1. Sphingomonas lacusdianchii sp. nov., an attached bacterium inhibited by metabolites from its symbiotic cyanobacterium.
- Author
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Wang X, Xiao Y, Deng Y, Sang X, Deng QL, Wang L, Yang YW, Zhang BH, and Zhang YQ
- Subjects
- China, Phospholipids analysis, Microcystis genetics, Microcystis metabolism, Microcystis growth & development, Lakes microbiology, Sequence Analysis, DNA, Bacterial Typing Techniques, Symbiosis, Ubiquinone, Sphingomonas metabolism, Sphingomonas genetics, Sphingomonas isolation & purification, Sphingomonas classification, RNA, Ribosomal, 16S genetics, Phylogeny, Fatty Acids metabolism, DNA, Bacterial genetics, Base Composition
- Abstract
An alpha-proteobacterial strain JXJ CY 53
T was isolated from the cyanosphere of Microcystis sp. FACHB-905 (MF-905) collected from Lake Dianchi, China. JXJ CY 53T was observed to be an aerobic, Gram-stain-negative, oval shaped, and mucus-secreting bacterium. It had C18:1 ω7c and C16:0 as the major cellular fatty acids, Q-10 as the predominant ubiquinone, and sphingoglycolipid, diphosphatidylglycerol, phosphatidylcholine, and phosphatidylmethylethanolamine as the polar lipids. The G + C content of DNA was 65.85%. The bacterium had 16S rRNA gene sequence identities of 98.9% and 98.7% with Sphingomonas panni DSM 15761T and Sphingomonas hankookensis KCTC 22579T , respectively, while less than 97.4% identities with other members of the genus. Further taxonomic analysis indicated that JXJ CY 53T represented a new member of Sphingomonas, and the species epithet was proposed as Sphingomonas lacusdianchii sp. nov. (type strain JXJ CY 53T = KCTC 72813T = CGMCC 1.17657T ). JXJ CY 53T promoted the growth of MF-905 by providing bio-available phosphorus and nitrogen, plant hormones, vitamins, and carotenoids. It could modulate the relative abundances of nonculturable bacteria associated with MF-905 and influence the interactions of MF-905 and other bacteria isolated from the cyanobacterium, in addition to microcystin production characteristics. Meanwhile, MF-905 could provide JXJ CY 53T dissolved organic carbon for growth, and control the growth of JXJ CY 53T by secreting specific chemicals other than microcystins. Overall, these results suggest that the interactions between Microcystis and its attached bacteria are complex and dynamic, and may influence the growth characteristics of the cyanobacterium. This study provided new ideas to understand the interactions between Microcystis and its attached bacteria. KEY POINTS: • A novel bacterium (JXJCY 53T ) was isolated from the cyanosphere of Microcystis sp. FACHB-905 (MF-905) • JXJCY 53T modulated the growth and microcystin production of MF-905 • MF-905 could control the attached bacteria by specific chemicals other than microcystins (MCs)., (© 2024. The Author(s).)- Published
- 2024
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