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Prenatal sonographic depiction of congenital vertical talus (rocker bottom feet), describing a prominent calcaneus and rounded convex appearance of the ventral aspect of the foot, has been reported with fetal Trisomies 18, 13, 9 HOXD10 mutations and recently 2q13 microdeletion. We present a 24 year old in whom mid-trimester sonographic finding of isolated bilateral rocker bottom feet led to diagnosis of 7q11.23 microdeletion-Williams syndrome. This association has not been reported previously. This case emphasizes the critical assessment of detail microarray upon prenatal sonographic notation of abnormal structural fetal features.

Background: In the United States, hyperemesis gravidarum is the most common cause of hospitalization during the first half of pregnancy and is second only to preterm labor for hospitalizations in pregnancy overall. In approximately 0.3-3% of pregnancies, hyperemesis gravidarum is prevalent and this percentage varies on account of different diagnostic criteria and ethnic variation in study populations. Despite extensive research in this field, the mechanism of the disease is largely unknown. Although cases of mortality are rare, hyperemesis gravidarum has been associated with both maternal and fetal morbidity. The current mainstay of treatment relies heavily on supportive measures until improvement of symptoms as part of the natural course of hyperemesis gravidarum, which occurs with progression of gestational age. However, studies have reported that severe, refractory disease manifestations have led to serious adverse outcomes and to termination of pregnancies. Summary: Despite extensive research in the field, the pathogenesis of hyperemesis gravidarum remains unknown. Recent literature points to a genetic predisposition in addition to previously studied factors such as infectious, psychiatric, and hormonal contributions. Maternal morbidity is common and includes psychological effects, financial burden, clinical complications from nutritional deficiencies, gastrointestinal trauma, and in rare cases, neurological damage. The effect of hyperemesis gravidarum on neonatal health is still debated in literature with conflicting results regarding outcomes of birth weight and prematurity. Available therapy options remain largely unchanged in the past several decades and focus on parenteral antiemetic medications, electrolyte repletion, and nutritional support. Most studies of therapeutic options do not consist of randomized control studies and cross-study analysis is difficult due to considerable variation of diagnostic criteria. Key Messages: Hyperemesis gravidarum carries a significant burden on maternal health and US health care. Most published research on pathogenesis is observational and suggests multifactorial associations with hyperemesis gravidarum. Precise, strictly defined criteria for clinical diagnosis are likely to benefit meta-analyses of further research studies regarding pathogenesis as well as therapeutic options.

A cervical pregnancy is an uncommon ectopic pregnancy that accounts for less than 1% of such gestations. This condition is associated with an extremely high risk of massive hemorrhage and previously often required hysterectomy. The current diagnostic modality of this potentially life-threatening condition is transvaginal sonography, supported at times by magnetic resonance imaging. The definitive diagnostic imaging feature of a cervical pregnancy is the location of a gestational sac in the cervix in the presence of a closed internal uterine cervical os. We report the 3-dimensional transvaginal sonographic findings of a cervical pregnancy at 6 weeks' gestation.

Leiomyomas of the genitourinary tract may originate from the renal pelvis, bladder, urethra, or epididymis. 1 Interestingly, leiomyomas of the bladder are more common among women (76% of cases).2 Development is usually endovesical (63%), yet extravesical (30%) and intramural cases are not rare.3 Patients may be asymptomatic or may have obstructive urinary symptoms, irritative symptoms, hematuria, flank pain, or, rarely, dysmenorrhea or dyspareunia. 2-5 Leiomyomas of the bladder have been reported in association with neurofibromatosis type 1, in which leiomyomas occur most often in the gastrointestinal tract (proximal small bowel) and tend to be multiple.6 Rarely, urinary bladder leiomyomas have been diagnosed during pregnancy 7,8 and in a woman with a previous hysterectomy. 9 Diagnostic imaging modalities used include both transabdominal and transvaginal sonography, computed tomography, and magnetic resonance imaging.10-12 Although asymptomatic, nonobstructive, and nonproblematic leiomyomas may be managed expectantly, treatment is surgical by a transurethral approach, laparoscopy, or laparotomy 1-4 We present color Doppler imaging and 3-dimensional sonographic findings of a woman with a urinary bladder leiomyoma.

Background Prior studies suggest associations between fetal exposure to antimicrobial and paraben compounds with adverse reproductive outcomes, mainly in animal models. We have previously reported elevated levels of these compounds for a cohort of mothers and neonates. Objective We examined the relationship between human exposure to parabens and antimicrobial compounds and birth outcomes including birth weight, body length and head size, and gestational age at birth. Methods Maternal third trimester urinary and umbilical cord blood plasma concentrations of methylparaben (MePB), ethylparaben (EtPB), propylparaben (PrPB), butylparaben (BuPB), benzylparaben (BePB), triclosan (2,4,4′-trichloro-2′-hydroxydiphenyl ether or TCS) and triclocarban (1-(4-chlorophenyl)-3-(3,4-dichlorophenyl) urea or TCC), were measured in 185 mothers and 34 paired singleton neonates in New York, 2007–2009. Results In regression models adjusting for confounders, adverse exposure-outcome associations observed included increased odds of PTB (BuPB), decreased gestational age at birth (BuPB and TCC) and birth weight (BuPB), decreased body length (PrPB) and protective effects on PTB (BePB) and LBW (3′-Cl-TCC) ( p Conclusions This study provides the first evidence of associations between antimicrobials and potential adverse birth outcomes in neonates. Findings are consistent with animal data suggesting endocrine-disrupting potential resulting in developmental and reproductive toxicity.

Prenatal sonographic diagnoses of subcutaneous soft tissue masses include hemangiomas, malformations (capillary, lymphatic, venous, arterial, or mixed), teratomas, and, rarely, sarcomas. 1-5 Prenatally depicted malformations may be seen in association with more complex fetal conditions, including Klippel-Trenaunay-Weber (angio-osteohypertrophy) and Proteus syndromes.6"9 We present an unusual case in which extensive multiple soft tissue tumors encompassing the fetal upper chest, back, nuchal area, and bilateral axillae, considered consistent with multiple large lymphatic malformations noted at 23 weeks' gestation, decreased in size throughout the remainder of gestation. At delivery, relatively small subcutaneous masses remained. Tissue histopathologic findings obtained at biopsy and the neonate's clinical course during which the subcutaneous masses underwent a marked spontaneous decrease in size, were consistent with low-flow mixed lymphatic and venous malformations.

