Author: "H, Zhai" / Topic: female - Searchworks@Jio Institute Digital Library Search Results

28 results on '"H, Zhai"'

1. [Intraoperative ultrasound assisted circumferential decompression for multilevel ossification of the posterior longitudinal ligament in thoracic vertebrae]

Author: S H, Zhai, P P, Hu, and X G, Liu
Subjects: Adult, Male, Blood Loss, Surgical, Middle Aged, Ossification of Posterior Longitudinal Ligament, Decompression, Surgical, Thoracic Vertebrae, Longitudinal Ligaments, 论著, Spinal Fusion, Treatment Outcome, Osteogenesis, Humans, Female, Retrospective Studies
Abstract: OBJECTIVE: To analyze the effect of short-segment circumferential decompression and the nerve function improvement in 30 cases of multilevel thoracic OPLL assisted by intraoperative ultrasound. METHODS: A total of 30 patients with multilevel thoracic OPLL from January 2016 to January 2021 were enrolled, all of whom were located by intraoperative ultrasound and underwent circumferential decompression. There were 14 males and 16 females, with an average age of (49.3±11.4) years. The initial symptoms were mainly numbness and weakness of lower limbs (83.3%), and the mean duration of symptoms was (33.9±42.9) months (1-168 months). Neurological function was assessed by the Modified Japanese Orthopedic Association (mJOA) score (0-11) preoperative and at the last follow-up, in which the rate of neurological improvement was calculated by the Harabayashi method. The patients were divided into excellent improved group and poor improved group according to the improvement of neurological function. The age, body mass index (BMI), duration of symptoms, operation time, blood loss, mJOA score, surgical level, and cerebrospinal fluid leakage of the two groups were collected and analyzed for statistical differences. The factors influencing the improvement of neurological function were analyzed by univariate and multivariate Logisitic regression analysis. RESULTS: The mean operation time was 137.4±33.8 (56-190) min, and the mean blood loss was (653.7±534.2) mL (200－3 000 mL). The preoperative mJOA score was 6.0±2.1 (2－9), and the last follow-up mJOA score was 7.6±1.9 (4－11), which was significantly improved in all the patients (P < 0.001). The average improvement rate of neurological function was 38.1%±24.4% (14.3%－100%), including 75%－100% in 4 cases, 50%－74% in 3 cases, 25%－49% improved in 14 cases, and 0%－24% in 9 cases. There was significant difference in intraoperative blood loss between the excellent improved group and the poor improved group (P=0.047). Intraoperative blood loss was also an independent risk factor in regression analysis of neurological improvement. CONCLUSION: Thoracic circumferential decompression assisted with intraoperative ultrasound can significantly improve the neurological function of patients with multilevel OPLL and achieve good efficacy. The improvement rate of nerve function can be improved effectively by controlling intraoperative blood loss.
Published: 2022

2. [Efficacy of basiliximab in the treatment of 87 cases of steroid-refractory or steroid-dependent acute graft-versus-host disease]

Author: Z X, He, R L, Zhang, W H, Zhai, Q L, Ma, A M, Pang, D L, Yang, Y, He, J L, Wei, X, Chen, E L, Jiang, S Z, Feng, and M Z, Han
Subjects: Adult, Male, Basiliximab, Acute Disease, Hematopoietic Stem Cell Transplantation, Graft vs Host Disease, Humans, Female, Steroids, Middle Aged, Child, Retrospective Studies
Published: 2022

3. [Allo-HSCT for acute myeloid leukemia with myelodysplastic-related changes: a clinical analysis]

Author: H X, Zhang, A M, Pang, X, Chen, R L, Zhang, W H, Zhai, Q L, Ma, D L, Yang, J L, Wei, Y, He, S Z, Feng, M Z, Han, and E L, Jiang
Subjects: Adult, Male, Acute myeloid leukemia with myelodysplastic related changes, 基因突变, Hematopoietic Stem Cell Transplantation, 造血干细胞移植, Graft vs Host Disease, Middle Aged, Prognosis, Genetic mutations, 论著, Leukemia, Myeloid, Acute, Myelodysplastic Syndromes, Humans, Female, Retrospective Studies, 急性髓系白血病伴骨髓增生异常相关改变
Abstract: 目的评价异基因造血干细胞移植（allo-HSCT）治疗急性髓系白血病伴骨髓增生异常相关改变（AML-MRC）的疗效及预后因素，分析AML-MRC患者基因突变谱系并探讨影响移植预后的分子生物学特征。方法对2006年至2020年于中国医学科学院血液病医院接受allo-HSCT的75例AML-MRC患者进行回顾性分析。将75例患者分为：H组［既往有骨髓增生异常综合征（MDS）或MDS/骨髓增殖性肿瘤（MPN）病史］、C组（新诊断的AML-MRC伴MDS相关细胞遗传学异常）和M组（新诊断的AML-MRC伴多系发育异常），分析三组患者临床特征差异以及对移植预后的影响。对43例患者行骨髓靶向二代测序（137个基因）。结果 ①75例AML-MRC患者，男41例，女34例，中位年龄41（18～56）岁，中位随访时间为35（95％ CI 30～49）个月，中位总生存（OS）时间为78个月（95％CI 23个月～未达到）。移植后3年OS率为57.1％（95％CI 45.6％～71.4％），无事件生存（EFS）率为52.0％（95％CI 40.8％～66.1％），累积复发率（CIR）为26.8％（95％CI 16.6％～30.0％），移植相关死亡率（TRM）为22.7％（95％ CI 13.2％～33.8％）。多因素分析显示，移植前处于非第1次完全缓解（CR1）状态是移植后OS和EFS的独立危险因素。影响OS的独立危险因素还包括−5/5q−染色体核型异常、移植后未发生慢性移植物抗宿主病（慢性GVHD）。②75例患者中H组59例（78.7％），其中20例转化为白血病（转白）前曾接受去甲基化药物治疗；C组9例（12.0％），M组7例（9.3％）。M、H、C组移植后3年OS率分别为71.4％（95％CI 44.7％～100.0％）、55.0％（95％CI 41.8％～72.5％）、55.6％（95％ CI 31.0％～99.7％）（P＝0.700），EFS率分别为71.4％（95％CI 44.7％～100.0％）、46.5％（95％CI 34.0％～63.8％）、55.6％（95％CI 31.0％～99.7％）（P＝0.600）；原发性AML-MRC与继发性AML-MRC相比，移植后3年OS、EFS率差异无统计学意义［61.9％（95％CI 41.9％～91.4％）对55.0％（95％ CI 41.8％～72.5％），P＝0.600；61.9％（95％CI 41.9％～91.4％）对46.5％（95％CI 34.0％～63.8％），P＝0.400］。转白前接受去甲基化治疗（20例）与未接受去甲基化治疗（39例）患者比较，转白时间差异无统计学意义［195（16～937）d对162（9～3167）d，P＝0.804］，且两组患者OS和EFS差异无统计学意义（P＝0.400，P＝0.700）。③对43例（57.3％）患者骨髓样本行二代基因测序，共发现73个突变类型，检出率最高的是U2AF1（11例，25.6％），其他检出率>10％的突变包括RUNX1（10例，23.3％）、NRAS（10例，23.3％）、ASXL1（6例，14.0％）、PTPN11（5例，11.6％）、TET2（5例，11.6％）。单因素分析显示U2AF1［P＝0.875，HR＝1.110（95％ CI 0.295～4.195）］、RUNX1［P＝0.685，HR＝0.728（95％ CI 0.157～3.375）］、NRAS［P＝0.919，HR＝0.923（95％CI 0.196～4.334）］突变对移植后OS没有影响。结论 −5/5q−染色体异常、未发生慢性GVHD、移植前非CR1状态是影响AML-MRC患者移植后OS的独立危险因素；MHC亚组分类不是影响移植预后的因素；去甲基化药物治疗可能无助于延缓MDS患者转白以及延长移植后OS期。
Published: 2021

4. [Clinical and prognosis analysis of children with kidney retransplantation]

Author: M H, Yu, L, Miao, Y H, Zhai, J, Chen, X Y, Fang, Q F, Miao, J L, Liu, J J, Liu, X S, Tang, Z Q, Zhang, L, Zhang, L, Zeng, H, Xu, and Q, Shen
Subjects: Male, Reoperation, Adolescent, Graft Survival, Humans, Female, Child, Kidney, Prognosis, Kidney Transplantation, Tissue Donors, Retrospective Studies
Published: 2021

5. [The correlation study on human respiratory syncytial virus daily incidence and meteorological parameters in the main urban area of Chongqing from 2009 to 2019]

Author: X Y, Liu, X, Long, H, Zhai, S Y, Chen, Y, Deng, X H, Xie, N, Zang, J, Xie, Z X, Luo, J, Luo, Q B, Li, Z, Fu, L, Ren, and E M, Liu
Subjects: Male, China, Incidence, Respiratory Syncytial Virus, Human, Humans, Infant, Female, Respiratory Syncytial Virus Infections, Child, Correlation of Data, Respiratory Tract Infections
Published: 2021

6. [Syngeneic hematopoietic stem cell transplantation for aplastic anemia]

