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Your search keyword '"Alves, Even Herlany Pereira"' showing total 4 results
Start Over Author "Alves, Even Herlany Pereira" Topic fatty liver
4 results on '"Alves, Even Herlany Pereira"'

1. El modelo experimental de dieta alta en grasas es útil para evaluar el agravamiento del daño hepático asociado a comorbilidades

Author

Rodrigues, Ayane Araújo, Alves, Even Herlany Pereira, Andrade, Raissa Silva Bacelar de, França, Luiz Felipe de Carvalho, Pessoa, Larissa dos Santos, Silva, Felipe Rodolfo Pereira da, Lenardo, David Di, Nascimento, Hélio Mateus Silva, Carvalho, André dos Santos, Sousa, Francisca Beatriz de Melo, Martins, Victor Brito Dantas, Barbosa, André Luiz dos Reis, Medeiros, Jand-Venes Rolim, Vasconcelos, Any Carolina Cardoso Guimarães, and Vasconcelos, Daniel Fernando Pereira

Subjects
Fatty liver, Non-alcoholic fatty liver disease, Hígado graso, Modelos animales, Hepatopatia gordurosa não alcoólica, Dieta, Fígado gorduroso, Enfermedad del hígado graso no alcohólico, Modelos animais, Animal models, Diet
Abstract

Objetive: Develop a high-fat diet (HFD) model that standing alone did not develop the Non-alcoholic fatty liver disease (NAFLD), allowing one to study the association of comorbidities with the high-fat diet model. Material and methods: The rats were divided into 2 groups: standard diet and high-fat diet, each group with 8 animals. The rats were submitted to the analysis of the following parameters in hepatic tissue: dosage of malondialdehyde (MDA), glutathione (GSH) and myeloperoxidase activity (MPO). Liver samples were also assessed histopathologically for serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), albumin (ALB), alkaline phosphatase (FAL), uric acid and total cholesterol (TC), calcium (CA), urea and HDL. Results: The results showed that there was a significant difference in MDA, GSH, total cholesterol (CT), ALT, ALB, uric acid (AU), calcium (CA) and HDL. The histopathological evaluation presented a low score, insufficient for the classification of NAFLD. Conclusion: This study demonstrated that the high-fat diet model did not cause NAFLD. This finding allows one to use the high-fat diet characterized in this study to investigate the possible hepatic alterations caused by other comorbidities. Objetivo: Desarrollar un modelo de dieta alta en grasas (HFD) que por sí solo no desarrolle la enfermedad del hígado graso no alcohólico (DHGNA), lo que permite estudiar la asociación de comorbilidades con el modelo de dieta alta en grasas. Material y métodos: Las ratas se dividieron en 2 grupos: dieta estándar y dieta alta en grasas, cada grupo con 8 animales. Las ratas fueron sometidas al análisis de los siguientes parámetros en tejido hepático: dosificación de malondialdehído (MDA), glutatión (GSH) y actividad mieloperoxidasa (MPO). También se evaluaron histopatológicamente muestras de hígado para determinar los niveles séricos de alanina aminotransferasa (ALT), aspartato aminotransferasa (AST), albúmina (ALB), fosfatasa alcalina (FAL), ácido úrico y colesterol total (TC), calcio (CA), urea y HDL. . Resultados: Los resultados mostraron que hubo una diferencia significativa en MDA, GSH, colesterol total (CT), ALT, ALB, ácido úrico (AU), calcio (CA) y HDL. La evaluación histopatológica presentó una puntuación baja, insuficiente para la clasificación de EHGNA. Conclusión: Este estudio demostró que el modelo de dieta alta en grasas no causó NAFLD. Este hallazgo permite utilizar la dieta rica en grasas caracterizada en este estudio para investigar las posibles alteraciones hepáticas provocadas por otras comorbilidades. Objetivo: Desenvolver um modelo de dieta rica em gordura (DRG) que sozinho não resultou em doença hepática gordurosa não alcoólica (DHGNA), permitindo estudar a associação de comorbidades com o modelo de dieta rica em gordura. Material e métodos: Os ratos foram divididos em 2 grupos: dieta padrão e dieta rica em gordura, cada grupo com 8 animais. Os ratos foram submetidos à análise dos seguintes parâmetros no tecido hepático: dosagem de malondialdeído (MDA), glutationa (GSH) e atividade da mieloperoxidase (MPO). As amostras de fígado também foram avaliadas histopatologicamente quanto aos níveis séricos de alanina aminotransferase (ALT), aspartato aminotransferase (AST), albumina (ALB), fosfatase alcalina (FAL), ácido úrico e colesterol total (TC), cálcio (CA), ureia e HDL. Resultados: Mostraram que houve diferença significativa em MDA, GSH, colesterol total (CT), ALT, ALB, ácido úrico (AU), cálcio (CA) e HDL. A avaliação histopatológica apresentou escore baixo, insuficiente para a classificação da DHGNA. Conclusão: Este estudo demonstrou que o modelo de dieta rica em gordura não causou DHGNA. Esse achado permite utilizar a dieta hiperlipídica caracterizada neste estudo para investigar as possíveis alterações hepaticas causadas por outras comorbidades.