The differential diagnosis of highly vascular, intramural lesions of the myometrium Includes arteriovenous malformation, gestational trophoblastic neoplasia, hemangiomata, sarcoma, and interstitial pregnancy. We present an unusual case in which a patient with irregular uterine hemorrhage in the presence of increasing serum β-human chorionic gonadotropin (β-hCG) levels exhibited transvaginal sonographic and color Doppler imaging findings considered consistent with an interstitial pregnancy. After laparoscopy and curettage yielded normal findings but with the continuously rising serum β-hCG levels and the concern for gestational trophoblastic neoplasia, the patient underwent vaginal hysterectomy. Histopathologic findings confirmed choriocarcinoma.

The purpose of this study is to assess whether serum beta-human chorionic gonadotropin (beta-hCG) levels on day 4 following methotrexate (MTX) treatment in patients with ectopic pregnancy predict successful single-dose therapy or the need for subsequent surgical intervention. Retrospective analysis of patients with ectopic pregnancies treated with MTX (50 mg/m (2)) was conducted. Inclusion criteria for MTX management were serum beta-hCG < 15,000 mU/mL, absent fetal cardiac activity, ultrasonographic gestational sac < 3.5 cm, normal liver function tests, hemodynamically stable patient with no evidence of hemoperitoneum, and informed consent. Day 1, 4, and 7 serum beta-hCG levels were obtained. Outcome parameters included successful single-dose MTX management, the requirement for multiple treatments, and whether subsequent surgery was required. Receiver operator characteristic (ROC) curves were used. P < 0.05 was considered significant throughout. Eighty-three patients were studied. Of these, 60 patients were treated successfully with single doses, 16 patients required two doses, and two patients required three doses of MTX, and five underwent surgical management. Mean day 1 serum beta-hCG levels of patients successfully treated with single-dose MTX was 3938.5 (+/- 589.2 [standard deviation]) versus 1767.65 (+/- 1237.8) mU/mL in patients requiring multiple doses of MTX therapy, ( P < 0.0001). ROC curves for serum beta-hCG levels on days 1, 4, and 7 were 0.449, 0.592, and 0.754, respectively, indicating that only day 7 serum beta-hCG levels were associated with successful single-dose MTX therapy. Serum beta-hCG levels on day 4 of MTX in patients with ectopic pregnancy do not predict successful single-dose therapy or the need for surgery.

Germ cell tumors are most commonly located within the gonads. 1 Rare extragondal locations of these tumors include the retroperitoneum, mediastinum, sacrococcygeum, and central nervous system and are considered to result from aberrant migration of germ cells from the yolk sac during fetal development. 1 - 3 We present the transvaginal sonographic diagnosis of a large retroperitoneal pelvic epidermoid cyst (monodermal teratoma).

Physiologic dilatation of the maternal urinary collecting system occurs in more than 80% of gravidas by mid gestation. 1 Caliceal and ureteral dilation are more common on the right, and, similarly, the degree of caliceal dilatation is more pronounced on the right (15 versus 5 mm). 2 Although most women with hydronephrosis during pregnancy are asymptomatic, a few may have clinical signs and symptoms related to the upper urinary system. 3 When left-sided hydronephrosis is greater than the right, particularly if the patient is symptomatic, the likelihood of a pathologic condition, especially obstructive, should be considered. We present an unusual case in which a large left upper abdominal cystic structure depicted sonographically at mid gestation, initially considered consistent with an ovarian cyst, resulted from marked maternal ureteropelvic obstruction and required nephrostomy.

Cervical pregnancy is an uncommon ectopic pregnancy that accounts for approximately

We present an unusual case of a primigravida with a viable cervical pregnancy diagnosed by transvaginal ultrasound and magnetic resonance imaging. Staggered conservative therapeutic measures included systemic high-dose Methotrexate with Folinic acid rescue followed by bilateral embolization of the uterine arteries in response to active cervical bleeding despite declining serum beta-human chorionic gonadotropin levels. Continued active cervical bleeding responded to local tamponade with an inflated Foley catheter balloon positioned within the cervical canal. Conservative treatment was successful, with complete resolution of the cervical pregnancy, resumption of normal menstrual cycles, and a normal transvaginal ultrasonographic appearance of the cervical canal, documented 8 weeks after the initial diagnosis. This case and review of the literature support that various staggered conservative hemostatic measures may be used at various points in which bleeding may occur in the conservative management algorithm of cervical pregnancy.

Objective The goal of this study was to evaluate the performance of ThinPrep, a liquid-based cytology preparation technique, in comparison with conventionally prepared Papanicolaou smears in detecting cervical pathology. Methods Forty-seven English-language articles published between January 1990 and September 2002 were identified through Medline and manual searches. After elimination of 5 nonprimary articles, 10 unpaired studies, 5 descriptive articles with insufficient or no data, and 3 articles with data that could not be recast into a consistent format, there remained 24 usable articles. Seventeen articles contained data comparing ThinPrep with conventional cytology for 35,172 patients; and 10 articles compared cytology with histology or other gold standard diagnoses for 21,752 patients. Three of these articles contained both types of comparisons. The standard cytology classification into negative, atypical, low-grade (LGSIL) and high-grade (HGSIL) squamous intraepithelial lesions, and carcinoma was applied; other categorization schemes were recoded as necessary. Concordance estimates, based on five-way and dichotomous (normal/abnormal) classifications, were obtained from the 17 studies containing paired cytology data. Sensitivity and specificity rates were obtained from the 10 studies with paired cytology and histology data. Results The two methods tend to agree in 89 and 92% of cases based on the five-level and dichotomous classifications, respectively. ThinPrep was reported as normal in 93.5% of cases of normal conventional smears. The remaining 6.5% of ThinPrep slides were classified as follows: atypical, 4.55%; LGSIL, 1.56%; HGSIL, 0.36%; invasive cancer, 0.007%. Sensitivity rates, relative to histology, were 68% (conventional) and 76% (ThinPrep), and specificity rates were 79% (conventional) and 86% (ThinPrep). Conclusion ThinPrep tends to be more sensitive and specific than conventional smears in detecting cervical dysplasia. The increased sensitivity of ThinPrep results in increased cytologic diagnosis of cervical atypia, LGSIL, HGSIL, and invasive cervical carcinoma.

