Search

We studied functional activity of neutrophilic granulocytes and monocytes isolated from the peripheral blood of women during the follicular and luteal phases of the menstrual cycle. It was shown that phagocytic activity of neutrophilic granulocytes increases, their intracellular oxygen-dependent bactericidal activity decreases, and the number of monocyte extracellular traps increases in women in the luteal phase of the menstrual cycle in comparison with the follicular phase.

Study effects of microwave electromagnetic radiation (EMR) of the SHF range in vitro on viability and functional status of neutrophilic granulocytes.Neutrophils of peripheral blood of 30 conditionally healthy donors aged 18 - 20 years were exposed to the effect of broadband (natural, similar to sun radio emission) and, mono-frequency (technogenic, similar to emission of cell phones, PCs, microwave ovens, etc.) EMR with frequency range of 4 - 4.34 GHz, generated by a SHF-generatorAIMT-1. 16 minutes after the effect, viability of neutrophilic granulocytes, phagocytic activity, lysosome activity ofcells were studied, NBT-reducing ability and cytokine content in supernatant were evaluated.The amount of viable neu- trophils significantly reduced after the effect of modelled EMR with technogenic frequency- temporal structure, functional status of viable cells did not change. Neutrophils retained viability after the effect of EMR of natural origin, increased levels of IL1β and TNFα was determined in supernatants, functional status of neutrophils of men remained stable, and in women an increase of phagocytic ability and a reduction of production of oxygen radicals was noted.The detected reduction of the amount of viable neutrophils under the effect of EMR of technogenic origin could result in disturbances in the system of innate immunity, other homeostasis elements of the human organism and development of pathologic conditions. At the same time, the de- tected effects of EMR of natural origin. open perspectives of use of modelled microwave EMR of SHF range in prophylaxis and clinical medicine.

Evaluation of microbiological effectiveness of longidaze preparation use during therapy of active non-specific endometritis in women with uterus myoma.2 groups of women were formed by a method of random selection. The first was composed of patients with active chronic endometritis, that had received standard etiotropic antibacterial therapy. The second included patients, that had received longidaze in addition to the standard therapy. Therapy effectiveness evaluation was carried out based on the analysis of microbial landscape of cervical canal and uterine cavity 2 months after the therapy during phase I of the menstrual cycle.Non-plasma-coagulating staphylococci, enterococci and anaerobes were established to be present predominately in the examined women of both the first and the second group.The application of longidaze in combination with antibacterial therapy results in the most significant improvement of microbial landscape of cervical canal and uterine cavity.

The effects of phorbol-12-myristate-13-acetate on functional activity and viability of neutrophil granulocytes in the saliva of women at various phases of menstrual cycle were studied using NBT-test and method for detection of neutrophil traps in mucosa secretions. The activator increased the number of neutrophil traps and parameters of neutrophil NBT-test.

It is established that in patients with chronic pyelonephritis of single kidney the antibacterial defense of organism and balance between pro- and antioxidant systems are disturbed both in active and latent stages of disease. The results testify the need of organization of comprehensive immunologic and biochemical examination to establish a prognosis of complications and evaluation of effectiveness of applied treatment.

To study the influence of Solcotichovac vaccine on formation of neutrophil extracellular traps.Solcotrichovac vaccine administered in various doses was used in order to assess the formation of neutrophil extracellular traps in peripheral blood of women of childbearing age.Therapeutic dose of Solcotrichovac stimulated the most prominent formation of neutrophil extracellular traps.Solcotrichovac vaccine stimulated non-specific arm of mucosal immunity due to formation of neutrophil extracellular traps and it is probable that this mechanism explains the effect of this vaccine.

Effect of low-intensity laser radiation on the main effector functions of neutrophils from the cervical discharge was studied in women with mycoplasma infection and dysfunction of neutrophil granulocytes manifest in the first place as a reduced number of phagocytic cells and decreased intensity of phagocytosis. The fraction of intracellular active forms of oxygen in cervical neutrophils from infected women was smaller than in healthy ones. Irradiation by low-intensity laser light increased both activity of pagocytosis and intracellular generation of active oxygen forms. It is concluded that low-intensity laser radiation normalizes disturbed neutrophil function.