Author: Y, Zhang, D L, Yang, R L, Zhang, W H, Zhai, A M, Pang, Y, He, E L, Jiang, S Z, Feng, and M Z, Han
Subjects: Adult, Male, Transplantation Conditioning, 同基因, Hematopoietic Stem Cell Transplantation, 造血干细胞移植, Anemia, Aplastic, Graft vs Host Disease, Syngeneic, 论著, Young Adult, Humans, Female, 再生障碍性贫血, 植入失败, Graft failure, Aplastic anemia, Retrospective Studies
Abstract: 目的评价同基因造血干细胞移植（syn-HSCT）治疗再生障碍性贫血（AA）的疗效。方法对11例接受syn-HSCT的AA患者进行回顾性分析。结果全部11例AA患者中男4例，女7例，中位年龄为22（7～44）岁。全部11例患者移植后均获得造血重建，中位粒细胞植入时间为10（8～23）d，中位血小板植入时间为11（8～28）d。8例患者获得长期稳定植入，3例患者植入失败，其中2例行二次移植（1例获得到长期稳定植入，另1例造血重建后再次发生植入失败）。中位随访时间为53（5～135）个月，全部11例患者均存活，其中9例患者外周血象长期正常。结论 syn-HSCT治疗AA可获得良好的长期生存，植入失败仍是有待解决的主要问题。
Published: 2021

7. Dietary choline supplementation regulated lipid profiles of egg yolk, blood, and liver and improved hepatic redox status in laying hens

Author: Q. H. Zhai, J. M. Tong, and X. F. Dong
Subjects: medicine.medical_specialty, Very low-density lipoprotein, food.ingredient, Apolipoprotein B, Choline, Random Allocation, 03 medical and health sciences, chemistry.chemical_compound, food, Phosphatidylcholine, Internal medicine, Yolk, medicine, Animals, 030304 developmental biology, chemistry.chemical_classification, Glutathione Peroxidase, 0303 health sciences, biology, Triglyceride, Superoxide Dismutase, Cholesterol, Glutathione peroxidase, 0402 animal and dairy science, 04 agricultural and veterinary sciences, General Medicine, Lipid Metabolism, Animal Feed, Egg Yolk, Lipids, 040201 dairy & animal science, Diet, Endocrinology, Liver, chemistry, Phosphatidylcholines, biology.protein, Female, lipids (amino acids, peptides, and proteins), Animal Science and Zoology, Chickens, Oxidation-Reduction
Abstract: Five hundred and forty 19-wk-old HyLine Brown laying hens were randomly distributed to 6 dietary treatments and fed 1of 6 corn-soybean meal-based diets added into choline with 0, 425, 850, 1,700, 3,400, and 6,800 mg/kg to investigate effects of dietary choline supplementation on lipid profiles of egg yolk, serum and liver, and hepatic redox status of laying hens. Yolk weight and total lipid, triglyceride, cholesterol and phosphatidylcholine, serum triglyceride, cholesterol, apolipoprotein B 100 (apoB 100), and very low density lipoprotein (VLDL), and liver relative weight, total lipid, triglyceride and apoB 100 as well as hepatic total superoxide dismutase and glutathione peroxidase (GSH-Px) activities, total antioxidant capacity (T-AOC), and malondialdehyde (MDA) in laying hens at weeks 58 and 68 of age were determined. The differences (P0.001) were caused by choline treatments in yolk phosphatidylcholine (at 850 mg/kg or more choline), serum VLDL, and liver triglyceride (at 1,700 and 3,400 mg/kg choline) of birds, at weeks 58 and 68 of age, and yolk total lipids were elevated (P0.05) by supplemental choline at 3,400 mg/kg whereas liver total lipids were reduced (P0.05) by 1,700 and 3,400 mg/kg choline addition. Hens fed diets supplemented choline had higher (P = 0.005) liver GSH-Px activity (with 3,400 mg/kg choline) and greater (P = 0.014) T-AOC (with 1,700 mg/kg choline) than those fed diets with 0 and 425 mg/kg choline addition. Choline affected serum VLDL, liver total lipid, triglyceride and apoB 100 at weeks 58 and 68 of age and hepatic GSH-Px activity, T-AOC and MDA at week 68 of age quadratically (P0.05), whereas it influenced total lipid and phosphatidylcholine of egg yolk linearly (P0.05) and quadratically (P0.05). In conclusion, dietary choline supplementation elevated yolk total lipid and phosphatidylcholine and serum VLDL, reduced liver total lipid and triglyceride, and enhanced hepatic GSH-Px activity and T-AOC in laying hens.
Published: 2019

8. [Clinical analysis of 2 820 cases of drug-induced liver injury]

Author: F J, Song, Q H, Zhai, Q J, He, S, Lyu, B, Zhu, T J, Xu, H, Tian, S J, Xin, and S L, You
Subjects: Adult, Male, Cholestasis, Liver, Hepatocytes, Humans, Female, Chemical and Drug Induced Liver Injury, Middle Aged, Anti-Bacterial Agents, Retrospective Studies
Published: 2020

9. Hsa_circ_0011946 promotes the migration and invasion of hepatocellular carcinoma by inducing EMT process

Author: L, Ren, H, Zhai, X-L, Wang, J-Z, Li, and Y-H, Xia
Subjects: Adult, Male, Carcinoma, Hepatocellular, Epithelial-Mesenchymal Transition, Cell Movement, Liver Neoplasms, Humans, Female, Neoplasm Invasiveness, Hep G2 Cells, RNA, Circular, Middle Aged
Abstract: Recently, the vital role of circular RNAs is discovered in many diseases including tumor progression and metastasis. Hepatocellular carcinoma (HCC) is one of the most ordinary malignant tumors. The purpose of our study is to detect the potential function of hsa_circ_0011946 in HCC to offer new biomarkers and targets.The level of hsa_circ_0011946 in HCC tissues and cell lines was monitored by Real Time Quantitative Polymerase Chain Reaction (RT-qPCR). Pearson's Chi-square test was used to determine the association between hsa_circ_0011946 expression and several clinicopathological factors. Then, hsa_circ_0011946 was knocked down in HCC cells to uncover its function in metastasis of HCC. Cell migrated and invaded ability was measured through transwell assay, Matrigel assay and wound healing assay. Western blot assay was performed to analyze the effect of hsa_circ_0011946 on the epithelial-to-mesenchymal transition (EMT) process.In this research, the expression level of hsa_circ_0011946 was significantly increased in HCC tissues compared to that in adjacent samples. The expression of hsa_circ_0011946 was also increased in HCC cell lines. The hsa_circ_0011946 expression was associated with lymphatic metastasis in HCC patients. Knockdown of hsa_circ_0011946 led to the inhibition of cell migration and invasion in HCC. In addition, results of further experiments revealed that the EMT-related proteins were regulated via the knockdown of hsa_circ_0011946 in HCC.The hsa_circ_0011946 could enhance cell migration and invasion of HCC by inducing the EMT process.
Published: 2020

10. Risk profiles and one-year outcomes of patients with newly diagnosed atrial fibrillation in India: Insights from the GARFIELD-AF Registry