The differential diagnosis of reflective intrauterine membranes during pregnancy includes amniotic bands of fetal origin or membranes of combined maternal-fetal origin. While the former have been associated with fetal transverse reduction defects or the lethal amniotic band syndrome, the latter are usually benign and consist of a preexisting uterine septation or synechia around which the fetal membranes fold or become enveloped. We present an unusual case in which a patient was noted at 19 weeks' gestation to have an intrauterine membrane of undetermined origin, appearing to contain a pulsating vessel. Color Doppler imaging and Doppler flow velocimetry clearly depicted a pulse synchronous with the maternal heart rate, defining the membrane as amniotic membranes surrounding a preexisting uterine synechia and not a true amniotic band. The pregnancy was otherwise uneventful and the patient delivered a healthy neonate at elective repeat cesarean at which time the presence of the uterine synechia was confirmed.

Traditionally, oligohydramnios has been implemented as a sign of potential fetal compromise and associated with an increased incidence of adverse perinatal morbidity and mortality. Decreased amniotic fluid volume is especially of concern when it occurs in conjunction with structural fetal anomalies, fetal growth restriction, postdates pregnancies, and maternal disease. Consequently, following ultrasonographic diagnosis of oligohydramnios at term, delivery is routinely advocated even in otherwise uncomplicated pregnancies with an appropriate-for-gestational-age fetus, irrespective of the presence of reassuring fetal evaluation and the absence of maternal disease. Numerous factors complicate the ultrasonographic diagnosis of oligohydramnios. These include a lack of complete detailed understanding of the physiology of the dynamics of oligohydramnios, the transient condition at times of decreased amniotic fluid volume, generally poor performance of ultrasonography in detecting oligohydramnios, an array of different ultrasound diagnostic criteria, and varying ultrasonographic thresholds. In light of the latter and the lack of prospective randomized data, is unclear that the practice of effecting delivery for isolated oligohydramnios at term is justified. This article presents physiologic dynamics of amniotic fluid, factors that may affect amniotic fluid volume, possible pitfalls in the ultrasonographic assessment of amniotic fluid volume, and the clinical significance of oligohydramnios. In addition, the literature regarding perinatal outcome associated with oligohydramnios and current available data supporting expectant noninterventional management of cases complicated by isolated oligohydramnios at term are discussed.

Amniotic fluid (AF) is a biological medium uniquely suited for the study of early exposure of the human fetus to environmental contaminants acquired by the mother before and during pregnancy. Traditional diagnostic applications of AF have focused almost exclusively on the diagnosis of genetic aberrations such as Trisomy-21 and on heritable diseases in high-risk pregnancies. Since more than 50 anthropogenic compounds have been detected in AF, there is considerable potential in utilizing fetal protein biomarkers as indicators of health effects related to prenatal toxic exposure. Here, we focus on preterm birth (PTB) to illustrate opportunities and limitations of using AF as a diagnostic matrix. Representing a pervasive public health challenge worldwide, PTB cannot be managed simply by improving hygiene and broadening access to healthcare. This is illustrated by 15-year increases of PTB in the U.S. from 1989 to 2004. AF is uniquely suited as a matrix for early detection of the association between fetal exposures and PTB due to its fetal origin and the fact that it is sampled from women who are at higher risk of PTB. This critical review shows the occurrence in AF of a number of xenobiotics, including endocrine-disrupting compounds (EDCs), which are known or may reasonably be expected to shorten fetal gestation. It is not yet known whether EDCs, including bisphenol A, phytoestrogens, and polychlorinated biphenyls (PCBs), can affect the expression of proteins considered viable or potential biomarkers for the onset of PTB. As such, the diagnostic value of AF is broad and has not yet been fully explored for prenatal diagnosis of pregnancies at risk from toxic, environmental exposures and for the elucidation of mechanisms underlying important public health challenges including PTB.

Twin gestations are at significant increased risk for adverse perinatal outcome. As a result, although prospective randomized data is lacking, increased fetal surveillance has been advocated for twins. Growth concordance is considered a reassuring sign in twins and conversely, discordancy to possibly reflect a hostile intrauterine environment at least to the smaller twin. Consequently, increased surveillance of discordant twins is commonly practiced. Monochorionic twins are at further risk for type-specific perinatal complications, for example, twin-twin transfusion syndrome. Recently, precise first-trimester depiction of chorionicity has enabled early antepartum stratification of twin gestations according to chorionicity, in comparison with previous later (mid- and third-trimester) ultrasonographic diagnosis of chorionicity. This immediately leads to the question whether antenatal testing of twins should differ according to chorionicity? Review of the literature supports that despite the existence of complications unique to monochorionic twin gestations, dichorionic twins sustain an increased risk of adverse perinatal outcome (such as fetal growth restriction) in comparison with singletons, and that close antenatal fetal surveillance of twins should be performed, irrespective of chorionicity.

Celiac disease (gluten-sensitive enteropathy) may manifest clinically with an array of nongastrointestinal symptoms among which are: dermatitis herpetiformis; dementia; depression; various neurological symptoms; osteoporosis; osteomalacia; dental enamel defects, and anemia of various types. Important data have accumulated in recent years regarding the association between celiac disease, fertility and pregnancy. Many primary care obstetricians and gynecologists and perinatologists are not aware of these important relationships. The aim of this review, utilizing a MEDLINE search from 1966 through March 2000 of the English language, is to describe the possible effects of celiac disease and its treatment upon the reproductive cycle, fertility, pregnancy, and menopause. Review of the literature reveals that patients with untreated celiac disease sustain a significantly delayed menarche, earlier menopause, and an increased prevalence of secondary amenorrhea. Patients with untreated celiac disease incur higher miscarriage rates, increased fetal growth restriction, and lower birth weights. It appears that improvement of celiac disease, as reflected by restoration of small bowel mucosa associated with implementation of a gluten-free diet, may decrease miscarriage rates, improve fetal nutritional support and overall perinatal outcome.

Pseudomyxoma peritonei is an unusual condition more common in females, in which massive amounts of mucinous ascites in conjunction with mucinous peritoneal and omental implants occur. We performed a MEDLINE search of the English-language literature from 1966 to June 2000, utilizing the key words pseudomyxoma peritonei. All case reports, series and studies regarding this condition were reviewed. Cross-referencing was also performed. The etiology and treatment of this condition are currently controversial. Most investigators agree that surgical debulking and appendectomy are adequate initial therapeutic measures. The role of intraperitoneal chemotherapy, radiotherapy or application of mucolytic therapy remains uncertain. Recent molecular genetic studies suggest the appendix as the organ of primary origin of this disease.

