ,business.industry ,Liver Neoplasms ,General Medicine ,Middle Aged ,Prognosis ,medicine.disease ,Peripheral blood ,enzymes and coenzymes (carbohydrates) ,Cytolysis ,Immunity, Active ,030104 developmental biology ,Research Design ,030220 oncology & carcinogenesis ,Hepatocellular carcinoma ,embryonic structures ,cardiovascular system ,Biomarker (medicine) ,Immunohistochemistry ,Female ,business - Abstract
Background/aim The cytolytic activity (CYT) score is a new index of cancer immunity calculated from the mRNA expression levels of GZMA and PRF1. We assessed the clinical significance of the CYT score in HCC. Materials and methods The calculated CYT scores of peripheral blood cells (GSE24759), cell lines (CCLE) and HCC tissues (TCGA, GSE14520 and Kyushu cohorts) were assessed. Then, immunohistochemical analysis (IHC) of GZMA and PRF1 was performed. Results The CYT scores of HCC tissues were lower than those of non-cancerous tissues. The 5-year recurrence-free survival of patients with low CYT scores was significantly shorter than that of patients with high CYT scores. Multivariate analysis indicated that the CYT score was an independent prognostic factor for RFS in TCGA and GSE14520 cohorts. Conclusion CYT score could be a useful prognostic biomarker in HCC, possibly through reflecting the host immune status.
- Published
- 2018