Author: Jitendra PS. Sawhney, Veerappa A. Kothiwale, Vikas Bisne, Rajashekhar Durgaprasad, Praveen Jadhav, Manoj Chopda, Velam Vanajakshamma, Ramdhan Meena, Govindan Vijayaraghavan, Kamaldeep Chawla, Jagan Allu, Karen S. Pieper, A. John Camm, Ajay K. Kakkar, Jean-Pierre Bassand, David A. Fitzmaurice, Samuel Z. Goldhaber, Shinya Goto, Sylvia Haas, Werner Hacke, Lorenzo G. Mantovani, Frank Misselwitz, Alexander G.G. Turpie, Martin van Eickels, Freek W.A. Verheugt, Gloria Kayani, Keith A.A. Fox, Bernard J. Gersh, Hector Lucas Luciardi, Harry Gibbs, Marianne Brodmann, Frank Cools, Antonio Carlos Pereira Barretto, Stuart J. Connolly, Alex Spyropoulos, John Eikelboom, Ramon Corbalan, Dayi Hu, Petr Jansky, Jørn Dalsgaard Nielsen, Hany Ragy, Pekka Raatikainen, Jean-Yves Le Heuzey, Harald Darius, Matyas Keltai, Sanjay Kakkar, Jitendra Pal Singh Sawhney, Giancarlo Agnelli, Giuseppe Ambrosio, Yukihiro Koretsune, Carlos Jerjes Sánchez Díaz, Hugo Ten Cate, Dan Atar, Janina Stepinska, Elizaveta Panchenko, Toon Wei Lim, Barry Jacobson, Seil Oh, Xavier Viñolas, Marten Rosenqvist, Jan Steffel, Pantep Angchaisuksiri, Ali Oto, Alex Parkhomenko, Wael Al Mahmeed, David Fitzmaurice, D.Y. Hu, K.N. Chen, Y.S. Zhao, H.Q. Zhang, J.Z. Chen, S.P. Cao, D.W. Wang, Y.J. Yang, W.H. Li, Y.H. Yin, G.Z. Tao, P. Yang, Y.M. Chen, S.H. He, Ying Wang, Yong Wang, G.S. Fu, X. Li, T.G. Wu, X.S. Cheng, X.W. Yan, R.P. Zhao, M.S. Chen, L.G. Xiong, P. Chen, Y. Jiao, Y. Guo, L. Xue, F.Z. Wang, H. Li, Z.M. Yang, C.L. Bai, J. Chen, J.Y. Chen, X. Chen, S. Feng, Q.H. Fu, X.J. Gao, W.N. Guo, R.H. He, X.A. He, X.S. Hu, X.F. Huang, B. Li, J. Li, L. Li, Y.H. Li, T.T. Liu, W.L. Liu, Y.Y. Liu, Z.C. Lu, X.L. Luo, T.Y. Ma, J.Q. Peng, X. Sheng, X.J. Shi, Y.H. Sun, G. Tian, K. Wang, L. Wang, R.N. Wu, Q. Xie, R.Y. Xu, J.S. Yang, L.L. Yang, Q. Yang, Y. Ye, H.Y. Yu, J.H. Yu, T. Yu, H. Zhai, Q. Zhan, G.S. Zhang, Q. Zhang, R. Zhang, Y. Zhang, W.Y. Zheng, B. Zhou, Z.H. Zhou, X.Y. Zhu, S. Kakkar, J.P.S. Sawhney, P. Jadhav, R. Durgaprasad, A.G. Ravi Shankar, R.K. Rajput, K. Bhargava, R. Sarma, A. Srinivas, D. Roy, U.M. Nagamalesh, M. Chopda, R. Kishore, G. Kulkarni, P. Chandwani, R.A. Pothiwala, M. Padinhare Purayil, S. Shah, K. Chawla, V.A. Kothiwale, B. Raghuraman, G. Vijayaraghavan, V.M. Vijan, G. Bantwal, V. Bisne, A. Khan, J.B. Gupta, S. Kumar, D. Jain, S. Abraham, D. Adak, A. Barai, H. Begum, P. Bhattacharjee, M. Dargude, D. Davies, B. Deshpande, P. Dhakrao, V. Dhyani, S. Duhan, M. Earath, A. Ganatra, S. Giradkar, V. Jain, R. Karthikeyan, L. Kasala, S. Kaur, S. Krishnappa, A. Lawande, B. Lokesh, N. Madarkar, R. Meena, P. More, D. Naik, K. Prashanth, M. Rao, N.M. Rao, N. Sadhu, D. Shah, M. Sharma, P. Shiva, S. Singhal, S. Suresh, V. Vanajakshamma, S.G. Panse, Y. Koretsune, S. Kanamori, K. Yamamoto, K. Kumagai, Y. Katsuda, K. Sadamatsu, F. Toyota, Y. Mizuno, I. Misumi, H. Noguchi, S. Ando, T. Suetsugu, M. Minamoto, Hiroshi Oda, K. Shiraishi, S. Adachi, K. Chiba, H. Norita, M. Tsuruta, T. Koyanagi, H. Ando, T. Higashi, K. Okada, S. Azakami, S. Komaki, K. Kumeda, T. Murayama, J. Matsumura, Y. Oba, R. Sonoda, K. Goto, K. Minoda, Y. Haraguchi, H. Suefuji, H. Miyagi, H. Kato, Tadashi Nakamura, Tsugihiro Nakamura, H. Nandate, R. Zaitsu, Yoshihisa Fujiura, A. Yoshimura, H. Numata, J. Ogawa, H. Tatematsu, Y. Kamogawa, K. Murakami, Y. Wakasa, M. Yamasawa, H. Maekawa, S. Abe, H. Kihara, S. Tsunoda, Katsumi Saito, Kazuyuki Saito, T. Fudo, K. Obunai, H. Tachibana, I. Oba, T. Kuwahata, S. Higa, M. Gushiken, T. Eto, H. Yoshida, D. Ikeda, Yoshitake Fujiura, M. Ishizawa, M. Nakatsuka, K. Murata, C. Ogurusu, M. Shimoyama, M. Akutsu, I. Takamura, F. Hoshino, N. Yokota, T. Iwao, K. Tsuchida, M. Takeuchi, Y. Hatori, Y. Kitami, Yoichi Nakamura, R. Oyama, M. Ageta, Hiroyuki Oda, Y. Go, K. Mishima, T. Unoki, S. Morii, Yuhei Shiga, H. Sumi, T. Nagatomo, K. Sanno, K. Fujisawa, Y. Atsuchi, T. Nagoshi, T. Seto, T. Tabuchi, M. Kameko, K. Nii, K. Oshiro, H. Takezawa, S. Nagano, N. Miyamoto, M. Iwaki, Yuichiro Nakamura, M. Fujii, M. Okawa, Masahiko Abe, Masatake Abe, Mitsunori Abe, T. Saito, T. Mito, K. Nagao, J. Minami, T. Mita, I. Sakuma, T. Taguchi, S. Marusaki, H. Doi, M. Tanaka, T. Fujito, M. Matsuta, T. Kusumoto, S. Kakinoki, K. Ashida, N. Yoshizawa, J. Agata, O. Arasaki, M. Manita, M. Ikemura, S. Fukuoka, H. Murakami, S. Matsukawa, Y. Hata, T. Taniguchi, T. Ko, H. Kubo, M. Imamaki, M. Akiyama, M. Inagaki, H. Odakura, T. Ueda, Y. Katsube, A. Nakata, H. Watanabe, M. Techigawara, M. Igarashi, K. Taga, T. Kimura, S. Tomimoto, M. Shibuya, M. Nakano, K. Ito, T. Seo, S. Hiramitsu, H. Hosokawa, M. Hoshiai, M. Hibino, K. Miyagawa, Hajime Horie, N. Sugishita, Yukio Shiga, A. Soma, K. Neya, Tetsuro Yoshida, Tomoki Yoshida, M. Mizuguchi, M. Ishiguro, T. Minagawa, M. Wada, H. Mukawa, F. Okuda, S. Nagasaka, Y. Abe, Sen Adachi, Susumu Adachi, T. Adachi, K. Akahane, T. Amano, K. Aoki, T. Aoyama, H. Arai, S. Arima, T. Arino, H. Asano, T. Asano, J. Azuma, T. Baba, T. Betsuyaku, H. Chibana, H. Date, J. Doiuchi, Y. Emura, M. Endo, Y. Fujii, R. Fujiki, A. Fujisawa, Y. Fujisawa, T. Fukuda, T. Fukui, N. Furukawa, T. Furukawa, W. Furumoto, T. Goto, M. Hamaoka, N. Hanazono, K. Hasegawa, T. Hatsuno, Y. Hayashi, K. Higuchi, K. Hirasawa, H. Hirayama, M. Hirose, S. Hirota, M. Honda, Hideki Horie, T. Ido, O. Iiji, H. Ikeda, K. Ikeda, K. Ikeoka, M. Imaizumi, H. Inaba, T. Inoue, F. Iseki, A. Ishihara, N. Ishioka, N. Ito, T. Iwase, H. Kakuda, J. Kamata, H. Kanai, H. Kanda, M. Kaneko, H. Kano, T. Kasai, T. Kato, Y. Kato, Y. Kawada, K. Kawai, K. Kawakami, S. Kawakami, T. Kawamoto, S. Kawano, J. Kim, T. Kira, H. Kitazawa, H. Kitazumi, T. Kito, T. Kobayashi, T. Koeda, J. Kojima, H. Komatsu, I. Komatsu, Y. Koshibu, T. Kotani, T. Kozuka, Y. Kumai, T. Kumazaki, I. Maeda, K. Maeda, Y. Maruyama, S. Matsui, K. Matsushita, Y. Matsuura, K. Mineoi, H. Mitsuhashi, N. Miura, S. Miyaguchi, S. Miyajima, H. Miyamoto, A. Miyashita, S. Miyata, I. Mizuguchi, A. Mizuno, T. Mori, O. Moriai, K. Morishita, O. Murai, Sho Nagai, Shunichi Nagai, E. Nagata, H. Nagata, A. Nakagomi, S. Nakahara, M. Nakamura, R. Nakamura, N. Nakanishi, T. Nakayama, R. Nakazato, T. Nanke, J. Nariyama, Y. Niijima, H. Niinuma, Y. Nishida, Y. Nishihata, K. Nishino, H. Nishioka, K. Nishizawa, I. Niwa, K. Nomura, S. Nomura, M. Nozoe, T. Ogawa, N. Ohara, M. Okada, K. Okamoto, H. Okita, M. Okuyama, H. Ono, T. Ono, Y. Onuki Pearce, S. Oriso, A. Ota, E. Otaki, Y. Saito, H. Sakai, N. Sakamoto, Y. Sakamoto, Y. Samejima, Y. Sasagawa, H. Sasaguri, A. Sasaki, T. Sasaki, Kazuki Sato, Kiyoharu Sato, M. Sawano, S. Seki, Y. Sekine, Y. Seta, K. Sezaki, N. 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A., Andersson, A., Malmqvist, L., Martinsson, B., Bernsten, F., Engdahl, J., Thulin, J., Hot-Bjelac, A., Stalby, P., Aaröe, H., Ahbeck, E., Ahlmark, H., Al-Khalili, F., Bonkowski, G., Dzeletovic, S., Ekstrand, A. -B., Eriksson, G. -B., Floren, K., Grässjö, C., Hahn, S., Jaensson, P., Jansson, B., Jansson, J. -H., Kangert, R. -M., Koch, A., Kusiak, D., Lettenström, A., Lindberg, A., Lindholm, C. -J., Mannermyr, A., Mansson, K., Millborg, M., Nilsson, C., Ohlin, A. -M., Olofsson, A., Osberg, A., Pedersen, A., Risbecker, K., Rosenberg, K., Samuelsson, J., Shayesteh, M., Skoglund, K., Stjernberg, M., Thorsen, C., Steffel, J., Beer, J. H., Debrunner, J., Amstutz, D., Bruegger, J., Elise, G., Grau, A., Guinand, A., Henriette, I., Saga, E., Winnik, S., Parkhomenko, A., Rudyk, I., Tseluyko, V., Karpenko, O., Zhurba, S., Kraiz, I., Kupnovytska, I., Serediuk, N., Mostovoy, Y., Ushakov, O., Koval, O., Kovalskyi, I., Svyshchenko, Y., Sychov, O., Stanislavchuk, M., Kraydashenko, O., Yagensky, A., Tykhonova, S., Fushtey, I., Belegai, R., Berko, G., Burdeuna, L., Chabanna, O., Daniuk, I., Ivanov, A., Kamenska, E., Kaplan, P., Khyzhnyak, O., Kizim, S., Matova, O., Medentseva, O., Mochonyi, V., Mospan, M., Nemtsova, V., Ovdiienko, T., Palamarchuk, O., Pavelko, M., Petrovskyy, R., Plevak, D., Proshak, O., Pyvovar, S., Rasputina, L., Romanenko, O., Romanova, O., Sapatyi, A., Shumakov, O., Stets, R., Todoriuk, L., Varenov, V., Fitzmaurice, D., Chauhan, N., Goodwin, D., Saunders, P., Evans, R., Leese, J., Jhittay, P. S., Ross, A., Kainth, M. S., Pickavance, G., Mcdonnell, J., Williams, A., Gooding, T., Wagner, H., Suryani, S., Singal, A., Sircar, S., Bilas, R., Hutchinson, P., Wakeman, A., Stokes, M., Paul, N., Aziz, M., Ramesh, C., Wilson, P., Franklin, S., Fairhead, S., Thompson, J., St Joseph, V., Taylor, G., Tragen, D., Seamark, D., Paul, C., Richardson, M., Jefferies, A., Sharp, H., Jones, H., Giles, C., Page, M., Oginni, O., Aldegather, J., Wetherwell, S., Lumb, W., Evans, P., Scouller, F., Macey, N., Stipp, Y., West, R., Thurston, S., Wadeson, P., Matthews, J., Pandya, P., Gallagher, A., Railton, T., Sinha, B., Russell, D., Davies, J. A., Ainsworth, P., Jones, C. P., Weeks, P., Eden, J., Kernick, D., Murdoch, W., Lumley, L., Patel, R. P., Wong, S. W., Saigol, M., Ladha, K., Douglas, K., Cumberlidge, D. F., Bradshaw, C., Van