Background Multiple endocrine neoplasia (MEN) type 2a is an autosomal dominant syndrome caused by specific proto-oncogene mutations characterized by medullary carcinoma of the thyroid, pheochromocytoma, and, occasionally, multiglandular parathyroid hyperplasia, which rarely complicates pregnancy. Secondary diabetes rarely has been reported in association with principal endocrinopathies complicating pregnancy. Case A 34-year-old primiparous woman with recently diagnosed gestational diabetes had repeated episodes of dizziness at 30 weeks of gestation, initially attributed to glyburide. Continued episodes of dizziness and later-appearing bouts of severe headache, palpitations, diaphoresis, severe hypertension, and marked tachycardia led to diagnosis and management of MEN type 2a complicating pregnancy. Conclusion Patients with MEN type 2a complicating pregnancy may present with gestational diabetes.

Hyperemesis gravidarum or pernicious vomiting of pregnancy affects between 0.3% and 2% of all pregnant patients. The objective of this paper is to review current literature pertaining to epidemiology, etiology, symptomatology, complications, treatment, and perinatal outcome of patients with hyperemesis gravidarum. We performed a MEDLINE search of the English literature from 1966 through January 2000 utilizing the keywords: hyperemesis gravidarum, nausea and vomiting, and pregnancy. Current data pertaining to epidemiology, etiology, clinical manifestations, differential diagnosis, complications, various treatment modalities, subsequent perinatal outcome and recent developments are presented. Review of the literature supports that hyperemesis gravidarum is a multifactorial disease in which pregnancy-induced hormonal changes associated with concurrent gastrointestinal dysmotility and possible Helicobacter pylori infection function as contributing factors. Therapeutic key elements are mainly supportive in conjunction with antiemetic medication. It appears perinatal outcome is unaffected.

Placental hemosiderin deposition representing intrauterine bleeding at least 24–48 h before delivery is detected frequently in prematurity. The objective of this study was to assess incidence and site of histologic evidence of intrauterine bleeding in association with fetal growth in prematurity. Placentas of consecutive nonanomalous singleton liveborns delivered 2 weeks were excluded. A single reviewer blinded to clinical data except for gestational age at delivery assessed the presence of decidual hemosiderin. Statistical analysis included ANOVA, and Mann-Whitney U test with p < 0.05 considered significant. The study included 352 patients delivered for principal indication of premature rupture of membranes (PROM) or preterm labor (PTL) and 78 patients delivered for preeclampsia between 1989 and 1994. Mean birth weight percentiles for neonates delivered following PROM/PTL versus preeclampsia were: no decidual hemosiderin 42 ± 25 versus 17.4 ± 25, extraplacental membrane hemosiderin 42 ± 25 versus 9.2 ± 10, placental basal plate hemosiderin 42 ± 25 versus 17 ± 24, and hemosiderin in both sites 27 ± 21 versus 6.4 ± 10 (p = 0.02). Hemosiderin deposition in both placental basal plate and extraplacental decidua is associated with significantly lower mean birth weight percentiles in PROM/PTL at less than 32 weeks of gestation. We postulate that in these patients placental disruption which accompanies decidual bleeding may explain the relatively impaired fetal growth. In preeclampsia, hemosiderin depositions are not associated with further impaired fetal growth.

We present current data pertaining to angiogenesis of the ovary throughout the follicular and luteal phases of the ovarian cycle, in various ovarian pathologic conditions and in benign and neoplastic diseases. MEDLINE, Current Contents, and Index Medicus were searched for studies published between January 1966 and October 1998. All studies that incorporated human and animal models of angiogenesis of the normal ovarian physiologic state and pathologic conditions including both benign and neoplastic ovarian diseases were reviewed. Current literature supports that angiogenesis is an important component of both follicular and luteal phases of the ovarian cycle that correlates well with maturation of secretory endometrium. Angiogenesis also participates in various pathologic processes of the ovary, including follicular cyst formation, polycystic ovary, ovarian hyperstimulation syndrome, and both benign and malignant ovarian neoplasms. In the future a knowledge of specific angiogenic patterns of various pathologic processes may assist in the application of antiangiogenic medications in targeted therapy of benign and neoplastic diseases of the ovary.

First-trimester screening for fetal aneuploidy using nuchal translucency (NT), pregnancy-associated plasma protein A, free or total β-hCG, and maternal age constitutes a very effective screening test for fetal Down syndrome. We describe a case in which a patient presented at 14 weeks' gestation with an acute abdomen 1 week after first-trimester screening (including NT measurement) performed elsewhere, which was negative for trisomies 21 and 18. Sonographic examination revealed an interstitial pregnancy with a singleton fetus with present cardiac activity, which had not been noted 1 week earlier at the time of earlier transabdominal NT measurement. This case indicates that successful acquisition of a NT measurement during first-trimester screening for fetal aneuploidy does not negate the rare possibility of an unusual ectopic pregnancy. © 2008 Wiley Periodicals, Inc. J Clin Ultrasound, 2009

Cases of pelvic or abdominal abscess complicating pregnancy are rare. Noninvasive diagnostic techniques including ultrasonography, computed tomography (CT), and magnetic resonance imaging (MRI) have enabled early diagnosis of this unusual complication of pregnancy. We describe a patient in whom the diagnosis of a pelvic mass was made by sonography and MRI at 25 weeks' gestation. At laparotomy, a pelvic abscess was partially resected and drained. Two weeks later when the patient again became symptomatic, a recurrent pelvic abscess was detected by CT and treated with intravenous antibiotics and CT-guided transcatheter drainage. The patient's recovery was thereafter uncomplicated and she delivered a healthy male infant by elective repeat cesarean at 38 weeks' gestation. The literature concerning pelvic and abdominal abscesses during pregnancy is reviewed. The potential advantages of imaging-guided, percutaneous transcatheter drainage for the treatment of such patients are emphasized. Target audience Obstetricians & Gynecologists, Family Physicians. Learning objectives After completion of this article, the reader will be able to understand the nonsurgical alternatives for the drainage of intraabdominal abscesses, the possible etiologies of intraabdominal abscess formation in the pregnant population, and the various approaches to percutaneous catheter drainage of intraabdominal abscesses.