The influence of vaginal liquid on adhesive reactions in the system "Candida albicans - vaginal epitheliocytes". Preliminary treatment of C. albicans by mucin, natural or absorbed vaginal liquid resulted in decrease of adhesive activity (p0.05). C. albicans did not change its adhesive properties in case of preliminary treatment of vaginal epitheliocytes by the same substances. In the system "Candida albicans - vaginal epitheliocytes" vaginal liquid has the expressed antiadhesive effect, which probably belongs to mucin.

Reactive anxiety, immune system function, and metabolism were studied in healthy volunteers. Temperament characteristics, state of health, number of leukocytes and platelets, and concentration of glucose, albumin, and fibrinogen significantly differed in subjects with low, moderate, and high level of reactive anxiety.

Low-intensity laser impact on the main effector functions of the cervical secretion neutrophils in females suffering from Chlamidia infection has been studied. Dysfunction of neutrophil granulocytes of the cervical secretion was revealed. This manifested in the decreased number of phagocytes and lower rates of phagocytosis. The study of cervical secretion neutrophils has found that the content of active intracellular forms of oxygen was decreased in patients compared to healthy subjects. Low-intensity laser stimulated not only phagocytosis but also intracellular generation of active oxygen forms. Thus, low-intensity laser eliminates dysfunction of cervical secretion neutrophils.

The influence of neutrophils and their secretory products on the microflora of the vaginal contents in healthy women and in women having dysbiotic processes in the vagina was studied. The secretory products of neutrophils were found to produce a bactericidal effect on the representatives of the opportunistic bacteria, this effect being less pronounced with respect to lactic-acid bacteria. The established effect of neutrophils on bacteria is regarded as one of the mechanisms of microbiocenosis formation, ensuring colonization resistance.

In cervical secretions of healthy non-pregnant women of the reproductive age high concentrations of ant-inflammatory cytokines, greatly exceeding those in blood serum, were detected. During pregnancy the level of TNF-alpha in cervical secretions dropped. The inflammation of the uterus neck was accompanied by a drop in the levels of IL-alpha and IL-1beta and a rise in the level of IL-8 in cervical secretions in pregnant and non-pregnant women. Similar changes in the cytokine profile occurred also in the blood serum, but they were less pronounced and could be observed only in non-pregnant women. The threat of the interruption of pregnancy, developing simultaneously with cervicitis, was accompanied not only by changes in the levels of cytokines in cervical secretions, but also by a perceptible increase in the content of IL-1alpha in the blood serum.

The functional activity of neutrophils, peroxidation products and antioxidative activity of cervical secretion in women with the acute inflammatory process in the upper section of the reproductive tract were under study. The most pronounced changes in the function of neutrophils were observed in women with the low antioxidative activity of cervical slime.

A total of 52 women with established presence of the causative agent of chlamydial infection in the cervical canal were examined. The chlamydial lesion of the cervix uteri was characterized by the absence of clinical manifestations in 25% of cases, faintly pronounced local inflammatory process in the lower part of the genitals in the form of chronic cervititis in 57.7% of cases and the symptoms of subacute cervicitis in 17.3% of cases. In case of a chronic course of chlamydial cervicitis a low content of cytokines IL-1alpha, IL-1beta, TNF-alpha and an elevated concentration of IL-8 were observed. In the cervical slime of patients with chlamydial cervicitis no changes in the total content of leukocytes were noted in comparison with healthy women, but the number of nitro blue tetrazoliu reducing neutrophils increased, while their functional reserve and phagocytic activity dropped. Autoneutrophilokins used for local immunocorrection in the treatment of women with chlamydial cervicitis produced a normalizing effect on the local infectious protective factors of cervical secretion, thus enhancing the effectiveness of therapy.

A retrospective analysis of medical histories with acute lymphoblast leucosis and non-B-cell non-Hodgkin lymphomas made it possible to reveal infectious complications of cytostatic therapy in 100% of children, namely: sepsis (0.3%), unidentified infection (12%), local infection (87.7%). Infectious complications of the cytopenic nature were localized mainly in the upper sections of the gastrointestinal tract and in upper respiratory tract. Bacterial infectious complications caused by opportunistic microorganisms with the prevalence of Gram positive flora, resistant to cephalosporins of generations I and II, occurred most frequently.