Zon, G., Jones, K. P., Thomas, M. J., Watson, E., Sarai, B., Ahmad, N., Willcock, W., Cairns, J., Sathananthan, S., de Kare-Silver, N., Gilliland, A., Strieder, E., Howitt, A., Vishwanathan, B., Bird, N., Gray, D., Clark, M., Bisatt, J., Litchfield, J., Fisher, E., Fooks, T., Kelsall, A. R., Alborough, E., Wakeling, J., Parfitt, M., Milne, K., Rogers, S., Priyadharshan, R., Oliver, J. L., Davies, E., Abushal, S., Jacobs, M., Hutton, C., Walls, N. I., Thompson, R., Chigbo, C., Zaidi, S. M. A., Howard, M., Butter, K. C., Barrow, S., Little, H., Haq, I. U., Gibbons, L., Glencross, S., Mcleod, A. J., Poland, K., Mulholland, C., Warke, A., Conn, P., Burns, G., Smith, R. N., Lowe, S., Kamath, R., Dau, H. S., Webster, J., Hodgins, I., Vercoe, S., Roome, P. C., Pinnock, H., Patel, J. R. A., Ali, A., Hart, N., Davies, R., Stuart, E., Neden, C. A., Danielsen, M., Heath, R., Sharma, P., Galloway, S., Hawkins, C., Oliver, R., Aylward, M., Mannion, S., Braddick, M., Edwards, D., Rothwell, A. C., Sabir, A., Choudhary, F., Khalaque, S., Wilson, A., Peters, S., Coulson, W., Roberts, N., Heer, A., Coates, S., Ward, B., Jackson, D., Walton, S., Shepherd, D., Sterry, M., Wong, T., Boon, M., Bunney, R., Haria-Shah, R., Baron, R. T., Davies, S., Schatzberger, T., Hargreaves, N., Stephenson, T., Choi, H., Batson, R., Lucraft, L., Myhill, T., Estifano, S., Geatch, D., Wilkinson, J., Veale, R., Forshaw, K., Davies, T., Zaman, K., Vinson, P., Liley, C., Bandrapalli, M., Mcginty, P., Wastling, R., Mceleny, P., Beattie, A., Cooke, P., Wong, M., Gunasegaram, J., Pugsley, M., Ahmad, S., A'Court, C., Ayers, J., Bennett, J., Cartwright, S., Dobson, S., Dooldeniya, C., Flynn, A., Fox, R., Goram, J., Halpin, A., Hay, A., Jacobs, P., Jeffers, L., Lomax, L., Munro, I., Muvva, R., Nadaph, M., Powell, K., Randfield, S., Redpath, D., Reed, R., Rickenbach, M., Rogers, G., Saunders, P. B., Seamark, C., Shewring, J., Simmons, P., Simper, H., Stoddart, H., Sword, A., Thomas, N., Thomson, A., Gibbs, H., Blenkhorn, A., Singh, B., Van Gaal, W., Abhayaratna, W., Lehman, R., Roberts-Thomson, P., Kilian, J., Coulshed, D., Catanchin, A., Colquhoun, D., Kiat, H., Eccleston, D., French, J., Zimmett, L., Ayres, B., Phan, T., Blombery, P., Crimmins, D., O'Donnell, D., Choi, A., Astridge, P., Arstall, M., Jepson, N., Binnekamp, M., Lee, A., Rogers, J., Starmer, G., Carroll, P., Faunt, J., Aggarwala, A., Barry, L., Batta, C., Beveridge, R., Black, A., Bonner, M., Boys, J., Buckley, E., Campo, M., Carlton, L., Connelly, A., Conway, B., Cresp, D., Dimitri, H., Dixon, S., Dolman, M., Duroux, M., Eskandari, M., Eslick, R., Ferreira-Jardim, A., Fetahovic, T., Fitzpatrick, D., Geraghty, R., Gibbs, J., Grabek, T., Modi, M. H., Hayes, K., Hegde, M. P., Hesketh, L., Hoffmann, B., Jacobson, B., Johnson, K., Juergens, C., Kassam, I., Lawlor, V., Lehman, M., Lehman, S., Leung, D., Mackay, S., Mackenzie, M., Mccarthy, C., Mcintosh, C., Mckeon, L., Morrison, H., Mussap, C., Myers, J. -D., Nagalingam, V., Oldfield, G., O'May, V., Palmer, J., Parsons, L., Patching, K., Patching, T., Paul, V., Plotz, M., Preston, S., Rashad, H., Ratcliffe, M., Raynes, S., Rose, J., Sanders, L., Seremetkoska, M., Setio, H., Shone, S., Shrestha, P., Singh, C., Singleton, C., Stoyanov, N., Sutcliffe, S., Swaraj, K., Tarrant, J., Thompson, S., Tsay, I. M., Vorster, M., Waldman, A., Wallis, L., Wilford, E., Wong, K., Connolly, S. J., Spyropoulos, A., Eikelboom, J., Luton, R., Gupta, M., Pandey, A. S., Cheung, S., Leader, R., Beaudry, P., Ayala-Paredes, F., Berlingieri, J., Heath, J., Poirier, G., Du Preez, M., Nadeau, R., Dresser, G., Dhillon, R., Hruczkowski, T., Schweitzer, B., Coutu, B., Angaran, P., Macdonald, P., Vizel, S., Fikry, S., Parkash, R., Lavoie, A., Cha, J., Ramjattan, B., Bonet, J., Ahmad, K., Aro, L., Aves, T., Beaudry, K., Bergeron, C., Bigcanoe, J., Bignell, N., Breakwell, L., Burke, E., Carroll, L., Clarke, B., Cleveland, T., Daheb, S., Dehghani, P., Denis, I., Djaidani, Z., Dorian, P., Douglass, S., Dunnigan, J., Ewert, A., Farquhar, D., Fearon, A., Ferleyko, L., Fournier, D., Fox, B., Grenier, M. -C., Gulliver, W., Haveman, K., Hines, C., Hines, K., Jackson, A. M., Jean, C., Jethoo, G., Kahlon, R., Kelly, S., Kim, R., Korley, V., Kornder, J., Kwan, L., Largy, J., Lewis, C., Lewis, S., Mangat, I., Moor, R., Navratil, J., Neas, I., Otis, J., Otis, R., Pandey, M., Petrie, F., Pinter, A., Raines, M., Roberts, P., Robinson, M., Sas, G., Schulman, S., Snell, L., Spearson, S., Stevenson, J., Trahey, T., Wong, S., Wright, D., Ragy, H., Abd El-Aziz, A., Abou Seif, S. K., El Din, M. G., El Etriby, S., Elbahry, A., El-Etreby, A., Elkhadem, M., Katta, A., Khairy, T., Mowafy, A., Nawar, M., Ohanissian, A., Reda, A., Reda, M., Salem, H., Sami, N., Samir, S., Setiha, M., Sobhy, M., Soliman, A., Taha, N., Tawfik, M., Zaatout, E., Kettles, D., Bayat, J., Siebert, H., Horak, A., Kelfkens, Y., Garda, R., Pillay, T., Guerra, M., van Zyl, L., Theron, H., Murray, A., Louw, R., Greyling, D., Mntla, P., Ueckermann, V., Loghdey, R., Ismail, S., Ahmed, F., Engelbrecht, J., Ramdass, A., Maharajh, S., Oosthuysen, W., Angel, G., Bester, C., Booysen, M., Boshoff, C., Cannon, C., Cassimjee, S., Chami, C., Conway, G., Davids, A., de Meyer, L., Du Plessis, G., Ellis, T., Henley, L., Karsten, M., Loyd, E., Marks, J., Mavhusa, L., Mostert, M., Page, A., Rikhotso, L., Salie, M., Sasto, J., Shaik, F., Skein, A., Smith, L., Tarr, G., Tau, T., van Zyl, F., Al Mahmeed, W., Yousef, G., Agrawal, A., Nathani, M., Ibrahim, M., Esheiba, E. M., Singh, R., Naguib, A., Abu-Mahfouz, M., Al Omairi, M., Al Naeemi, A., Maruthanayagam, R., Bazargani, N., Wassef, A., Gupta, R., Khan, M., Subbaraman, B., Abdul, A., Al Mulla, A., El Bardisy, S., Haridas, P., Jadhav, S., Magdaluyo, K., Makdad, M., Maqsood, I., Mohamed, R., Sharma, N., Sharma, R., Thanzeel, M., Goldhaber, S. Z., Canosa, R., Rama, P., Blumberg, E., Garcia, J., Mullen, P., Wilson, V., Quick, A., Ferrick, K., Kutayli, W. M., Cox, M., Franco, M., Falkowski, S., Mendelson, R., Williams, M., Miller, S., Beach, S., Alfieri, A., Gutowski, T., Haque, I., Reddy, R., Ahmed, W., Delafontaine, P., Diercks, D., Theodoro, D., Remmel, K., Alberts, M., Ison, R., Noveck, H., Duffy, P., Pitta, S., Nishijima, D., Treasure, C., Asafu-Adjaye, N., Ball, K., Bartlett, M., Bentley, M., Bowers, S., Brown, A., Browne, A., Cameron-Watts, J., Canova, M., Cassidy, D., Cervellione, K., Congal, S., Depauw, J., Dickerson, A., Eley, M., Evans, L., Felpel, S., Ferdinand, K., Fielder, D., Gentry, P., Haideri, A., Hakimi, F., Harbour, T., Hartranft, E., Hawkins, B., Headlee, M., Henson, L., Herrick, C., Hicks, T., Jasinski, S., Jones, A., Jones, L., Jones, P., Karl, S., Keeling, M., Kerr, J., Knowles, P., Langdon, J., Lay, M., Lee, J. A., Lincoln, T., Malone, E., Merliss, A., Merritt, D., Minardo, J., Mooso, B., Orosco, C., Palumbo, V., Parker, M., Parrott, T., Paserchia, S., Pearl, G., Peterson, J., Pickelsimer, N., Purcell, T., Raynor, J., Raziano, S., Richard, C., Richardson, T., Robertson, C., Sage, A., Sanghera, T., Shaw, P., Shoemaker, J., Smith, K., Stephanie, B., Thatcher, A., Theobald, H., Thompson, N., Treasure, L., Tripti, T., Verdi, C., and Worthy, V.
Subjects: Registrie, Male, Time Factors, Anticoagulant therapy, Arrhythmia, Atrial fibrillation, GARFIELD-AF, 030204 cardiovascular system & hematology, Electrocardiography, 0302 clinical medicine, Risk Factors, Anticoagulant therapy, Arrhythmia, Atrial fibrillation, GARFIELD-AF, Aged, Atrial Fibrillation, Female, Follow-Up Studies, Humans, Incidence, India, Prevalence, Prognosis, Prospective Studies, Survival Rate, Thromboembolism, Registries, Risk Assessment, Medicine, 030212 general & internal medicine, Prospective cohort study, Incidence (epidemiology), cardiovascular system, medicine.symptom, Cardiology and Cardiovascular Medicine, Risk assessment, Human, medicine.medical_specialty, Time Factor, RD1-811, Prognosi, Follow-Up Studie, 03 medical and health sciences, Internal medicine, Diseases of the circulatory (Cardiovascular) system, cardiovascular diseases, Risk factor, Survival rate, Fibrillation, business.industry, Risk Factor, ta3121, medicine.disease, Clinical trial, Prospective Studie, RC666-701, Surgery, Clinical and Preventive Cardiology, business
Abstract: Background: The Global Anticoagulant Registry in the FIELD–Atrial Fibrillation (GARFIELD-AF) is an ongoing prospective noninterventional registry, which is providing important information on the baseline characteristics, treatment patterns, and 1-year outcomes in patients with newly diagnosed non-valvular atrial fibrillation (NVAF). This report describes data from Indian patients recruited in this registry. Methods and results: A total of 52,014 patients with newly diagnosed AF were enrolled globally; of these, 1388 patients were recruited from 26 sites within India (2012–2016). In India, the mean age was 65.8 years at diagnosis of NVAF. Hypertension was the most prevalent risk factor for AF, present in 68.5% of patients from India and in 76.3% of patients globally (P
Published: 2018