The objective of this study was to review current knowledge regarding the performance of the AutoPap 300 QC System (NeoPath Inc., Redmond, WA) for automated cervical cytology screening. To this goal, we identified all studies published in the English language that included the AutoPap 300 QC automated cervical cytology system. The studies were obtained from a MEDLINE search through October 1998; additional sources were identified through cross-referencing. Studies concerning the AutoPap 300 QC System containing complete data are presented descriptively. Meta-analyses about the performance of the AutoPap 300 QC System were performed. The central goal of the meta-analyses was to estimate the overall false-negative rate of the AutoPap 300 QC System when applied in either of the two following modalities: primary screening and quality control. Of the 14 studies concerning the performance of the AutoPap 300 QC System as a primary screening modality, four studies provided complete data about the number of abnormal slides, review rate, and number of slides selected. Meta-analysis of these four studies indicate sensitivities ranging between 85 and 100 percent. Regarding the performance of the AutoPap 300 QC System in the quality control modality, of the 14 studies reviewed, 5 studies provided complete data including the number of false-negatives, review rate, and number of slides selected. Meta-analysis of these five studies indicate an average sensitivity of the AutoPap 300 QC System applied as a rescreening modality of 37 percent (95% CI; 34-40 percent), with observed salvage ratios of between 3.5 and 5.6 when review rate of the AutoPap 300 QC System was set at 10 percent.

Objective: To present current data pertaining to angiogenesis of the endometrium throughout the normal menstrual cycle and in benign and neoplastic diseases of the endometrium. Sources: We conducted a computerized search of MEDLINE, Current Contents, and Index Medicus for relevant studies in the English literature published between January 1966 and October 1998. Study selection: We reviewed all studies that included human and animal models of angiogenesis of normal cyclic endometrium and benign and neoplastic endometrial diseases. Tabulation, Integration, and Results: Angiogenesis is important to cyclic, regenerating endometria and disease processes including dysfunctional uterine bleeding, response to exogenous hormonal treatment, bleeding associated with intrauterine contraceptive devices, uterine leiomyomata, endometriosis, complex endometrial hyperplasia, and endometrial carcinoma. Conclusion: In the future, knowledge of specific angiogenic patterns of various disease processes might improve application of antiangiogenic medications in therapies for benign and neoplastic diseases of the endometrium.

Our objective was to assess current knowledge regarding PAPNET (Neuromedical Systems, Inc.) automated cervical cytology screening methods and to assess the performance of this automated system in comparison with manual screening. To this goal, studies published in the English language regarding the PAPNET system, identified from a MEDLINE search through August 1998 were selected. Performance of the PAPNET system was assessed with various meta-analysis techniques, using the method of Mantel-Haenszel. In the primary screening modality, meta-analysis of the performance of the PAPNET system indicates that when compared with manual screening, the odds of obtaining a positive result were significantly greater. The Mantel-Haenszel odds ratio for combined studies was 1.19 (95 percent CI = 1.13 to 1.26, P.001), corresponding to 20 percent greater odds of positive or suspicious slides with PAPNET system. The PAPNET system performs with almost two-fold less false-negative results. The Mantel-Haenszel odds ratio for combined studies was 0.41 (95 percent CI = 0.25 to 0.67, P.005). Applied as a quality control modality rescreening all consecutive previously manually screened negative slides, depending on study design, the PAPNET system reclassified as abnormal between 0.1 and 5 percent. However, when the PAPNET system was used to rescreen known false-negative slides, PAPNET system rescreening can correctly identify between 20 and 90 percent of manually screened false-negative slides with an average reduction of 33 percent of the manually screened false-negative slides. We conclude that compared with manual screening, PAPNET identifies 20 percent more abnormal, has two-fold less false-negative, and reclassifies as abnormal one third of manually screened false-negative slides.ObstetriciansGynecologists, Family Physicians.After completion of this article, the reader will be able to understand how the PAPNET system works and what is its approved use by the FDA, and to understand the associated benefits and shortcomings of the PAPNET system when compared with the traditional screening method.

The differential diagnosis of prenatally diagnosed adrenal masses includes neuroblastoma, adrenal hemorrhage, adrenal and cortical renal cysts, adrenal adenoma and carcinoma, subdiaphragmatic pulmonary sequestration, Beckwith-Wiedemann syndrome, duplication of the renal system, Wilms tumors, congenital mesoblastic nephroma, and mesenteric and enteric duplication cysts. 1-5 The worldwide annual incidence of childhood adrenal cortical neoplasms ranges between 0.3 and 0.38 per 1 million children younger than 15 years. 6 These neoplasms are even more unusual among infants, with only 23 cases reported in the literature. 6 We present the first report of prenatal sonographic findings of a fetus with adrenal cortical adenoma.

We present an unusual case in which a patient with asthma presented with acute respiratory distress of acute onset, secondary to marked atelectasis of the right middle and lower lobes, which resolved within 24 hr following administration of increased doses of intravenous steroids, inhalation therapy (beta-agonists and steroids), and pulmonary physiotherapy. This transient occurrence responding to basic therapeutic measures was considered consistent with the release of a mucous plug which had caused the above obstruction and associated symptomatology and radiographic findings. This case illustrates and supports the practice of chest imaging in patients with atypical presentations of asthma and stresses the importance of pulmonary physiotherapy and bronchodilatory therapy as primary therapeutic agents in cases of mucous plug-associated atelectasis.

Objective: Ultrasound (US) is currently available on most if not all Labor and Delivery (L+D) services. Our objective was to survey utilization of real-time US on L+D in an active academic teaching hospital. Study Design: Between April 1, and July 31, 1998, all US examinations performed for clinical purposes on patients presenting to L+D, were documented. Data collected included: gestational age, whether or not the patient was in labor, number of fetuses, and indication for US. All US examinations were performed by OB/GYN housestaff at the PGY 2-3 level, and fellows in Maternal-Fetal Medicine. Statistical analysis included Student's t-test and X 2 when appropriate, with p < 0.05 considered significant throughout. Results: During the 4-month study period, 1316 patients delivered and 1363 were discharged from L+D, not in labor. Of 630 US examinations 31.64% (192 of 630) and 67.69% (418 of 630) were performed in laboring versus nonlaboring patients, respectively. Of all patients delivered during the study period, 14.5% (192 of 1316) underwent intrapartum US, and of all nonlaboring patients, 30.66% (418 of 1363) underwent US on L+D. The mean gestational age at the time of assessment was 37. 32 ± 4.23 weeks' versus 35.74 ± 5.76 weeks' gestation, in laboring versus nonlaboring patients respectively, p < 0.05. Main indications for US in patients in labor were; fetal presentation in patients with spontaneous rupture of membranes (SROM) 34.4% (n = 66), confirmation of vertex presentation 20.3% (n = 39), preterm labor 12% (n = 23), multiple gestation 7.3% (n = 14), and malpresentation 7.3% (n = 14). Main indications for patients not in labor were; amniotic fluid index 15.8% (n = 66), SROM 15.6% (n = 65), postdates 9.8% (n = 41) placental location 9.6% (n = 40), and decreased fetal movement 9.3% (n = 39). Ultrasound-guided interventions included: all deliveries of multiple gestations (n = 9), version in nonlaboring patients (n = 10), and postpartum curettage for retained placental tissue in conjuction with severe early postpartum hemorrhage (n = 2). The incidences of each separate indication for US were significantly different between laboring versus nonlaboring patients, p < 0.05, respectively. Conclusion: US examination is performed in 15% of patients in labor and 31% of patients not in labor assessed on L+D, constituting a widely applied diagnostic tool in this environment.

