1. Cohnella cholangitidis sp. nov., a novel species of the genus Cohnella isolated from a clinical specimen in Korea.
- Author
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Kim JK, Choi CH, Kim DW, Kim SY, Hwang KJ, Lee WK, Shin MK, Jung M, and Choi YS
- Subjects
- Bacillales, Bacterial Typing Techniques, DNA, Bacterial genetics, Humans, Phylogeny, RNA, Ribosomal, 16S genetics, Republic of Korea, Sequence Analysis, DNA, Vitamin K 2, Fatty Acids analysis, Phospholipids analysis
- Abstract
A Gram-positive, aerobic, rod-shaped bacterium, designated as strain 1605-214
T , was isolated from the blood sample of a patient with cholangitis. Based on its 16S rRNA gene sequence, the strain 1605-214T belonged to the genus Cohnella and exhibited 97.9% sequence identity with Cohnella luojiensis DSM 24270T (GQ214052). DNA-DNA hybridization, digital DNA-DNA hybridization, and average nucleotide identity values between the two species were 23% ± 1.9, 21.1%, and 77.2%, respectively. The cellular fatty acids of strain 1605-214T were mainly comprised of anteiso-C15:0 (36.1%), iso-C16:0 (16.5%), and C16:0 (15.1%). The predominant quinone was menaquinone-7; predominant polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, and aminophospholipid-1. The cell wall peptidoglycan of strain 1605-214T contained meso-diaminopimelic acid. DNA G + C content of strain 1605-214T was 50.6 mol%. 5187 genes out of a total of 5413 (94.6%) were assigned putative functions using eggNOG v5.0. Based on genotypic characteristics and genomic sequence analysis results, strain 1605-214T was confirmed to represent a novel species of genus Cohnella, for which the name Cohnella cholangitidis sp. nov., was proposed., (© 2021. The Author(s).)- Published
- 2021
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