11. RNA sequencing identified specific circulating miRNA biomarkers for early detection of diabetes retinopathy

Author: Wen H. Yang, Li Tian, Ri H. Zhai, Jing Y. Ding, Xin Z. Yang, Yi L. Li, Wei T. Wu, Zi C. Liu, Yi X. Wang, Kai P. Gao, Ze B. Zhang, and Zhen Liang
Subjects: Adult, Male, 0301 basic medicine, Circulating mirnas, Diabetes retinopathy, medicine.medical_specialty, endocrine system diseases, Physiology, Endocrinology, Diabetes and Metabolism, Early detection, Real-Time Polymerase Chain Reaction, Bioinformatics, Sincalide, 03 medical and health sciences, Physiology (medical), Internal medicine, Diabetes mellitus, microRNA, medicine, Humans, In patient, Aged, Cell Proliferation, Diabetic Retinopathy, Sequence Analysis, RNA, business.industry, Endothelial Cells, RNA, Diabetic retinopathy, Middle Aged, medicine.disease, MicroRNAs, 030104 developmental biology, Endocrinology, ROC Curve, Case-Control Studies, Female, Endothelium, Vascular, business, Biomarkers
Abstract: Diabetic retinopathy (DR) is the leading cause of blindness in patients with diabetes. However, biomarkers for early detection of DR are still lacking. MicroRNAs (miRNAs) regulate multiple biological functions and are often deregulated in DR. We aimed to investigate whether circulating miRNAs can be used as biomarkers of early-stage DR. We used RNA-seq and qRT-PCR to identify differential serum miRNAs in patients with type 2 diabetes mellitus with DR (T2DM-DR), T2DM without DR (T2DM-no-DR), and healthy controls. We validated differential circulating miRNAs in two phases using qRT-PCR assays. RNA-seq analysis identified 7 differential circulating miRNAs between T2DM-DR and T2DM-no-DR and 47 differential miRNAs between T2DM-DR and healthy subjects. Two-stage analysis verified that a profile of five serum miRNAs (hsa-let-7a-5p, hsa-miR-novel-chr5_15976, hsa-miR-28-3p, has-miR-151a-5p, has-miR-148a-3p) was significantly associated with T2DM-DR. Receiver-operator-characteristic analyses showed that a panel of three miRNAs (hsa-let-7a-5p, hsa-miR-novel-chr5_15976, and hsa-miR-28-3p) presented 0.92 sensitivity and 0.94 specificity for distinguishing T2DM-DR from T2DM-no-DR, and 0.93 sensitivity and 0.86 specificity for differentiating early-stage T2DM-DR (NPDR) from late-stage DR (PDR). Lentivirus-mediated overexpression of hsa-let-7a-5p in human retinal microvascular endothelial cells (HRMECs) significantly promoted proliferation rates of HRMECs. In conclusion, the three-miRNA signature from serum may serve as a noninvasive diagnostic biomarker for DR. Furthermore, we showed that DR-associated miRNAs may be involved in the pathogenesis of DR, at least in part, through modifying proliferation of HRMECs.
Published: 2018

12. [Value of intravoxel incoherent motion diffusion weighted imaging in evaluating the microstructure changes in white matter hyperintensities]

Author: D, Yang, Y, Chen, Y F, Dong, X L, Liu, F L, Fu, L H, Zhai, X B, Xu, S Y, Hu, and J J, Zhang
Subjects: Aged, 80 and over, Male, Perfusion, Motion, Diffusion Magnetic Resonance Imaging, Humans, Female, Middle Aged, White Matter, Aged
Published: 2019

13. [Prognostic significance of early phase donor chimerism after allogeneic peripheral blood stem cell transplantation]