Glanzmann's thrombasthenia is a rare autosomal recessive bleeding disorder resulting from a deficiency of glycoprotein IIb-IIIa complex in platelets. The deficient complex normally mediates platelet aggregation by binding adhesive proteins, which form bridges between activated cells. Despite normal platelet counts, morphology, prothrombin, and activated thromboplastin times, Glanzmann's thrombasthenia is characterized by a prolonged bleeding time and a severe hemorrhagic mucocutaneous diasthesis. Pregnancy and delivery are rare in these patients and have been associated with a high risk of severe hemorrhage. We present an unusual case in which a primi-gravida patient with Glanzmann's thrombasthenia underwent an uneventful pregnancy and spontaneous vaginal delivery, following intrapartum intravenous administration of single-donor platelets. Subsequent late postpartum hemorrhage required intravenous transfusion of an additional unit of single-donor platelets. In addition, we review the literature pertaining to pregnancy and Glanzmann's thrombasthenia with an emphasis on intrapartum prophylactic management.

OBJECTIVE To evaluate whether fetal heart rate (FHR) patterns obtained in nonstress testing within 24 hours of delivery in patients with preterm delivery were associated with histologic acute infection, and if so, whether the associations are with maternal as opposed to fetal acute inflammation (acute amnionitis versus acute umbilical vasculitis). METHODS The data set included 351 consecutive patients delivering from 22 to 32 weeks' gestation (excluding cases of preeclampsia; nonhypertensive abruption; stillbirth; fetal structural and karyotypic anomalies; Rh isoimmunization and hydrops fetalis; and maternal diabetes and hypertension). Severe variable decelerations were defined as FHR 60 seconds, and decreased fetal heart variability included both reduced beat-to-beat variability and long-term heart rate cyclicity. Amniotic fluid volume was graded sonographically as part of a fetal biophysical profile. Acute inflammation of amnion (indicative of maternal inflammation) and umbilical cord (fetal inflammation) were scored by a single pathologist blinded to clinical data. RESULTS Severe FHR variable decelerations were directly related to acute amnionitis (P = .012) and acute umbilical vasculitis (P = .0013). In preterm labor, decreased FHR variability was related to acute amnionitis (P = .005). All observations were independent of amniotic fluid volume or use of tocolytic agents. CONCLUSIONS Severe variable decelerations and decreased FHR variability at < 32 weeks' gestation are related to histologic evidence of acute inflammation.

We present an unusual case, in which a woman presenting with markedly decreased fetal movements at 29 weeks gestation following a recent increase in fundal height was noted sonographically to have fetal hydrops consisting of scalp edema, marked hepatomegaly, ascites, and polyhydramnios. No lethal structural congenital anomaly was noted. Admission laboratory examinations revealed a negative antibody screen and a negative RPR. Emergent cesarean section was performed due to prolonged fetal bradycardia during biophysical profile testing. The acidotic hydropic neonate weighing 1825 g was resuscitated yet succumbed at 3 hr of life following intravenous administration of antibiotics. Neonatal blood was RPR positive at 1:16. Postmortem pathology examination demonstrated severe multiorgan system failure secondary to overwhelming congenital syphilis. Extensive extramedullary hematopoiesis was noted and histopathology with Dieterle stains revealed numerous hepatic spirochetes. Postpartum reexamination of the maternal blood with serial dilutions revealed a positive RPR at 1:1024. This case emphasizes that initial negative screening for syphilis may be seen despite overwhelming infection, a condition that has been termed the "prozone effect."

Objective: To determine whether the forewaters should be considered in the assessment of amniotic fluid (AF) volume in patients beyond 37 weeks' gestation. Methods: Sixty patients were prospectively studied and designated as having oligohydramnios or normal AF volume based upon on the standard four-quadrant AF index (AFI) of ≤5 cm or >5 cm, respectively. The distance between the internal os and the fetal head was measured transvaginally. This measurement was first added to the standard AFI and subsequently interchanged with the lowest of the two lower abdominal quadrant measurements. Statistical analysis included Student's t-test with p < 0.05 considered significant. Results: Thirty patients were classified as oligohydramnios and 30 normal AF volume. The two subgroups did not differ as to maternal age, parity, gestational age at sonographic examination, incidence of meconium-stained amniotic fluid or 5-min Apgar scores

We present an unusual case in which sonographic assessment at 33 weeks' gestation, 5 weeks following a normal fetal anatomical survey and biometry, demonstrated a large, irregular-shaped, echogenic, suprasellar midline intracranial mass occupying the anterior and middle fossas. Associated severe obstructive hydrocephalus with "dangling" choroid plexus bilaterally was noted with a markedly thin cortical mantle and increased cranial biometry. Elective cesarean delivery was performed due to the associated craniomegaly at 37 weeks' gestation. Although breathing spontaneously at delivery, the infant subsequently required mechanical ventilation and developed neonatal seizures. A ventriculoperitoneal shunt was placed on Day 3 of life. Transcranial needle biopsy demonstrated malignant astrocytoma (glioblastoma multiforme). This case suggests the rapid development of an intracranial malignant astrocytoma over a relatively short period of time. The significant ultrasonographic finding of an intracranial, destructive fetal deformation, following a normal examination 5 weeks previously, demonstrates the limitations of screening ultrasonography in predicting perinatal outcome.

Conjoined twins, rare complications of monozygotic twinning, occur in approximately 1% of monozygotic twins or between 1 per 100,000 and 1 per 250,000 live births. 1,2 Thoracopagus is the most common type of conjoined twins, with approximately 75% of such cases having conjoined hearts. 3 Reported cases of first-trimester transvaginal sonographic diagnosis of conjoined twins have almost uniformly involved gestational ages ranging between 11 and 14 weeks. 4-11 Earlier diagnosis is uncommon, yet single cases have been reported at 9 and 10 weeks, respectively. 12-17 An additional case of conjoined twins in a triplet pregnancy at 10.5 weeks' gestation was reported by Sepulveda et al. 18 We report an unusual case in which transvaginal color Doppler imaging confirmed the presence of thoracopagus, suspected during real-time sonography at 7 weeks' gestation.