Clinical, microbiological and hormonal examination of women with chronic cervicitis revealed lesions in the upper section of the reproductive tract in a high proportion of those examined, hormonal disturbances being registered in 96.7% of women. Dysbiotic manifestations (suppression of lacto- and bifidoflora and the excessive growth of opportunistic microorganisms) in the uterus cervix and vagina observed in patients with chronic cervititis were not associated with the etiology of the inflammatory process. The degree of dysmicrobiocenosis in the lower section of the genital tract in women with chronic cervicitis depends on the character of hormonal disturbances. The most significant inhibition of the resident flora was observed when ovarian dysfunction occurred and less significant--in cases of hyperprolactinemia and changes in the level of hypophysial hormones. Hormonal disturbances led to contamination of vagina and cervical canal with opportunistic microorganisms that was inversely proportional to the presence of lactic acid bacteria and bifidobacteria in these organs. Complex therapy of women with chronic cervicitis with the use of preparations for the correction of hormonal disturbances made it possible to restore the normal microflora of the genital tract and to improve the results of treatment.

Clinico-immunological examination of 99 pregnant women with diagnosed vaginosis and 132 pregnant women without genital infection as a control group, was carried out. The immunological factors of cervical and vaginal secretions in pregnant women before and after local treatment were studied. The conclusion was made that the established changes in the cell-mediated and humoral factors of the immune resistance of the reproductive system could probably play some pathogenetic role in the development of vaginosis and its relapses in pregnant women. As found in this study, more pronounced changes in the local factors of immune protection (the signs of the functional irritation of neutrophils in combination with the prevalence of sIgA and lysozyme simultaneously with a decrease in the level of IgM and IgG in cervical slime) developed in patients with subsequent relapses of vaginosis, these changes remaining after local treatment. The defects of cell-mediated and humoral factors of cervical and humoral secretions, together with some clinical parameters, were shown to be prognostically unfavorable with respect to the relapses of vaginosis in pregnant women.

66 women of reproductive age with different course of the inflammatory process in the upper section of the reproductive tract (endometritis and salpingo-oophoritis) were examined. The cell composition, viability and functional activity of the phagocytizing cells of cervical and endometrial secretions, as well as peritoneal exudate, were studied. The study revealed that these characteristics of the phagocytizing cells of the reproductive tract in women with the inflammatory process differed from similar characteristics in healthy women. Different changes in the functional activity of neutrophils and macrophages in the biological fluids under study in different course of the inflammatory process were detected.

In 101 healthy pregnant women and 132 pregnant women with genital infection (colpitis, endocervicitis, cervicitis) the characteristics of the anti-infectious protection of cervical mucus were studied. In pregnant women with inflammatory diseases of the vagina and cervix uteri disturbances in the local immunity of the sex system were detected. The study showed that in genital infections local immunodeficiency depended to a greater extent on the localization and spread of the process rather than on the etiological factor of the disease.

Healthy girls and women of the reproductive age, as well as women immediately before and after menopause, were examined. Neutrophils and immunoglobulins of cervical and vaginal secretions were studied and, as a result, age-dependent differences in the activity of the anti-infectious protection of the reproductive tract of women were found.

Pregnant women with genital infection were locally treated with lysozyme and immunoglobulins. The use of these preparations was found to normalize the characteristics of local immunity, to influence the composition of the genital microflora and to lead to the disappearance of the clinical symptoms of the disease and to a decrease in the frequency of relapses of the inflammatory process.

The secretory products of the neutrophils were shown to exert an activating effect on the erythropoiesis in case of an activating neutrophil supernatant. The latter seems to modify the reactivity of the central macrophages in animals with suppressed endogenous erythropoietin.

The effects of activated neutrophilic secretory products on marrow erythroblastic islet erythropoiesis and peripheral blood parameters were studied after experimental blood loss. The supernatant of activated neutrophils was demonstrated to potentiate erythrodieresis in early post-loss period. At the same time the supernatant had a high stimulant effect on erythroblastic islet erythropoiesis. The erythroblastic islets showed increases in new formation and reconstitution, acceleration of amplification wave, which results in peripheral blood reticulocytosis. Thus, neutrophilic secretory products stimulates the synthesis and secretion of central macrophage products, which promotes accelerated maturation of erythroblasts in the erythroblastic islet crown.