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The aim of this study was to investigate the coinfection of feline retroviruses (feline immunodeficiency virus—FIV, and the feline leukemia virus—FeLV) with Leishmania infantum and Toxoplasma gondii and the factors associated with these pathogens in domestic cats from Mossoró, a city endemic for canine and human leishmaniasis situated in the semiarid region of Northeast Brazil. Blood samples from 120 cats were collected, and an epidemiological questionnaire was applied to investigate the risk factors associated with the infections. Retroviruses, L. infantum, and T. gondii infections were assessed using a point-of-care ELISA and quantitative PCR (qPCR), indirect fluorescent antibody test (IFAT) and qPCR, and IFAT, respectively. The overall seroprevalences observed were 35% (95% CI = 27.0–43.8%) for FIV, 0.8% (95% CI = 0.1–4.5%) for FeLV, 25.8% (95% CI = 18.8–34.3%) for T. gondii, and 4.2% (95% CI = 1.7–9.3%) for L. infantum. Coinfection with FIV and L. infantum was observed in 2.5% (3/120) of the assessed cats, while 12.5% (15/120) were coinfected with FIV and T. gondii. No significant association was found among the investigated agents (p > 0.05). The factors associated with FIV infection in the multivariable analysis were male sex and age above 78 months. The findings of this study demonstrated a high rate of FIV infection in cats from the Brazilian semiarid region and the exposure of these animals to zoonotic and opportunistic agents. Due to the immunosuppressive potential of FIV, cats infected with this retrovirus should be screened for coinfections with L. infantum and T. gondii, and preventative measures should be adopted. [ABSTRACT FROM AUTHOR]

Feline immunodeficiency virus (FIV) and feline leukemia virus (FeLV) are retroviruses of great importance for domestic cats with a worldwide distribution. A retrospective study was conducted to determine the epidemiological and clinicopathological aspects of the infection by FIV and FeLV in cats from the Brazilian semiarid region. Cats treated between 2011 and 2021 at the teaching veterinary hospital of the Federal Rural University of the Semi-Arid Region that were submitted to a point-of-care (POC) test to detect anti-FIV IgG antibodies and FeLV antigen were enrolled in the study. Overall, 454 cats were selected, of which 30.2% [95% CI = 26.0% – 34.3%] were FIV-positive, 1.1% [95% CI = 0.9% – 1.2%] were FeLV-positive, and 0.7% [95% CI = 0.1% – 1.3%] were coinfected by both retroviruses. No statistical association was found between the studied retroviruses (P = 0.144). Multivariable analysis detected significant associations between FIV infection and male sex [OR = 5.7, 95% CI = 3.0–10.7, P < 0.0001), age between 19 and 78 months [OR = 5.2, 95% CI = 2.2–12.1, P < 0.0001], age greater than 78 months [OR = 12.8, 95% CI = 5.1–31.9, P < 0.0001], crossbreed [OR = 4.1, 95% CI = 1.2–13.4, P = 0.021], the presence of oral disease [OR = 2.1, 95% CI = 1.3–3.4, P = 0.004], reduced red blood cell (RBC) count [OR = 3.7, 95% CI = 1.9–7.2, P < 0.0001], and an albumin:globulin (A:G) ratio lower than 0.6 [OR = 3.4, 95% CI = 1.6–7.1, P = 0.001]. No statistical analyses were performed for FeLV infection due to the low number of positive animals. In the quantitative analyses of hematological parameters, FIV-positive cats presented lower values for RBC, hemoglobin, hematocrit, lymphocytes, and platelets compared to the negative animals. In the biochemical profile, cats infected with FIV showed higher creatinine, urea, total protein, and globulin values, while lower values for albumin and A:G ratio were observed (P < 0.05). The findings of this study characterized the prevalence, clinicopathological findings, and risk factors associated with FIV and FeLV in cats from the Brazilian semiarid region. They may help support veterinary practitioners in diagnosing feline retroviruses. The FIV prevalence observed is among the highest reported in Brazil, demonstrating the need for prevention and control strategies for this retrovirus. • This is the largest survey regarding sample size for FIV and FeLV in domestic cats from the Brazilian semiarid region. • The prevalence of FIV is among the highest reported in Brazil (30.2%; 95% CI = 26.0% – 34.3%). • FeLV was observed only in 1.1% (95% CI = 0.9% – 1.2%) of the analyzed cats. • The risk factors for FIV-positivity were male sex, age greater than 19 months, crossbreed, and presence of oral disease. • Reduced red blood cell count and albumin:globulin ratio lower than 0.6 were also risk factors for FIV-positivity. [ABSTRACT FROM AUTHOR]