Received July 13, 2006, from the Division of Maternal-Fetal Medicine, Department of Obstetrics and Gynecology, State University of New York, Downstate Medical Center, Brooklyn, New York USA. Revision requested July 18, 2006. Revised manuscript accepted for publication July 19, 2006. Address correspondence to David M. Sherer, MD, Division of Maternal-Fetal Medicine, Department of Obstetrics and Gynecology, State University of New York, Downstate Medical Center, 445 Lenox Rd, Box 24, Brooklyn, NY 11203-2098 USA. E-mail: dmsherer@aol.com Abbreviations EXIT, ex utero intrapartum treatment; 3D, 3-dimensional n 18-year-old nulliparous patient was seen for a second opinion at the State University of New York Downstate Medical Center after the diagnosis of a fetal “neck tumor” at 35 weeks’ gestation. Real-time 2-dimensional sonography and 3-dimensional (3D) sonography (Philips Medical Systems, Bothell, WA; iU22 sonography machine with a 2to 6-MHz 3D broadband curved array transducer) depicted an appropriatefor-gestational-age singleton fetus with an 11 × 10 × 9-cm (calculated volume, 900 mL) multilobulated, semisolid, semicystic mass protruding from the oral orifice (Figures 1–3). Smaller tumors of similar consistency protruded from and completely obstructed both nostrils (Figure 3). The fetal stomach was visualized, yet polyhydramnios was suggestive of impaired (obstructed) swallowing. The fetal neck and other anatomic structures appeared normal. An epignathus (congenital teratoma) involving the oropharynx and nasopharynx was diagnosed.1 Because of the anticipated neonatal upper airway obstruction at delivery, the patient was transferred to a fetal surgery center. Ultrafast fetal magnetic resonance imaging confirmed the presence of a large, multilobulated mass in the nasopharynx, oropharynx, oral cavity, and nasal cavities projecting outside the fetus. At 36 weeks’ gestation after spontaneous rupture of membranes, a female fetus weighing 2348 g was born by cesarean delivery and a successful ex utero intrapartum treatment (EXIT) procedure (tracheotomy).2 In the early neonatal period, the tumor emanating from the oral orifice (the hard palate) was partially resected. Histopathologic examination showed a teratoma. Later, the smaller nasopharyngeal and residual oropharynx tumors were resected. The infant subsequently had swallowing and feeding difficulties and severe gastroesophageal reflux. Accordingly, gastric fundoplication and gastrostomy were planned.

Hematotrachelos, engorgement of the uterine cervix with retained blood, may represent congenital or acquired conditions. Congenital hematotrachelos may be associated with obstruction distal to the cervix in cons ditions that include imperforate hymen, complete transverse vaginal septum, and, rarely, agenesis of the vagina with cervical atresia. 1-3 In contrast, acquired hematotrachelos is usually associated with cervical stenosis as a result of destructive cervical surgeries, including cone biopsy and loop electrosurgical excision procedures performed in the clinical management of cervical dysplasia. 4-8 We report an unusual case in which the development of acute spontaneous hematotrachelos was sonographically depicted in a multiparous patient during a 5-week period in proximation with intramuscular methotrexate treatment of a suspected tubal pregnancy.

Objective The objective of the study was to assess the incidence of gestational diabetes among black women according to various cut-off values of the glucose challenge test. Study design We performed a retrospective chart review of black patients who had a 50-g, 1-hour glucose challenge test between 24 and 28 weeks' gestation of 130 mg/dL or higher followed by a 100-g, 3-hour glucose tolerance test. Results were categorized in 10-mg/dL increments. Gestational diabetes was diagnosed by 2 or more abnormal values using the Carpenter-Coustan criteria. Results The study included 387 patients. The incidence of gestational diabetes diagnosed overall in all patients with a glucose challenge test of 130 mg/dL or higher was 31.2%. The incidence of gestational diabetes in individuals with a challenge test in the range of 130 to 140 mg/dL was 10.7%. The incidence of gestational diabetes with a screening test of 180 mg/dL or higher was 72.0%. Conclusion Our data support the use of 130 mg/dL as the threshold for a positive glucose challenge test and suggest the use of a glucose tolerance test to confirm the diagnosis of gestational diabetes for screening values up to 200 mg/dL.

Multiple gestations present a significant increase in fetal growth abnormalities in direct relationship to the number of fetuses present. Various definitions of birth weight discordancy exist (> or = 15%-40%). When first trimester discordancy in CRL or gestational sac diameter or early second trimester discordancies of BPD, HC, AC, or femur length are detected, genetic counseling and further work-up, including chromosomal analysis, should be considered. AC and SEFW are the best sonographic predictors of second and third trimester growth discordancy of twins. Discordant Doppler velocimetry of the umbilical arteries enhances ultrasonographic diagnosis of twin discordancy. Karyotyping also should be considered on second or third trimester diagnosis of growth discordancy. Twin-twin transfusion should be considered when growth discordancy is diagnosed in monochorionic gestations. Concordant twins with appropriate-for-gestational-age growth parameters should be followed with repeated sonographic assessment of fetal growth at approximately 4-week intervals. Discordant twins should be followed by repeat sonographic assessment of fetal growth at closer intervals, most probably every 2 weeks. Surviving singleton fetuses, after spontaneous fetal death of a twin at > 16 weeks of gestation, should be followed with antepartum surveillance similar to that of discordant twins. In rare cases of extremely premature twins with discordant growth and deteriorating fetal well-being of the growth-restricted twin, conservative management should be considered in favor of the normally grown fetus.

Our objective was to determine if placental histologic acute inflammation is related to maternal and fetal serum cytokine levels in preterm labor, using a data set previously constructed blinded to histopathologic information. To this goal in 1992, 32 consecutive patients at 20-36 weeks with progressive labor and tocolytic failure were recruited. Maternal serum sampled during the active phase of labor, and fetal (umbilical vein) serum were assayed by ELISA for levels of soluble interleukin-1 beta (IL-1 beta), soluble interleukin-2 receptor (IL-2 R), and interleukin 6 (IL-6) (T-Cell Diagnostics). Acute placental inflammation was scored by two groups blinded to clinical data, and the average scores analyzed for relationships to serum cytokine levels. Weighted kappa values, reflecting interobserver agreement in scoring of acute inflammation, were: amnion 0.84; choriodecidua 0.84; umbilical cord 0.85; and chorionic plate 0.73. Fetal levels of IL-1 beta and IL-2 R were higher with grade 3-4 acute amnionitis than with grades 0-2 (p = 0.022 and p = 0.023). Fetal levels of all three cytokines were higher in grade 3-4 umbilical vasculitis (IL-1 beta p = 0.008, IL-2 R p = 0.01, and IL-6 p = 0.03). In contrast, maternal serum cytokine levels were not associated with presence or severity of histologic evidence of acute placental inflammation. Histologic acute inflammation was not related to duration of labor, interval from membrane rupture to delivery, and presence or duration of antibiotic therapy. We conclude that fetal serum, but not maternal serum cytokine levels, are correlated with histologic evidence of acute placental inflammation, and may reflect a predominant placental origin of the cytokines.

