1. A protocol combining breath testing and
- Author
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Laura, Payling, Nicole C, Roy, Karl, Fraser, Simon M, Loveday, Ian M, Sims, Peter H, Janssen, Stefan J, Hill, Laura G, Raymond, and Warren C, McNabb
- Subjects
Feces ,Breath Tests ,Cell Culture ,digestive, oral, and skin physiology ,Protocol ,Humans ,Genomics ,Clinical Protocol ,Gastrointestinal Microbiome - Abstract
Summary This protocol describes the application of breath testing and ex vivo fermentations to study the association between breath methane and the composition and functionality of the gut microbiome. The protocol provides a useful systems biology approach for studying the gut microbiome in humans, which combines standardized methods in human breath testing and fecal sampling. The model described is accessible and easy to repeat, but its relative simplicity means that it can deviate from human physiological conditions., Graphical abstract, Highlights • A systems biology approach for studying the human gut microbiome • Participants are breath tested and fecal samples are collected • Fecal samples are used to inoculate an ex vivo model of the colon • Samples are taken over time and analyzed using a multi-omics approach, This protocol describes the application of breath testing and ex vivo fermentations to study the association between breath methane and the composition and functionality of the gut microbiome. The protocol provides a useful systems biology approach for studying the gut microbiome in humans, which combines standardized methods in human breath testing and fecal sampling. The model described is accessible and easy to repeat, but its relative simplicity means that it can deviate from human physiological conditions.
- Published
- 2021