Published
2021

2. Food restriction reduces hepatic alterations associated with experimental periodontitis.

Author

Caetano, Vinícius da Silva, de Andrade, Raissa Silva Bacelar, França, Luiz Felipe de Carvalho, Pessoa, Larissa dos Santos, Rodrigues, Ayane Araújo, Alves, Even Herlany Pereira, Lenardo, David Di, Nascimento, Hélio Mateus Silva, Ayala, Karen Neisman Rodriguez, Carvalho, André dos Santos, Brito, Tarcísio Vieira, Barbosa, André Luiz dos Reis, Vasconcelos, Any Carolina Cardoso Guimarães, and Vasconcelos, Daniel Fernando Pereira

Abstract

Background: Periodontitis is a chronic inflammatory and multifactorial disease that affects the periodontal structures and can cause alterations in the hepatic tissue. The aim of the present study was to evaluate whether a diet with food restriction can decrease oral and liver alterations associated with ligature-induced periodontitis.Methods: Twenty-four female Wistar rats were used in this study, randomized into three groups (n = 8 for each group): control (regular food); periodontitis (regular food + periodontitis induced with ligatures); and food restriction (diet with food restriction and periodontitis induction). The following periodontium parameters were analyzed tooth mobility (TM), probing pocket depth (PPD), gingival bleeding index (GBI), and alveolar bone height (ABH). In the liver, the levels of oxidative stress markers-malondialdehyde (MDA), glutathione (GSH), total cholesterol, and levels of myeloperoxidase (MPO) activity were measured. Liver samples were analyzed for histopathological score. In the blood tissue, the levels of enzymes alanine aminotransferase (ALT), aspartate aminotransferase (AST), glucose, total cholesterol, and the high-density lipoprotein (HDL) were also evaluated.Results: The animals that received a diet with food restriction + periodontitis showed a decrease in hepatic histopathological score (P < 0.05) when compared with the periodontitis group, the same for glucose, total cholesterol, ALT, AST, and ABH data. The group with food restriction + periodontitis showed a decrease in the histopathological liver score (P < 0.05) compared with the group with periodontitis.Conclusion: This study revealed that food restriction reduced oral damages, as well as hepatic, blood and alveolar bone alterations associated with ligature-induced periodontitis in rats. [ABSTRACT FROM AUTHOR]

Published
2022
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3. High-fat diet aggravates the liver disease caused by periodontitis in rats.