Author: W H, Zhai, Q Z, Liu, Y Y, Shi, G, Li, J L, Sun, X, Chen, J F, Yao, X H, Su, Q L, Ma, A M, Pang, Y, He, D L, Yang, R L, Zhang, Y, Huang, J L, Wei, S Z, Feng, M Z, Han, and E L, Jiang
Subjects: Adult, Male, Peripheral Blood Stem Cell Transplantation, Adolescent, Prediction factors, Hematopoietic Stem Cell Transplantation, Graft vs Host Disease, Middle Aged, 预测因子, Prognosis, Chimerism, 论著, Young Adult, 造血干细胞移植，异基因, 嵌合状态, Child, Preschool, Humans, Female, Child, Retrospective Studies
Abstract: 目的评价清髓性异基因外周血干细胞移植（allo-PBSCT）后早期完全供者嵌合状态（FDC）对患者预后的预测意义。方法回顾性分析2016年2月至2017年7月行清髓性allo-PBSCT治疗的72例血液病患者临床资料。男44例，女28例，中位年龄36.5（4~59）岁。其中同胞全合供者35例、亲缘单倍型相合供者27例、无关供者10例。采用聚合酶链反应扩增短串联重复序列（PCR-STR）检测移植后1、2、3个月受者骨髓的供者细胞嵌合（DC）率。结果全部72例患者中位随访462（47~805）d，其中55例存活，2年总体生存（OS）率为（68.9±7.7）%；45例无病存活，2年无病生存（DFS）率为（59.5±6.3）%；16例复发，中位复发时间为移植后157（32~374）d，2年复发率（RR）为（24.1±5.3）%。40例（55.6%）患者发生急性移植物抗宿主病（aGVHD），中位发生时间为移植后35.5（13~90）d；23例（31.9%）发生慢性GVHD（cGVHD），中位发生时间为移植后169（94~475）d。单因素分析显示患者年龄、性别、供受者血型、供受者性别、是否发生aGVHD和cGVHD均不影响OS、DFS率及RR。移植后2个月达到FDC组患者OS、DFS率均高于未达FDC组[OS：（85.2±6.9）%对（66.1±7.7）%，P=0.051；DFS：（76.7±7.7）%对（48.9±8.1）%，P=0.021]，RR低于未达FDC组[（16.6±6.8）%对（30.4±7.8）%，P=0.187]。结论清髓性allo-PBSCT后早期患者是否达到FDC对移植预后具有重要预测价值，移植后2个月达FDC患者OS及DFS率明显高于未达FDC患者。
Published: 2018

14. [Coenzyme Q(10) treatment for one child with COQ6 gene mutation induced nephrotic syndrome and literature review]

Author: Q, Cao, G M, Li, H, Xu, Q, Shen, L, Sun, X Y, Fang, H M, Liu, W, Guo, Y H, Zhai, and B B, Wu
Subjects: Male, Proteinuria, Nephrotic Syndrome, Ubiquinone, Homozygote, Mutation, Humans, Female, Genes, Recessive, Vitamins, Child, Kidney
Published: 2017

15. Long-term effects of choline on productive performance and egg quality of brown-egg laying hens

Author: Q. H. Zhai, Y. E. Bao, Y. M. Guo, J. M. Tong, and X. F. Dong
Subjects: Meal, food.ingredient, Eggs, Pigments, Biological, General Medicine, Biology, Animal Feed, Egg laying, Drug Administration Schedule, Choline, Diet, chemistry.chemical_compound, food, chemistry, Yolk, Animals, Animal Nutritional Physiological Phenomena, Female, Animal Science and Zoology, Food science, Chickens
Abstract: A total of five hundred forty 19-wk-old HyLine Brown hens were used to study the long-term effects of increasing choline with 0 (control), 425, 850, 1,700, 3,400, and 6,800 mg/kg of corn-soybean meal-based diets on productive performance and egg quality. Phase 1 was from 19 to 58 wk, and phase 2 was from 59 to 68 wk. During the whole experimental period, dietary choline had no significant effects on feed intake, egg weight, and egg mass (P0.05). During phase 1, egg production decreased linearly (P0.05) and feed conversion ratio (FCR) tended to increase linearly (P = 0.057) with increasing choline level in the diet. Moreover, BW decreased both linearly (P0.01) and quadratically (P0.05) as choline increased from 0 to 6,800 mg/kg. No significant treatment effects were found for shell thickness and shell strength of eggs (P0.05). However, albumen height and Haugh units increased linearly (P0.01 and P0.05, respectively) as choline increased during phase 2. Compared with the control group, diets supplemented with 425 or 850 mg of choline/kg significantly (P0.01) improved yolk color during phase 1. This study indicates that a dietary choline level of no more than 700 mg/kg is sufficient to maintain egg production. The effect of choline on egg quality was minimal when hens were fed a corn-soybean meal-based diet from 19 to 68 wk of age.
Published: 2013

16. New Buccinator Myomucosal Island Flap: Anatomic Study and Clinical Application

Author: Z, Zhao, S, Li, Y, Yan, Y, Li, M, Yang, L, Mu, W, Huang, Y, Liu, H, Zhai, J, Jin, and X, Ma
Subjects: Adult, Male, medicine.medical_specialty, media_common.quotation_subject, Facial Muscles, Poison control, Shame, Scars, Anger, Suicide prevention, Surgical Flaps, Cadaver, medicine, Humans, Stigmata, Meaning (existential), Child, media_common, integumentary system, business.industry, General surgery, Mouth Mucosa, Middle Aged, medicine.disease, Surgery, Facial Nerve, Cheek, Child, Preschool, Female, Munchausen syndrome, medicine.symptom, business
Abstract: The authors studied the vascular anatomy of the buccinator muscle by dissecting fresh cadavers. The anatomy of the buccal branches of the facial artery consistently confirmed the existence of a posterior buccal branch, a few inferior buccal branches, and anterior buccal branches to the posterior, inferior, and anterior portions of the buccinator. The buccal artery and posterior buccal branch anastomose to each other and ramify over the muscle. Several veins originate from the lateral aspect of the muscle, converge into the buccal venous plexus, and drain into the facial vein (from two to four tributaries) or into the pterygoid plexus and the internal maxillary vein (from the buccal vein). These vessels and nerves enter the posterior half of the buccinator posterolaterally. The facial artery and vein are located at variable distances from each other around the oral commissure and the nasal base. Two patterns of buccinator musculomucosal island flaps supplied by these buccal arterial branches are proposed in this article. The buccal musculomucosal neurovascular island flap (posteriorly based), supplied by the buccal artery, its posterior buccal branch, and the long buccal nerve, can be passed through a tunnel under the pterygomandibular ligament for closure of mucosal defects in the palate, pharyngeal sites, the alveolus, and the floor of the mouth. The buccal musculomucosal reversed-flow arterial island flap (superiorly based), supplied by the distal portion of the facial artery through the anterior buccal branches, can be used to close mucosal defects in the anterior hard palate, alveolus, maxillary antrum, nasal floor and septum, lip, and orbit. The authors have used the flaps in 12 patients. There has been no flap necrosis, and results have been satisfactory, both aesthetically and functionally.
Published: 1999

17. Influence of CYP2C9 and CYP2C19 genetic polymorphisms on pharmacokinetics and pharmacodynamics of gliclazide in healthy Chinese Han volunteers

Author: H, Shao, X M, Ren, N F, Liu, G M, Chen, W L, Li, Z H, Zhai, and D W, Wang
Subjects: Adult, Blood Glucose, Male, China, Polymorphism, Genetic, Cytochrome P-450 CYP2C19, Young Adult, Asian People, Tandem Mass Spectrometry, Area Under Curve, Gliclazide, Humans, Hypoglycemic Agents, Insulin, Female, Aryl Hydrocarbon Hydroxylases, Chromatography, Liquid, Cytochrome P-450 CYP2C9, Half-Life
Abstract: CYP2C9 is the major contributor to gliclazide metabolic clearance in vitro, while the pharmacokinetics of gliclazide modified release are affected mainly by CYP2C19 genetic polymorphisms in vivo. This study aims to investigate the influence of CYP2C9 and CYP2C19 genetic polymorphisms on the pharmacokinetics and pharmacodynamics of gliclazide in healthy Chinese Han volunteers.Eighteen healthy Han subjects with various combinations of CYP2C9 and CYP2C19 genotypes received 80 mg gliclazide. Plasma gliclazide concentrations were measured by a liquid chromatography-tandem mass spectrometry method for 84 h and plasma glucose and insulin levels were measured up to 15 h post-dose.There was no difference in either pharmacokinetic and or pharmacodynamic parameters of gliclazide when group A (CYP2C9*1/*1, CYP2C19 extensive metabolizers) was compared with group B (CYP2C9*1/*3, CYP2C19 *1/*1). When group C (CYP2C9*1/*1 and CYP2C19 poor metabolizers) was compared with group A, the AUC(0-∞) and C(max) in group C were significantly higher [83.94 ± 40.41 vs. 16.39 ± 5.10 μg·h/mL (P = 0.000) and 1.50 ± 0.85 vs. 0.45 ± 0.18 μg/mL (P = 0.000)], and the oral clearance was significantly lower [1.17 ± 0.63 vs. 5.38 ± 1.86 L/h (P = 0.000)]. The half-life of gliclazide was also significantly prolonged in group C subjects when compared with that of group A (33.47 ± 12.39 vs. 19.34 ± 10.45 h), but the difference was not significant (P = 0.052). The increase in serum glucose level at 11 h after dosing (ΔC(glu11)) in group C was significantly higher than that of group A (-1.08 ± 0.42 vs. 0.22 ± 1.01 mmol/L, P = 0.022). The corresponding insulin levels showed no difference between the two groups.CYP2C9*3 was not associated with any change in the disposition of gliclazide. CYP2C19 polymorphisms appear to exert the dominant influence on the pharmacokinetics of gliclazide in healthy Chinese Han subjects, and may also affect the observed pharmacodynamics of the drug as a result.
Published: 2010

18. [Effects of short veno-venous hemofiltration on severe acute pancreatitis]