To assess the advantage of applying uterine fundal pressure to assist transvaginal sonographic imaging of early second-trimester fetal anatomy.One hundred consecutive patients with singleton fetuses underwent routine transvaginal sonographic assessment of fetal anatomy between 13 and 17 weeks' gestation. If the entire fetal anatomy including cardiac outflow tracts was not depicted, uterine fundal pressure was applied with the operator's nonscanning hand in a bimanual fashion, to facilitate transvaginal imaging. Transabdominal sonography was performed when visualization of the entire fetal anatomy was not obtainable with transvaginal or uterine fundal pressure-assisted transvaginal sonography. Observed fetal structures with and without fundal pressure were compared. Factors assessed that may have modified the value of fundal pressure included patient weight, gestational age, fetal presentation, previous abdominal surgery, and the presence of uterine fibroids. Statistical analysis included McNemar test, chi 2, Fisher exact test, and t test, with P.05 considered significant.Visualization of lower limbs, head (including intracranial structures), upper limbs, kidneys, spine, gender, feet, hands (digits), face, four-chamber view, and cardiac outflow tracts was significantly enhanced by uterine fundal pressure-assisted versus nonassisted transvaginal sonography. Uterine fundal pressure improved transvaginal sonographic imaging in 91% of subjects, and in 51% of all subjects, a complete examination was thus obtained. In 20% of all subjects, transabdominal sonography was required to complete the examination. Complete fetal anatomic scanning was unobtainable despite uterine fundal pressure supplemented by transabdominal sonography in 29% of cases. Completion of the transvaginal sonographic fetal anatomic survey with uterine fundal pressure was related to gestational age (P.02) and maternal weight (P.05) yet not related to fetal presentation (P = .13), previous abdominal surgery (P = .06), or uterine fibroids (P = .26).Uterine fundal pressure applied during early second-trimester transvaginal sonographic evaluation of fetal anatomy significantly improves visualization of fetal structures otherwise located beyond the effective range of the transvaginal transducer.

The reported incidence of a true knot of the umbilical cord is approximately 1.2% of all deliveries. 1 , 2 Prenatal sonographic diagnoses of cases of a true knot of the umbilical cord have been reported infrequently. 3 - 6 RamOn y Cajal and Martinez 7 recently reported characteristic sonographic findings of this condition, in which a detailed investigation disclosed a transverse section of the umbilical cord surrounded by a loop of umbilical cord. This finding, noted in 5 cases, was termed the "hanging noose" sign. We present an unusual case in which 2 true knots of the umbilical cord were noted by power Doppler sonography in close proximity to each other at 33 weeks' gestation and were confirmed as separate true knots of the umbilical cord by 3-dimensional sonographic imaging.

The objective of this study was to assess the relationship between amniotic fluid volume (AFV) and fetal movements at32 weeks gestation as assessed by routine biophysical profile (BPP). From a database of 465 consecutive nonhypertensive, nondiabetic patients delivering at32 weeks gestation, patients with singleton, nonanomalous fetuses with AFV and fetal movements determined as part of a BPP assessment within 24 hours of delivery were studied. Amniotic fluid volume was scored 0 to 2, according to the following criteria: largest pocket in vertical diameter1 cm = 0;2 but1 cm = 1;or = 2 cm = 2. Fetal movements (FM) were scored over 30 minutes: 0 if absent, 1 if 1 to 2 movements, 2 ifor = 3 gross (limb/trunk) movements. Variables assessed included fetal presentation, gestational age (GA), premature rupture of membranes (PROM) as a principal indication for delivery, clinical chorioamnionitis (diagnosed by previously published criteria), histologic parameters of infection (in amnion and umbilical cord assessed by a single pathologist blinded to clinical data), and neonatal outcome. Statistical analyses included contingency tables and analysis of variance with p0.05 considered significant. Three hundred and fifty-two patients met the inclusion criteria. One hundred and sixty-seven patients (47%) had PROM as a primary indication for delivery. Infrequently, decreased fetal well-being manifested by a BPP7 of 10 points was an indication for delivery despite prematurity (n = 7). Of the 352 patients, 80 (23%) had AFV = 0, 60 (17%) had AFV = 1, and 212 (60%) had AFV = 2; and 12 (3%) had FM = 0, 30 (9%) FM = 1, and 310 (88%) FM = 2. There was a significant correlation between decreased AFV and decreased fetal movements (p0.0001). Fetal presentation and GA were not significantly different between patients based on score of fetal movements. The incidence of clinical chorioamnionitis was significantly greater in patients with FM = 0 (p0.005). We conclude that decreased AFV is associated with decreased fetal movements irrespective of fetal presentation or gestational age. Neonatal outcome (umbilical vasculitis, sepsis, intraventricular hemorrhage) is affected only in unusual cases in which otherwise uncompromised (nonhypoxic, nonacidotic) fetuses have low scores on both these antepartum ultrasonographic parameters.

We present an unusual complication of midtrimester genetic amniocentesis of a monochorionic twin gestation in which prolonged fetal bradycardia of 60 to 80 beats per minute sustained over two hours was associated with multiple subsequent fetal anomalies. These included: microcephaly, bilateral closed-lip schizencephaly, duodenal atresia, and complete paresis of the left upper extremity. Etiology of the fetal bradycardia was unclear. Fetal hemorrhage was not visualized despite targeted, continued real-time ultrasonography and was therefore considered an unlikely etiology. Although the bradycardia and associated hypoperfusion could alone explain the fetal outcome, destabilization of hemodynamics of a communicating vessel, resulting in a possible (although unproven) acute twin-twin transfusion induced at the time of amniocentesis due to decreased intra-amniotic pressure in association with a velamentous umbilical cord insertion could have led to this unusual occurrence.