Author

Andrade, Raissa Silva Bacelar de, França, Luiz Felipe de Carvalho, Pessoa, Larissa dos Santos, Landim, Bruno de Almeida Arrais, Rodrigues, Ayane Araújo, Alves, Even Herlany Pereira, Lenardo, David Di, Nascimento, Hélio Mateus Silva, Sousa, Francisca Beatriz de Melo, Barbosa, André Luiz dos Reis, Medeiros, Jand‐Venes Rolim, Vasconcelos, Any Carolina Cardoso Guimarães, Vasconcelos, Daniel Fernando Pereira, and Medeiros, Jand-Venes Rolim

Abstract

Background: Periodontitis is an inflammatory disease that causes periodontium and hepatic alterations. Liver disease is related to the intake of foods rich in fat and sugars (high-fat). The objective of this study was to evaluate whether a high-fat diet can aggravate the liver disease caused by ligature-induced periodontitis in rats.Methods: Twenty-one female rats were divided into three groups (n = 7 in each group): control; periodontitis (periodontitis induced with ligature) and high-fat + periodontitis (received hypercaloric diet and induction of periodontitis). The rats were submitted to the analyses of the following periodontal parameters: gingival bleeding index (GBI), probing pocket depth (PPD), tooth mobility (TM), and alveolar bone height. In the hepatic tissue, the levels of malondialdehyde (MDA), glutathione (GSH), total cholesterol, and myeloperoxidase activity (MPO) were measured. Liver samples were also histopathologically evaluated. Finally, blood levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), glucose, total cholesterol, cholesterol high-density lipoprotein (HDL), and uric acid were measured.Results: The high-fat + periodontitis group presented an increase in the steatosis score (P < 0.05) for the histopathologic evaluation, when compared with the periodontitis group. MDA, uric acid and ALT levels also increased, whereas GSH and HDL levels showed lower values.Conclusion: A high-fat diet aggravates the liver disease caused by ligature-induced periodontitis in rats. [ABSTRACT FROM AUTHOR]

Published
2019
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4. One or two ligatures inducing periodontitis are sufficient to cause fatty liver.

Author

Santos Pessoa, Larissa-dos, Pereira-da Silva, Felipe-Rodolfo, Alves, Even-Herlany-Pereira, Carvalho França, Luiz-Felipe-de, di Lenardo, David, Santos Carvalho, Joaquina-dos, Martins, Victor-Brito-Dantas, Melo Sousa, Francisca-Beatriz-de, Drumond, Karina-Oliveira, Medeiros, Jand-Venes-Rolim, de Oliveira, Jefferson-Soares, and Vasconcelos, Daniel-Fernando-Pereira

Subjects
ASPARTATE aminotransferase, FATTY liver, PERIODONTITIS, ALANINE aminotransferase, TOOTH mobility, BONES
Abstract

Background: Periodontitis is a chronic disease that due to an intense inflammatory response triggers systemic changes such as hepatic alterations. This study aimed to compare hepatic damage in rats that received experimental periodontitis at one or two periodontal sites with ligatures. Material and Methods: Eighteen rats were separated into three groups: control, without ligature; periodontitis 1, with one ligature; and periodontitis 2, with two ligatures. The following parameters were assessed: gingival bleeding index, probing pocket depth, tooth mobility, alveolar bone loss, malondialdehyde (MDA) and myeloperoxidase (MPO) activity in periodontal tissue; histopathological evaluation of hepatic tissue (steatosis score); glutathione levels (GSH), MDA, MPO, cholesterol and triglycerides in the liver; and serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST). Results: Periodontal evaluation data showed that the periodontitis model worked well. The groups with periodontitis did not differ significantly in relation to MPO activity and MDA levels in the gingival samples, but they were significantly different when compared with the control group. Steatosis was observed in the histological analysis of the groups with periodontitis, but between the periodontitis groups, two ligatures did not cause increase in steatosis score. The levels of GSH, MDA, total cholesterol and triglycerides in the hepatic tissue were not altered between groups with periodontitis, but they showed significant differences in comparison with the control group. The activity of MPO in hepatic tissue and serum levels of AST and ALT did not present significant difference among the three groups. Conclusions: In conclusion, our results demonstrated that one or two ligatures inducing periodontitis were both sufficient to cause fatty liver. Steatosis caused by two ligatures did not present larger extension and severity than steatosis caused by one ligature. [ABSTRACT FROM AUTHOR]

Published
2018
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