Author: E, Mao, Y, Tang, T, Han, H, Zhai, Z, Yuan, H, Yin, and S, Zhang
Subjects: Male, Treatment Outcome, Pancreatitis, Acute Necrotizing, Interleukins, Humans, Female, Hemofiltration
Abstract: To estimate the effects of therapy of short veno-venous hemofiltration (SVVH, 4hr) on severe acute pancreatitis (SAP).Twenty patients were divided randomly into hemofiltration group (HF, 10 pts) and non-hemofiltration group (NHF, 10 pts). The local, systemic manifestations were compared between two groups; and the concentration of serum pro-inflammatory cytokines (TNFalpha, IL-1beta, IL-6, IL-8, sIL-2R) and anti-inflammatory cytokines (IL-2, IL-10) were determined at different observation points.In the HF and NHF group, the duration for disappearance of abdominal pain and tenderness, and amelioration for abdominal distension was (8.5 +/- 6.3) d vs. (90.0 +/- 49.0) d and (16.9 +/- 5.2) d vs. (63.4 +/- 36.0) d. CT scores at the 14th day and APACHE II scores at the 10th day was (5.6 +/- 1.3) vs. (7.7 +/- 0.8) and (4.5 +/- 1.5) vs. (7.4 +/- 2.1). The average hospital stay and cost of therapy were (33.0 +/- 20.7) d vs. (56.4 +/- 16.0) d and (53,200 +/- 16,000) vs. (89,100 +/- 25,000) RMB (P0.05). The concentration of 5 serum pro-inflammatory cytokines at each observation points after hemofiltration was decreased significantly (P0.05) in the HF group. But the concentration of the two serum anti-inflammatory cytokines was increased significantly (P0.05) as compared with that of the NHF group.Through SVVH, the imbalance of pro-inflammatory and anti-inflammatory cytokines has been corrected at early stage; thus, the severity of the disease is ameliorated significantly and the efficacy of therapy is increased.
Published: 2002

19. [Effect of stannius corpuscles homogenates of carp on plasma calcium and phosphate in mice]

Author: W G, Li, X H, Zhai, and Y R, Luo
Subjects: Male, Mice, Carps, Tissue Extracts, Animals, Calcium, Female, Phosphorus, Hormones, Glycoproteins
Published: 2001

20. [Anti-tumor effect of cytotoxic T lymphocytes induced by soluble antigen of ovarian carcinoma and anti-CD3 monoclonal antibody on ovarian carcinoma]

Author: Q, Zhang, Y, Wan, and H, Zhai
Subjects: Cytotoxicity, Immunologic, Ovarian Neoplasms, Mice, Inbred BALB C, CD3 Complex, Antibodies, Monoclonal, Mice, Nude, Immunotherapy, Adoptive, Mice, Antigens, Neoplasm, Leukocytes, Mononuclear, Tumor Cells, Cultured, Animals, Humans, Female, Killer Cells, Lymphokine-Activated, Neoplasm Transplantation, T-Lymphocytes, Cytotoxic
Abstract: To evaluate the therapeutic effect of the new method--adoptive cell immunotherapy on ovarian carcinoma.Tumor soluble antigen (TSA) was extracted from cells of ovarian carcinoma (COC1 and COC2n). To generate cytotoxic T lymphocytes (CTL), peripheral blood mononuclear cells (PBMC) collected from healthy persons were induced by TSA and anti-CD3 monoclonal antibody (CD3-McAb). The antitumor effect of CTL on COC1 cells in vitro was compared with LAK and CD3-AK, whereas the growth inhibitory effect of CTL on COC2n transplantation tumors in nude mice was compared with CD3-AK.The cytotoxicity of CTL, CD3-AK and LAK was 79.4%, 52.1% and 51.7% respectively (P0.01). Mean volume of tumor in nude mice on the 9th day after ovarian carcinoma cells transplanted was 44.4 +/- 24.2 mm3 in CTL group, 118.8 +/- 40.0 mm3 in CD3-AK group and 443.0 +/- 158.7 mm3 in control (no treatment) (P0.01), whereas mean duration of survival was 28.5 days, 25.5 days and 17 days respectively (P0.01).The results indicated that CTL exhibited higher cytotoxic effect in vitro and greater inhibitory effect on tumor growth resulting in longer survival in vivo. It suggested that the adoptive cell immunotherapy might provide a new idea for treatment of ovarian carcinoma.
Published: 2000

21. Strontium nitrate suppresses chemically-induced sensory irritation in humans

Author: H, Zhai, W, Hannon, G S, Hahn, A, Pelosi, R A, Harper, and H I, Maibach
Subjects: Adult, Male, Nitrates, Time Factors, Pruritus, Glycolates, Keratolytic Agents, Double-Blind Method, Strontium, Sensation Disorders, Irritants, Dermatitis, Irritant, Humans, Female, Dermatologic Agents, Paresthesia, Skin
Abstract: Skin care products are complex formulations that may cause sensory irritation symptoms, characterized by stinging, burning, and itching. Substances capable of counteracting sensory irritation are of great practical interest. Strontium salts have been demonstrated to inhibit sensory irritation and inflammation when applied topically. In this double-blind study, we evaluated the efficacy of strontium nitrate in reducing chemically-induced skin sensory irritation in 8 subjects. In a random order, 20% strontium nitrate in 70% glycolic acid (pH=0.6) (mixture) was applied to the volar aspect of the forearm and a positive control (70% glycolic acid, pH=0.6) to the contralateral forearm. The irritation sensation was evaluated each min for the first 20 min after topical application using a scale from 0-4. The duration of the irritation sensation in min was also recorded. Strontium nitrate mixed with glycolic acid, in comparison with glycolic acid alone, markedly (p0.01) shortened the duration of the irritation sensation from 24.4+/-4.1 (mean+/-SEM) min to 8.9+/-3.7 (mean+/-SEM) min, and significantly (p0.05) reduced the mean magnitude of the irritation sensation at all time points (overall). The study demonstrated that strontium nitrate potently suppresses the sensation of chemically-induced irritation.
Published: 2000

22. Human barrier recovery after acute acetone perturbation: an irritant dermatitis model

Author: H, Zhai, Y H, Leow, and H I, Maibach
Subjects: Acetone, Adult, Male, Time Factors, Solvents, Dermatitis, Irritant, Humans, Female, Dermatologic Agents, Middle Aged, Water Loss, Insensible, Skin
Abstract: The efficacy of a topical agent in barrier recovery was evaluated after acetone-induced acute water loss barrier disruption in vivo in humans. The upper back of several volunteers was rubbed with acetone-soaked cotton balls until elevated rates of transepidermal water loss (TEWL) occurred (20 g/m2h, or greater). The topical agent was then applied to the acetone-treated skin sites once daily for 5 days. Resolution evaluation used TEWL measurements and the data were expressed as the percentage recovery in water barrier function. In comparison with placebo control the topical agent significantly enhanced barrier recovery, especially within the first 72 h (P0.05). This model offers a simple method of examining chemicals accelerating (or inhibiting) repair of this form of acute skin damage in man.
Published: 1998

23. [Nuclear reconstitution around purified E. coli DNA in cell-free extracts of Xenopus laevis eggs]

Author: Z F, Jiang, J, Qu, and Z H, Zhai
Subjects: Cell Nucleus, DNA Replication, DNA, Bacterial, Xenopus laevis, Cell-Free System, Tissue Extracts, Escherichia coli, Oocytes, Animals, Female
Abstract: In recent years we have succeeded in inducing various exogenous DNA to assembly typical nuclei in cell-free Xenopus egg extracts. However, it is not clear if prokaryotic chromosomal DNA can also form the nucleus in this eukaryotic system. In our experiment, we used the purified E. coli. chromosomal DNA to reconstitute the nucleus in the nuclear reconstitution extracts and found that typical nuclei can also be assembled around added DNA. We observed the process of nuclear reconstitution by using light microscopy and electronic microscopy. The scanning results of chromatin-like materials and assembled nuclei by microspectrophotometer showed that E. coli, chromosal DNA underwent obvious change from condensation to decondensation during nuclear reconstitution. Incorporation of alpha-32P-dCTP into DNA suggested that the reconstituted nuclei were capable of DNA replication.
Published: 1997

24. [The roles of two kinds of membrane vesicles in the formation of annulate lamellae and nuclear envelopes in a cell-free system from Xenopus egg extracts]

Author: B, Zhang and Z H, Zhai
Subjects: Xenopus laevis, Cell-Free System, Nuclear Envelope, Animals, Female, Membrane Fusion, Chromatin, Ovum
Abstract: The eggs of Xenopus laevis were activated to complete their second meiosis and enter interphase by calcium ionophore A 23187. Then they were centrifuged at 10,000 g to get activated Xenopus egg extracts. Chromatin structure could form in such an extracts after exogenous Lambda DNA was added, and nuclear envelopes (NEs) could assemble around the chromatin. Furthermore, annulate lamellae (AL), a NE-like membrane structure, could form in chromatin-free regions simultaneously. After large amounts of observation and analysis at ultrastructural level, we propose that both AL and NEs assemble in the extracts by the fusion of membrane vesicles. There were two kinds of membrane vesicles in Xenopus egg extracts, small rough vesicles with diameters of 200 nm and large smooth vesicles. They had many differences in morphology, size and membrane structure. However, they both participate in the formation of AL and NEs, 200 nm membrane vesicles first fused each other to become parallel double membrane cisternae, nuclear pore complexes assembled in the double membranes in the mean time. The fusion of large smooth vesicles with the double membrane cirternae led to the growth of AL and the maturity of NEs. During the formation of NEs, large smooth vesicles were easily observed to link two neighboring double membrane fragments by fusing with them, so that integrated nuclear envelope could form. 200 nm small rough vesicles could attach to the surface of chromatin to start the assembly of nuclear envelopes, while others could fuse each other at chromatin-free regions and these fusion resulted in the formation of annulate lamellae.
Published: 1995

