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Emulsion oil-in-water was prepared with combination of emulsifiers (non-ionic surfactants) following the HLB (hydrophylic-lipophylic balance) method developed by Griffin. The emulsions were prepared at HLB 10, 11, 12, 13 and 13.6 consisting blend of non-ionic emulsifiers fatty acid ethoxylate with 20 moles bound ethylene oxide and Dehydol LS 1 with 1 mole bound ethylene oxide. A mixture of palm-based methyl ester consisting of C6-10 and C12-18 fatty acid composition was used as palm-based solvent. The physicochemical parameters of the emulsion were characterized by accelerate stability tested at 45°C for two months, measurement of particle size and viscosity test. The result of accelerate test showed that all the emulsion at different HLB were found to be stable in the 2 months observation which assumed to be stable in 1 year of storage. Meanwhile, the particle size measurement data showed that the optimum stable particle size of the emulsion was HLB 12±1. The viscosity test of the emulsion tends to support the data from the particle size and have maximum viscosity 189.89 cP at HLB 12. The obtained results indicate that the optimum stable emulsions can be formulated by a combination of emulsifiers with HLB 12±1 which is compatible with that of required HLB of the oil phase. [ABSTRACT FROM AUTHOR]