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The increasing demand for livestock and poultry feeds results in the lack of fish meals (FM). Poultry slaughterhouse by-product (PSB) is one promising strategy due to its high protein content despite the limited content of lysine. Thus, supplementing lysine in dietary fish feed is necessary. The present study aimed to investigate how different lysine doses in feed with PSB and FM as animal protein sources affected protein digestibility, feed utilization, growth, hematology, and body composition of Sangkuriang catfish (Clarias gariepinus var. Sangkuriang). Sangkuriang catfish at the grow-out stage (15.54±0.17 g/fish) were used. The fish were fed six experimental diets with similar protein and energy content but different lysine levels at 1.25%, 1.75%, 2.25%, 2.75%, 3.25%, and 3.75%/kg (treatments 1 to 6). The addition of lysine to feed had a significant (P<0.05) effect on protein digestibility (ADCp), efficiency of feed utilization (EFU), and relative growth rate (RGR) of Sangkuriang catfish at a grow-out stage but had no significant (P>0.05) effect on survival rate, hematology, and nutrient content. The optimal doses of dietary lysine with PSB and FM to improve ADCp, EFU, and RGR of Sangkuriang catfish were 2.59%, 2.63%, and 2.62%/kg diet, respectively. However, the supplementation of PSB in experimental diets had no significant effect on glucose, triglyceride, total protein, urea, calcium, magnesium, albumin, globulin, hemoglobin, hematocrit, phosphorous, and mean corpuscular hemoglobin concentration (MCHC). The lysine addition in feed formulated with PSB and FM could improve the growth performance and increase the feed digestibility of Sangkuriang catfish at the grow-out stage. [ABSTRACT FROM AUTHOR]

The high consumer demand in Indonesia encourages catfish farmers to conduct an intensive culture. A low feed efficiency mainly occurs in cultivating Sangkuriang catfish resulting in poor growth. This condition might be caused by low lysine content, as lysine is an essential amino acid that the fish cannot synthesize. The present study aimed to investigate the effect of lysine supplementation in feed on protein digestibility, feed efficiency, and growth of Sangkuriang catfish (Clarias gariepinus var. Sangkuriang) fingerlings. The study used 270 Sangkuriang catfish with an average wet weight of 7.54 ± 0.13 g/fish. The experimental feed contained protein, energy, and amino acid, and then various doses of lysine were added to the experimental feed: (1) 0.0%, (2) 0.5%, (3) 1.0%, (4) 1.5%, (5) 2.0%, and (6) 2.5%. Weight gain (WG), protein digestibility (ADCp), the efficiency of feed utilization (EFU), relative growth rate (RGR), feed conversion ratio (FCR), protein efficiency ratio (PER), and protein retention (PR) of catfish were evaluated for 8 weeks. The results found that the supplementation of lysine in feed significantly (P < 0.05) influenced WG, ADCp, EFU, RGR, FCR, PER, and PR of Sangkuriang catfish fingerling. However, there was no significant effect (P > 0.05) on the SR of Sangkuriang catfish fingerling. The supplementation of 1% lysine/kg feed was the optimal dose to improve the feed efficiency and growth of Sangkuriang catfish fingerlings by 83.79% and 3.94%/day, respectively. Therefore, the supplementation of lysine could increase Sangkuriang catfish production. [ABSTRACT FROM AUTHOR]

The success of the intensive culture of Sangkuriang catfish (Clarias gariepinus var. Sangkuriang) highly depends on the availability of feed. The feed is the most significant share of cost production hamper (50-60%). Therefore, it is suggested that feed utilization is inefficient. One of the solutions is to enrich the commercial feed with yeast (Saccharomyces cerevisiae). The research aimed to identify the impacts of yeast (S. cerevisiae) enhanced feed on feed efficiency, growth, and survival rate of Sangkuriang catfish fingerlings. The treatments research was yeast (S. cerevisiae) enrichment in the commercial feed at the various dosages: 0 %/kg feed (A), 3 %/kg feed (B), 6 %/kg feed (C), 9 %/kg feed (D), and 12 %/kg feed (E). The yeast (S. cerevisiae) enrichment in the commercial feed increased feed efficiency and survival rate of Sangkuriang catfish fingerlings. The optimum dosage of S. cerevisiae for apparent digestibility coefficients for protein (ADCp), efficiency feed utilization (EFU), protein efficiency ratio (PER), and relative growth rate (RGR) ranged from 6.10% to 6.51%/kg feed. [ABSTRACT FROM AUTHOR]