25. Electron microscopic analysis of the relationship between nuclear matrix stability and cell differentiation

Author: L, Yang, E C, Chew, S B, Cheng-Chew, H F, Yam, E P, Loong, and Z H, Zhai
Subjects: Uterine Cervical Neoplasms, Cell Differentiation, Epithelial Cells, Cervix Uteri, Epithelium, Cell Line, Rats, Microscopy, Electron, Reference Values, Carcinoma, Squamous Cell, Tumor Cells, Cultured, Animals, Humans, Female, Nuclear Matrix, Cell Nucleolus
Abstract: Two cell lines, the less differentiated CC2/CUHK2 and the more differentiated CC3/CUHKE3, were used to study the difference in nuclear matrix stability against DNase 1 digestion. The nuclear matrix was almost totally extracted when the CC3/CUHK3 cells were digested with 100 micrograms/ml DNase 1, while that of the CC2/CUHK2 cells was still present even when 200 micrograms/ml DNase 1 was used. It is suggested that more differentiated cells have a less stable nuclear matrix while the less differentiated ones have a more stable nuclear matrix. The same phenomenon was also observed in normal human and rat cervical epithelia. The nuclear matrix of the poorly differentiated basal cells was more stable than that of the more differentiated superficial cells. This cell differentiation stage dependent stability of the nuclear matrix is probably related to the nuclear activity and gene expression.
Published: 1994

26. Fine structural observation of a nucleolar-nuclear matrix-lamina-intermediate filament system in transformed cells

Author: L, Yang, E C, Chew, S B, Chew-Cheng, R J, Jiao, H F, Yam, and Z H, Zhai
Subjects: Macropodidae, Carcinoma, Squamous Cell, Intermediate Filaments, Animals, Humans, Uterine Cervical Neoplasms, Female, Nuclear Matrix, Cell Nucleolus, Cell Line, Transformed
Abstract: The nuclear matrix is a nonchromatin structure of the nucleus normally concealed by a much larger mass of chromatin. Several methods have been developed to remove chromatin from nucleus while preserving the underlying matrix architecture to some degree. The present study showed that after extraction of PtK2 cells and cervical carcinoma cells (CC3) with Triton X-100, ammonium sulfate and DNase, the nucleolar-nuclear matrix-intermediate filament (Nu-Nm-L-IF) network remained. The nucleolus was oval in shape and appeared as a fibrillar mass with an accentric dense fibrillar centre. This nucleolar skeleton was in direct connection with the nuclear matrix which in turn is connected with cytoplasmic intermediate filaments by lamins. It is concluded from these observations that the Nu-NM-L-IF system forms a continuous system which plays an important role in the maintenance of the nucleolar, nuclear and cytoplasmic integrity and cellular function.
Published: 1994

27. [Nuclear reconstitution around purified HeLa chromosomes in a cell-free system from Xenopus eggs]

Author: C M, Zhang, J, Qu, and Z H, Zhai
Subjects: Cell Nucleus, Xenopus laevis, Cell-Free System, Tissue Extracts, Animals, Humans, Female, Chromosomes, Metaphase, HeLa Cells, Ovum
Abstract: Activated Xenopus egg extracts are capable of reconstituting nucleus around the added DNA or chromatin. To understand better the nuclear reconstitution process and the structure of the assembled nucleus, we have developed a cell-free system using activated Xenopus egg extracts and metaphase chromosomes of HeLa cells. We demonstrate that the extracts are efficient promoting the decondensation of the metaphase chromosomes and the nuclear reconstitution around the chromosomes, and that the nuclear reconstitution is a precise process. The reassembled nucleus possesses a typical nuclear envelope, pore complexes, nuclear lamina, nuclear matrix, chromatin, etc., as shown by electron microscopy, embedment and embedment-free electron microscopy, and immunofluorescence method. We also found that the annulate lamellae directly take part in nuclear envelope and pore complex reconstitution by fusing with the double membrane, having reassembled on the surface of chromatin/chromosomes.
Published: 1994

28. [Changes in peripheral leukocytic glucocorticoid receptor in patients with ischemic and hemorrhagic stroke]

Author: Y, Zhao, Z M, Liu, and C H, Zhai
Subjects: Adult, Aged, 80 and over, Male, Receptors, Glucocorticoid, Hydrocortisone, Leukocytes, Humans, Female, Intracranial Embolism and Thrombosis, Middle Aged, Prognosis, Aged, Cerebral Hemorrhage
Abstract: The maximal binding capacity (Ro) and dissociation constant (Kd) of glucocorticoid receptor (GCR) in peripheral leukocytes were estimated by radioligand binding method and the plasma cortisol levels measured in 25 stroke patients (hemorrhagic 15, ischemic 10), compared with 12 healthy controls, the plasma cortisol level in stroke patients were significantly higher. The plasma cortisol level of 15 hemorrhagic and 10 ischemic stroke patients and 12 controls determined by RIA were 1.12 +/- 0.21, 0.90 +/- 0.28 and 0.45 +/- 0.09 nmol/L respectively. Ro of leukocytic GCR in hemorrhagic stroke were significantly lower than that of ischemic stroke and controls. No difference was found between ischemic stroke and controls. No difference was found between ischemic stroke and controls. Ro of GCR in 15 hemorrhagic stroke, 10 ischemic stroke patients and 12 controls were 3496 +/- 424, 5678 +/- 1101 and 5940 +/- 763 sites/cell respectively kd of GCR were 10.47 +/- 4.17, 7.01 +/- 3.24 and 5.81 +/- 1.05 nmol/L respectively. Affinity of GCR in 15 hemorrhagic stroke patients was significantly lower. In conclusion, lower number and affinity of leukocytic GCR and higher plasma cortisol level were demonstrated in hemorrhagic stroke patients, but normal number and affinity of GCR and higher plasma cortisol level in ischemic stroke patients.
Published: 1989

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28 results on '"H, Zhai"'

1. [Intraoperative ultrasound assisted circumferential decompression for multilevel ossification of the posterior longitudinal ligament in thoracic vertebrae]

2. [Efficacy of basiliximab in the treatment of 87 cases of steroid-refractory or steroid-dependent acute graft-versus-host disease]

3. [Allo-HSCT for acute myeloid leukemia with myelodysplastic-related changes: a clinical analysis]

4. [Clinical and prognosis analysis of children with kidney retransplantation]

5. [The correlation study on human respiratory syncytial virus daily incidence and meteorological parameters in the main urban area of Chongqing from 2009 to 2019]

6. [Syngeneic hematopoietic stem cell transplantation for aplastic anemia]

7. Dietary choline supplementation regulated lipid profiles of egg yolk, blood, and liver and improved hepatic redox status in laying hens

8. [Clinical analysis of 2 820 cases of drug-induced liver injury]

9. Hsa_circ_0011946 promotes the migration and invasion of hepatocellular carcinoma by inducing EMT process

10. Risk profiles and one-year outcomes of patients with newly diagnosed atrial fibrillation in India: Insights from the GARFIELD-AF Registry

11. RNA sequencing identified specific circulating miRNA biomarkers for early detection of diabetes retinopathy

12. [Value of intravoxel incoherent motion diffusion weighted imaging in evaluating the microstructure changes in white matter hyperintensities]

13. [Prognostic significance of early phase donor chimerism after allogeneic peripheral blood stem cell transplantation]

14. [Coenzyme Q(10) treatment for one child with COQ6 gene mutation induced nephrotic syndrome and literature review]

15. Long-term effects of choline on productive performance and egg quality of brown-egg laying hens

16. New Buccinator Myomucosal Island Flap: Anatomic Study and Clinical Application

17. Influence of CYP2C9 and CYP2C19 genetic polymorphisms on pharmacokinetics and pharmacodynamics of gliclazide in healthy Chinese Han volunteers

18. [Effects of short veno-venous hemofiltration on severe acute pancreatitis]

19. [Effect of stannius corpuscles homogenates of carp on plasma calcium and phosphate in mice]

20. [Anti-tumor effect of cytotoxic T lymphocytes induced by soluble antigen of ovarian carcinoma and anti-CD3 monoclonal antibody on ovarian carcinoma]

21. Strontium nitrate suppresses chemically-induced sensory irritation in humans

22. Human barrier recovery after acute acetone perturbation: an irritant dermatitis model

23. [Nuclear reconstitution around purified E. coli DNA in cell-free extracts of Xenopus laevis eggs]

24. [The roles of two kinds of membrane vesicles in the formation of annulate lamellae and nuclear envelopes in a cell-free system from Xenopus egg extracts]

25. Electron microscopic analysis of the relationship between nuclear matrix stability and cell differentiation

26. Fine structural observation of a nucleolar-nuclear matrix-lamina-intermediate filament system in transformed cells

27. [Nuclear reconstitution around purified HeLa chromosomes in a cell-free system from Xenopus eggs]

28. [Changes in peripheral leukocytic glucocorticoid receptor in patients with ischemic and hemorrhagic stroke]

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28 results on '"H, Zhai"'

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